Lentivirus介导表达多基因的人胚基因工程神经干细胞的实验研究

目的:探索以Lentivirus为载体,构建同时携带并表达多基因的基因工程人胚神经干细胞(hum an neu鄄ral stem cell,hNSC)的可行性,为脊髓损伤治疗的研究提供材料。方法:培养和鉴定hNSC;用携带绿色荧光蛋白(green fluorescence protein,GFP)和神经营养因子-3(neurotrophic factor-3,NT-3)的Lentivirus转染hNSC;用荧光显微镜观察、鼠胚背根神经结培养(dorsal root ganglion,DRG)和Slot blot等方法检测基因工程hNSC的多基因表达情况。结果:培养获得了大量的hNSC;荧光显微镜观察到几乎100%的hNSC表达GFP;基因工程hNSC的培养液能促使大鼠DRG旺盛生长;Slot blot检测到基因工程hNSC能高效分泌NT-3蛋白。结论:以Lentivirus为载体能构建同时携带并稳定表达多基因的基因工程hNSC,为脊髓损伤治疗的基础研究及进一步临床应用提供了有价值的细胞资源。

苯并(a)芘致毒的鱼的分子生态毒理学指标研究

通过测定典型的多环芳烃类物质苯并（a）芘（BaP）致毒后鱼体内几种重要分子生态毒理学指标的变化，来反映苯并（a）芘致毒对鱼体的影响．结果表明，肝脏ATPase活性降低，GST活性升高，DNA加合物相对标记水平（RAL）也增大，而EROD活性没有明显改变．这说明苯并（a）芘致毒对鱼体正常生命活动产生了重大影响，并具有潜在的致癌性．

High-speed electrooptical VOA integrated in silicon-on-insulator

In this paper, we present simulation results of an electrooptical variable optical attenuator (VOA) inte-grated in silicon-on-insulator waveguide. The device is functionally based on free carriers absorption toachieve attenuation. Beam propagation method (BPM) and two-dimensional semiconductor device simu-lation tool PISCES-Ⅱ were used to analyze the dc and transient characteristics of the device. The devicehas a response time (including rise time and fall time) less than 200 ns, much faster than the thermoopticand micro-electromechanical systems (MEMSs) based VOAs.

仿生模式识别在单镜头人脸识别中的应用

提出了一种基于仿生模式识别（Biomimatic Pattern Recognition）和多权值神经元网络(Multi-Weights.Neu-ral Network)的人脸识别新方法．对仿生模式识别理论在人脸识别中的应用模型作了讨论，并且介绍了一种新的人脸特征提取方法．本文通过实验对本文提出的基于仿生模式识别的方法和基于K近邻的方法做了对比，实验结果表明本文的方法克服了对未训练类型的人脸误识问题，提高了人脸识别系统的训练速度和正确识别率．

Preservação de germoplasma vegetal, com ênfase na conservação in vitro de variedades de mandioca.

O melhoramento genético de plantas tem contribuído sobremaneira para o aumento da produção em diversas espécies de grande importância econômica e/ou social, beneficiando bilhões de pessoas, especialmente de menor poder aquisitivo, que vivem em países em desenvolvimento distribuídos por todo o mundo. Esses aumentos na produção resultam da obtenção de novos genótipos, que apresentam rendimentos mais elevados, adaptados a diversas condições ecológicas, muitas das vezes adversas, e resistentes a pragas e doenças. No entanto, para a geração desses materiais melhorados, torna-se necessário que características de interesse sejam incorporadas às novas cultivares, dentro de programas de melhoramento genético bem definidos, de forma que possam ser, ao final de todo o processo, exploradas comercialmente.

Diagnóstico ambiental do município de Santana do Ipanema, Alagoas.

2005

Avaliação de genótipos de girassol na região do Planalto Médio do Rio Grande do Sul na safra 2005/2006.

2007

Contribuições da Embrapa Trigo e da Embrapa Transferência de Tecnologia ao desenvolvimento sustentável da reforma agrária no Rio Grande do Sul - ações e atividades realizadas de 2005 a 2008.

2009

Avaliação de genótipos de girassol no Planalto Médio do Rio Grande do Sul na safra 2008/2009.

2009

Hora da colheita: hora de cuidar do seu produto e de você. Estação de trabalho.

Na presente publicação, são apresentados um conjunto de equipamentos que facilitam e aumentam o rendimento das operações de colheita e beneficiamento de hortaliças. O conjunto é adequado para pequenas propriedades rurais onde não seja viável economicamente a construção de uma casa de embalagem de alvenaria, ou em propriedades maiores que precisem de uma infraestrutura de apoio junto à lavoura, para posterior remoção dos produtos colhidos para a casa de embalagem onde são executadas operações mais complexas de beneficiamento, embalagem e armazenamento.

Choking RECtified: embodied expertise beyond Dreyfus

On a Dreyfusian account performers choke when they reflect upon and interfere with established routines of purely embodied expertise. This basic explanation of choking remains popular even today and apparently enjoys empirical support. Its driving insight can be understood through the lens of diverse philosophical visions of the embodied basis of expertise. These range from accounts of embodied cognition that are ultra conservative with respect to representational theories of cognition to those that are more radically embodied. This paper provides an account of the acquisition of embodied expertise, and explanation of the choking effect, from the most radically enactive, embodied perspective, spelling out some of its practical implications and addressing some possible philosophical challenges. Specifically, we propose: (i) an explanation of how skills can be acquired on the basis of ecological dynamics; and (ii) a non-linear pedagogy that takes into account how contentful representations might scaffold skill acquisition from a radically enactive perspective.

Replicación Activa en NodeJS con 0MQ

Mi trabajo final de grado se centra en el aprendizaje, desarrollo y divulgación del entorno de programación NodeJS, un entorno orientado a eventos que está basado en el lenguaje de programación JavaScript en el lado del servidor. El enfoque de NodeJS es el desarrollo de aplicaciones de red altamente escalables. La escalabilidad se proporciona por la eficiencia obtenida a lo largo de los años en la optimización de código en los motores de JavaScript por la delegación asíncrona a tareas propias del sistema operativo. Aprovechando estas cualidades, desarrollo la implementación de un algoritmo basado en el modelo de replicación activa consistente en mantener copias de objetos de datos en distintos almacenes de datos. Con este modelo se constituye un sistema distribuido tolerante a fallos y de alta disponibilidad. Para los protocolos de comunicación se utilizan de los socket 0MQ y se desarrollan algunos ejemplos del uso de cada tipo de ellos.

Documentos técnicos de la propuesta para el mejoramiento tecnológico de la cerámica artesanal en la Chamba – Tolima en los procesos de extracción/beneficio de materias primas, fabricación y cocción de las piezas.

190 hojas : ilustraciones.

Dotación del taller cerámico para la vereda Patio Bonito sector el Silencio municipio de Nemocón.

6 hojas.

A GLOBAL SAMPLING OF PIANO MUSIC FROM 1978 TO 2005

Pianists of the twenty-first century have a wealth of repertoire at their fingertips. They busily study music from the different periods -- Baroque, Classical, Romantic, and some of the twentieth century -- trying to understand the culture and performance practice of the time and the stylistic traits of each composer so they can communicate their music effectively. Unfortunately, this leaves little time to notice the composers who are writing music today. Whether this neglect proceeds from lack of time or lack of curiosity, I feel we should be connected to music that was written in our own lifetime, when we already understand the culture and have knowledge of the different styles that preceded us. Therefore, in an attempt to promote today’s composers, I have selected piano music written during my lifetime, to show that contemporary music is effective and worthwhile and deserves as much attention as the music that preceded it. This dissertation showcases piano music composed from 1978 to 2005. A point of departure in selecting the pieces for this recording project is to represent the major genres in the piano repertoire in order to show a variety of styles, moods, lengths, and difficulties. Therefore, from these recordings, there is enough variety to successfully program a complete contemporary recital from the selected works, and there is enough variety to meet the demands of pianists with different skill levels and recital programming needs. Since we live in an increasingly global society, music from all parts of the world is included to offer a fair representation of music being composed everywhere. Half of the music in this project comes from the United States. The other half comes from Australia, Japan, Russia, and Argentina. The composers represented in these recordings are: Lowell Liebermann, Richard Danielpour, Frederic Rzewski, Judith Lang Zaimont, Samuel Adler, Carl Vine, Nikolai Kapustin, Akira Miyoshi and Osvaldo Golijov. With the exception of one piano concerto, all the works are for solo piano. This recording project dissertation consists of two 60 minute CDs of selected repertoire, accompanied by a substantial document of in-depth program notes. The recordings are documented on compact discs that are housed within the University of Maryland Library System.