930 resultados para Capsule vidéo


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Cytotoxicity and subcutaneous tissue reaction of innovative blends composed by polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene associated with natural polymers (natural rubber and native starch) forming membranes were evaluated, aiming its applications associated with bone regeneration. Cytotoxicity was evaluated in mouse fibroblasts culture cells (NIH3T3) using trypan blue staining. Tissue response was in vivo evaluated by subcutaneous implantation of materials in rats, taking into account the presence of necrosis and connective tissue capsule around implanted materials after 7, 14, 21, 28, 35, 60, and 100 days of surgery. The pattern of inflammation was evaluated by histomorphometry of the inflammatory cells. Chemical and morphological changes of implanted materials after 60 and 100 days were evaluated by Fourier transform infrared (FTIR) absorption spectroscopy and scanning electron microscopy (SEM) images. Cytotoxicity tests indicated a good tolerance of the cells to the biomaterial. The in vivo tissue response of all studied materials showed normal inflammatory pattern, characterized by a reduction of polymorphonuclear leukocytes and an increase in mononuclear leukocytes over the time (p < 0.05 Kruskal-Wallis). On day 60, microscopic analysis showed regression of the chronic inflammatory process around all materials. FTIR showed no changes in chemical composition of materials due to implantation, whereas SEM demonstrated the delivery of starch in the medium. Therefore, the results of the tests performed in vitro and in vivo show that the innovative blends can further be used as biomaterials. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 1284-1293, 2013. Copyright © 2013 Wiley Periodicals, Inc.

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A new species of Myxosporea, Henneguya nagelii n. sp., is described parasitizing the gills of Cyphocharax nagelii collected from Peixe's River, São Paulo State, Brazil. Among the fish examined, 16.7 % had gills parasitized by myxosporeans. The plasmodia were white, round, or oval and measured 150-250 μm. The mature spores were fusiform and had smooth wall. The spores measurements were the following: total length, 34.5 ± 4.2 (26.4-39.9) μm; body length, 12.0 ± 0.5 (11.2-11.9) μm; body width, 4.9 ± 0.3 (4.4-5.5) μm; and caudal process length, 22.4 ± 4.0 (14.7-27.3) μm. The polar capsules were elongated and of unequal size, with lengths of 4.9 ± 0.4 (4.0-5.9) μm and 5.2 ± 0.4 (4.6-6.0) μm for the longest and shortest axes, respectively. Capsule width was 1.8 ± 0.2 (1.5-2.2) μm. Each capsule contained a polar filament with six to eight turns. There was no mucous envelope or iodinophilous vacuole. Morphometric differences between this parasite and other species of the genus Henneguya indicated that the parasite observed in C. nagelii is a new species. This is the first species of Myxosporea described in Peixe's River. © 2013 Springer-Verlag Berlin Heidelberg.

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Pós-graduação em Alimentos e Nutrição - FCFAR

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Microbiologia - IBILCE