Factors influencing the stability of cyclotides: Proteins with a circular backbone and cystine knot motif

Cyclotides are a large family of mini-proteins that have the distinguishing features of a head-to-tail cyclised backbone and a cystine knot formed by six conserved cysteine residues. They are present in plants from the Rubiaceae, Violaceae and Cucurbitaceae families. The unique structural features of the cyclotides make them extremely resistant to chemical, thermal and proteolytic degradation. In this article we review recent Studies from our laboratory that dissect the role of the individual structural elements in defining the stability of cyclotides. The resistance of cyclotides to chemical and proteolytic degradation is in large part due to the cystine knot, whereas the thermal stability is I composite of several features including the cystine knot, the cyclic backbone and the hydrogen bonding network. A range of biological activities of cyclotides is critically dependent oil the presence of the cyclic backbone.

A DESMUSICALIZAÇÃO DA MÍDIA EM TEMPOS DE HIPERMIDIATIZAÇÃO DA MÚSICA: o empobrecimento estético da música popular na programação televisiva brasileira

O trabalho aborda o empobrecimento estético-discursivo da canção popular massiva exposta na mídia televisiva brasileira, na comparação entre o contexto histórico-midiático-musical das décadas de 1960 e 1970 e o contexto da primeira metade da segunda década do século XXI. Para definir tal empobrecimento discursivo encontrou-se o conceito de desmusicalização da mídia televisiva . O trabalho, de natureza teórica, tem como objetivo apontar que a mídia televisiva tem papel preponderante e parte importante da responsabilidade pelo empobrecimento na construção do discurso da música popular brasileira. Para tanto, primeiramente, por meio da análise poético-estética dos elementos estruturais das linguagens musical e literária de uma canção popular, delimitou-se a espécie de obra musical estruturada com acuro poético, que a ela empresta um padrão estético de excelência. Tais canções tinham espaços de veiculação nas grades de programação da mídia televisiva de outrora e nos dias atuais não mais os têm. Em seguida, por meio de pesquisas bibliográfica, documental e empírica, descreve-se de modo analítico-interpretativo o período histórico em que ocorreu essa desmusicalização da mídia. Por fim, constata-se que a partir da popularização da Internet e da proliferação de diversas mídias que permitem ao ouvinte o acesso, o armazenamento, o compartilhamento de repertórios musicais e, sobretudo, a audição em movimento, a canção popular massiva pautada pelo acuro poético deixou de estar presente na mídia televisiva e passou a figurar em outras diversas mídias. Essa ampla presença da Música nas mídias digitais definiu-se pelo conceito de hipermidiatização da música, que seria um dos componentes da desmusicalização da mídia televisiva.

PRÁTICAS RESTAURATIVAS: ALTERNATIVA DE MEDIAÇÃO DE CONFLITOS NA ESCOLA UMA OPÇÃO PELA HUMANIZAÇÃO DAS RELAÇÕES

Esta pesquisa teve por objetivo apresentar na forma de relato de experiência, a mediação de conflitos por meio das práticas restaurativas no contexto escolar. Dessa forma pretendeu-se compartilhar um processo ocorrido em uma escola pública de um munícipio do Estado de São Paulo, que utilizou as práticas restaurativas na mediação de conflitos com alunos do 1º ano do ciclo I. Trata-se de uma pesquisa que utilizou o relato de experiência, como forma de construir conhecimentos que pudessem ser socializados e que implicaram em uma modificação da prática. A análise foi feito por meio de através de registros reflexivos, análise documental e observações. No processo de mediação de conflitos, foram utilizadas as práticas restaurativas, sendo o instrumento os círculos restaurativos que visam à resolução do conflito e a prevenção da violência através do diálogo, da reflexão e de um acordo entre as partes. Esta pesquisa mostrou que os círculos propiciaram a reflexão das crianças sobre suas ações, o diálogo entre todos os segmentos envolvidos, o fortalecimento de valores morais, o estímulo à utilização da mediação para a resolução de situações de conflito de forma assertiva e a construção de um ambiente cooperativo que atuou na direção da prevenção de ações violentas. Os princípios da Justiça Restaurativa favoreceram o desenvolvimento da autonomia moral, a cooperação no ambiente escolar e a restauração das relações.

EXPERIÊNCIAS COM CÍRCULOS RESTAURATIVOS EM UMA ESCOLA PÚBLICA NO ESTADO DO AMAZONAS

Este estudo aborda o problema da violência na escola. A escolha do tema deveu-se ao fato de a violência nas escolas públicas brasileiras ter sido assunto diário na mídia nos últimos anos, com consequências devastadoras que afetam a formação da cidadania, principalmente quando crianças e adolescentes são envolvidos. Utilizou-se o método de pesquisa qualitativa por esperar compreender e descrever as experiências dos círculos restaurativos cujos objetivos são: 1) Avaliar a percepção de segurança de alunos e servidores de uma escola pública do estado do Amazonas e conhecer os tipos de violência com as quais se confrontam; 2) identificar e descrever o perfil socioeconômico das famílias dos participantes dos círculos restaurativos; e 3) descrever as experiências com os círculos restaurativos. Participaram da pesquisa 300 indivíduos de uma escola pública do Estado do Amazonas, distribuídos em 248 alunos, 49 servidores, sendo 148 do sexo masculino (49 por cento e 152 (51 por cento do sexo feminino, com idade média de (18,82) anos. Destes 210 (70 por cento ) solteiros, 33 (11 por cento ) casados, e mais 57 (19 por cento ) com outras situações conjugais. A escolaridade variava entre ensino fundamental incompleto (4 por cento ), ensino médio incompleto (83,9 por cento ) e mestrado completo (4 por cento ). Para coleta dos dados, utilizou-se um questionário com 11 itens, a escala sobre violência na escola com 8 itens e as técnicas dos círculos restaurativos. Trata-se, portanto, de uma pesquisa participante por ter utilizado, para coleta dados, as técnicas de observação participante e entrevista semiestruturada. Quanto aos resultados, observou-se uma correlação moderada e significativa entre as variáveis, percepção de segurança na sala de aula com a da escola (r=0,612; p<0,01). Conclui-se que existe evidência de violência nos diversos contextos estudados, sendo que a ocorrência de violência informada é maior nos arredores da escola: 127 (46,4 por cento ). Dos participantes, 160 (58 por cento ) sentem-se seguros. Entretanto, um percentual considerável, 94 (34,3 por cento ) responderam que não se sentem muito seguros. Quanto ao perfil socioeconômico, detectaram-se inúmeros problemas de natureza socioeconômicos e psicossociais, dentre os quais, os conflitos familiares, pobreza, ausência dos serviços públicos básicos ou dificuldades no acesso, o desemprego, analfabetismo, negligência contra crianças e adolescentes entre outros. Em relação às experiências com os círculos restaurativos, foram mediados quatro conflitos e instaurados os pré-círculos e os círculos: dois em decorrência do bullying praticado por meio de mensagens de celular, uma desavença entre dois alunos e um desentendimento entre professor e aluno. Todos os casos tiveram resultados satisfatórios.

Neurite outgrowth by the alternatively spliced region of human tenascin-C is mediated by neuronal alpha7beta1 integrin

The region of tenascin-C containing only alternately spliced fibronectin type-III repeat D (fnD) increases neurite outgrowth by itself and also as part of tenascin-C. We previously localized the active site within fnD to an eight amino acid sequence unique to tenascin-C, VFDNFVLK, and showed that the amino acids FD and FV are required for activity. The purpose of this study was to identify the neuronal receptor that interacts with VFDNFVLK and to investigate the hypothesis that FD and FV are important for receptor binding. Function-blocking antibodies against both alpha7 and beta1 integrin subunits were found to abolish VFDNFVLK-mediated process extension from cerebellar granule neurons. VFDNFVLK but not its mutant, VSPNGSLK, induced clustering of neuronal beta1 integrin immunoreactivity. This strongly implicates FD and FV as important structural elements for receptor activation. Moreover, biochemical experiments revealed an association of the alpha7beta1 integrin with tenascin-C peptides containing the VFDNFVLK sequence but not with peptides with alterations in FD and/or FV. These findings are the first to provide evidence that the alpha7beta1 integrin mediates a response to tenascin-C and the first to demonstrate a functional role for the alpha7beta1 integrin receptor in CNS neurons.

A study of the electrical and optical properties of unhydrogenated neon-sputtered amorphous silicon

Films of amorphous silicon (a-Si) were prepared by r.f. sputtering in a Ne plasma without the addition of hydrogen or a halogen. The d.c. dark electrical conductivity, he optical gap and the photoconductivity of the films were investigated for a range of preparation conditions, the sputtering gas pressure, P, the target-substrate spacing, d, the self-bias voltage, Vsb, on the target and the substrate temperature, Ts. The dependence of the electrical and optical properties on these conditions showed that various combinations of P, d and Vsb, at a constant Ts, giving the same product (Pd/V sb) result in films with similar properties, provided that P, d and Vsb remain vithin a certain range. Variation of Pd/Vsb between about 0.2 and 0.8 rrTorr.cm!V varied the dark conductivity over about 4 orders of magnitude, the optical gap by 0.5 eV and the photoconductivity over 4-5 orders of magnitude. This is attributed to controlling the density-of-states distribution in the mobility gap. The temperature-dependence of photoconductivity and the photoresponse of undoped films are in support of this conclusion. Films prepared at relatively high (Pd/Vsb) values and Ts=300 ºc: exhibited low dark-conductivity and high thermal activation energy, optical gap and photoresponse, characteristic properties of a 'low density-of-states material. P-type doping with group-Ill elements (Al, B and Ga) by sputtering from a composite target or from a predoped target (B-.doped) was investigated. The systematic variation of room-temperature conductivity over many orders of magnitude and a Fermi-level shift of about 0.7 eV towards the valence-band edge suggest that substitutional doping had taken place. The effects of preparation conditions on doping efficiency were also investigated. The post-deposition annealing of undoped and doped films were studied for a temperature range from 250 ºC to 470 ºC. It was shown that annealing enhanced the doping efficiency considerably, although it had little effect on the basic material (a-Si) prepared at the optimum conditions (Pd/Vsb=0.8 mTorr.cm/V and Ts=300 $ºC). Preliminary experiments on devices imply potential applications of the present material, such as p-n and MS junctions.

Identification and analysis of moderator variables:investigating the customer satisfaction-loyalty link

Purpose – Research on the relationship between customer satisfaction and customer loyalty has advanced to a stage that requires a more thorough examination of moderator variables. Limited research shows how moderators influence the relationship between customer satisfaction and customer loyalty in a service context; this article aims to present empirical evidence of the conditions in which the satisfaction-loyalty relationship becomes stronger or weaker. Design/methodology/approach – Using a sample of more than 700 customers of DIY retailers and multi-group structural equation modelling, the authors examine moderating effects of several firm-related variables, variables that result from firm/employee-customer interactions and individual-level variables (i.e. loyalty cards, critical incidents, customer age, gender, income, expertise). Findings – The empirical results suggest that not all of the moderators considered influence the satisfaction-loyalty link. Specifically, critical incidents and income are important moderators of the relationship between customer satisfaction and customer loyalty. Practical implications – Several of the moderator variables considered in this study are manageable variables. Originality/value – This study should prove valuable to academic researchers as well as service and retailing managers. It systematically analyses the moderating effect of firm-related and individual-level variables on the relationship between customer satisfaction and loyalty. It shows the differential effect of different types of moderator variables on the satisfaction-loyalty link.

Multialgebraic Systems in Information Granulation

In different fields a conception of granules is applied both as a group of elements defined by internal properties and as something inseparable whole reflecting external properties. Granular computing may be interpreted in terms of abstraction, generalization, clustering, levels of abstraction, levels of detail, and so on. We have proposed to use multialgebraic systems as a mathematical tool for synthesis and analysis of granules and granule structures. The theorem of necessary and sufficient conditions for multialgebraic systems existence has been proved.

Some MDS Codes over Gf(64) Connected with the Binary Doubly-Even [72,36,16] Code

* The author is supported by a Return Fellowship from the Alexander von Humboldt Foundation.

The Nonexistence of some Griesmer Arcs in PG(4, 5)

In this paper, we prove the nonexistence of arcs with parameters (232, 48) and (233, 48) in PG(4,5). This rules out the existence of linear codes with parameters [232,5,184] and [233,5,185] over the field with five elements and improves two instances in the recent tables by Maruta, Shinohara and Kikui of optimal codes of dimension 5 over F5.

Analysis and design of tall concrete buildings : an investigation regarding the use of cracked versus un-cracked moment of inertia

A debate is currently prevalent among the structural engineers regarding the use of cracked versus un-cracked moment of inertia of the structural elements in analyzing and designing tall concrete buildings. (The basic definition of a tall building, according to the Journal of Structural Design of Tall Buildings Vol. 13. No. 5, 2004 is a structure that is equal to or greater than 160 feet in height, or 6 stories or greater.) The controversy is the result of differing interpretations of certain ACI (American Concrete Institute) code provisions. The issue is whether designers should use cracked moment of inertia in order to estimate lateral deflection and whether the computed lateral deflection should be used to carry out subsequent second-order analysis (analysis considering the effect of first order lateral deflections on bending moment and shear stresses). On one hand, bending moments and shear forces estimated based on un-cracked moment of inertia of the sections may result in conservative designs by overestimating moments and shears. On the other hand, lateral deflections may be underestimated due to the same analyses resulting in unsafe designs.

A gestão do programa saúde na escola no município de Natal/RN: um estudo de caso

The current conception of health deals with several influential factors, having education among them. Intersectoral organization is essential for Young and adult healthcare services. In this context the Healthcare in School Program was created which foresees a continuous articulation between health and education aiding the effectuation of the Healthcare Single System. The objective of this research is analyze the Healthcare in School Program (HSP) in Natal city in Rio Grande do Norte State taking into consideration the Intersectoriality of actions from the standpoint of the management. The chosen method was the case study, with qualitative approach. The sample was of the intentional kind including all components of the Natal city Intersectorial work group, composed by representatives of the Municipal Education Bureau, the State Education Bureau and Healthcare Municipal Bureau. The collecting data technique was the semi-structured interview. The data analysis was performed through the analysis of contents technique. For Data Show the following analysis categories were considered: Meaning of Intersectoriality; Actions Planning; Permanent and ongoing training for autonomy regarding to Healthcare Promotion; Difficulties and Potentials for actions operationalization. The outcomes allow us to indentify in Natal HSP intersectoral practices not developed yet. The manager professionals of Healthcare and Education do not get to acknowledge the power of Intersectoriality yet. The lack of commitment of some professionals stands out, planning is performed in a sectorial basis and without active participation of learners and community, there is duties accumulation and discouragement group, structural inadequacy and difficulty on the ongoing of the program actions. Despite the existing fragmentation, the program has contributed to the professional qualification and development of education actions regarding to healthcare along with learners. Therefore we conclude that healthcare, education and society have lots of challenges to face in order to consolidate Intersectoriality and the Healthcare in School Program and the and the implementation of the guidelines of the Healthcare Single System in Natal city in the state of Rio Grande do Norte.

Controle ativo de vibração em estruturas inteligentes utilizando um controlador por modos deslizantes com compensação difusa

Smart structures and systems have the main purpose to mimic living organisms, which are essentially characterized by an autoregulatory behavior. Therefore, this kind of structure has adaptive characteristics with stimulus-response mechanisms. The term adaptive structure has been used to identify structural systems that are capable of changing their geometry or physical properties with the purpose of performing a specific task. In this work, a sliding mode controller with fuzzy inference is applied for active vibration control in an SMA two-bar truss. In order to obtain a simpler controller, a polynomial model is used in the control law, while a more sophisticated version, which presents close agreement with experimental data, is applied to describe the SMA behavior of the structural elements. This system has a rich dynamic response and can easily reach a chaotic behavior even at moderate loads and frequencies. Therefore, this approach has the advantage of not only obtaining a simpler control law, but also allows its robustness be evidenced. Numerical simulations are carried out in order to demonstrate the control system performance.

Controle ativo de vibração em estruturas inteligentes utilizando um controlador por modos deslizantes com compensação difusa

Smart structures and systems have the main purpose to mimic living organisms, which are essentially characterized by an autoregulatory behavior. Therefore, this kind of structure has adaptive characteristics with stimulus-response mechanisms. The term adaptive structure has been used to identify structural systems that are capable of changing their geometry or physical properties with the purpose of performing a specific task. In this work, a sliding mode controller with fuzzy inference is applied for active vibration control in an SMA two-bar truss. In order to obtain a simpler controller, a polynomial model is used in the control law, while a more sophisticated version, which presents close agreement with experimental data, is applied to describe the SMA behavior of the structural elements. This system has a rich dynamic response and can easily reach a chaotic behavior even at moderate loads and frequencies. Therefore, this approach has the advantage of not only obtaining a simpler control law, but also allows its robustness be evidenced. Numerical simulations are carried out in order to demonstrate the control system performance.

O centro de Fortaleza como lugar de moradia

The present study has the objective of analyzing how the ways of dwelling influences the uses and the functional and symbolic appropriations of the urban space in Fortaleza city center, through the perspective of the residents and their relation with the living place. Scenarios of development and degradation of its urban space have marked the city center of Fortaleza. The intensification of the commercial and services occupation promoted through the years, paradoxically, the valorization of the land price and the devalorization of the residential use. Thus, the residential occupation was consolidated in a discontinuous way, being concentrated mainly in the external limits of the historical core of the neighborhood. The research is structured over the delimitation of an area and the selection of multifamily residential buildings, built since the 1960s, close to the central core of the neighborhood. The spatial configuration analysis of the selected residential buildings, and their relations with the urban surroundings and the land uses, reveals different aspects related to the urban vitality, producing impacts over the ways of dwelling constituted by the daily life of the residents. The study of the ways of dwelling involves the comprehension that the dwelling is beyond the private residential boundaries and the functional occupation. The conceptual base of this research is developed over the perspective that the dwelling represents a fundamental aspect of the human condition, allowing the person to relate with the space in an essential way (HEIDEGGER, 2012). In this perspective of the dwelling, the space reunites the mental and the cultural, the social and the historical, being marked by simultaneous logics of the conceived, the perceived and the lived space (LEFEVBRE, 2006). The development of this study, over the point of view of the neighborhood residents, is inserted in the perspective of the lived space, related to the concept of place, understood as a qualitative phenomenon, giving essence and identity to the space. The place of dwelling, marked by coexistences, is one of the structural elements of the urban land use, and potential for the rehabilitation of the central areas in big cities. Therefore, the study starts from the hypothesis that the location, the nearby land uses and the spatial configuration of the residential buildings affect the ways of dwelling, in other words the residents dissolve the boundaries of the shelter and experience the urban space, from the dwelling place. The requalification of the dwelling is opposed to the residential space based in functionality, in hierarchy, in self sufficiency, in standardization and in the reproduction of the urban space, materialized in the dense contemporary residential buildings, that are unrelated to the city. The stages of the research involved the spatial configuration analysis of the selected residential buildings and their surrounding, the land use mapping and interviews with the residents. The collected data allowed verifying that the buildings are located in areas presenting heterogeneous land uses, with a great number of residents and users. However, these aspects are not sufficient to promote the vitality of the public spaces in the neighborhood, since the people movement in the streets is controlled by the opening hours of the predominant commercial use in the area. The word of the residents, collected in interviews, indicate that the conservation conditions of the public spaces and the insecurity influences their everyday relations with the place of dwelling, affecting fundamental aspects for the dwelling requalification in the central area of Fortaleza.