The role of DT-diaphorase in the maintenance of the reduced antioxidant form of coenzyme Q in membrane systems.

The experiments reported here were designed to test the hypothesis that the two-electron quinone reductase DT-diaphorase [NAD(P)H:(quinone-acceptor) oxidoreductase, EC 1.6.99.2] functions to maintain membrane-bound coenzyme Q (CoQ) in its reduced antioxidant state, thereby providing protection from free radical damage. DT-diaphorase was isolated and purified from rat liver cytosol, and its ability to reduce several CoQ homologs incorporated into large unilamellar vesicles was demonstrated. Addition of NADH and DT-diaphorase to either large unilamellar or multilamellar vesicles containing homologs of CoQ, including CoQ9 and CoQ10, resulted in the essentially complete reduction of the CoQ. The ability of DT-diaphorase to maintain the reduced state of CoQ and protect membrane components from free radical damage as lipid peroxidation was tested by incorporating either reduced CoQ9 or CoQ10 and the lipophylic azoinitiator 2,2'-azobis(2,4-dimethylvaleronitrile) into multilamellar vesicles in the presence of NADH and DT-diaphorase. The presence of DT-diaphorase prevented the oxidation of reduced CoQ and inhibited lipid peroxidation. The interaction between DT-diaphorase and CoQ was also demonstrated in an isolated rat liver hepatocyte system. Incubation with adriamycin resulted in mitochondrial membrane damage as measured by membrane potential and the release of hydrogen peroxide. Incorporation of CoQ10 provided protection from adriamycin-induced mitochondrial membrane damage. The incorporation of dicoumarol, a potent inhibitor of DT-diaphorase, interfered with the protection provided by CoQ. The results of these experiments provide support for the hypothesis that DT-diaphorase functions as an antioxidant in both artificial membrane and natural membrane systems by acting as a two-electron CoQ reductase that forms and maintains the antioxidant form of CoQ. The suggestion is offered that DT-diaphorase was selected during evolution to perform this role and that its conversion of xenobiotics and other synthetic molecules is secondary and coincidental.

Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences.

RNA editing by adenosine deamination in brain-expressed pre-mRNAs for glutamate receptor (GluR) subunits alters gene-specified codons for functionally critical positions, such as the channel's Q/R site. We show by transcript analysis of minigenes transiently expressed in PC-12 cells that, in contrast to GluR-B pre-mRNA, where the two editing sites (Q/R and R/G) require base pairing with nearby intronic editing site complementary sequences (ECSs), editing in GluR5 and GluR6 pre-mRNAs recruits an ECS located as far as 1900 nucleotides distal to the Q/R site. The exon-intron duplex structure of the GluR5 and GluR6 pre-mRNAs appears to be a substrate of double-stranded RNA-specific adenosine deaminase. This enzyme when coexpressed in HEK 293 cells preferentially targets the adenosine of the Q/R site and of an unpaired position in the ECS which is highly edited in brain.

Coenzyme Q reductase from liver plasma membrane: purification and role in trans-plasma-membrane electron transport.

A specific requirement for coenzyme Q in the maintenance of trans-plasma-membrane redox activity is demonstrated. Extraction of coenzyme Q from membranes resulted in inhibition of NADH-ascorbate free radical reductase (trans electron transport), and addition of coenzyme Q10 restored the activity. NADH-cytochrome c oxidoreductase (cis electron transport) did not respond to the coenzyme Q status. Quinone analogs inhibited trans-plasma-membrane redox activity, and the inhibition was reversed by coenzyme Q. A 34-kDa coenzyme Q reductase (p34) has been purified from pig-liver plasma membranes. The isolated enzyme was sensitive to quinone-site inhibitors. p34 catalyzed the NADH-dependent reduction of coenzyme Q10 after reconstitution in phospholipid liposomes. When plasma membranes were supplemented with extra p34, NADH-ascorbate free radical reductase was activated but NADH-cytochrome c oxidoreductase was not. These results support the involvement of p34 as a source of electrons for the trans-plasma-membrane redox system oxidizing NADH and support coenzyme Q as an intermediate electron carrier between NADH and the external acceptor ascorbate free radical.

Calidad y rentabilidad: análisis del certificado Q en las cadenas hoteleras

La implantación de sistemas de calidad aporta a las empresas beneficios internos y externos. Este artículo analiza la relación entre la certificación de calidad del Instituto para la Calidad Turística Española y los resultados y el tamaño de las cadenas hoteleras con presencia en España. Los análisis muestran que la certificación tiene efectos positivos en los resultados y que el tamaño de la cadena no es un factor importante para certificarse.

Weighted composition operators on the predual and dual Banach spaces of Beurling algebras on Z

Let vv be a weight sequence on ZZ and let ψ,φψ,φ be complex-valued functions on ZZ such that φ(Z)⊂Zφ(Z)⊂Z. In this paper we study the boundedness, compactness and weak compactness of weighted composition operators Cψ,φCψ,φ on predual Banach spaces c0(Z,1/v)c0(Z,1/v) and dual Banach spaces ℓ∞(Z,1/v)ℓ∞(Z,1/v) of Beurling algebras ℓ1(Z,v)ℓ1(Z,v).

Nile River and Birkat Qārūn Regions, Egypt, 1854 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Carte hydrographique de la Moyenne Égypte, où sont indiqués les travaux exécutés ou à exécuter, d'après les ordres de Son Altesse Mehémet-Ali, Vice-Roi d'Egypte. Avec le projet de communication directe des deux mers au travers de l'Isthme, par M. Linant de Bellefonds ; gravée par Schwaerzlé et Erhard. It was published by Dépôt de la Guerre in 1854. Scale 1:250,000. Covers a portion of the Nile River region near Birkat Qārūn, Banī Suwayf, Al Fayyūm, and Al Minyā. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Egypt Red Belt projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, canals, roads, cities and other human settlements, administrative boundaries, ruins and historical sites, and more. Relief shown by hachures. Includes also text and notes.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Hādhihi Taqrīrāt rāʼiqah wa-taḥqīqāt fāʼiqah

nammaqahā Muḥammad al-Azharī najl al-Ḥasan al-ʻAdawī al-Ḥamzāwī. Washshaḥa bihā ḥawāshī Muḥammad al-Ḥifnī ʻalá Sharḥ al-Risālah al-waḍʻīyah al-ʻAḍudīyah / li-Abī al-Qāsim al-Samarqandī wa-bi-hāmishihā al-ḥawāshī al-madhkūrah maʻa al-sharḥ al-madhkūr.

Talfīq al-akhbār wa-talqīḥ al-āthār fī waqāʻiʻ Qāzān wa-Bulghār wa-mulūk al-Tatār

athar M. M. al-Ramzī.

Kitāb Sullam al-ʻulūm wa-ḥāshiyatihi al-mashhūrah bi-al-Qāḍī maʻa minhīyātih

[Muḥibb Allāh ibn ʻAbd al-Shakūr al-Bihārī].

Qānūn dīwān al-rasāʼil: manqūl ʻan nuskhah khaṭṭīyah bi-Maktabat Kambritsh

li-Tāj al-Riyāsah Abī al-Qāsim ʻAlī ibn Munjib ibn Sulaymān al-shahīr bi-Ibn al-Ṣayrafī ; ʻuniya bi-nashrihi wa-al-taʻlīq ʻalayhi ʻAlī Bahjat.

al- Khulāṣah al-naqīyah fī umarāʼ Ifrīqīyah: wa-maʻahu ʻUqūd al-farāʼid fī tadhyīl al-Khulāṣah wa-fawāʼid al-rāʼid lil-muʼallif ayḍan

taʼlīf Abī ʻAbd Allāh Muḥammad al-Bājī al-Masʻūdī.

Nāmūs al-īqān

Kharbūtī Yūsuf Afandī.

Kitāb Qāmūs al-ʻāshiqīn fī akhbār al-Sayyid Ḥusayn Burhān al-Dīn

taʼlīf ʻAbd al-Munʻim al-ʻĀnī thumma al-Rāwī ; [muṣaḥḥiḥuhu Aḥmad ibn Saʻd al-Dīn ibn Muṣṭafá al-Qabbānī].

Hādhihi Tarjamat quṭb al-wāṣilīn wa-ghawth al-sālikīn al-ʻārif billāh Sayyidī Shams al-Dīn Muḥammad Abī al-Maḥāsin al-Qāwuqjī al-Ḥasanī

min qalam ʻAbd al-Qādir al-Adʹhamī al-Ṭarābulusī.

Kitāb Tuḥfat ahl al-futūḥāt wa-al-adhwāq fī ittikhādh al-subḥah wa-jaʻlihā fī al-aʻnāq wa-baʻḍ al-ādāb al-lāʼiqah bi-al-mukarramīn bi-ṣuḥbat ahl ḥaḍrat al-iṭlāq bi-faḍl al-karīm al-khallāq

lil-Shaykh Fatḥ Allāh ibn al-Shaykh Sayyidī Abī Bakr al-Bannānī.