Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas

Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil. The cyclodextrins (CDs) production by CGTases was evaluated using starches from different sources. CDs were measured through the Complexation Theory and by HPLC. CGTase from Bacillus firmus strain 7B showed the best production (30 mmol/L of β-CD and 4.3 mmol/L of γ-CD), and its cultivation conditions were optimized. The maximum enzymatic activity was achieved using lung peptone, soluble starch and agitation speed of 160 rpm. Studied CGTases were shown quite interesting for the industrial production of CDs.

Designer drugs: aspectos analíticos e biológicos

In the recent years, analytical toxicologists have been facing difficulties in detecting designer drugs due to the chemical modifications on the existing structures and the speed in which they are released into the market, requiring the development and improvement of specific and appropriate analytical methods. This work is a review of the literature which summarizes the characteristics of the drugs and the analytical validated methods using conventional and unconventional matrices currently used for correct identification and quantification of the following classes of emerging drugs of abuse: derivatives of opiates, amphetamines, tryptamines, piperazines and cannabinoids.

Avaliação do processo adsortivo utilizando mesocarpo de coco verde para remoção do corante cinza reativo BF-2R

This work used green coconut mesocarp as a bioadsorbent to remove Reactive Gray BF-2R dye. A 2³ factorial design was used to evaluate the influence of the variables adsorbent mass, particle size and stirring speed on the adsorptive process. Kinetic and adsorption equilibrium studies were performed. Results showed that the kinetic equilibrium was reached after 150 min. Using the Langmuir model, a q max of 21.9 mg g-1 and k of 0.30 L g-1 was obtained. The mesocarp of coconut, a residue of agribusiness, proved to be an effective alternative technique for the removal of dye in this study.

Development of a simple analytical method for determining trihalomethanes in beer using a headspace solid-phase microextraction technique

We developed a simple, rapid, and solventless method for analyzing trihalomethanes in beer samples using headspace solid-phase microextraction. The effects of varying experimental parameters, such as extraction temperature and time, addition of sodium chloride, and agitation speed, on extraction yield were studied using a univariate experimental design. Limits of detection between 0.22 and 0.46 µg L- 1 and wide linear ranges were achieved for trihalomethanes. We measured the trihalomethane recoveries and precision (as the standard deviation of repeat measurements) and demonstrated the applicability of the proposed method by analyzing 32 beer samples.

Pequisas físicas e químicas do sistema hidrográfico da região lagunar de Cananéia: I - Cursos de águas

The present elaboration is a "previous note" about two watercourses, Maria Rodrigues and Baguaçú, belonging to Hydrografic System of the Cananeas Lagoon Region in the Southern Part of it he Coast of the Estado de São Paulo, Brazil, which is the objete of our physical and chemical researches. It is based on the first results obtained from our contact with that region, ttfie studies of which are being continued and will still have to confirm or not the previous conclusions, which we are presenting in this "provisional paper". We studied the Maria Rodrigues and the Baguacu under the discussion whether they are to be clasified as a river in the poper sense ot the word or waUer-courses of any other origin. For this purpose we established observatory-stations along their whole course where we collected quantities of water to be analised. The data included in the table nº 1 and 2 refer to the physical and chemical properties of these portions of water. These physical and chemical properties were discussed and compared one with the other according to the observatory-stations, at the same moment and the tide predominating on that occasion. The physical and chemical datas were obtained according to the norms recommended by the Conseil International permanent pour l'Exploration de la Mer, Copenhague. We also presented a sketch of both; we described their relations with regard to the Mar de Cananéia; we inserted comprehensible histograms of the distribution of the observatory-stations, each of them with the respective indices, and at last we tried to correlate the occurrence of the "plancton" with the physical and chemical properties of water as well in the Maria Rodrigues as in the Baguacú. From these researches result the following previous conclusions: 1 - By the data obtained up to now nothing could be formulated as to a cyclical or "seasonal" aspect. 2 - By the comparison of the phenomena which present the rivers in the proper sense of the word and those observed in the Maria Rodrigues and the Baguacú, we are convinced that both are water-courses of the classical type "marigot,". 3 - Their channels are maintained open by the effect of the tide and, more perphaps, by the infiltration-water deriving from the underground-water level owing to the type of the soil of that region, which in mean shows an elevation of 2-4 meters over sea-level. 4 - From tables 1 and 2 and histograms 2 and 3 we stated that the water, not only of the Maria Rodrigues but also of the Baguacú, conserve more or less the same physical and chemical properties, provided that: 1) the localities to be compared are the mouths or equidistant points from them in the lower course, and 2) the moment and tide must be the same. Tables 3a and 3b show sufficiently this supposition.

Pumppukäyttöjen energiankulutuksen määrittäminen prosessimittauksia käyttäen

Pumppukäytöt vastaavat noin neljännestä Euroopan alueen sähkömoottoreissa kuluvasta energiasta. Energian hinnan nousun vuoksi energian säästäminen ja energiatehokkuus ovat nousseet tärkeään asemaan paljon energiaa kuluttavassa teollisuudessa. Pumppukäyttöjen hyötysuhteen parantaminen on noussut olennaiseen osaan paperi- ja kartonkiteollisuuden energiatehokkuustarkasteluissa. Tässä työssä tarkastellaan kartonkikoneen pumppukäyttöjen toiminnan energiatehokkuutta moottorin virtamittausten perusteella. Analyysi perustuu moottorin akselitehon määrittämiseen ja sen perusteella tehtävään pumpun toimintapisteen laskentaan. Työssä esitellään käytetyt estimointimenetelmät ja niillä saadut tulokset kartonkikoneen pumppukäytöille. Lisäksi työssä arvioidaan kolmen yksittäisen pumppukäytön energiankulutuksen säästöpotentiaalia. Työssä käytettyä menetelmää voidaan käyttää sekä vakio- että vaihtonopeuspumppukäyttöjen toiminnan ja hyötysuhteen analysointiin.

Ruokohelven käytön lisääminen Joensuun voimalaitoksen kattilassa – käytettävyys, hyötysuhde ja taloudellisuus

Ruokohelpi soveltuu ympäristöystävällisyyden ja korkean lämpöarvon vuoksi hyvin energiantuotantoon. Fortumin Joensuun voimalaitoksella ruokohelpi on syötetty kattilaan tähän asti pääasiassa pääpolttoaineisiin, turpeeseen tai hakkeeseen seostettuna pieninä energiaosuuksina. Pääpolttoaineisiin verrattuna ruokohelvellä on alhaisempi irto- ja energiatiheys, korkeampi klooripitoisuus ja kattilaa likaavampi tuhka, mikä asettaa rajoitteita sen käytön lisäämiselle voimalaitoksella. Alhaisesta irto- ja energiatiheydestä johtuvan holvautumisen sekä tukoksien lisääntymisen ja Joensuun voimalaitoksen nykyisten kuljettimien kapasiteettiongelmien vuoksi ruokohelven osuuden lisääminen suuremmaksi kuin 5 % polttoaine-energiasisällöstä on riskialtista ja edellyttää täten investointia erilliseen ruokohelven käsittely- ja syöttöjärjestelmään. Yksi vaihtoehto on murskata ruokohelpipaalit joko sähkökäyttöisellä, puolikiinteällä ja nopeakäyntisellä Haybuster H1130 tilt -murskaimella tai kiinteällä ja hidaskäyntisellä Raumaster-murskaimella ja ohjata ruokohelpisilppu joko pitkän mekaanisen kuljettimen ja sen perässä olevien lyhyiden pneumalinjojen tai pelkkien pitkien pneumalinjojen kautta suoraan kattilapesään. Työssä tutkitut investoinnit ovat taloudellisesti sitä kannattavampia mitä enemmän ruokohelpeä voidaan vuositasolla polttaa voimalaitoksella. Ruokohelven käyttömäärää voimalaitoksella kannattanee lisätä kuljetusmatkaa pidentämällä. Investointien valinta ei ole itsestäänselvyys. Nopeakäyntinen murskain on hidaskäyntistä murskainta edullisempi investointi, tosin hidaskäyntisen murskaimen käyttövarmuus on parempi kuin nopeakäyntisen murskaimen. Kattilan käytettävyyden kannalta ruokohelven käytön lisääminen edellyttää kattilan palamistekniikan analysointia laskennallisesti virtausmallinnuksella ennen kuin lopullisia päätöksiä investointien suhteen voidaan tehdä.

Broadband excitation in the system identification of active magnetic bearing rotor systems

One of the targets of the climate and energy package of the European Union is to increase the energy efficiency in order to achieve a 20 percent reduction in primary energy use compared with the projected level by 2020. The energy efficiency can be improved for example by increasing the rotational speed of large electrical drives, because this enables the elimination of gearboxes leading to a compact design with lower losses. The rotational speeds of traditional bearings, such as roller bearings, are limited by mechanical friction. Active magnetic bearings (AMBs), on the other hand, allow very high rotational speeds. Consequently, their use in large medium- and high-speed machines has rapidly increased. An active magnetic bearing rotor system is an inherently unstable, nonlinear multiple-input, multiple-output system. Model-based controller design of AMBs requires an accurate system model. Finite element modeling (FEM) together with the experimental modal analysis provides a very accurate model for the rotor, and a linearized model of the magneticactuators has proven to work well in normal conditions. However, the overall system may suffer from unmodeled dynamics, such as dynamics of foundation or shrink fits. This dynamics can be modeled by system identification. System identification can also be used for on-line diagnostics. In this study, broadband excitation signals are adopted to the identification of an active magnetic bearing rotor system. The broadband excitation enables faster frequency response function measurements when compared with the widely used stepped sine and swept sine excitations. Different broadband excitations are reviewed, and the random phase multisine excitation is chosen for further study. The measurement times using the multisine excitation and the stepped sine excitation are compared. An excitation signal design with an analysis of the harmonics produced by the nonlinear system is presented. The suitability of different frequency response function estimators for an AMB rotor system are also compared. Additionally, analytical modeling of an AMB rotor system, obtaining a parametric model from the nonparametric frequency response functions, and model updating are discussed in brief, as they are key elements in the modeling for a control design. Theoretical methods are tested with a laboratory test rig. The results conclude that an appropriately designed random phase multisine excitation is suitable for the identification of AMB rotor systems.

JUVENILE-MATURE GENETIC CORRELATIONS IN Pinus taeda CLONES PROPAGATED VIA SOMATIC EMBRYOGENESIS

ABSTRACT This study aimed to estimate the genetic correlation among selection ages (juvenile - adult) and efficiency of early selection for the height, diameter, and volume traits of individuals from Pinus taeda families propagated via somatic embryogenesis. This study was carried out by genetic-statistical analysis, estimation procedure of variance (Reml), and prediction components of breeding values (Blup), using the Selegen-Reml/Blup software. Genetic correlations among juvenile ages and rotation age were performed by applying the linear model developed by Lambeth (1980). In accordance with results of the established model, the early selection can be performed in clones of Pinus taeda with high selection efficiency. Ages from 4 to 6 years old are enough to select Pinus taeda clones propagated via somatic embryogenesis for harvesting at 8 and 12 years old; and 6 to 10 years old are enough to select them for harvesting at 20 years old. On the basis of the genetic correlations estimates from the environments, the clones' selection of Pinus taeda propagated via somatic embryogenesis should be developed specifically for each environment. The clones' selection can be performed considering the diameter due to the high correlation between volume and diameter.

Studies in offset ink setting

The offset printing process is complex and involves the meeting of two essentially complex materials, printing ink and paper, upon which the final product is formed. It can therefore be expected that a multitude of chemical and physical interactions and mechanisms take place at the ink-paper interface. Interactions between ink and paper are of interest to both the papermakers and ink producers, as they wish to achieve better quality in the final product. The objective of this work is to clarify the combined influence of paper coating structure, printing ink and fountain solution on ink setting and the problems related to ink setting. A further aim is to identify the mechanisms that influence ink setting problems, and to be able to counteract them by changing properties of the coating layer or by changing the properties of the ink. The work carried out for this thesis included use of many techniques ranging from standard paper and printability tests to advanced optical techniques for detection of ink filaments during ink levelling. Modern imaging methods were applied for assessment of ink filament remain sizes and distribution of ink components inside pigment coating layers. Gravimetric filtration method and assessment of print rub using Ink-Surface-Interaction-Tester (ISIT) were utilized to study the influence of ink properties on ink setting. The chemical interactions were observed with the help of modified thin layer chromatography and contact angle measurements using both conventional and high speed imaging. The results of the papers in this thesis link the press operational parameters to filament sizes and show the influence of these parameters to filament size distribution. The relative importance between the press operation parameters was shown to vary. The size distribution of filaments is important in predicting the ink setting behaviour, which was highlighted by the dynamic gloss and ink setting studies. Prediction of ink setting behaviour was also further improved by use of separate permeability factors for different ink types in connection to filtration equations. The roles of ink components were studied in connection to ink absorption and mechanism of print rub. Total solids content and ratio of linseed oil to mineral oil were found to determine the degree of print rub on coated papers. Wax addition improved print rub resistance, but would not decrease print rub as much as lowering the total solids content in the ink. Linseed oil was shown to absorb into pigment coating pores by mechanism of adsorption to pore walls, which highlights the need for sufficient pore surface area for improved chromatographic separation of ink components. These results should help press operators, suppliers of printing presses, papermakers and suppliers to papermakers, to better understand the material and operating conditions of the press as it relates to various print quality issues. Even though paper is in competition with electronic media, high quality printed products are still in demand. The results should provide useful information for this segment of the industry.

Adaptation of "Niagara Rosada" grape must to winemaking by partial cluster dehydration

This study aimed to verify the influence of partial dehydration of "Niagara Rosada" grape clusters in physicochemical quality of the pre- fermentation must. In Brazil, during the winemaking process it is common to need to adjust the grape must when the physicochemical characteristics of the raw material are insufficient to produce wines in accordance with the Brazilian legislation for classification of beverages, which establishes the minimum alcohol content of 8.6 % for the beverage to be considered wine. Therefore, given that the reduction in the water content of grape berries allows the concentration of chemical compounds present in its composition, especially the concentration of total soluble solids, we proceeded with the treatments that were formed by the combination of two temperatures (T1-37.1ºC and T2-22.9 ºC) two air speeds (S1: 1.79 m s-1 and S2: 3.21 m s-1) and a control (T0) that has not gone through the dehydration treatment. Analysis of pH, Total Titratable Acidity (TTA) were performed in mEq L-1, Total Soluble Solids (TSS) in ºBrix, water content on a dry basis and Concentration of Phenolic Compounds (CPC) in mg of gallic acid per 100g of must. The average comparison test identified statistically significant modifications for the adaptation of must for winemaking purposes, having the treatment with 22.9 ºC and air speed of 1.79 m s-1 shown the largest increase in the concentration of total soluble solids, followed by the second best result for concentration of phenolic compounds.

Heat loss in one day old pullets inside a hatchery

After hatching, pullets are transported to brooding area and vaccinated. One day old chicks have not already developed thermoregulation ability; thus, brooding temperature variations may affect pullet quality leading to broiler meat production losses. This research aimed to calculate sensible heat loss in one day old pullets in hatching area and vaccination room. Ten one day old pullets were randomly selected from hatching area of a commercial hatchery. Infrared images were used to calculate bird surface temperature. Exposure areas for the two conditions were quantified, and both air temperature and wind speed was recorded. Total sensible heat loss was calculated as heat loss by radiation plus heat loss by convection. It was found that heat transfer occurs in different ways at different bird body parts. Total heat loss found for hatching baskets was equivalent to 0.81 J s-1 while for vaccination room was 1.16 J s-1. Pullet nutrition is based on energy loss from brooding to farm, and the overall pullet heat loss from hatchery to farm accepted is 13.95 J s-1. Thus, "starter feed" has relevant excess of energy input. These findings indicate that less energy can be used in initial feed, once heat loss is lower than assumed nowadays. Improved knowledge on these conditions may enhance broiler farm feeding strategies and economics during first rearing week.

Estimation methods of reference evapotranspiration (ETo) for Uberlândia -MG

One approach to verify the adequacy of estimation methods of reference evapotranspiration is the comparison with the Penman-Monteith method, recommended by the United Nations of Food and Agriculture Organization - FAO, as the standard method for estimating ET0. This study aimed to compare methods for estimating ET0, Makkink (MK), Hargreaves (HG) and Solar Radiation (RS), with Penman-Monteith (PM). For this purpose, we used daily data of global solar radiation, air temperature, relative humidity and wind speed for the year 2010, obtained through the automatic meteorological station, with latitude 18° 91' 66" S, longitude 48° 25' 05" W and altitude of 869m, at the National Institute of Meteorology situated in the Campus of Federal University of Uberlandia - MG, Brazil. Analysis of results for the period were carried out in daily basis, using regression analysis and considering the linear model y = ax, where the dependent variable was the method of Penman-Monteith and the independent, the estimation of ET0 by evaluated methods. Methodology was used to check the influence of standard deviation of daily ET0 in comparison of methods. The evaluation indicated that methods of Solar Radiation and Penman-Monteith cannot be compared, yet the method of Hargreaves indicates the most efficient adjustment to estimate ETo.

Fraud detection in the banking sector : a multi-agent approach

Fraud is an increasing phenomenon as shown in many surveys carried out by leading international consulting companies in the last years. Despite the evolution of electronic payments and hacking techniques there is still a strong human component in fraud schemes. Conflict of interest in particular is the main contributing factor to the success of internal fraud. In such cases anomaly detection tools are not always the best instruments, since the fraud schemes are based on faking documents in a context dominated by lack of controls, and the perpetrators are those ones who should control possible irregularities. In the banking sector audit team experts can count only on their experience, whistle blowing and the reports sent by their inspectors. The Fraud Interactive Decision Expert System (FIDES), which is the core of this research, is a multi-agent system built to support auditors in evaluating suspicious behaviours and to speed up the evaluation process in order to detect or prevent fraud schemes. The system combines Think-map, Delphi method and Attack trees and it has been built around audit team experts and their needs. The output of FIDES is an attack tree, a tree-based diagram to ”systematically categorize the different ways in which a system can be attacked”. Once the attack tree is built, auditors can choose the path they perceive as more suitable and decide whether or not to start the investigation. The system is meant for use in the future to retrieve old cases in order to match them with new ones and find similarities. The retrieving features of the system will be useful to simplify the risk management phase, since similar countermeasures adopted for past cases might be useful for present ones. Even though FIDES has been built with the banking sector in mind, it can be applied in all those organisations, like insurance companies or public organizations, where anti-fraud activity is based on a central anti-fraud unit and a reporting system.

Suurnopeus-turbokoneroottoreiden termodynaaminen ja mekaaninen mallinnus sekä rakenneanalyysi

Tässä väitöskirjassa tarkastellaan suurnopeustekniikan eri sovelluksissa ilmeneviä roottoreihin liittyviä rakenteellisia vaatimuksia ja haasteita. Tässä yhteydessä suurnopeustekniikalla tarkoitetaan järjestelyä, jossa sähkökone (moottori, generaattori) ja toimilaite (turbiini, kompressori, puhallin) on kytketty ilman vaihdetta suoraan mekaanisesti yhteen ja jossa yhteisen roottorin pyörimisnopeus on selvästi suurempi kuin 50/60 hertsin verkosta syötetyn kaksinapaisen vaihtovirtasähkökoneen tahtinopeus. Tyypillistä suurnopeuskoneen roottorille on suuri tehotiheys ja suuri mekaaninen kuormitus. Siksi esimerkiksi sähkökoneen jäähdytys on entistä haasteellisempaa kasvavien rautahäviöiden ja pienempien lämmönsiirtopinta-alojen vuoksi. Tämän työn tavoitteet voidaan jakaa kolmeen osaan:  Yhdistetyn sähkö- ja turbokoneen roottorin mekaanisen rakenteen tarkastelu, jonka tavoitteena on pienentää lämmönkehitystä ja tehostaa kriittisten kohtien jäähdytystä. Tähän liittyy sähkömagneettisten häviöiden keskittäminen jäähdytyksen kannalta edullisiin kohtiin  Yhdistetyn sähkö- ja turbokoneen roottorin mekaanisen rakenteen tarkastelu kriittisten ominaistaajuuksien kannalta  Yhdistetyn sähkö- ja turbokoneen roottorin mekaanisen rakenteen analysointi lujuustekniseltä kannalta. Tähän liittyvät mm. erilaiset ahdistussovitteet ja niiden säilyminen korkeilla pyörimisnopeuksilla sekä niiden roottoria jäykistävä vaikutus ja lämmön johtuminen kyseisissä liitospinnoissa. Tämän työn tieteellinen uutuusarvo on nimenomaan yhdistetyn sähkö- ja turbokoneen roottorin rakenteen analysointi ottamalla samanaikaisesti huomioon kaikki edellä mainitut näkökohdat: jäähtyminen erityisen kuumissa kohdissa, sähköisten häviöiden alentaminen ja niiden jakautuman huomioon ottaminen, roottorin jäykkyyden maksimointi, lujuusrasitusten hallinta ja rakenteen mekaaninen stabiliteetti sekä lämpöteknisten ylimenovastusten tarkastelu.