Detecção de Brucella spp. em leite bovino não pasteurizado através da Reação de Cadeia pela Polimerase (PCR)

Brucellosis is a zoonosis caused by bacteria of the genus Brucella. Man infection occurs through contact with reproductive secretions as placenta and its lochia, semen and penile secretion of infected animals or by consuming unpasteurized milk and dairy products. With the objective of investigating the presence of bacteria in milk, 30 samples of raw milk sold illegally in the region of Botucatu, São Paulo, Brazil, as well as 50 samples of milk delivered to a dairy industry previously to its pasteurization were evaluated by the polymerase chain reaction (PCR) technique. Of the 80 samples analyzed, 10 samples (12.5%) were positive and 70 (87.5%) were negative. Among the  positive samples,  5 (16.6%)  were from  illegal traders  and other  5  (10%) were obtained  from the dairy industry. Brucella spp. positivity shows that the pathogen is representatively present in Botucatu, São Paulo, Brazil, and the risk associated to public health due to the commercialization of illegal products without pasteurization is real.

Ribavirin has an in vitro antiviral effect in rabies virus infected neuronal cells but fails to provide benefit in experimental rabies in mice

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Penectomia em equino com carcinoma de células escamosas

The presence of increased volume in penis and prepuce of horses can be of neoplastic or non-neoplastic origin. Squamous cell carcinoma (SCC), papilloma, fibropapiloma, melanoma and sarcoidorigin. Squamous cell carcinoma (SCC), papilloma, fibropapilloma, melanoma and sarcoid are detached as neoplastic causes. The non-neoplastic causes can be parasitic as habronemiasis and pythiosis, or inflammation, resulting in the formation of exuberant granulation tissue. The penile disorders can be easily confused with each other because they have similar clinical signs. Among epithelial neoplasms affecting penis and prepuce, squamous cell carcinoma is the most common in horses. Thus, it was aimed to report a case of squamous cell carcinoma in a horse and describe the main differential diagnosis for this disease. The additional tests to obtain an accurate diagnosis and the institution of appropriate treatment are essential for the satisfactory repair of penile neoplasms in this species.

Exigências e otimização de isoleucina, valina, triptofano e arginina para matrizes pesadas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Anestesia no transplante de rim de doador vivo no Hospital das Clínicas de Botucatu: fatores de influência para o desfecho

Pós-graduação em Anestesiologia - FMB

Avaliação do potencial citotóxico, genotóxico e mutagênico da melitina em cultura de células

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A Avaliação na sala de aula de matemática: práticas e concepções

This research aims to understand the assessment practices used by teachers at a public state school in the city of Cunha, Sao Paulo. To this end, we interviewed five mathematics teachers, who answered a questionnaire with five questions. The responses were analyzed according to the rigor of phenomenological research. To understand the investigation region, that is to say, the meaning of evaluation, we proceeded to a review of studies on the subject in authors like Buriasco (2002), Pavanello (2006), Hoffmann (1994), expressive in Mathematics Education that allows us to explain the concept of prevailing interpretation in the area. The phenomenological analysis enabled the development of three categories open revealing the concept of evaluation of teachers investigated. The first shows the review As a way to measure the knowledge acquired by the student. His interpretation leads us to understand that for some teachers, the research subjects, the assessment becomes a method to ' measure ' the knowledge acquired by the student. The second category, expressed by As a way of understanding the student's behavior in class, shows that some of the interviewees understand the evaluation as a medium that reveals and appreciates the ways of the student behave in class. Finally, the third category refers to the evaluation by means of said instruments. On this subject the claim that the assessment is through instruments such that: evidence, exercise lists, among others. In summary, interviews and categories analyzed explain the ways in which the assessment reveals the concept of implicit learning the instruments used in the evaluation practices of teachers interviewed. However, the authors read, evaluation is a necessary and permanent teaching job in teaching, which must follow step by step the process of teaching and learning. It follows, ... (Complete abstract click electronic access below)

Benefit Cost Scenarios of Potential Oral Rabies Vaccination for Skunks in California

Scenario-based analyses were computed for benefits and costs linked with hypothetical oral rabies vaccination (ORV) campaigns to contain or eliminate skunk-variant rabies in skunks (Mephitis mephitis) in California, USA. Scenario 1 assumed baiting eight zones (43,388 km2 total) that comprised 73% of known skunk rabies locations in the state. Scenario 2 also assumed baiting these eight zones, but further assumed that added benefits would result from preventing the spread of skunk-variant rabies into Los Angeles County, USA. Scenarios assumed a fixed bait cost ($1.24 each) but varied campaigns (one, two and three annual ORV applications), densities of baits (37.5/km2, 75/km2 and 150/km2), levels of prevention (50%, 75%, and 100%), and contingency expenditures if rabies recurred (20%, 40%, and 60% of campaign costs). Prorating potential annual benefits during a 12-yr time horizon yielded benefit-cost ratios (BCRs) between 0.16 and 2.91 and between 0.34 and 6.35 for Scenarios 1 and 2, respectively. Economic issues relevant to potentially managing skunk-variant rabies with ORV are discussed.

Resolving conflicts with Canada geese: an animal welfare perspective

The scope and significance of human conflicts with urban and suburban Canada goose populations has been growing rapidly since the mid 1980s. A lack of basic understanding about the biology and ecology of locally abundant goose populations has led, in part, to argument between opposing camps over the appropriate approaches and methodologies to resolve human-goose conflicts. Animal welfare interests have focused on the humaneness of roundup and slaughter programs, and advocated non-lethal approaches coupled with what they view as the more benign population control activity of egg addling. Some traditional wildlife managers have argued that non-lethal approaches have been tried and have failed, and that procedures such as addling do not work quickly or effectively. Differences have led to legal confrontations that absorb considerable energy and effort and may make cooperative involvement more difficult. This paper articulates some of the arguments that comprises the basis for the perspective of animal welfarists. It ends with a call for greater cooperation and involvement between all interests concerned with Canada geese.

Anestesia no transplante de rim de doador vivo no Hospital das Clínicas de Botucatu: fatores de influência para o desfecho

Pós-graduação em Anestesiologia - FMB

Avaliação do potencial citotóxico, genotóxico e mutagênico da melitina em cultura de células

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A Avaliação na sala de aula de matemática: práticas e concepções

This research aims to understand the assessment practices used by teachers at a public state school in the city of Cunha, Sao Paulo. To this end, we interviewed five mathematics teachers, who answered a questionnaire with five questions. The responses were analyzed according to the rigor of phenomenological research. To understand the investigation region, that is to say, the meaning of evaluation, we proceeded to a review of studies on the subject in authors like Buriasco (2002), Pavanello (2006), Hoffmann (1994), expressive in Mathematics Education that allows us to explain the concept of prevailing interpretation in the area. The phenomenological analysis enabled the development of three categories open revealing the concept of evaluation of teachers investigated. The first shows the review As a way to measure the knowledge acquired by the student. His interpretation leads us to understand that for some teachers, the research subjects, the assessment becomes a method to ' measure ' the knowledge acquired by the student. The second category, expressed by As a way of understanding the student's behavior in class, shows that some of the interviewees understand the evaluation as a medium that reveals and appreciates the ways of the student behave in class. Finally, the third category refers to the evaluation by means of said instruments. On this subject the claim that the assessment is through instruments such that: evidence, exercise lists, among others. In summary, interviews and categories analyzed explain the ways in which the assessment reveals the concept of implicit learning the instruments used in the evaluation practices of teachers interviewed. However, the authors read, evaluation is a necessary and permanent teaching job in teaching, which must follow step by step the process of teaching and learning. It follows, ... (Complete abstract click electronic access below)

Dose-dependent effect of Resveratrol on bladder cancer cells: Chemoprevention and oxidative stress

Background: Over 6 million people die annually in the world because of cancer. Several groups are focused on studying cancer chemoprevention approaches. Resveratrol, a polyphenol, at high dosages, has been reported as antitumor and chemopreventive. However, it has a dose-dependent effect on cell death, even on some cancer cells. Objectives: Our aim was to investigate this dose-dependent effect on human bladder carcinoma ECV304 cells during oxidative stress condition. Methods: For this purpose. ECV304 cells incubated with different Resveratrol concentrations were analyzed as for their metabolic rate, membrane permeability, DNA fragmentation, anti/proapoptotic protein levels and phosphatidylserine exposure after oxidative stress. Results: Resveratrol induced cell death at high concentrations (>20 mu M), but not at low ones (0.1-20 mu M). Pretreatment with 2.5 mu M protected the cells from oxidative damage, whereas 50 mu M intensified the cell death and significantly increased Bad/Bcl-2 ratio (proapoptotic/antiapoptotic proteins). Resveratrol was able to modulate NO and PGE(2) secretion and performed an anti-adhesion activity of neutrophils on PMA-activated ECV304 cells. Conclusions: Resveratrol at high doses induces cell death of ECV304 cells whereas low doses induce protection. Modulation of Bcl-2 protein induced by Resveratrol could be mediating this effect. This information about the role of Resveratrol on cancer alerts us about its dose-dependent effects and could lead the design of future chemoprevention strategies. Published by Elsevier Ireland Ltd.

Petrogenesis of Cenozoic volcanic rocks in the NW sector of the Gharyan volcanic field, Libya

The north-western sector of the Gharyan volcanic field (northern Libya) consists of trachytic-phonolitic domes emplaced between similar to 41 and 38 Ma, and small-volume mafic alkaline volcanic centres (basanites, tephrites. alkali basalts. hawaiites and rare benmoreites) of Middle Miocene-Pliocene age (similar to 12-2 Ma). Two types of trachytes and phonolites have been recognized on the basis of petrography, mineralogy and geochemistry. Type-1 trachytes and phonolites display a smooth spoon-shaped REE pattern without negative Europium anomalies. Type-2 trachytes and phonolites show a remarkable Eu negative anomaly, higher concentration in HFSE (Nb-Ta-Zr-Hf), REE and Ti than Type-1 rocks. The origin of Type-1 trachytes and phonolites is compatible with removal of clinopyroxene, plagioclase, alkali feldspar, amphibole. magnetite and titanite starting from benmoreitic magmas. found in the same outcrops. Type-2 trachytes and phonolites could be the result of extensive fractional crystallization starting from mafic alkaline magma, without removal of titanite. In primitive mantle-normalized diagrams, the mafic rocks (Mg#= 62-68, Cr up to 514 ppm, Ni up to 425 ppm) show peaks at Nb and Ta and troughs at K. These characteristics, coupled with low Sr-87/Sr-86(i) (0.7033-0.7038) and positive epsilon(Nd) (from +4.2 to + 5.3) features typical of the mafic anorogenic magmas of the northern African plate and of HIMU-OIB-like magma in general. The origin of the mafic rocks is compatible from a derivation from low degree partial melting (3-9%) shallow mantle sources in the spinel/gamet facies. placed just below the rigid plate in the uppermost low-velocity zone. The origin of the igneous activity is considered linked to passive lithospheric thinning related to the development of continental rifts like those of Sicily Channel (e.g.. Pantelleria and Linosa) and Sardinia (e.g., Campidano Graben) in the Central-Western Mediterranean Sea. (C) 2012 Elsevier B.V. All rights reserved.