Influência da luz LED na cultura celular de Mieloma múltiplo murino–NS1 in vitro

A monoclonal antibody (mAb) is an important tool in medical biotechnology and the production of biopharmaceuticals, especially for disease diagnosis and treatment of infections, because the antibodies have a significant advantage over chemical agents used in conventional therapies . The last thirty years the technology of production of monoclonal antibodies developed mainly the technique of obtaining in vitro, but also of their production is laborious, the cost is high. A major element of the high cost of production is the fact that the long-term culture consumes a large amount of imported inputs with high added value. A major contribution of this work is to promote cell growth more quickly and efficiently. Currently, a great race to discover new technologies and techniques to synthesize new antibodies and significantly increase the production of murine mAbs. New technologies such as laser and LED are innovations and widespread in modern life, so much so that its use has proliferated worldwide, primarily in the medical field. Recent studies show a series of results from the influence of the LED light in biological tissues such as: increasing the rate of cell proliferation, increased production rate of fibroblasts, increasing the rate of synthesis of RNA and DNA synthesis of ATP, etc. To assess the contribution of the LED in the culture of Myeloma NS1murino compared to the standard procedure. - NS1 cells were provided and followed the criteria of culture medium of the Laboratory of Cellular Engineering Center of Botucatu (POPs). The same amount of cells was grown in bottles of 25 cm2 polystyrene Tissue Culture Treated, specifically marked and kept in special medium RPMI 1640 Gibco BRL  supplemented with fetal bovine serum 10%, essential amino acids and non-essential, glucose, insulin and antibiotics. It was used in LEDs Cromatek wavelength of 630nm, 475nm and 530nm. The groups were... (Complete abstract click electronic access below)

Comércio internacional e desenvolvimento econômico: relações bilaterais Brasil-China, no período 2000-2011

Atualmente a China é um ator essencial do cenário econômico mundial, sendo também um grande demandante de matérias primas para manter seu acelerado crescimento, resultando assim em importante e estratégico para a região latinoamericana, sobretudo, por ser um grande comprador. Por sua importância econômica, estratégica e demográfica a China se destaca em relação à maioria dos países do mundo sendo não apenas um ator chave, responsável por grandes mudanças ocorridas no nível e estrutura da demanda mundial, mas também importante fonte de recursos financeiros para manter os equilíbrios internacionais. Através da discussão das relações de comércio entre Brasil e China buscamos analisar os principais resultados em termos econômicos e sociais, principalmente a atuação do Brasil enquanto ator global dado sua importância crescente na dinâmica mundial. A intenção desta pesquisa é entender as dimensões da relação com o país asiático, a partir do posicionamento do Brasil como um dos principais parceiros comerciais de China, sobretudo como exportador de produtos primários. Em termos de exportações realizadas por setores desde o Brasil para a China existe uma grande concentração nos recursos naturais de matérias primas, particularmente os minérios, alimentos, e combustíveis; trata-se de um pequeno hall de produtos com baixo nível de processamento

Campylobacter sp como perigo biológico na produção de peito de frango maturado

Pós-graduação em Microbiologia Agropecuária - FCAV

Escoamento da produção de café: uma projeção de logística

This paper seeks to show which impacts there are of the combined use of different modal for the coffee’s outflow. Nowadays is used, almost exclusively, the roadway to transport. How-ever, for this product with low added value, the types of transports best suited, in the litera-ture, are the railway and waterway. For this, is describe the development and spread of coffee throughout Brazil. Subsequently, is calculate the current cost of transporting two producing regions (Minas Gerais and Rondônia), using for that information about the freight, the social cost, and the level of losses along the way. After that is projected costs if utilizes the multi-modal model, when considering the same cost parameters used for the current model. Here-after, an analysis is made of the obtained cost, speed, consistency, capacity, availability and frequency of each transport model. Therefore, note that the multi-modal system is more ef-ficient and economical than current, based on transport by road

Crescimento e desenvolvimento econômico de Cingapura após a independência

This paper analyzes the growth and development of Singapore after its independence. For this, we made a historical analysis of the country, attributing importance in their geographic, economic, demographic, cultural and social. Singapore does not have a representative agricultural sector; its economy is based on manufacturing and the financial sector. The development of such activities was through strong planning policies and stringent regulatory institutions. Singapore economy has evolved from a trading post to a major financial center worldwide. Shows high rates of GDP per capita, which is $ 50,000 in 2011. This country has the world's busiest port for container handling. Thus, industries are based on activities with high added value and attraction of foreign companies was due to the stability of the country, which helped the growth of it

Precificação no Setor de serviço

Given the competitive reality of the globalized market, companies are compelled to develop strategic aspects of the management process, in order to ensure their survival. Among the strategic aspects is the price formation process, whose reasoning occurs mainly of adoption through cost system. Knowledge of costs and expenses provides conditions for the company to position itself strategically in the market, being able to follow the price of competition and sometimes even surpass them, called, more and more attention with regard to price formation. This study aims to identify the best price to be transferred to the market. To this end, were approached concept of pricing and its components, their strategies and pricing objectives, as well as the influence of demand on price formation, as well as the added value the brand and quality; and even a careful analysis of the price established by the said company in the service offered. We used the method of literature review and case study in the company ESA international aeronautical industry. Thus, we sought to explain about the main concepts for pricing in order to support decision making

Usinabilidade e integridade superficial de implantes dentários

Pós-graduação em Engenharia Mecânica - FEIS

Avaliação dos processos de tratamento do glicerol proveniente da produção do biodiesel

The substitution of diesel by biodiesel meets the current scenario to increase the consumption of alternative energy sources promoting sustainable development of a country. However, the production of biodiesel concurrently generates the formation of glycerine in the process is a by-product. The main application of glycerine is in the food industry, cosmetics, soaps, pharmaceuticals, among others, but these segments are not capable of absorbing the generated volume of glycerine, whereas the total volume of the biodiesel produced about 10% correspond to glycerine. Glycerine obtained from the transesterification reaction (necessary for production of biodiesel) triglycerides and alcohol contains certain impurities such as water, salts, esters, alcohol, and residual oil, which decrease the value. Thus, the purification process or the direct use of glycerine become essential to make it competitive biodiesel production process. This work aims to evaluate the different processes of purification and the use of glycerine obtained as by-product in the production of biodiesel. The research was theoretical, based on technical articles and theses published on this subject, and from these databases was established a summary of the most important processes

Catalisadores de cobre suportados em óxidos de zircônio hierarquicamente estruturados para a reação de desidrogenação de etanol

Pós-graduação em Química - IQ

Análise de impactos ambientais gerados pela implantação de loteamentos fechados de alto padrão

Pós-graduação em Engenharia Civil - FEIS

Tratamento anaeróbio-aeróbio de águas residuárias do processamento de cafeeiro em reatores biológicos

Pós-graduação em Microbiologia Agropecuária - FCAV

Expressão, purificação e caracterização de proteínas dos fitovírus SBMV e RSPaV

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de ácidos orgânicos por cepas leveduras assimiladoras de xilose

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da fermentação fúngica no teor de compostos fenólicos em casca de café robusta

Coffee husk is an abundant by-product generated by the coffee industry and it can be used for the production of-value-added phenolic compounds. Currently, this residue has no commercial use due to the presence of anti-nutritional compounds and it is returned to the soil or burned. The aim of this study was to evaluate the content of phenolic compounds in Robusta coffee husk, the adequacy of this residue as substrate for fermentation processes, as well as evaluating the influence of fungal solid state fermentation to obtain phenolic compounds from this residue. In the present study, the use of different solvents for the extraction of polyphenols was evaluated and the content was found to be in the range of 96.9-159.5 mg of galic acid (GA).g(-1) substrate, depending on the solvent used. The best solvent was acetone, therefore it was selected for extraction. Studies were carried out to evaluate the effect of solid-state fermentation in the release of phenolic compounds, using the filamentous fungi Penicillium purpurogenum. The total phenolic content increased from 159.5 up to 243.2 mg GA.g(-1) substrate as a result the solid-state fermentation.

Efeito do intervalo entre sessões de exercício de força sobre o desempenho neuromuscular

Introduction: The rest interval between bouts is a crucial variable of resistance training to ensure recovery of neuromuscular capacity. Objective: To compare the effect of rest interval between repeated bouts of resistance training on neuromuscular capacity of trained men. Methods: Eight resistance-trained men (21.6 +/- 3.3 years, 75.1 +/- 11.3 kg, 178.2 +/- 6.8 cm) performed two randomized and crossover resistance exercise bouts, repeated with rest interval of 24h or 48h. The bouts consisted of horizontal, inclined and declined bench press performed with five sets of 10 repetitions with an intensity of 70% of one repetition maximum (1RM) for each exercise. Neuromuscular performance post-exercise bout (strength, power and speed), was assessed with an accelerometer (Myotest (R)), with a load of 50% 1RM, in the bench press exercise. Results: Both sessions (24 and 48h) showed significant reduction (P < 0.05) in the neuromuscular capacity (strength, power and speed) post-exercise bout, returning to baseline values within 24h (P> 0.05). Conclusion: The results suggest that the rest interval of 24h is sufficient for recovery of neuromuscular performance in upper limbs of resistance-trained men.