Genome-wide association studies reveal genomic windows and candidate genes related to fat deposition in chickens.

2016

A câmara na escola – o cinema/documentário como ferramenta didáctica

Relatório de Estágio apresentado para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Mestrado em Ensino da História e da Geografia no 3º Ciclo do Ensino Básico e no Ensino Secundário

Studies of the assembly of the Streptococcus pneumoniae capsular polysaccharide

Dissertation presented to obtain the Ph.D degree in Biology.

O cinema como ferramenta pedagógica. Estratégias para motivar os alunos para a aprendizagem de uma língua estrangeira

Relatório de Estágio de Mestrado para a obtenção do grau de Mestre no Ensino do Português e Língua Estrangeira (Espanhol) no 3º Ciclo do Ensino Básico e Secundário

Phenetic studies on randomly amplified polymorphic DNA-polymerase chain reaction-variability of four geographical populations of Lutzomyia whitmani (Diptera: Psychodidae) in Brazil

Previous evaluation of the genetic variability of four biogeographical populations of Lutzomyia whitmani from known foci of cutaneous leishmaniasis in Brazil demonstrated two main spatial clusters: Corte de Pedra-BA, Ilhéus-BA and Serra de Baturité-CE in the first cluster, and Martinho Campos-MG in the second. Further analysis showed a high degree of homogeneity in Corte de Pedra population but not in the others, which presented a significant percentage of specimens displaced from their phenon of origin (discrepant individuals). In the present work we analyzed the frequencies of association coefficients in the matrixes of similarity per population of Lutzomyia whitmani from both sexes and the general phenograms obtained, in a more detailed study of those discrepant specimens. Populational stability was observed for Corte de Pedra population, whereas the three remaining populations showed varying degrees of heterogeneity and different displacements according to sex. Our results strongly suggested the existence of a genetic flow between the lineages North-South/North-East and Ilhéus/Serra do Baturité of Lutzomyia whitmani.

Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies

Dissertation to obtain the degree of Master in Chemical and Biochemical Engineering

Controlling gene expression in enterobacteriaceae: studies on sRNAs and strategies for synthetic biology

Dissertation presented to obtain the Master Degree in Molecular, Genetics and Biomedicine

Autos da alma : os guiões de ficção do cinema português entre 1961 e 1990

Tese de doutoramento em Ciências da Comunicação

Functional and structural studies of a mini-ferritin protein

Dissertação para obtenção do Grau de Mestre em Bioquímica

O Uso de Câmaras Digitais e a Imaginação Cinematográfica. Um Novo Cinema na Era das Câmaras Fotográficas Digitais DSLR e dos Smartphones

O presente trabalho propõe o estudo do contexto contemporâneo de grandes desenvolvimentos de dispositivos móveis e de câmaras digitais. Considerando as novas práticas na comunicação e no entretenimento audiovisual, podemos estabelecer uma relação com a nova fenomenologia de produção de vídeos com equipamentos não convencionais na medida que se estabelecem como um meio de perturbação das normas históricas de produção e distribuição do cinema convencional. É extraordinária a evolução tecnológica na era moderna, mas nem sempre nos apresenta todas as suas utilidades. Em muitos casos é através da experimentação que se vislumbram determinados benefícios do uso de um equipamento. Torna-se necessário então perceber as diferentes características que inicialmente não foram indicadas para uma determinada tecnologia. Temos de experimentar diversas variações e registar as suas vicissitudes de modo a compreender as suas possibilidades. Só assim se perfilam as funcionalidades especiais de um determinado equipamento, pela unificação do potencial criativo que se conquista através da experiência empírica. É necessário reflectir o cinema independente moderno do ponto de vista desta busca por um paradigma próprio, muito impulsionado pela liberdade criativa dos realizadores contemporâneos. O baixo custo dos novos equipamentos tecnológicos suprime à partida diversas restrições, que embora invisíveis, reduzem a liberdade de realizadores independentes sem fundos de apoio para a produção dos seus projectos. As novas tecnologias constituem uma abertura para maior actividade de produção de vídeo, possibilitando inúmeras abordagens estéticas que buscam outras soluções visuais e novas linguagens cinematográficas, além do standard.

Studies towards modified chitooligosaccharides: a new approach to NAG-NAM moiety

Dissertação para obtenção do Grau de Mestre em Bioorgânica

Dissuasão Visual: Arte, Cinema, Cronopolítica e Guerra em Directo

A percepção é essencial na cultura militar e as tecnologias da informação deram-lhe uma nova consistência. As funções perceptivas do ser humano estão agora plasmadas nas máquinas de guerra, replicando comportamentos e características fisiológicas próprias aos seres vivos. As máquinas de guerra são já sensíveis à luz, a variações térmicas ou acústicas. A essência humana, assim cristalizada sobre toda a aparelhagem de guerra, coloca em tensão os limites da diferenciação entre os campos da Arte, Cinema, Política e Guerra. Contudo, e antes ainda da era da máquina, o mundo estaria já repleto de motores naturais e poéticos. É Gilbert Simondon quem o diz, lembrando que por antecipação científica há muito que a mecanologia existe, antevendo a relação entre a indústria mais perfeita, a ciência mais bem equipada, e uma natureza no seu estado mais natural. Este será um ponto de partida verdadeiramente essencial – é que sempre tivemos a ilusão de que a técnica seria uma invenção humana quando, no fundo, foi a técnica militar e a guerra aquilo que permitiu a «fabricação do humano». Esta tese procura desvelar como o surgimento de novas tecnologias e de novos espaços para a guerra, forçaram a sua reproblematização em novas vias epistemológicas. Recuando até ao originário movimento geo-político, entroncamos numa interdefinição entre a ordem espacial e a individuação dos objectos técnicos, e procuraremos, a partir daí, saber como se concretizou a expansão da guerra moderna a todo os domínios e a todo o mundo.

Upgrade of an experimental volumetric unit for gas adsorption equilibrium studies

In this work, a volumetric unit previously assembled by the research group was upgraded. This unit revamping was necessary due to the malfunction of the solenoid valves employed in the original experimental setup, which were not sealing the gas properly leading to erroneous adsorption equilibrium measurements. Therefore, the solenoid valves were substituted by manual ball valves. After the volumetric unit improvement its operation was validated. For this purpose, the adsorption equilibrium of carbon dioxide (CO2) at 323K and 0 - 20 bar was measured on two different activated carbon samples, in the of extrudates (ANG6) and of a honeycomb monolith (ACHM). The adsorption equilibrium results were compared with data previously measured by the research group, using a high-pressure microbalance from Rubotherm GmbH (Germany) – gravimetric. The results obtained using both apparatuses are coincident thus validating the good operation of the volumetric unit upgraded in this work. Furthermore, the adsorption equilibrium of CO2 at 303K and 0 - 10 bar on Metal-Organic Frameworks (MOFs) Cu-BTC and Fe-BTC was also studied. The CO2 adsorption equilibrium results for both MOFs were compared with the literature results showing good agreement, which confirms the good quality of the experimental results obtained in the new volumetric unit. Cu-BTC sample showed significantly higher CO2 adsorption capacity when compared with the Fe-BTC sample. The revamping of the volumetric unit included a new valve configuration in order to allow testing an alternative method for the measurement of adsorption equilibrium. This new method was employed to measure the adsorption equilibrium of CO2 on ANG6 and ACHM at 303, 323 and 353K within 0-10 bar. The good quality of the obtained experimental data was testified by comparison with data previously obtained by the research group in a gravimetric apparatus.

Double-walled PLLA/PLGA particles for the sustained release of Meloxicam: preparation, characterisation and contolled release studies

There were two main objectives in this thesis investigation, first, the production, characterisation, in vitro degradation and release studies of double walled microspheres for drug release control. The second one, and the most challenging, was the production of double walled nanospheres, also for drug control delivery. The spheres were produced using two polymers, the Poly(L-lactide)Acid, PLLA, and the Poly(L-lactide-co-glycolic)Acid, PLGA.Afterwards, a model drug, Meloxicam, which is an antiinflammatory drug, was encapsulated into the particles. Micro and nanospheres were produced by the solvent extraction/evaporation method, where perfect spherical particles were obtained. By varying the polymers PLLA/PLGA mass ratio, different core and shell composition, as well as several shell and core thickness were observed. In the particles with a PLLA/PLGA mass ratio 1:1, the shell is composed by PLLA and the core by PLGA. It was also verified that the Meloxicam has a tendency to be distributed in the PLGA layer. Micro and nanoparticles were characterised in morphology, size, polymer cristalinity properties and drug distribution. Particles degradation studies was performed, where the particles in a PVA solution of pH 7,4 where placed in an incubator, during approximately 40 days, at 120rpm, and 37ºC, simulating, as much as possible, the human body environment. From these studies, the conclusion was that particles containing a PLGA shell and a PLLA core degrade more rapidly, due to the fact that PLLA is more hydrophobic than the PLGA. Concerning the drug release controlled results, done also for 40 and 50 days, they showed that the microspheres containing a shell of PLLA release more slowly than when the shell is composed of PLGA. This result was predictable, since the drug is solubilised in the PLGA polymer and so, in that case, the PLLA shell works like a barrier between the drug and the outer medium. Another positive aspect presented by this study is the lower initial burst effect, obtained when using double walled particles, which is one of the advantages of the same. In a second part of this investigation, the production of the nanospheres was the main goal, since it was not yet accomplished by other authors or investigators. After several studies, referring to the speed, time and type of agitation, as well as, the concentration and volume of the first aqueous solution of poly-vinyl-alcohol (PVA) during the process of solvent extraction/evaporation it was possible to obtain double walled nanospheres.(...)

Structural and functional studies of a high activity NiFeSe Hydrogenase

Theawareness that fossil fuels exist in limited quantities has stimulated research into energy production from renewable sources. Future energy sources! should! be! plentiful! with! negligible! impact! on! the! environment.! Hydrogen!has!the!potential!to!satisfy!these!requirements.!Nevertheless,!current! methods! of! H2! production! rely! on! nonOrenewable! resources.! Biological! H2! production! from! sunlight! or! biomass! is! an! appealing! alternative! to! current! production!methods.!!(...)