An evaluation of the trophic status of the Shen Reservoir by an analysis of phytoplankton composition and water characteristics

In an effort to evaluate the production potential of an artificial impoundment, the phytoplankton of the Shen reservoir was sampled from November 1981 to June 1982 at three stations during three periods of distinct seasonal hydrographic characteristics. The samples were subsampled and quantified. Most of the phytoplankton were identified to the species level. There were in all 53 species comprising Chlorophyceae contributing 36.70% with species of Volvox, Pediastrum, Closterium, Staurodesmus and Ankistrodesmus as dominant species in this group. The Cyanophyceae contributed 30.00% with species of Microcystis, Nostoc , and Oscillatoria as the dominant species. An analysis of temporal and spatial changes in composition and abundance of the various groups showed that these were influenced by water temperature, sampling period and station. Based on the trophic status of the most abundant species, the composition of the phytoplankton is indicative of a tropical reservoir with a moderate productivity for fish culture

Growth and age composition of northern bluefin tuna, Thunnus thynnus, caught in the Eastern Pacific Ocean, as estimated from length-frequency data, with comments on trans-Pacific migrations

ENGLISH: The spawning of Pacific northern bluefin tuna, Thunnus thynnus, takes place only in the western Pacific Ocean (WPO), but substantial numbers of the juveniles migrate to the eastern Pacific Ocean (EPO), where they remain for several months, or longer, and the.n return to the WPO. Lengthfrequency and tagging data show that many bluefin arrive in the EPO as 1-and 2-year olds, and remain there for one or two fishing seasons before returning to the WPO. The proportion of the fish which make the west-to-east migration varies among years. The numbers of 1-, 2-, 3-, 4, and >4 –year olds in the catches of the EPO are estimated for most years of the 1952-1991 period. SPANISH: EI desove del atun aleta azul del norte del Pacifico, Thunnus thynnus, ocurre solamente en el Océano Pacifico occidental (WPO), pero números substanciales de los juveniles migran al Océano Pacifico oriental (OPO), donde permanecen unos meses, 0 mas, antes de regresar al WPO. Datos de marcado y frecuencia de talla indican que muchos aletas azules llegan al OPO a 1 o 2 anos de edad, y permanecen alIi una 0 dos temporadas de pesca antes de regresar al WPO. La proporcion de los peces que migra del oeste al este varia entre anos. Se estima el numero de peces de 1, 2, 3, 4, Y>4 anos de edad en las capturas del OPO para la mayoria de los anos del periodo de 1952-1991. (PDF contains 40 pages.)

Food composition and digestive enzymes in the gut of the African electric catfish Malapterurus electricus (Gmelin 1789) (Malapteruridae)

Analyses of stomach contents of 330 Malapterurus electricus (standard length, 10.1-30.5 cm) in Mahin Lagoon (southwestern Nigeria) established it as a bottom feeder. There was a preponderance of insects accounting for >80% occurrence and >25% of total volume in stomachs of specimens, suggesting a stenophagous predatory habit. Qualitative and quantitative assays of digestive enzymes in the different regions of the gut (oesophagus, stomach, duodenum, ileum, rectum) were investigated. Carbohydrases (amylase, maltase), chitinase, proteases (pepsin, chymotrypsin, trypsin) and lipases were detected in different gut regions with different activity. The pattern of distribution and relative activity of the enzymes correlated with the predatory diet

Temperature and composition profile during double-track laser cladding of H13 tool steel

Multi-track laser cladding is now applied commercially in a range of industries such as automotive, mining and aerospace due to its diversified potential for material processing. The knowledge of temperature, velocity and composition distribution history is essential for a better understanding of the process and subsequent microstructure evolution and properties. Numerical simulation not only helps to understand the complex physical phenomena and underlying principles involved in this process, but it can also be used in the process prediction and system control. The double-track coaxial laser cladding with H13 tool steel powder injection is simulated using a comprehensive three-dimensional model, based on the mass, momentum, energy conservation and solute transport equation. Some important physical phenomena, such as heat transfer, phase changes, mass addition and fluid flow, are taken into account in the calculation. The physical properties for a mixture of solid and liquid phase are defined by treating it as a continuum media. The velocity of the laser beam during the transition between two tracks is considered. The evolution of temperature and composition of different monitoring locations is simulated.

Studies of ambient and chamber aerosol composition using the aerosol mass spectrometer

This thesis presents composition measurements for atmospherically relevant inorganic and organic aerosol from laboratory and ambient measurements using the Aerodyne aerosol mass spectrometer. Studies include the oxidation of dodecane in the Caltech environmental chambers, and several aircraft- and ground-based field studies, which include the quantification of wildfire emissions off the coast of California, and Los Angeles urban emissions.

The oxidation of dodecane by OH under low NO conditions and the formation of secondary organic aerosol (SOA) was explored using a gas-phase chemical model, gas-phase CIMS measurements, and high molecular weight ion traces from particle- phase HR-TOF-AMS mass spectra. The combination of these measurements support the hypothesis that particle-phase chemistry leading to peroxyhemiacetal formation is important. Positive matrix factorization (PMF) was applied to the AMS mass spectra which revealed three factors representing a combination of gas-particle partitioning, chemical conversion in the aerosol, and wall deposition.

Airborne measurements of biomass burning emissions from a chaparral fire on the central Californian coast were carried out in November 2009. Physical and chemical changes were reported for smoke ages 0 – 4 h old. CO₂ normalized ammonium, nitrate, and sulfate increased, whereas the normalized OA decreased sharply in the first 1.5 - 2 h, and then slowly increased for the remaining 2 h (net decrease in normalized OA). Comparison to wildfire samples from the Yucatan revealed that factors such as relative humidity, incident UV radiation, age of smoke, and concentration of emissions are important for wildfire evolution.

Ground-based aerosol composition is reported for Pasadena, CA during the summer of 2009. The OA component, which dominated the submicron aerosol mass, was deconvolved into hydrocarbon-like organic aerosol (HOA), semi-volatile oxidized organic aerosol (SVOOA), and low-volatility oxidized organic aerosol (LVOOA). The HOA/OA was only 0.08–0.23, indicating that most of Pasadena OA in the summer months is dominated by oxidized OA resulting from transported emissions that have undergone photochemistry and/or moisture-influenced processing, as apposed to only primary organic aerosol emissions. Airborne measurements and model predictions of aerosol composition are reported for the 2010 CalNex field campaign.

A biochemical and structural characterization of Drosophila neuroglian

A number of cell-cell interactions in the nervous system are mediated by immunoglobulin gene superfamily members. For example, neuroglian, a homophilic neural cell adhesion molecule in Drosophila, has an extracellular portion comprising six C- 2 type immunoglobulin-like domains followed by five fibronectin type III (FnIII) repeats. Neuroglian shares this domain organization and significant sequence identity with Ll, a murine neural adhesion molecule that could be a functional homologue. Here I report the crystal structure of a proteolytic fragment containing the first two FnIII repeats of neuroglian (NgFn 1,2) at 2.0Å. The interpretation of photomicrographs of rotary shadowed Ng, the entire extracellular portion of neuroglian, and NgFnl-5, the five neuroglian Fn III domains, is also discussed.

The structure of NgFn 1,2 consists of two roughly cylindrical β-barrel structural motifs arranged in a head-to-tail fashion with the domains meeting at an angle of ~120, as defined by the cylinder axes. The folding topology of each domain is identical to that previously observed for single FnIII domains from tenascin and fibronectin. The domains of NgFn1,2 are related by an approximate two fold screw axis that is nearly parallel to the longest dimension of the fragment. Assuming this relative orientation is a general property of tandem FnIII repeats, the multiple tandem FnIII domains in neuroglian and other proteins are modeled as thin straight rods with two domain zig-zag repeats. When combined with the dimensions of pairs of tandem immunoglobulin-like domains from CD4 and CD2, this model suggests that neuroglian is a long narrow molecule (20 - 30 Å in diameter) that extends up to 370Å from the cell surface.

In photomicrographs, rotary shadowed Ng and NgFn1-5 appear to be highly flexible rod-like molecules. NgFn 1-5 is observed to bend in at least two positions and has a mean total length consistent with models generated from the NgFn1,2 structure. Ng molecules have up to four bends and a mean total length of 392 Å, consistent with a head-to-tail packing of neuroglian's C2-type domains.

Influence of number of valves on catch composition of a fishing pot trap (Lege) in River Rima, north western Nigeria

Three types of prototype (Lege) traps with different numbers of entrance valves were evaluated in River Rima, north western Nigeria. The traps contained 4,6 and 8 valves, tagged 4-V, 6-V and 8-V respectively. The experiment was carried out in a randomized complete block design with one factor each replicated three times. Data collected on fish diversity, number biomass and sizes were subjected to descriptive statistics and analysis of variance. The results of the catch composition showed close diversity index of 0.86 for 6-V, 0.80 for 8-V and 0.60 for 4-V Lege traps. However, the number (41%) and biomass 48%) of fish caught in the 6-V Lege trap were significantly (P0.05) higher than those caught in the other traps. There was no definite trend in the sizes (length and girth) of fish caught in the traps. On the basis of species diversity, and the number and biomass of fish caught, the 6-V Lege showed preference for adoption than the other two traps. However, further studies are recommended on the appropriate mesh size net for the trap in line with the provisions of fisheries edicts

The relationship between some physicochemical parameters and plankton composition on fish production in ponds

The effect of physico-chemical parameters and plankton composition on fish production in ponds was investigated in six fish farms for eight weeks. The physicochemical parameters investigated were temperature=25-30 plus or minus C, transparency=0.45-0.57m, dissolved oxygen=3.0-10.9mg/l, pH=6.0-7.7, dissolved carbon dioxide=5.46-28.3mg/l, total alkalinity=44.37-80.0ppm, chemical oxygen demand=31.88-72.18mg/l and biological oxygen demand=0.66-48.34mg/l. Plankton composition varies and was made of four families of phytoplankton namely; Cyanophyceae, Chlorophyceae, Dinophyceae and Diatomida; and four families of zooplankton viz; Protozoa, Rotifera, Copepoda and Dinoflagellates. Farm 1 and 6 recorded the highest average weight of about 1.0kg and average total length of about 40.0cm for the two fish species. This study showed that fish yield was dependable on the quality and management of pond water characteristics

Study on the effects of three fishing baits on the catch composition of malian traps in Lake Kainji

The studies on the effects of three fishing baits on the catch composition of Malian traps in Lake Kainji were investigated. The traps were set between Monai and Taafa fishing villages in the Southern basin of the lake, baited with their respective treatment and were inspected daily for twelve days. A total of 218 fish were caught, of which the highest (54.59%) was caught by corn bran, while the lowest (11.01%) was caught by stomach content and rice bran caught 34.4%. The fish caught comprised of 15 species belonging to 8 families. There was no significant different (P>0.05) in the catch of the various baits. The weight also followed the same trend as the number of fish caught. However, both baits showed better efficiency for Alestes baremose. Tilapia zilli, S. galilaeus, Oreochromis niloticus, Labeo coubie and Distichodus rostratus than other species caught. There was a wide range between the inimum and maximum size of species caught, which showed the efficiency of the traps in capturing small size, juveniles and the adult of large fish species due to small mesh size (1") net-cover of the trap. Recommendations were made on the use of corn and rice bran as baits enhancing catch efficiency for fishes such as O.niloticus, T. zilli, T. galilaeus and D. rostratus

Influence of number of valves on catch composition of a fishing pot trap (Lege) in River Rima, north western Nigeria

Three types of prototype (Lege) traps with different numbers of entrance valves were evaluated in River Rima, north western Nigeria. The traps contained 4,6 and 8 valves, tagged 4-V, 6-V and 8-V respectively. The experiment was carried out in a randomized complete block design with one factor each replicated three times. Data collected on fish diversity, number, biomass and sizes were subjected to descriptive statistics and analysis of variance. The results of the catch composition showed close diversity index of 0.86 for 6-V, 0.80 for 8-V and 0.60 for 4-V Lege traps. However, the number (41%) and biomass (48%) of fish caught in the 6-V Lege trap were significantly (P<0.05) higher than those caught in the other traps. There was no definite trend in the sizes (length and girth) of fish caught in the traps. On the basis of species diversity, and the number and biomass of fish caught, the 6-V Lege showed preference for adoption than the other two traps. However, further studies are recommended on the appropriate mesh size net for the trap in line with the provisions of fisheries edicts

Report on the physical, bacteriological and biochemical analyses of seawater samples collected off existing sewage outfalls in the Sierra Leone River Estuary

The studies reported were undertaken as part of a wide environmental feasibility study for the establishment of a modern sewage system in Freetown. The aim of this part of the study was to determine whether the hydrological regime of the Sierra Leone River Estuary would permit the large-scale introduction of sewage into the estuary without damaging the environment. The important factors were whether: 1) there would be sufficient dilution of the sewage; 2) fleatable particles or other substances would create significant adverse effects in the estuarine ecosystem. The outfall sites are described together with the sampling stations, methods and analyses. Results include: 1) T/S profiles; 2) chemical analysis of the water. A review of literature on the Sierra Leone River Estuary is included which provides information on the plankton, benthos and fisheries. Results suggest that at certain points where local circulations occur it would be inadvisable to locate untreated sewage outfalls. Such points are frequently observed in small embayments. These studies have been of short duration but the data can serve as baseline for more extended investigations which would give a more complete picture of the seasonal patterns in the estuary.

The relationship between some physicochemical parameters and plankton composition on fish production in ponds

The effect of physico-chemical parameters and plankton composition on fish production in ponds was investigated in six fish farms for eight weeks. The physicochemical parameters investigated were temperature=25-30 plus or minus C, transparency=0.45-0.57m, dissolved oxygen=3.0-10.9mg/l, pH=6.0-7.7, dissolved carbon dioxide=5.46-28.3mg/l, total alkalinity=44.37-80.0ppm, chemical oxygen demand=31.88-72.18mg/l and biological oxygen demand=0.66-48.34mg/l. Plankton composition varies and was made of four families of phytoplankton namely: Cyanophyceae, Chlorophyceae, Dinophyceae and Diatomida; and four families of zooplankton viz: Protozoa, Rotifera, Copepoda and Dinoflagellates. Farm 1 and 6 recorded the highest average weight of about 1.0kg and average total length of about 40.0cm for the two fish species. This study showed that fish yield was dependable on the quality and management of pond water characteristics

A genetic and biochemical analysis of pre-mRNA splicing in Saccharomyces cerevisiae

Pre-mRNA splicing requires interaction of cis- acting intron sequences with trans -acting factors: proteins and small nuclear ribonucleoproteins (snRNPs). The assembly of these factors into a large complex, the spliceosome, is essential for the subsequent two step splicing reaction. First, the 5' splice site is cleaved and free exon 1 and a lariat intermediate (intron- exon2) form. In the second reaction the 3' splice site is cleaved the exons ligated and lariat intron released. A combination of genetic and biochemical techniques have been used here to study pre-mRNA splicing in yeast.

Yeast introns have three highly conserved elements. We made point mutations within these elements and found that most of them affect splicing efficiency in vivo and in vitro, usually by inhibiting spliceosome assembly.

To study trans -acting splicing factors we generated and screened a bank of temperature- sensitive (ts) mutants. Eleven new complementation groups (prp17 to prp27) were isolated. The four phenotypic classes obtained affect different steps in splicing and accumulate either: 1) pre-mRNA, 2) lariat intermediate, 3) excised intron or 4) both pre-mRNA and intron. The latter three classes represent novel phenotypes. The excised intron observed in one mutant: prp26 is stabilized due to protection in a snRNP containing particle. Extracts from another mutant: prpl8 are heat labile and accumulate lariat intermediate and exon 1. This is especially interesting as it allows analysis of the second splicing reaction. In vitro complementation of inactivated prp18 extracts does not require intact snRNPs. These studies have also shown the mutation to be in a previously unknown splicing protein. A specific requirement for A TP is also observed for the second step of splicing. The PRP 18 gene has been cloned and its polyadenylated transcript identified.

Synopses of research on shrimps: 1. The common shrimp species of Sierra Leone's coastal waters: their distribution, abundance and size composition

The authors present quantitative information on the shrimp resources of Sierra Leone waters. Four of the nine species present have been studied, of which Paenaeus duorarum notialis is dominant in the fishery. Synoptic surveys were undertaken in June 1977, and March 1978, to determine the abundance of the shrimp stock on the inshore shelf. The temperature-salinity-depth curves for the fishing ground show the existence of three water masses. The majority of fish caught were sciaenids, with some sparids also being taken. Detailed discussion of distribution and abundance of individual species of shrimp is given. The surveys have shown that the Banana Islands are the most productive shrimp grounds in the country, and the authors believe that they can support a viable shrimp industry for several years to come at present rates of exploitation

Influence of composition on the structure and properties of Fe-Pd-P and Ni-Pd-P amorphous alloys

Ternary alloys of nickel-palladium-phosphorus and iron-palladium- phosphorus containing 20 atomic % phosphorus were rapidly quenched from the liquid state. The structure of the quenched alloys was investigated by X-ray diffraction. Broad maxima in the diffraction patterns, indicative of a glass-like structure, were obtained for 13 to 73 atomic % nickel and 13 to 44 atomic % iron, with palladium adding up to 80%.

Radial distribution functions were computed from the diffraction data and yielded average interatomic distances and coordination numbers. The structure of the amorphous alloys could be explained in terms of structural units analogous to those existing in the crystalline Pd₃P, Ni₃P and Fe₃P phases, with iron or nickel substituting for palladium. A linear relationship between interatomic distances and composition, similar to Vegard's law, was shown for these metallic glasses.

Electrical resistivity measurements showed that the quenched alloys were metallic. Measurements were performed from liquid helium temperatures (4.2°K) up to the vicinity of the melting points (900°K- 1000°K). The temperature coefficient in the glassy state was very low, of the order of 10^-4/°K. A resistivity minimum was found at low temperature, varying between 9°K and 14°K for Ni_x-Pd_80-x -P₂₀ and between 17°K and 96°K for Fe_x-Pd_80-x -P₂₀, indicating the presence of a Kondo effect. Resistivity measurements, with a constant heating rate of about 1.5°C/min,showed progressive crystallization above approximately 600°K.

The magnetic moments of the amorphous Fe-Pd-P alloys were measured as a function of magnetic field and temperature. True ferromagnetism was found for the alloys Fe₃₂-Pd₄₈-P₂₀ and Fe₄₄-Pd₃₆-P₂₀ with Curie points at 165° K and 380° K respectively. Extrapolated values of the saturation magnetic moments to 0° K were 1.70 µ_B and 2.10 µ_B respectively. The amorphous alloy Fe₂₃-Pd₅₇-P₂₀ was assumed to be superparamagnetic. The experimental data indicate that phosphorus contributes to the decrease of moments by electron transfer, whereas palladium atoms probably have a small magnetic moment. A preliminary investigation of the Ni-Pd-P amorphous alloys showed that these alloys are weakly paramagnetic.