998 resultados para 1995_03262330 TM-44 4501703


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The objective of this study was to investigate the influence of lineage of oocytes donors on the number and quality of oocytes obtained through ultrasound-guided follicular aspiration in Nellore breed cows derived from two lineages of bulls (Karvadi; K and Taj-Mahal; T). Both maternal (Km and Tm) and paternal (Kp and Tp) lineages, as well as their combinations were investigated. Oocyte aspirations were repeatedly performed with an aspiration interval of 15 days in 56 donor females. Recovered cumulus oocyte-complexes (COCs) were counted, morphologically examined and classified into seven categories (grades from I to VII) according to the number of layers of the cumulus oocyte and cytoplasm appearance. The mean number of oocytes retrieved from donors of lineage Tp-Tm was significantly higher (28.23±1.92, P<0.05) than those obtained from lineages Kp-Tm, Kp-Km, and Tp-Km (21.34±1.32, 21.28±1.73, and 16.72±1.31, respectively). There was no significant difference in the mean number of recovered oocytes between donors of lineages Kp-Km and Kp-Tm, whereas animals of lineage Tp-Km yielded the lowest number of oocytes. Higher mean number of grade III oocytes was recovered from donors of lineage Kp than lineage Tp (10.11±0.66 versus 8.79±0.58, respectively), with more grade III oocytes being obtained in both lineages as compared to the others. Paternal lineage did not influence the quality of recovered oocytes in any other category, but both Kp and Tp yielded a great mean number of oocytes graded as I, II, and III (3.14±0.21; 4.93±0.33, and 10.11±0.66 versus 3.19±0.21, 5.59±0.44, and 8.79±0.58, respectively) than those classified as IV, V, VI, and VII. However, when considering the data from the maternal lineage significantly more oocytes (P<0.05) of grade I, II and III were obtained from Taj-Mahal (11.67 ± 0.67, 5.9±0.42 and 3.64±0.25, respectively) than for lineage Karvadi, with similar results for oocytes of grades IV, V, VI, and VII. Similarly to the paternal lineage, the number of oocytes of grade III was superior (P<0.05) when compared to other categories for both lineages. In conclusion, we demonstrate here a direct influence of lineage of oocyte donor on the production and quality of oocytes obtained through ultrasound-guided follicular aspiration in Nellore cows.

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Each year individuals have sought a better quality of life and work, in this case, the science of Bio-insurance needs to keep up with technological advances and the needs of society to minimize or eliminate the risks of accidents. This study evaluated the most common accidents involving health staff from 2007 to 2011 in Araçatuba-SP. 478 accidents were recorded, and 78.7% with percutaneous involvement, 9% with intact skin, 4% with no skin intact, with 7.3 mucous and 1.05% other types of exposures. Most accidents were caused by needles with lumen (68.0%), followed by other agents (14.1%), accidents with blades (8.5%) or needles without lumen (5.5%); 3.7% did not answer and 0.2% were accidents with glasses. When checking the condition in which the accident occurred, 26.0% did not specify, 25.1% occurred in the disposal and / or handling sharp objects, 12.0% at the time of drug administration, 6.9% puncture, 3.7% occurred during recapping the needle. These data show that the most common accidents are those involving needles with lumen and / or sharp objects and due to these factors, the industry of health materials have sought alternatives to minimize such accidents as needles with protective, vacuum collection . Thus, it appears that reporting accidents is important for the development of technical and / or safer materials for patients and health workers.

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This article originated in a research that was meant to map out and examine the life conditions of “travesti” (transgender) teenagers and youth living in the city of Campinas, state of São Paulo. We contacted our informants outside the environments usually identifi ed as the common spaces where members of this group spend their time and share their experiences, as a strategy that we believed could enable us to better perceive how they construct their subjectivities. As we began our ethnographic endeavors, we were surprised by the multiplicity of ways in which these young people experimented with gender and sexuality, yet which were, at the same time, accompanied by eff orts to fi t these experiments within available identity categories. Feminine boys, cross dressers and drags thus constructed their own ways of living the feminine, and in doing so, stirred our imagination regarding contemporary experiences and political struggles in the realm of sexuality.

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To assess knowledge of nursing undergraduate students about the use of contact precautions. Methodology. There were 106 nursing undergraduate students from three universities within the state of Sao Paulo. The data collection was done between April and May 2012. A questionnaire was elaborated with questions assessing knowledge regarding contact precautions. The data were submitted to statistical procedures in the package MINITAB version 16. The knowledge were rated as adequate, partially adequate and inadequate. Results. There was a predominance of females (86%) and age group between 20 and 29 years (70%). Regarding the definition of contact precaution of diseases in which these measures are used, undergraduate’s knowledge was partially adequate (92% and 44%, respectively). The knowledge was considered adequate for the preventive measures used during assistance (86%). As difficulties mentioned in the use of these measures were discomfort, lack of material, personnel and time. Conclusion. Although undergraduate students know what measures to be used in contact precaution, they do not have adequate knowledge about which diseases they should use them. The three participating universities should reflect on the quality of training for nursing students regarding biosecurity and contact precautions.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Although equines have participated in the forming and development of several civilizations around the world since their domestication 6,000 years ago in comparison to other species that have zootechnical interest, few researches have been done related to animal breeding area, especially in Brazil. Some reasons for that are difficulties associated with the species as well as operational aspects. However, developments in genetics in the last decades contributed to a better understanding of the traits related to reproduction, heath, behavior and performance of domestic animals, including equines. Recent technologies as next generation sequencing methods and the high density chips of SNPs for genotyping allowed some advances in the researches already done. These researches used basically the candidate gene strategy, and identified genomic regions related to diseases and syndromes and, more recently, the performance in sport competition and specific abilities. Using these genomic analysis tools, some regions related to race performance have been identified and based on this information; genetic tests to select superior animals for racing performance have started to be available in the market.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Despite the efforts made to improve the production of bovine embryos in vitro, their efficiency is still low, since only 30-40% of developed blastocysts are obtained from oocytes after in vitro maturation (IVM), fertilization and cultured embryos. Assisted reproductive technologies have a limiting impact due a lack of oocytes capable to fertilization.The comprehension of mechanism involved in oocyte maturation are crucial to establish a culture system that allows a larger number production of good quality embryos. The study of the early stages of oocyte and follicle development in vivo is important for a better understanding of the molecular pathways that regulate oogenesis, folliculogenesis and oocyte maturation. Thus the physiological biochemical and molecular mechanisms involved in maturation may contribute to the increased efficiency of in vitro embryo production. Therefore, the aim of this literature review is to understand the basic mechanisms that underlie oocyte maturation in cattle, since oocyte and follicle cells in vivo formation to its use in the in vitro environment.