89 resultados para subculture


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Le graffiti est depuis longtemps associé au vandalisme dans les métropoles où il prolifère. Il s’intègre au paysage urbain à un point tel que, même s’il procède d’une logique de visibilité et de promotion, nous parvenons à ne plus le remarquer. Pourtant, sa présence suscite toujours la colère des citoyens propriétaires de murs vandalisés et des autorités municipales qui, chaque année, dépensent de grosses sommes d’argent pour l’effacer. Malgré les restrictions et les amendes, le graffiti est néanmoins devenu un véritable phénomène d’art urbain : des artistes graffiteurs ont atteint la notoriété en dehors de leur sous-culture et ont pu exposer légalement leur travail, du fait d’un intérêt croissant du milieu officiel de l’art. Celui-ci contribue à faire grandir l’engouement et l’enthousiasme pour une pratique d’art urbaine. Le graffiti illustre l’expression d’une identité qui s’approprie et subvertit les surfaces urbaines de la ville, au moyen d’un nom propre fictif qui n’a aucune légitimation juridique et légale. De plus, l’application et la diffusion de la signature graffitique communiquent des valeurs qui guident et définissent toute la sous-culture du graffiti. Ces valeurs sont culturellement considérées comme masculines : le risque, le défi et la dissidence. La figure de l’artiste graffiteur apparaît ainsi comme une figure marginale et rebelle. Un tel portrait laisse alors entrevoir une culture fortement machiste, d’autant plus que les garçons qui exercent le graffiti sont beaucoup plus nombreux. Or, si les femmes artistes de la communauté graffitique représentent une minorité, c’est notamment parce que leur attrait pour une pratique illicite, nocturne et dangereuse est moindre. À partir d’une approche qui touche aux gender studies et à certains concepts traditionnels de l’histoire de l’art, nous cherchons à expliquer l’intérêt généralement plus faible des femmes pour le graffiti. Nous désirons également démontrer comment l’exercice des artistes féminines du graffiti se distingue de celui de leurs homologues masculins. De quelle manière se détermine leur expérience? Leur iconographie sert-elle à les définir? Quelles sont leurs opinions et leurs perceptions sur leur propre culture? Finalement, comment caractérisent-elles leur statut de femme artiste au sein d’une communauté "machiste"?

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L’étude qui nous concerne vise à mieux comprendre la violence en milieu carcéral. C’est à l’aide d’entrevues menées auprès de treize hommes incarcérés à l’Unité spéciale de détention, une unité canadienne spécifiquement conçue pour loger des personnes qui ont fait usage de violence intra murale ou sont soupçonné de l’avoir fait, que nous avons tenté d’atteindre l’objectif principal de ce projet. Plus spécifiquement, le point de vue des participants a été recueilli sur l’incarcération, sur les moyens utilisés afin de s’accoutumer aux défis de l’enfermement ainsi que la manière dont les individus justifient l’usage de violence dans ce contexte. Les résultats de nos analyses suggèrent que le milieu carcéral est un monde hostile et imprévisible où les individus se perçoivent constamment vulnérables à de multiples formes d’agression. Placés en hyper vigilance et orientés vers la survie, les détenus font usage de diverses stratégies d’adaptation afin de répondre aux situations difficiles qu’ils rencontrent en contexte de perte de liberté. La violence représente donc un des moyens dont disposent les individus pour survivre à l’intérieur des murs. Les diverses formes d’adaptation violente rencontrées en milieu carcéral peuvent ainsi informer sur les enjeux de survie présents dans un milieu et donc, du risque de violence ou de victimisation dans ce milieu, que cette violence soit interpersonnelle, collective, auto dirigée, axée sur la fuite ou contre les biens. Puisque l’usage de violence par une personne engendre une possibilité accrue de choisir la violence pour une autre, et ce, avec les conséquences qui s’en suivent pour les détenus et les membres du personnel, nous avons tenté d’identifier et de comprendre les diverses logiques d’action qui motivent le choix d’avoir recours à la violence dans les institutions carcérales. Or, il appert que certaines caractéristiques des individus tendent à faire augmenter le risque pour une personne d’avoir recours à la violence carcérale. De même, il semble que certaines institutions sont davantage propices à l’usage de violence que d’autres. De surcroît, des éléments appartenant aux individus et au milieu en interrelation semblent favoriser la possibilité qu’un condamné fasse usage de violence intra murale. Ainsi, le recours à la violence est davantage probable si elle est légitimée par les individus et le milieu, si le niveau d’adhésion aux valeurs des sous-cultures délinquantes des individus est élevé et si la philosophie du milieu en favorise le maintien, si des groupes influents en quête de contrôle et de pouvoir sont présents dans le milieu et qu’un individu désire y être affilié, s’il y a présence de marchés illicites comme réponse à la privation et qu’un individu y participe ou encore, si le milieu et l’individu sont pris dans l’engrenage d’un climat de survie. Par conséquent, une réflexion concernant le sentiment de sécurité préoccupant les reclus, sentiment qui est un besoin fondamental chez tous les êtres humains, un retour sur les divers modèles théoriques en fonction des données que nous avons obtenues ainsi qu’une réflexion portant sur les moyens dont disposent les individus et le milieu afin de diminuer les possibilités que la violence soit utilisée, ont été amorcées en conclusion.

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Les relations entre les policiers et les citoyens issus des communautés culturelles représentent un enjeu de taille pour le Service de Police de la Ville de Montréal (SPVM). En effet, les relations entre les policiers et les minorités ethniques sont au centre de débats et de consultations depuis plusieurs décennies. Force est de constater qu’encore aujourd’hui des tensions demeurent. Plusieurs recherches se sont penchées sur les perceptions des citoyens de leur service de police, mais peu se sont intéressées aux perceptions détaillées des jeunes hommes noirs. Il est pourtant démontré que ce groupe entretient les perceptions les moins favorables envers les policiers et que, par ailleurs, ils sont plus susceptibles d’avoir des contacts avec les policiers. Les perceptions pouvant avoir une réelle influence sur la réponse au contact policier, il appert qu'il est primordial de comprendre l’origine de ces perceptions. Ainsi, ce mémoire vise à identifier les éléments formateurs des perceptions de jeunes hommes noirs de la ville de Montréal âgés de 18 à 25 ans. Pour ce faire, l’approche qualitative a été retenue et des entretiens de type semi-directifs ont été effectués. L’analyse du discours des participants révèle principalement que les interactions personnelles avec les policiers forment les perceptions et/ou renforcent des perceptions déjà existantes. De plus, l’approche de l’agent de police lors de ce contact est mainte fois soulignée par les jeunes lors des entretiens réalisés démontrant que les premiers instants d’une interaction suffisent à l’instauration d’une perception positive ou négative. Il ressort aussi de l’étude que les expériences indirectes ont un impact certain dans la formation des perceptions surtout lorsque ces expériences ont été vécues par un proche. Ces expériences indirectes s’inscrivent d’ailleurs dans un contexte où l’appartenance culturelle du jeune et une certaine culture de quartier tendent à dicter ce que les jeunes se doivent de penser de l’organisation policière. De plus, une méconnaissance des enjeux sous-jacents aux perceptions de ces jeunes hommes par les corps policiers paraît être un obstacle à la mise en place d’interventions efficaces et significatives auprès de cette population.

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Esta tesis es el resultado de un ejercicio interdisciplinar, tanto metodológico como teórico, para observar y analizar las lógicas de construcción de subjetividad que se entraman en una iglesia evangélica y pentecostal en el sur de Bogotá llamada Comunidad Pantokrator. Allí, el cristianismo interactúa y afecta la particularidad más significativa de esta iglesia: la presencia de la subcultura del rock y del metal. En este encuentro que pareciera contradictorio entre el cristianismo y el metal, se presentan mecanismos sociales que son parte de la construcción de una subjetividad metalera y cristiana, que altera el modo en el que los asistentes de Pantokrator se entienden a sí mismos, a su congregación y a la realidad social a la que se enfrentan diariamente. En este trabajo, se relata la construcción de esos mecanismos y su operación sobre la estructura jerárquica de la iglesia, los procesos de conversión religiosa y la asunción de su fe cristiana y la forma en la que definen al mundo social que los rodea, especialmente a otras comunidades cristianas y al metal secular.

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El texto analiza el impacto de la corrupción policial presentada por los medios de comunicación desde 1993 hasta el 2012 en la estructura interna de la Policía Nacional de Colombia. En el primer capítulo se plantea un estado del arte del concepto de corrupción policial, asimismo, se incluyen las teorías bajo las cuales se ha entendido el fenómeno a nivel mundial buscando generar soluciones plausibles a un flagelo en el que se ven inmiscuidos una gran cantidad de cuerpos policiales en el mundo. En el segundo apartado, se evalúa de manera cuantitativa cómo los casos de corrupción han modificado la estructura de la Policía Nacional, pero a su vez, cómo éstas modificaciones han sido evanescentes. En el tercer capítulo, se escudriña el impacto y los cambios estructurales desde la perspectiva de los miembros del cuerpo policial, recurriendo a la recolección de datos de manera cualitativa por medio de entrevistas y encuestas. En el cuarto momento se analiza la incidencia de los directores de la policía en la lucha contra la corrupción policial y como se establece una relación entre los subalternos y los mandos medios al momento de realizar este tipo de actividades. Finalmente se plasman las conclusiones a las que se llegó posterior a la realización del trabajo investigativo.

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RE/Search Publications’ Modern Primitives (Vale and Juno 1989) changed countless lives, bringing what had been a localized and niche set of body modification practices, aesthetics and philosophies out of San Francisco to a global audience, dominating scholarly and popular discourse around body modification subculture for more than a decade afterwards. The voice of Fakir Musafar dominates the book. This article argues that modern primitives as Musafar defines them never really existed (and never could have existed) in the terms he suggests, and goes on to address an important sub-strand within Modern Primitives almost entirely ignored by critics and commentators, who have read the book as generally representative of the body modification culture as a whole. With specific reference to contributors such as infamous tattoo artist Don Ed Hardy who do not frame their practice in ‘primitive’ terms, the article concludes with a study of an alternative account presented by Vale and Juno’s book: body modification as artistic practice.

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Fan culture is a subculture that has developed explosively on the internet over the last decades. Fans are creating their own films, translations, fiction, fan art, blogs, role play and also various forms that are all based on familiar popular culture creations like TV-series, bestsellers, anime, manga stories and games. In our project, we analyze two of these subculture genres, fan fiction and scanlation. Amateurs, and sometimes professional writers, create new stories by adapting and developing existing storylines and characters from the original. In this way, a "network" of texts occurs, and writers step into an intertextual dialogue with established writers such as JK Rowling (Harry Potter) and Stephanie Meyer (Twilight). Literary reception and creation then merge into a rich reciprocal creative activity which includes comments and feedback from the participators in the community. The critical attitude of the fans regarding quality and the frustration at waiting for the official translation of manga books led to the development of scanlation, which is an amateur translation of manga distributed on the internet.  Today, young internet users get involved in conceptual discussions of intertextuality and narrative structures through fan activity. In the case of scanlation, the scanlators practice the skills and techniques of translating in an informal environment. This phenomenon of participatory culture has been observed by scholars and it is concluded that they contribute to the development of a student’s literacy and foreign language skills. Furthermore, there is no doubt that the fandom related to Japanese cultural products such as manga, anime and videogames is one of the strong motives for foreign students to start learning Japanese. This is something to take into pedagogical consideration when we develop web-based courses. Fan fiction and fan culture make it ​​possible to have an intensive transcultural dialogue between participators throughout the world and is of great interest when studying the interaction between formal and informal learning that puts the student in focus

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O objetivo deste trabalho é compreender como os consumidores de Toy Art utilizam o seu bem para constituir a sua identidade social, além de explorar este relacionamento de consumo, investigando os fatores que desencadeiam o processo de extensão de si no Toy Art. A sustentação teórica deste trabalho se deu pelo aprofundamento dos temas já levantados anteriormente por Campbell & Barbosa (2006) e McCracken (2003), em seus estudos sobre cultura e consumo; Schouten & McAlexander (1995) em sua pesquisa sobre subculturas de consumo; Hall (2005), que expôs suas teorias sobre a formação da identidade no mundo moderno; Douglas & Isherwood (2004), que estudaram os rituais de consumo; Belk (1988), com suas pesquisas sobre a extensão do self. Este estudo é de caráter exploratório, e foi conduzido com entrevistas em profundidade com o intuito de deixar emergir os sentimentos e emoções dos respondentes, para uma melhor orientação na direção das análises dos dados obtidos. Os dados foram coletados junto a 14 consumidores de Toys, de ambos os sexos, com idades entre 19 e 38 anos, residentes nos estados de Belo Horizonte, Paraná, Rio de Janeiro, Rio Grande do Sul, São Paulo e o Distrito Federal, durante os meses de julho e agosto de 2009. Para atingir os objetivos propostos, o método de pesquisa adotado foi qualitativo, com priorização do sujeito e da subjetividade, utilizando-se uma forma interpretativa para a análise dos dados. Os resultados demonstraram que os consumidores de Toy Art utilizam o seu bem para constituir sua identidade social e como uma forma de diferenciação e expressão. A extensão de si se dá exatamente durante essa busca pelo incomum, por um bem que o distinga dos demais. Conclui-se o trabalho, fazendo-se recomendações gerenciais com o intuito de beneficiar e desenvolver a indústria deste segmento.

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As organizações estão conscientes que enfrentarão complexa mudança organizacional, pois as demandas individuais na participação das decisões têm alterado os papéis desempenhados pelos gestores e seus subordinados. Neste contexto, a comunicação torna-se essencial para o exercício da influência social, para a coordenação das atividades e para a efetivação da liderança. A literatura aponta que as atitudes e os comportamentos dos funcionários são regidos por uma distribuição justa das recompensas conforme às contribuições de cada um. Assim, satisfação e atitudes positivas podem ser alcançadas por meio de um ambiente organizacional com boa comunicação, autonomia, participação, justiça distributiva e confiança. Portanto, este trabalho teve o objetivo de identificar as dimensões de Liderança e Cultura Organizacional na Criminalística da Polícia Federal, suas relações com o Clima Organizacional e como estes construtos contribuem para explicação dos problemas gerenciais. Para a coleta dos dados, foi realizada uma pesquisa quantitativa composta de 4 questionários. Dos 722 formulários enviados, 150 (20,8 %) foram respondidos: 21 (14 %) por Peritos Criminais Federais (PCF) do órgão central, 126 (84 %) por PCF das descentralizadas e 3 (2 %) por PCF de outras unidades. Os entrevistados tinham de 30 a 64 anos de idade (μ = 40,9; dp = 6,8), de 1 a 32 anos de serviço público (μ = 12,6; dp = 6,5) e de 1 a 27 anos de nomeação (μ = 7,9; dp = 3,9), e a função de chefia foi exercida por 79 (52,7 %) respondentes. Os resultados revelaram que não há diferença nas percepções de Liderança, Cultura e Clima Organizacional entre as regiões do país, rejeitando a hipótese H6 deste trabalho. Em um estudo longitudinal entre 2011 e 2013 também não houve mudanças significativas na percepção de Liderança, mostrando consistência entre as populações. Além disso, os resultados mostraram que a idade, o tempo de trabalho e o tempo de nomeação não estão associados com o Clima, logo servidores de diferentes épocas têm a mesma percepção do clima da organização. Os modelos de equações estruturais demonstraram que a Liderança, as Práticas de Liderança e o Clima são explicados fortemente por suas dimensões, e que a Cultura Organizacional afeta a Liderança e as Práticas de Liderança porém, mais significativamente, o Clima Organizacional. Tais resultados corroboram a teoria e explicam hipóteses deste trabalho. Observou-se, também, que na organização há predomínio da subcultura burocrática, baseada em controle e poder, que impacta o Clima Organizacional e, frequentemente, resulta em menor comprometimento e desempenho do servidor. A pesquisa apontou que os líderes devem encorajar a comunicação aberta para alcançar o entendimento dialógico na organização. Os achados deste estudo podem fornecer à gestão uma visão melhor para gerir seus recursos com base na legitimidade, na referência, no conhecimento e na informação, obedecendo os pressupostos do discurso ideal habermasiano.

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The present experiment used cell culture to analyze the adhesion capacity of mouse mesenchymal bone marrow cells and rat periodontal ligament to different titanium surfaces. Grade II ASTM F86 titanium discs 15mm in diameter and 1.5mm thick were used and received 2 distinct surface treatments (polished and cathodic cage plasma nitriding). The cells were isolated from the mouse bone marrow and rat periodontal ligament and cultured in α-MEM basic culture medium containing antibiotics and supplemented with 10% FBS and 5% CO2, for 72 hours at 37ºC in a humidified atmosphere. Subculture cells were cultured in a 24-well plate with a density of 1 x 104 cells per well. The titanium discs were distributed in accordance with the groups, including positive controls without titanium discs. After a 24-hour culture, the cells were counted in a Neubauer chamber. The results show that both the mouse mesenchymal bone marrow cells and rat periodontal ligament cells had better adhesion to the control surface. The number of bone marrow cells adhered to the polished Ti surface was not statistically significant when compared to the same type of cell adhered to the Ti surface treated by cathodic cage plasma nitriding. However a significant difference was found between the control and polished Ti groups. In relation to periodontal ligament cell adhesion, a significant difference was only found between the control and plasma-treated Ti surfaces. When comparing equal surfaces with different cells, no statistically significant difference was observed. We can therefore conclude that titanium is a good material for mesenchymal cell adhesion and that different material surface treatments can influence this process

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Low level laser irradiation (LLLI) has been used in Dentistry to promote wound healing and tissue regeneration. The literature shows a positive effect of LLLI on cell proliferation, but little is known about their effectiveness in promoting stem cells proliferation. The aim of this study was to evaluate the effect of LLLI on the proliferative rate of human periodontal ligament stem cells. Extracts of periodontal ligament were isolated from two third molars removed by surgical and/or orthodontic indication. After enzymatic digestion, the cells were grown in α-MEM culture medium supplemented with antibiotics and 15% fetal bovine serum. On the third subculture, the cells were irradiated with a InGaAlP-diode laser, using two different energy densities (0,5J/cm 2 - 16 seconds and 1,0J/cm² - 33 seconds), with wavelength of 660nm and output power of 30mW. A new irradiation, using the same parameters, was performed 48h after the first. A control group (non irradiated) was kept under the same experimental culture conditions. The Trypan blue exclusion test and the mitochondrial activity of the cells measured by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] essay were performed to assess the cell proliferation in the intervals of 0, 24, 48 e 72 h after irradiation. The data of cell counts were submitted to nonparametrical statistical tests (Kruskal-Wallis and Mann-Whitney), considering a confidence interval of 95%. DAPI (4 -6-Diamidino-2-phenylindole) staining of the cells was performed at 72h interval to evaluate possible nuclear morphological changes induced by LLLI. The results of this study show that the energy density of 1,0 J/cm² promoted greater cell proliferation compared to the other groups (control and 0,5 J/cm²) at intervals of 48 and 72h. The mitochondrial activity measured by MTT essay showed similar results to the Trypan blue cell counting test. The group irradiated with 1,0J/cm² exhibited a significantly higher MTT activity in the intervals of 48 and 72h, when compared to the group irradiated with 0,5J/cm². No nuclear morphological change was observed in the cells from the three groups studied. It is concluded that LLLI has stimulatory effects on the proliferation of human periodontal ligament stem cells. Therefore, the use of laser irradiation in this cell type may be important to promote future advances in periodontal regeneration

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Dental pulp stem cells have been widely investigated because of their ability to differentiate into both dental and non-dental cells, with potential use in therapies involving tissue engineering. The technique of cell cryopreservation represents a viable alternative for the conservation of these cells, since it stops reversibly, in a controlled manner, all of cell biological functions in an ultra low temperature. The present study aimed to evaluate, using in vitro experiments, the influence of a cryopreservation protocol on the biologic acti vity of stem cells from human exfoliated deciduous teeth (SHED). Cells obtained from the pulp of three deciduous teeth on end-stage exfoliation or with indicated extraction were expanded in α-MEM culture medium supplemented with antibiotics and 15% fetal bovine serum. At second subculture (P2), a group of cells were submitted to cryopreservation for 30 days in 10% DMSO diluted in fetal bovine serum, at -80º C, while the remind cells continued under normal conditions of cell culture. Cell proliferation was evaluated in both groups (not cryopreserved or cryopreserved) by Trypan blue stain essay at intervals of 24, 48 and 72h after plating. Cell cycle analysis of SHEDs submitted or not to the cryopreservation protocol was performed in the same intervals. Events related to cell death were studied by Annexyn V and PI expression under flow cytometry at the intervals of 24 and 72h. The presence of nuclear morphological changes was evaluated by DAPI staining at 72h interval. It was observed that both groups exhibited an upward cell proliferation curve, without considerable changes in cell viability throughout the experiment. The distribution of cell in the cell cycle phasis was consistent with cell proliferation in both groups. There were no nuclear morphological damages in the end range of the experiment. therefore, it is concluded that the proposed cryopreservation protocol is efficient for storing the studied cell type, allowing its use in future experimental studies

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Estudou-se a influência de diferentes irrigantes no potencial antimicrobiano da pasta de hidróxido de cálcio em dentes de cães com periodontite apical. 48 pré-molares de cães adultos tiveram suas câmaras coronárias abertas e expostas à cavidade bucal por 6 meses. Os canais radiculares foram preparados, irrigados e medicados com diferentes substâncias, de acordo com os seguintes grupos: 1) 2,5% NaOCl + CHP; 2) 2% CHX + CHP; 3) vinagre + CHP; 4) vinagre + vinagre. No grupo 4, a solução irrigante e a medicação intracanal utilizada foi o vinagre. Neste grupo, a cada 7 dias, a solução era renovada. Cada amostra foi coletada, mantendo-se o cone de papel esterilizado em posição por 1 min, e a seguir transportado e imerso em 7 mL de Letheen broth, seguido de incubação a 37ºC por 48 h. O crescimento microbiano foi analisado por dois métodos, turbidade do meio de cultura e subcultura em meio nutritivo específico (brain heart infusion). Os resultados mostraram que em todos os grupos experimentais houve crescimento microbiano após 21 dias, em diferentes percentagens: grupo 1 - 30%; grupo 2 - 30%; grupo 3 - 40%; grupo 4 - 60%. Todos os materiais testados apresentaram potencial antimicrobiano. Entretanto, o processo de cura favorecido pela pasta de hidróxido de cálcio não pode ser esquecido, uma vez que muitos estudos já demonstraram sua ação antimicrobiana.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Neural stem/progenitor cell (NSP) biology and neurogenesis in adult central nervous system (CNS) are important both towards potential future therapeutic applications for CNS repair, and for the fundamental function of the CNS. In the present study, we report the characterization of NSP population from subventricular zone (SVZ) of neonatal piglet brain using in vivo and in vitro systems. We show that the nestin and vimentin-positive neural progenitor cells are present in the SVZ of the lateral ventricles of neonatal piglet brain. In vitro, piglet NSPs proliferated as neurospheres, expressed the typical protein of neural progenitors, nestin and a range of well-established neurodevelopmental markers. Upon dissociation and subculture, piglet NSPs differentiated into neurons and glial cells. Clonal analysis demonstrates that piglet NSPs are multi-potent and retain the capacity to generate both glia and neurons. These cells expressed VEGF, VEGFR1, VEGFR2 and Neuropilin-1 and -2 mRNAs. Real time PCR revealed that SVZ NSPs from newborn piglet expressed total VEGF and all VEGF splice variants. These findings show that piglet NSPs may be helpful to more effectively design growth factor based strategies to enhance endogenous precursor cells for cell transplantation studies potentially leading to the application of this strategy in the nervous system disease and injury.