1000 resultados para análise de adaptabilidade


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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A Chromobacterium violaceum é uma beta-proteobactéria Gram-negativa comum da microbiota tropical e um patógeno oportunista para animais e humanos. A infecção causada pela C. violaceum apresenta alta taxa de mortalidade, mas os mecanismos da patogenicidade ainda não foram caracterizados. Como outros microorganismos ambientais, essa bactéria está exposta a condições externas muito variáveis, que exigem grande adaptabilidade e sistemas de proteção eficientes. Entre esses sistemas encontra-se um operon arsRBC de resistência ao arsênio, metaloide danoso à saúde humana associado a lesões de pele, doenças neurológicas e câncer. O objetivo deste trabalho foi investigar as alterações na expressão proteica de C. violaceum ATCC 12472 na presença do arsenito e caracterizar as diversas proteínas secretadas pela bactéria. As proteínas da C. violaceum foram analisadas por eletroforese bidimensional e espectrometria de massas. A análise proteômica revelou que o arsenito induz um aumento na quantidade das proteínas envolvidas na resposta ao estresse oxidativo, reparo do DNA e metabolismo energético. Entre as proteínas secretadas, foram identificados fatores de virulência (metalopeptidases, colagenase e toxinas), transportadores, proteínas de proteção contra estresses e com potencial aplicação biotecnológica. Os resultados mostraram que a C. violaceum possui um arsenal molecular de adaptação que a torna capaz de conservar suas atividades celulares e provocar lesões em outros organismos.

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The objective of this study was to identify clones of sugarcane with good stability and adaptability in the State of São Paulo, Brazil, and also identifying locations most representative for selection and experimentation. Ten clones and two commercial checks of medium-late maturation were evaluated in the first-ratoon of experiments harvested in August 2009, using bissegmented regression and AMMI (Additive Main Effects and Multiplicative Interaction Analysis) methods. The results of two methods were compared and they evidenced that the clones RB975201, RB975157, RB975932, RB975242 and RB975162 are the most promising, showing higher production when compared to the checks, higher stability observed in one or both statistical methods and broader or more specific adaptability. The environment Tarumã presented higher stability and capacity to discriminate genotypes, allowing an ordering more reliable as compared to the overall mean of the environments tested.

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Pós-graduação em Agronomia - FEIS

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Pós-graduação em Genética e Melhoramento Animal - FCAV

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The implications of modernity and globalization stimulated the establishment of a new type of organization. Resilient organizations, as they will be called, representing the twenty-first century and are aligned with the social, political and economic context for their adaptability and flexibility. The study of the case will be the communication of the NGO Periferia Legal, also linked to the plane of the creative economy because of the intangible asset it produces. The study will be focused on the ways which public relations can help in the development of the NGO by analyzing their media. Thus, the three fronts of communication from the NGO (external, internal and institutional) will be analyzed through observations and interviews and their channels are categorized into: inefficient, low efficiency , average efficiency and efficient. The media will be key to understanding the phenomenon and the degree of efficiency will be marked as it achieves the proposed and designed objectives by the project itself - through the mission and vision of the NGO - using communication. In this way, the study seeks to understand the characteristics of resilient organizations from the standpoint of public relations

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Existem vários estudos sobre a dieta de predadores de topo a uma escala local, mas o estudo da dieta de uma espécie ao longo de diferentes regiões geográficas poderá permitir a deteção de certos padrões e variações ecológicas. Para estudar a variação biogeográfica da dieta do bufo-real (Bubo bubo) ao longo do Paleártico criou-se uma base de dados baseada em 192 estudos. Pretendia-se analisar padrões em macro-escala de descritores da dieta e avaliar o efeito de diferentes condições ambientais, de descritores da paisagem e da disponibilidade de presas. Registaram-se 346813 presas de 698 espécies distintas. As principais presas são os lagomorfos e os roedores, enquanto que as aves constituem importantes presas alternativas. Temperaturas mais elevadas favorecem a diversidade trófica, mas esta diminui com a latitude e a longitude. Os resultados revelam a natureza oportunista do bufo-real e a sua elevada adaptabilidade trófica a diferentes habitats e condições ambientais; Biogeographic analysis of a top predator's diet across the Paleartic Region Summary: There are several studies about top predators' diet at a local scale, but studying a species diet across different geographic regions may allow the detection of certain patterns and variations which might influence its ecological features. We created a database of 192 papers to study the diet of the Eurasian Eagle Owl (Bubo bubo) across the Palearctic region. We analysed large scale biogeographical patterns of diet descriptors in relation to different environmental conditions, including climate, landscape and prey availability. We recorded 346816 preys from 698 different species. The main prey groups are lagomorphs and rodents, whereas birds represent important alternative prey. Higher temperatures favour a higher trophic diversity, which in turn decreases at lower latitudes and longitudes. The results reveal the opportunistic nature of the Eurasian Eagle Owl and its high trophic adaptability to different habitats and environments.

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Como a grande maioria das plantas cultivadas, a cultura do sorgo está sujeita a diferentes variações ambientais. Assim, a interação genótipos x ambientes presente no cultivo de sorgo granífero faz com que estudos de adaptabilidade e estabilidade sejam parte integrante dos programas de melhoramento vegetal. O trabalho teve por objetivo avaliar simultaneamente a adaptabilidade, estabilidade e produtividade de grãos de cultivares de sorgo avaliadas em três regiões. Foram avaliados 25 híbridos, com delineamento experimental de blocos ao acaso, com três repetições, em Sete Lagoas, Pelotas e Nova Porteirinha. Em todos os locais foram mensurados dias de florescimento, altura de plantas e produtividade de grãos. Para cada característica, foram realizados análise de variância conjunta e teste de Scott & Knott (1974). Estimativas de adaptabilidade foram realizados somente para produtividade de grãos, por ser a característica mais influenciada pelo ambiente. A seleção de genótipos baseada na produtividade, adaptabilidade e estabilidade genotípica foi baseada, no método de Lin & Binns e MHPRVG. Os híbridos 1167053, 1096009, 1167048, 1167026, 10102041, 1099034, e 1099044 se destacaram, sendo os mais estáveis e com altura de planta dentro dos limites aceitáveis em todos os ambientes. A correlação entre os dois métodos de estabilidade foi de 98%, selecionando os mesmos híbridos. Entre as testemunhas o híbridos AG 1040 apresentou melhor desempenho. Vários híbridos apresentaram produtividade de grão acima das testemunha

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Para recomendação dos genótipos adequados para as regiões de cultivo, o estudo da adaptabilidade e da estabilidade dos genótipos é imprescindível nos programas de melhoramento de plantas. O objetivo deste trabalho foi selecionar genótipos de sorgo granífero com adaptabilidade e estabilidade produtiva a diversas regiões brasileiras e verificar a associação entre os métodos de Lin & Binns (1988), Wricke (1965), Annicchiarico (1992) e Zobel et al. (1988). Os experimentos foram conduzidos em oito ambientes produtores dessa cultura, sob delineamento de blocos ao acaso com três repetições. Foram avaliados 25 genótipos, sendo 23 pertencentes ao programa de melhoramento da Embrapa Milho e Sorgo e dois híbridos comerciais. Os dados de produtividade de grãos foram submetidos à análise conjunta e, após verificar interação genótipos x ambientes significativa, foram realizadas análises de adaptabilidade e estabilidade utilizando os métodos Lin & Binns (1988), Wricke (1965), Annicchiarico (1992) e Zobel et al. (1988). Os métodos de Annicchiarico (1992) e Lin & Binns (1988) foram concordantes entre si. A associação relativamente fraca do método AMMI com Wricke (1965) e Annicchiarico (1992) permite o uso combinado desses métodos em estudos de estabilida-de e adaptabilidade. Os híbridos 1170036, 1167048 e 1170064 se destacaram por possuírem desempenho superior, alta adaptabilidade e estabilidade e podem ser alternativas para futura recomendação para cultivo.

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BACKGROUND: The model for end-stage liver disease (MELD) was developed to predict short-term mortality in patients with cirrhosis. There are few reports studying the correlation between MELD and long-term posttransplantation survival. AIM: To assess the value of pretransplant MELD in the prediction of posttransplant survival. METHODS: The adult patients (age >18 years) who underwent liver transplantation were examined in a retrospective longitudinal cohort of patients, through the prospective data base. We excluded acute liver failure, retransplantation and reduced or split-livers. The liver donors were evaluated according to: age, sex, weight, creatinine, bilirubin, sodium, aspartate aminotransferase, personal antecedents, brain death cause, steatosis, expanded criteria donor number and index donor risk. The recipients' data were: sex, age, weight, chronic hepatic disease, Child-Turcotte-Pugh points, pretransplant and initial MELD score, pretransplant creatinine clearance, sodium, cold and warm ischemia times, hospital length of stay, blood requirements, and alanine aminotransferase (ALT >1,000 UI/L = liver dysfunction). The Kaplan-Meier method with the log-rank test was used for the univariable analyses of posttransplant patient survival. For the multivariable analyses the Cox proportional hazard regression method with the stepwise procedure was used with stratifying sodium and MELD as variables. ROC curve was used to define area under the curve for MELD and Child-Turcotte-Pugh. RESULTS: A total of 232 patients with 10 years follow up were available. The MELD cutoff was 20 and Child-Turcotte-Pugh cutoff was 11.5. For MELD score > 20, the risk factors for death were: red cell requirements, liver dysfunction and donor's sodium. For the patients with hyponatremia the risk factors were: negative delta-MELD score, red cell requirements, liver dysfunction and donor's sodium. The regression univariated analyses came up with the following risk factors for death: score MELD > 25, blood requirements, recipient creatinine clearance pretransplant and age donor >50. After stepwise analyses, only red cell requirement was predictive. Patients with MELD score < 25 had a 68.86%, 50,44% and 41,50% chance for 1, 5 and 10-year survival and > 25 were 39.13%, 29.81% and 22.36% respectively. Patients without hyponatremia were 65.16%, 50.28% and 41,98% and with hyponatremia 44.44%, 34.28% and 28.57% respectively. Patients with IDR > 1.7 showed 53.7%, 27.71% and 13.85% and index donor risk <1.7 was 63.62%, 51.4% and 44.08%, respectively. Age donor > 50 years showed 38.4%, 26.21% and 13.1% and age donor <50 years showed 65.58%, 26.21% and 13.1%. Association with delta-MELD score did not show any significant difference. Expanded criteria donors were associated with primary non-function and severe liver dysfunction. Predictive factors for death were blood requirements, hyponatremia, liver dysfunction and donor's sodium. CONCLUSION: In conclusion MELD over 25, recipient's hyponatremia, blood requirements, donor's sodium were associated with poor survival.

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Errors are always present in experimental measurements so, it is important to identify them and understand how they affect the results of experiments. Statistics suggest that the execution of experiments should follow random order, but unfortunately the complete randomization of experiments is not always viable for practical reasons. One possible simplification is blocked experiments within which the levels of certain factors are maintained fixed while the levels of others are randomized. However this has a cost. Although the experimental part is simplified, the statistical analysis becomes more complex.

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Chromatography combined with several different detection systems is one of the more used and better performing analytical tools. Chromatography with tandem mass spectrometric detection gives highly selective and sensitive analyses and permits obtaining structural information about the analites and about their molar masses. Due to these characteristics, this analytical technique is very efficient when used to detect substances at trace levels in complex matrices. In this paper we review instrumental and technical aspects of chromatography-tandem mass spectrometry and the state of the art of the technique as it is applied to analysis of toxic substances in food.

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A method to quantify lycopene and β-carotene in freeze dried tomato pulp by high performance liquid chromatography (HLPC) was validated according to the criteria of selectivity, sensitivity, precision and accuracy, and uncertainty estimation of measurement was determined with data obtained in the validation. The validated method presented is selective in terms of analysis, and it had a good precision and accuracy. Detection limit for lycopene and β-carotene was 4.2 and 0.23 mg 100 g-1, respectively. The estimation of expanded uncertainty (K = 2) for lycopene was 104 ± 21 mg 100 g-1 and for β-carotene was 6.4 ± 1.5 mg 100 g-1.

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This manuscript aims to show the basic concepts and practical application of Principal Component Analysis (PCA) as a tutorial, using Matlab or Octave computing environment for beginners, undergraduate and graduate students. As a practical example it is shown the exploratory analysis of edible vegetable oils by mid infrared spectroscopy.