A functional interaction of Ku with Werner exonuclease facilitates digestion of damaged DNA

Werner syndrome (WS) is a premature aging disorder where the affected individuals appear much older than their chronological age. The single gene that is defective in WS encodes a protein (WRN) that has ATPase, helicase and 3′→5′ exonuclease activities. Our laboratory has recently uncovered a physical and functional interaction between WRN and the Ku heterodimer complex that functions in double-strand break repair and V(D)J recombination. Importantly, Ku specifically stimulates the exonuclease activity of WRN. We now report that Ku enables the Werner exonuclease to digest through regions of DNA containing 8-oxoadenine and 8-oxoguanine modifications, lesions that have previously been shown to block the exonuclease activity of WRN alone. These results indicate that Ku significantly alters the exonuclease function of WRN and suggest that the two proteins function concomitantly in a DNA damage processing pathway. In support of this notion we also observed co-localization of WRN and Ku, particularly after DNA damaging treatments.

Longitudinal relationship between cardiorespiratory fitness and academic achievement

Copyright © 2016 by the American College of Sports Medicine

Invaliditetsförsäkringkomiténs publikation no. : I[-V].

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Illustreret dansk literaturhistorie; danske digtere i det 19de aarhundrede, med en kort oversigt over aeldre dansk literatur

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Die Gletscher Schwedens im Jahre 1908,

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Studies on the vegetation of the Transcaspian lowlands,

Original imprint covered by label which reads; A. A. Knopf, New York.

Skrifter ...

Illustrated half-titles (v.6); historiated initials (v.7-8)

Dansk skuespilkunst : portraetstudier /

Michael Wiehe -- Frederik Høedt -- Fritz Hultmann -- Peter Schram -- Joachim Daniel Preisler -- Kristian Mantzuis --Fru Sødring -- Vilhelm Wiehe -- Fru Hennings -- August Bournonville -- Adolf Rosenkilde -- Johan Wiehe -- Ludvig Phister -- Olaf Poulsen -- Emil Poulsen.

Album studiosorum Academiæ Aboesis MDCXL-MDCCCXXVII. Åbo akademis studentmatrikel å nyo upprättad,

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Nye myter.

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Danmark; illustreret almanak for 1913.

"Sort og hvidt, af Johannes V. Jensen": p. 41-44.

Storhetstidens litterära krigsbyten, en kulturhistorisk-bibliografisk studie ...

Issued also in an edition with plates.

Myter og jagter /

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Vorlesungen über hydrodynamische Fernkräfte nach C. A. Bjerknes' theorie.

Available on demand as hard copy or computer file from Cornell University Library.