Karl Heinz Staudenmayer als Carlson, Rolf Kästner als Whit, Hanna Mohs als Mrs. Curley, Heinz-Georg Meyer als Slim, Szenenfoto, in: John Steinbeck: Von Mäusen und Menschen

Signatur des Originals: S 36/F11165

Rolf Henniger als Polybios, Szenenfoto, Kniestück, Profil, in: Friedrich Dürrenmatt: Herkules und der Stall des Augias, Zürich, 1963

Signatur des Originals: S 36/F11576

Pabellón alemán: EXPO'67, Montreal (Canadá): Frei Otto, Rolf Gutbrod: arquitectura textil, análisis del edificio

Pabellón alemán: EXPO'67, Montreal (Canadá): Frei Otto, Rolf Gutbrod: arquitectura textil, análisis del edificio

Conselho da República : disciplina constitucional e legal / Vitor Rolf Laube. --

Inclui bibliografia.

Considerações acerca da conformação constitucional do Tribunal de Contas / Vitor Rolf Laube. --

Inclui bibliografia.

Desapropriação urbanística / Vitor Rolf Laube. --

Inclui bibliografia.

Distrito federal : organização e natureza jurídica decorrentes da constituição de 1988 / Vitor Rolf Laube. --

Inclui notas bibliográficas.

Rolf A. Deininger

Scene showing a group of men, women, and children. The men appear to be members of the Grand Army of the Republic, possibly the Gov. Crapo Post #145

The story of Rolf and the Viking's bow /

Mode of access: Internet.

Rolf Werning / Ann-Katrin Arndt (Hrsg.): Inklusion: Kooperation und Unterricht entwickeln. Bad Heilbrunn: Klinkhardt 2013 (248 S.) [Rezension]

Rezension von: Rolf Werning/ Ann-Katrin Arndt (Hrsg.): Inklusion: Kooperation und Unterricht entwickeln. Bad Heilbrunn: Klinkhardt 2013 (248 S.; ISBN 978-3-7815-1898-8)

Immediate reconstruction following breast-conserving surgery: management of the positive surgical margins and influence on secondary reconstruction

The efficacy of breast-conserving surgery for the local control of early breast cancer has been repeatedly evidenced. Although immediate reconstruction following breast-conserving surgery has been described, little information is available regarding surgical management in reoperative settings due to positive margins. We studied the influence of intraoperatively assessed and postoperatively controlled surgical margin status on the type of breast-conserving surgery and report our results regarding complications in a reoperative breast reconstruction scenario. All patients were seen by a multidisciplinary team who recommended breast-conserving surgery. According to the breast volume, ptosis and tumor size/location, the patients were also evaluated by a plastic surgeon, who recommended reconstruction with the appropriate technique. Intraoperative assessment of surgical margins was determined by histological examination of frozen sections. The mean follow-up time was 48months. Two hundred and eighteen patients (88.5 per cent ) underwent breast-conserving surgery and immediate reconstruction. Twelve (5.5 per cent ) patients had a positive tumor margin after review of the permanent section. All patients underwent re-exploration. In 1.3 per cent , a second reconstructive technique was indicated and in 2.2 per cent a skin-sparing mastectomy with total reconstruction was performed. Our findings support the important role of the intraoperative assessment of surgical margins and its interference in the selection of reconstruction techniques and negative margins; however, it will not guarantee complete excision of the tumor. Success depends on coordinated planning with the oncologic surgeon and careful intraoperative management

Phosphatidylinositol phospholipase C mediates carbon sensing and vegetative nuclear duplication rates in Aspergillus nidulans

In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (6-7 h) in either the wild type or the plcA-deficient mutant. Only after 8 h of cultivation on glucose did the mutant strain present some decrease in nuclear duplication. However, decreased nuclear division rates were observed in the wild type when cultivated in media amended with polypectate, whereas our plcA-deficient mutant did not show slow nuclear duplication rates when grown on this carbon source, even though it requires induction and secretion of multiple pectinolytic enzymes to be metabolized. Thus, plcA appears to be directly linked to high-molecular-weight carbon source sensing.

Heterologous expression of an Aspergillus niveus xylanase GH11 in Aspergillus nidulans and its characterization and application

A xylanase was cloned from Aspergillus niveus and successfully expressed in Aspergillus nidulans (XAN). The full-length gene consisted of 890 bp and encoded 275 mature amino acids with a calculated mass of 31.3 kDa. The deduced amino acid sequence was highly homologous with the xylanase belonging to family 11 of the glycoside hydrolases. The recombinant protein was purified to electrophoretic homogeneity by anion-exchange chromatography and gel filtration. The optima of pH and temperature for the recombinant enzyme were 5.0 and 65 degrees C, respectively. The thermal stability of the recombinant xylanase was extremely improved by covalent immobilization on glyoxyl agarose with 91.4% of residual activity after 180 min at 60 degrees C, on the other hand, the free xylanase showed a half-life of 9.9 min at the same temperature. Affinity chromatography on Concanavalin A- and Jacalin-agarose columns followed by SDS-PAGE analyses showed that the XAN has O- and N-glycans. XAN promotes hydrolysis of xylan resulting in xylobiose, xylotriose and xylotetraose. Intermediate degradation of xylan resulting in xylo-oligomers is appealing for functional foods as the beneficial effect of oligosaccharides on gastrointestinal micro flora includes preventing proliferation of pathogenic intestinal bacteria and facilitates digestion and absorption of nutrients. (C) 2011 Elsevier Ltd. All rights reserved.