Identificação, caracterização e avaliação da patogenicidade de diferentes isolados de fusarium spp. para o controle de thaumastocoris peregrinus (hemiptera: thaumastocoridae)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Metodologias de pesquisa e avaliação da resistência de genótipos de amendoim a Spodoptera albula (walker, 1857) (Lepidoptera: Noctuidae)

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Dinâmica espaço-temporal da ocorrência de nematoides em seringueira

Pós-graduação em Ciência Florestal - FCA

Eficácia do flutriafol e do flutriafol + tiofanato metílico aplicados com gotas finas ou médias no controle da ferrugem asiática da soja

The aim of this study was to evaluate the influence of fine and medium droplets in the performance of flutriafol and thiophanate methyl + flutriafol for the control of Asian Soybean Rust (Phakopsora pachyrhizi Sydow & Sydow). The experiment was a 2 x 2 factorial design (2 droplet sizes x 2 fungicides) resulting in four treatments with six replications. The experimental area was set up with 24 plots (50 x 21 m). In each plot there was a central assessment area with 10 x 20 m. To the each plot there was a non-treated area placed in opposed direction to the wind. The evaluations of rust control were made by of the calculation of incidence (percentage of plants with rust), severity (level of infection), defoliations and soybean yield. The results were analyzed by the calculation of the confidence interval at 90%. The study was set up in curative control conditions with average infestation of 68.6%, average severity in the lower part of the canopy was of 35.9% and on the upper parte it was 4.57%. The results of severity, defoliation and productivity did not show statistical difference among the treatments. However, it was observed that in general there was tendency of better results with the application of flutriafol alone comparing with thiophanate methyl + flutriafol, since there was a small difference between two commercial products in the flutriafol active ingredient content (62.5 g ha-1 to the flutriafol alone and 60 g ha-1 to the thiophanate methyl + flutriafol). There was no statistical difference between fine and medium droplets. This fact can be explained by the characteristics of systemic action of the flutriafol and by the type of control made (curative). The flutriafol, being a systemic fungicide is less sensitive to the better coverage provided by the smaller droplets and, on the curative control, the amount of product deposited can became more import than coverage, mainly on the superior part of the leaves. These leaves are healthier than the inferior leaves that in general are the first to become completely compromised by the rust, losing importance to the plant. By this reason, medium droplets appear to have offered similar performance even not offering advantages in the leaf coverage. This fact may become important because in the practical side of the use of medium droplets, since this may increase the amount of time to spray, mainly because the fine and very fine droplets have more limitations related to drift and evaporation.

Resistência de genótipos de palma de óleo (Elaeis guineensis Jacq.) ao ataque de Opsiphanes invirae Hübner, 1808 (lepidoptera: nymphalidae) no Estado do Pará

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Resistência de genótipos de palma de óleo (Elaeis guineensis Jacq.) ao ataque de Opsiphanes invirae Hübner, 1808 (lepidoptera: nymphalidae) no Estado do Pará

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Toxicidade de extratos vegetais ao percevejo bronzeado do eucalipto Thaumastocoris peregrinus (hemiptera: heteroptera: thaumastocoridae) e organismos não-alvo

Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae ) is an insect from Australia which is causing severe damage to eucalyptus crops around the world. When feeding from the leaves sap, it causes bronzening, and in extreme cases, may lead to the tree death. Control methods have been studied and the most promising so far is the egg parasitoid Cleruchoides noackae (Hymenoptera: Mymaridae). Alternative products from plants with insecticidal properties could also be a viable option, and they might even be used concomitantly with C. noackae, aiming for a most effective control, but still safe for the environment. Thus, the objective of this work was to verify the action of 5% aqueous plant extracts of Matricaria chamomilla, Echinodorus grandiflorus, Punica granatum, Maytenus ilicifolia a n d Origanum majorana on T. peregrinus. In addition, we aimed to study the extracts potential toxicity to C. noackae and Gallus domesticus L., since the plant compounds might have negative effect upon the non-target organisms. At first, HPLC (High Performance Liquid Chromatography) was used to verify which phenolic compounds would be found in the plant extracts. These were tested on bronze bug adults, in confinement test (to verify the insecticidal action of the extracts) and free-choice test (to verify the repellency). The extracts that showed better results were selected for further tests with non-target organisms. Regarding C. noackae, pre-parasitism and post-parasitism, confinement and free-choice tests were performed to verify if the extracts would affect the host-choosing by the female or the development of the immature stages of the parasitoid. To verify if the extracts would be toxic to G. domesticus, the plant extracts were added to young birds feed for five days. Parameters such as weight gain, food intake, quantification of serum enzymes and histopathological analysis were carried out. HPLC analysis detected gallic, ferulic, vanillic, caffeic and cumaric acid in the extracts samples. All plant extracts tested reduced T. peregrinus survival, but E. grandiflorus, Matricaria chamomilla Maytenus ilicifolia had also a repellent effect, and were tested on the non-target organisms. None of these extracts affected neither the host choice by C. noackae nor adult emergency, when compared to the control group. In addition, the extracts did not cause alterations in any of the studied parameters. Thus, we verified that E. grandiflorus, Matricaria chamomilla and Maytenus ilicifolia have potential to be used to control T. peregrinus and are safe to C. noackae and G. domesticus.

Estudo da bioecologia de Rhombacus eucalypti Ghosh & Chakrabarti, um ácaro eriofídeo do eucalipto em Portugal

Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia - UL

Leaf anatomy of rubber-tree clones

A seringueira é uma planta de fácil reconhecimento por ser lenhosa, de porte mediano a grande, que apresenta um padrão característico de desfolha e reenfolhamento e, sobretudo, pela produção de látex. O objetivo do trabalho foi efetuar um estudo anatômico e morfológico foliar, comparando os clones RRIM 600 e GT 1 de seringueira &91;Hevea brasiliensis (Wild. ex Adr. de Juss.) Muell.- Arg&93;, desenvolvidos sob as mesmas condições edáficas e climáticas, para obtenção de informações que possam fornecer subsídios para correlações com dados fisiológicos e também diferenciar os clones em relação ao conteúdo de fibras, espessamento de tecidos do parênquima paliçádico e do parênquima lacunoso, caracterização anatômica do pecíolo, número e tamanho de estômatos e fornecer dados referentes a morfologia foliolar. Foram realizadas secções transversais na região do mesófilo, nervura central e pecíolo, seguindo-se os métodos usuais de preparação de lâminas permanentes. Foram realizadas análises biométricas de extensões de tecidos dos parênquimas paliçádico e lacunoso e contagem do número de células do parênquima lacunoso. Paralelamente foram realizadas análises biométricas para aferições de estômatos. Não houve diferenças para a altura das células epidérmicas, altura e número de camadas do parênquima lacunoso e para o comprimento e para a maior largura do limbo foliolar. Porém houve variação para a espessura das células do parênquima paliçádico, sendo que GT 1 apresentou maior espessura em relação a RRIM 600. GT1 apresentou maior número de estômatos em relação a RRIM 600, porém com menor tamanho. GT1 apresentou maior diâmetro da nervura central da folha e do pecíolo e maior quantidade de fibras de esclerênquima que RRIM 600.