Genetic variation along time in a brazilian population of Aedes aegypti (Diptera : Culicidae), detected by changes in the esterase patterns

Aedes aegypti from the Brazilian cities of Sao Jose do Rio Preto (SJ) and Goiania (GO) were analyzed as to their esterase patterns and the results were compared with data obtained about 5 years before for SJ population. Esterase bands not detected in the previous study were now observed in mosquitoes from both SJ and GO populations, being the last considered a population resistant to insecticides. Other similarities between SJ and GO populations in this study, and some differences in comparison with the previous data on SJ were observed, involving, in addition to changes in band type, changes in frequency of mosquitoes expressing them and differential gene activation during development. As it is generally true for genetic features, changes in the esterase patterns are expected to be the result of factors such as selection by environmental conditions and genetic drift. In the present case, continuous use of insecticides aiming mosquito population size control in SJ by sanitary authorities could be involved in the observed changes. Changed esterases were classified as carboxylesterases and cholinesterases, which are enzymes already shown to take part in the development of resistance in several organisms. In addition, data obtained in the elapsed time by authorities responsible for the mosquito control has shown increasing insecticide resistance of SJ population mosquitoes parallel to increase in the total amount of esterases, reinforcing the mentioned possibility.

Variação da ecogenicidade difusa em parênquima hepático homogêneo relacionada aos achados bioquímicos e hematológicos em cães

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Dessalinização do solo provocada pelo excesso do íon potássio em latossolo vermelho amarelo cultivado com alface americana (Lactuca sativa L.) irrigada sob ambiente protegido

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Dermatófitos em bovinos: incidência a suscetibilidade in vitro a drogas antifúngicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação do nível de suscetibilidade de linhagens de Aedes aegypti (Díptera: Culicidae) aos inseticidas utilizados para seu controle

Pós-graduação em Saúde Coletiva - FMB

Avaliação do efeito higrotérmico nas propriedades mecânicas de compósitos de PPS/fibras contínuas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desenvolvimento e avaliação de um modelo de assistência contínuo, paliativo e domiciliar a pacientes com câncer mamário ou ginecológico fora de possibilidade de cura

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Cetamina S(+) como adjuvante na anestesia e no tratamento da dor pós-operatória de pacientes queimados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Os contornos do Estado punitivo no Brasil

Este artigo reporta análises e conclusões formuladas a partir de observações sobre a Justiça Penal brasileira e que deram origem à tese intitulada Justiça Penal no Brasil atual: discurso democrático – prática autoritária. O objetivo desta pesquisa foi o de refletir sobre a política criminal contemporânea, voltada à ampliação da repressão e ao uso contínuo do encarceramento. Tal política, implementada no Brasil logo após a abertura política ocorrida em 1985, ajusta-se ao projeto liberal em curso no país e também em praticamente todo o Ocidente capitalista.

Variação da ecogenicidade difusa em parênquima hepático homogêneo relacionada aos achados bioquímicos e hematológicos em cães

The present study evaluated the liver with homogeneous parenchyma in dogs, with diffuse reduced echogenicity (G1), increased echogenicity (G2) and normal echogenicity (G3) by ultrasound examination associating the findings on animal profile, liver size and hematology and biochemistry results. Blood samples obtained by cephalic or jugular venipunture were submitted for hemogram and ALT, ALP, total proteins, albumin, globulin, urea and creatinine analysis. Among the 30 dogs in G1, 30 (100%) presented portal venous and gall bladder wall clarity, 23 (76.67%) presented normal liver size and edges, 15 (50%) were from 1 to 6 years old and 8 (26.67%) belonged to the lhasa apso breed. No predispositions were found according to gender, as well as no significant alterations in biochemical and hematological exams were observed in G1. The most prevalent disease found in this group was gastroenteritis (43.33%). Among the 30 dogs in G2, 27 (90%) presented hepatomegaly and rounded hepatic edges, 18 (60%) were over 9 years old, 16 (53.33%) were female and 9 (30%) belonged to the poodle breed. The laboratorial findings related to this group were increased ALT and ALP serum activity, decreased levels of total protein, albumin, globulin, erythrocytes and hematocrit, as well as leukocytosis with neutrophilia and a left shift, eosinopenia, lymphopenia and monocytosis. The most prevalent diseases found in this group were metabolic disorders (54%), such as diabetes mellitus and hyperadrenocorticism, and chronic hepatopathies (17%) due to prolonged and continuous use of corticoid and anticonvulsive drugs. Among the 30 dogs in the control group (G3), normal liver size and edges were presented in 22 (73.33%). In this group, no alterations were seen in laboratorial exams.

Projeto Memória e Envelhecimento: capacitando profissionais e aprimorando aspectos cognitivos em idosos institucionalizados

The increase in elderly population is a worldwide phenomenon and has different consequences not only in terms of health, but also in economic terms. One of those consequences is the institutionalization. The project, running in two elderly care institutions (hereafter IRLPi) located in Marília – SP aims to: a) Train multidisciplinary team to work in IRLPi’s; b) Develop and promote activities that improve life quality focusing on the preservation of mental health; c) study general and mental health conditions of patients under elderly care institution programs. The project was implemented in three phases: a) data collection and elaboration of plan of activities; b) Application of activities; and c) assessment/ analysis of results. Regarding Phase 1, data were collected from 3 forms, through which it was possible to verify: Institutional Data; Social Data and General Health. The data related to health were collected from the medication of continuous use. All data were launched into the database (Access) and subjected to the descriptive statistical analysis. Data related to mental health were confirmed from the application of clinical trials (Geriatric Depression Scale - EGD, and MMSE-T).

Estudo epidemiológico do perfil do idoso institucionalizado do interior paulista

The increase in the elderly population is a worldwide phenomenon and has different consequences not only in terms of health, but also in terms of economy. One of those consequences is the institutionalization. The understanding of this process, as well as of the reality of the institutionalized elderly is essential to develop policies of care. Goals: a) present the epidemiological profile of institutionalized elderly who live in institutions in the countryside of São PauloState and describe the development of an extension project implemented in two elderly-care institutions (IRLPI’s). Methods and procedures: data were collected in three forms, from which it was possible to verify: a) institutional data; b) social data and c) data about general and mental health. The data related to health were collected from the medication of continuous use. All data were launched into the database (Access) and subjected to the descriptive statistical analysis. Data related to mental health were confirmed from the application of clinical trials (Geriatric Depression Scale – GDS and Mini Mental State Examination: MMSE).

Tratamento da classe II divisão 2 com aparelho de Herbst após o pico de crescimento puberal

Patients with Class II division 2 malocclusion and mandibular retrusion have limited treatment options after the growth peak, such as surgical-orthodontic treatment or mandibular advancement devices. Among bite-jumping devices, the Herbst appliance allows greater increase of mandibular growth since it does not require patient compliance and allows continuous use. This case report presents the treatment of a Class II division 2 malocclusion in a patient after growth peak, performed in two stages. The first stage included the upper incisors proclination and overjet increase with multibracket appliance to benefit next stage. The second stage involved mandibular advancement using Herbst appliance aiming to correct the Class II molar relationship. The treatment resulted in a stable occlusion with periodontal health, normal functions and facial aesthetics improvement. Dental and skeletal changes arising from treatment could be assessed by cephalometric analysis and superimposition of pretreatment and post-treatment cephalometric tracings. Antero-posterior discrepancy was corrected by means of dental movement as well as by mandibular growth increment stimulated by the Herbst appliance.

Orientação semi-automática de uma sequência de pares de imagens frontais por fototriangulação a partir de fotocoordenadas extraídas pelo SIFT

Pós-graduação em Ciências Cartográficas - FCT

Determination of salicylate in blood serum using an amperometric biosensor based on salicylate hydroxylase immobilized in a polypyrrole-glutaraldehyde matrix

The use of an amperometric biosensor for the salicylate determination in blood serum is described. The biosensor is based on salicylate hydroxylase (EC 1.14.13.1) electropolymerized onto a glassy carbon-working electrode with polypyrrole and glutaraldehyde, to improve the biosensor lifetime. The hexacyanoferrate (II) was also incorporated to work as a redox mediator to minimize possible interferences. The salicylate is enzymatically converted to catechol, which is monitored amperometrically by its electrooxidation at +0.170 V versus SCE (saturated calomel electrode). Salicylate determination was carried out maintaining the ratio between β-NADH and salicylate at 4:1 (30°C). The amperometric response of the biosensor was linearly proportional to the salicylate concentration between 2.3 x 10-6 and 1.4 x 10-5 mol l- 1, in 0.1 mol l-1 phosphate buffer (pH 7.8), containing 0.1 mol l-1 KCl and 5.0 x 10-4 mol l-1 Na2H2EDTA, as supporting electrolyte. The recovery studies, in the presence of several interfering compounds, showed recoveries between 96.4 and 104.8%. The useful lifetime of the biosensor in the concentration range evaluated was at least 40 days, in continuous use. Blood serum samples analyzed by this biosensor showed a good correlation compared to the spectrophotometric method (Trinder) used as reference, presenting relative deviations lower than 7.0%. (C) 2000 Elsevier Science B.V.