The structural organization of aurein precursor cDNAs from the skin secretion of the Australian green and golden bell frog, Litoria aurea.

Aureins are a family of peptides (13-25 residues), some of which possess potent antimicrobial and anti-cancer properties, which have been classified into 5 subgroups based upon primary structural similarities. They were originally isolated from the defensive skin secretions of the closely related Australian bell frogs, Litoria aurea and Litoria raniformis, and of the 23 aurein peptides identified, 10 are common to both species. Using a recently developed technique, we have constructed a cDNA library from the defensive secretion of the green and golden bell frog, L. aurea, and successfully cloned a range of aurein precursor transcripts containing entire open-reading frames. All open-reading frames consisted of a putative signal peptide and an acidic pro-region followed by a single copy of aurein. The deduced precursor structures for the most active aureins (2.2 and 3.1) confirmed the presence of a C-terminal amidation motif whereas that of aurein 5.3 did not. Processed peptides corresponding in molecular mass to aureins 2.2, 2.3, 2.5, 3.1 and 5.3 were identified in the same secretion sample using LC/MS. The application of this technique thus permits parallel peptidomic and transcriptomic analyses on the same lyophilized skin secretion sample circumventing sacrifice of specimens of endangered herpetofauna.

Bell - Sloman Wedding Day Photo, 1939

This photograph was taken on the wedding day of Richard Nelson Bell and Iris Sloman in 1939. Pictured in the photograph, from left, are: Charles Bell, father of Richard Nelson Bell; Josephine Sloman, mother of Iris; Richard Nelson Bell; Iris Sloman Bell; Mary Bell, mother of Richard Nelson Bell; and Albert Sloman, father of Iris. This family photo was in the possession of Rick Bell, of St. Catharines, Ontario. The Sloman - Bell ancestry includes escaped Black slaves from the United States.

Richard Nelson Bell - Iris Sloman Wedding Day Photo, 1939

A black and white photograph taken in 1939 on the occasion of the marriage of Richard Nelson Bell to Iris Sloman. Pictured in the photograph, from left to right, are: Bert Sloman; Richard Nelson Bell; Iris Sloman Bell; and Helen Beatty Sloman. This photograph was in the possession of Rick Bell, the son of Richard Nelson and Iris Bell. The Bell - Sloman family descends from former Black slaves from the United States.Bert Sloman (Albert Edward Sloman) passed away in 1986 at Kitchener-Waterloo, Ontario. His wife Vera Matilda Sloman passed away January 4, 2011 at Cambridge Memorial Hospital. They had a son, Ron Sloman.

Photograph of Robert Bell, Airborne Regiment, World War II [n.d.]

A photograph of Robert Bell (kneeling, front row, third left) with his airborne regiment in the Second World War. The date and location of the photo is unknown. This photograph was among those recovered from the attic of Iris Sloman Bell, of St. Catharines, in the 1980s and kept in the possession of her son, Rick Bell. The Bell- Sloman family is descended from former Black slaves from the United States.

Richard and Iris Bell Wedding Day, 1939

Richard and Iris (Sloman) Bell are pictured on the grounds of unknown gardens, possibly in Niagara Falls, on their wedding day in 1939. This photo was among the family memorabilia in the possession of their son, Rick Bell, of St. Catharines.

Vera Sloman, Richard Bell, Iris Sloman Bell and Bert Sloman [n.d.]

This black and white family photo is undated yet believed to be in 1939 on the wedding day of Richard and Iris Bell. Pictured in this photo are, from right, Vera, Richard Bell, Iris Sloman Bell, and Bert Sloman. This photo was in the possession of Rick Bell, of St. Catharines, son of Richard and Iris. The Bell - Sloman families have descendants who are former African American slaves.Bert Sloman (Albert Edward Sloman) passed away in 1986 at Kitchener - Waterloo, Ontario. His wife, Vera Matilda Sloman, passed away January 4, 2011 at Cambridge Memorial Hospital. They had a son, Ron Sloman.

Trapped in the Bell Jar: Mental Illness in College Students

This is a magazine article that explores the rising problem of mental health in college students, focusing on Connecticut. It explores the experiences of three college students dealing with depression and bipolar disorder, a family who lost a child to suicide, and the measures taken by colleges in Connecticut to curb the problem.

The APC variants I1307K and E1317Q are associated with colorectal tumors, but not always with a family history

Classical familial adenomatous polyposis (FAP) is a high-penetrance autosomal dominant disease that predisposes to hundreds or thousands of colorectal adenomas and carcinoma and that results from truncating mutations in the APC gene. A variant of FAP is attenuated adenomatous polyposis coli, which results from germ-line mutations in the 5′ and 3′ regions of the APC gene. Attenuated adenomatous polyposis coli patients have “multiple” colorectal adenomas (typically fewer than 100) without the florid phenotype of classical FAP. Another group of patients with multiple adenomas has no mutations in the APC gene, and their phenotype probably results from variation at a locus, or loci, elsewhere in the genome. Recently, however, a missense variant of APC (I1307K) was described that confers an increased risk of colorectal tumors, including multiple adenomas, in Ashkenazim. We have studied a set of 164 patients with multiple colorectal adenomas and/or carcinoma and analyzed codons 1263–1377 (exon 15G) of the APC gene for germ-line variants. Three patients with the I1307K allele were detected, each of Ashkenazi descent. Four patients had a germ-line E1317Q missense variant of APC that was not present in controls; one of these individuals had an unusually large number of metaplastic polyps of the colorectum. There is increasing evidence that there exist germ-line variants of the APC gene that predispose to the development of multiple colorectal adenomas and carcinoma, but without the florid phenotype of classical FAP, and possibly with importance for colorectal cancer risk in the general population.

The genealogist's guide to printed pedigrees. Being a general search through genealogical, topographical, and biographical works relating to the United Kingdom, together with references to family histories, peerage claims, etc.

LC copy replaced by microfilm.

The Moran family ; 200 years in Detroit.

Mode of access: Internet.

Memorials of the Civil War: comprising the correspondence of the Fairfax family with the most distinguished personages engaged in that memorable contest. Now first published from the original manuscripts /

Mode of access: Internet.

The history of the revolutions in England under the family of the Stuarts, : from the year 1603, to 1690. In three books; wherein are contained many secret memoirs relating to that family, and the last great revolution, anno 1688. /

Head- and tail-pieces; initials.

The Expanded Human Kallikrein (KLK) gene family : genomic organisation, tissue specific expression and potential functions

The tissue kallikreins are serine proteases encoded by highly conserved multigene families. The rodent kallikrein (KLK) families are particularly large, consisting of 13 26 genes clustered in one chromosomal locus. It has been recently recognised that the human KLK gene family is of a similar size (15 genes) with the identification of another 12 related genes (KLK4-KLK15) within and adjacent to the original human KLK locus (KLK1-3) on chromosome 19q13.4. The structural organisation and size of these new genes is similar to that of other KLK genes except for additional exons encoding 5 or 3 untranslated regions. Moreover, many of these genes have multiple mRNA transcripts, a trait not observed with rodent genes. Unlike all other kallikreins, the KLK4-KLK15 encoded proteases are less related (25–44%) and do not contain a conventional kallikrein loop. Clusters of genes exhibit high prostatic (KLK2-4, KLK15) or pancreatic (KLK6-13) expression, suggesting evolutionary conservation of elements conferring tissue specificity. These genes are also expressed, to varying degrees, in a wider range of tissues suggesting a functional involvement of these newer human kallikrein proteases in a diverse range of physiological processes.