Genetic and phylogenetic analysis of glycoprotein of rabies virus isolated from several species in Brazil

Genetic and phylogenetic analyses of the region containing the glycoprotein (G) gene, which is related to pathogenicity and antigenicity, and the G-L intergenic region were carried out in 14 Brazilian rabies virus isolates. The isolates were classified as dog-related rabies virus (DRRV) or vampire bat-related rabies virus (VRRV), by nucleoprotein (N) analysis. The nucleotide and amino acid (AA) homologies of the area containing the G protein gene and G-L intergenic region were generally lower than those of the ectodomain. In both regions, nucleotide and deduced AA homologies were lower among VRRVs than among DRRVs. There were AA differences between DRRV and VRRV at 3 antigenic sites and epitopes (IIa, WB+ and III), suggesting that DRRV and VRRV can be distinguished by differences of antigenicity. In a comparison of phylogenetic trees between the ectodomain and the area containing the G protein gene and G-L intergenic region, the branching patterns of the chiropteran and carnivoran rabies virus groups differed, whereas there were clear similarities in patterns within the DRRV and VRRV groups. Additionally, the VRRV isolates were more closely related to chiropteran strains isolated from Latin America than to Brazilian DRRV. These results indicate that Brazilian rabies virus isolates can be classified as DRRV or VRRV by analysis of the G gene and the G-L intergenic region, as well as by N gene analysis.

Genetic characterization of rabies viruses isolated from frugivorous bat (Artibeus spp.) in Brazil

In Latin America, rabies cases related to frugivorous bats have been reported since 1930's. Recently, two viruses isolated from Artibeus lituratus were proved to be vampire bat variants by monoclonal antibodies panels [2], but their genetic information is not well known. In this report, four rabies viruses were isolated from frugivorous bats (Artibeus spp.) in Brazil and their nucleoprotein gene sequences were determined. These isolates were found to be genotype 1 of lyssavirus and showed the maximum nucleotide sequence homology of 97.6-99.4% with vampire bat-related viruses in Brazil [6]. These results indicate that the Brazilian frugivorous bat rabies viruses in this study are closely related to vampire bat-related viruses that play a main role in rabies virus transmission to livestock in Brazil.

Modelling thermodynamic properties of banana waste by analytical derivation of desorption isotherms

Banana is an agricultural product of great economic importance for various developing countries. The relationship between moisture content and water activity provides useful information for the processing and storage of banana waste. The water activity and moisture content of three banana (Mussa spp. Haploid AAB cv. Nanica) waste items were analyzed to determine the desorption isotherms at six different temperatures (20, 30, 40, 50, 60 and 70°C). The desorption isotherms of the peel, pedicel and pulp of overripe bananas were determined in wide ranges of moisture content (0.001-6.360 kg kg-1 d.b.) and water activity (0.02-0.907). The theoretical GAB model was used for modelling the desorption isotherms. An analytical solution of the Clausius-Clapeyron equation was proposed to compute the isosteric heat of sorption, the differential entropy and Gibbs' free energy by way of the GAB model when the effect of temperature on the hygroscopic equilibrium was considered. © 2012 de Gruyter. All rights reserved.

Aplicações de secagem para o aproveitamento de resíduos da banana, visando sua aplicação na indústria

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização morfológica e atributos de qualidade dos frutos de acessos de bananeira em clima subtropical

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação de genótipos de bananeira (Musa sp.) em Botucatu -SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Radioatividade e hidroquímica em águas subterrâneas do município de Botucatu (SP)

The main objective of this study is to determine the activity of Po-210, Pb-210 and gross alpha/beta radioactivity in groundwater collected in Botucatu, municipality of the São Paulo state, Brazil. Samples were collected in wells to perform the radiochemical analysis. Most wells are registered in DAEE, Sabesp or CPRM. The activity of polonium was obtained by the method of alpha spectrometry, held in LABIDRO at UNESP, Rio Claro. All samples showed radioactivity values below the maximum allowed by WHO, that are: gross alpha radioactivity = 0,5 Bq/L, gross beta radioactivity = 1,0 Bq/L, Po210 = 0,2 Bq/L and Pb210 = 0,1 Bq/L. The results of hydrochemical analysis were compared with the 1469 Ordinance of the Health Ministry, and all values are bellow the maximum allowed. The data also allowed obtain the samples classification using the Piper diagram. The samples AAB, Quinta do Manacás, Faz. Quatro Irmãs, Sesi, Sitio São José and ITE are classified as calcic bicarbonate; the samples Staroup and Caio are potassic; the samples Banespa and BTC are bicarbonate and mixed interms of dissolved cations; Sitio das Palmeiras sample is sodium bicarbonate; the sample Faz. São Paulo is chlorinated calcic; the sample Sítio Btu-Pardinho chlorinated and mixed interms of dissolved cations

Aspectos da fisiopatologia da sepse em Piaractus mesopotamicus induzida por Aeromonas hydrophila

Pós-graduação em Medicina Veterinária - FCAV

Desenvolvimento micelial de Lentinula edodes em meios de cultivo à base de diferentes substratos orgânicos

The radial mycelial growth of Lentinula edodes (Berk.) Pegler, strain LE-96/13, was studied in culture media prepared with organic residues extract, by using substrates prepared with pineapple (Ananas comosus (L.) Merril) crown, Astrocaryum aculeatum Meyer peel, Theobroma grandiflorum Schum shell, Musa sp. (genomic group AAB, subgroup Pacovan) peel, and Musa sp. (genomic group AAB, subgroup Prata) peel, with three supplementation levels with wheat bran (0, 10 and 20%), and incubated at 25ºC. The experimental design was totally randomized, in a 5×3 factorial scheme, adding up 15 treatments with 4 repetitions, and each repetition corresponding to a Petri dish. The diameter of the colony was evaluated daily during nine days of incubation. After that period, it was verified that the highest mycelial growth averages of strain LE-96/13 of L. edodes were found in culture media prepared with T. grandiflorum Schum shell (whose supplementation with wheat bran was favorable for Mushroom development) and A. aculeatum Meyer peel (whose supplementation did not favor the mycelial growth of L. edodes in relation to the medium not supplemented).

¿Jornadas de acogida en la alfabetización informacional?

La alfabetización informacional debería ser un elemento estratégico de las universidades en el proceso de adaptación de los estudios universitarios al EEES. Esto obliga a las bibliotecas a diseñar planes de formación que abarquen todos los niveles educativos. La adaptación de las jornadas de acogida al EEES pasa por la adaptación de su contenido al sistema de crédito europeo (ECTS) en aquellas universidades que hasta ahora han dado créditos por las mismas y, por supuesto, por la utilización del entorno virtual. En nuestra propuesta intentamos conjugar las enseñanzas presenciales con las virtuales diseñando para el campus virtual herramientas como archivos pdf multimedia, flash, vídeo.

Are land sparing and land sharing real alternatives for European agricultural landscapes?

There is a lively debate on whether biodiversity conservation and agricultural production could be better reconciled by land sparing (strictly separating production fields and conservation areas) or by land sharing (combining both, agricultural production and biodiversity conservation on the same land). The debate originates from tropical countries, where agricultural land use continues to increase at the expense of natural ecosystems. But is it also relevant for Europe, where agriculture is withdrawing from marginal regions whilst farming of fertile lands continues to be intensified? Based on recent research on farmland biodiversity we conclude that the land sharing – land sparing dichotomy is too simplistic for Europe. Instead we differentiate between productive and marginal farmland. On productive farmland, semi-natural habitats are required to yield ecosystem services relevant for agriculture, to promote endangered farmland species which society wants to conserve even in intensively farmed regions, and to allow migration of non-farmland species through the agricultural matrix. On marginal farmland, high-nature value farming is a traditional way of land sharing, yielding high quality agricultural products and conserving specialized species. To conserve highly disturbance-sensitive species, there is a need for nature reserves. In conclusion, land sparing is not a viable olution for Europe in both productive and marginal farmland but because of different reasons in each type of farmland.

Influence of allelic prolamin variation and localities on durum wheat quality

Thirty-seven varieties of a Mediterranean durum wheat collection grown in Tunisia and Spain were analysed for their allelic composition in prolamins, as well as their protein concentration, sodium dodecyl sulphate sedimentation (SDSS) test and mixograph parameters. Genotype was a greater source of variation in all measurements than locality. Uncommon high and low molecular glutenin subunits (HMW-GS and LMW-GS) were found (V and 2? subunits at Glu-A1, 13 þ 16 at Glu-B1, 5* subunit and ax allele at Glu-A3). The rare combinations 2 þ 4þ14 þ 18 and 8 þ 9þ13 þ 16þ18 subunits at the Glu-B3 locus were found. Glu-A3ax had a positive influence on SDSS and mixograph parameters. Of all the prolamins, those that have the B-LMW-GS composition aaa (for Glu-A3, Glu-B3 and Glu-B2 loci, respectively), when associated with the Glu-A1c and Glu-B1d gave the best semolina quality. By contrast, semolina quality is poor when this same composition is associated with the Glu-A1c and Glu-B1e and even poorer when associated with the Glu-A1c and Glu-B1f. In addition, the cultivars with B-LMW-GS allelic composition aab (for Glu-A3, Glu-B3 and Glu-B2 loci, respectively), when associated with the Glu- A1c and Glu-B1d, gave high quality, whereas when associated with the Glu-A1c and Glu-B1e or with Glu- A1o and Glu-B1f, the quality was very poor.