tagO is involved in the synthesis of all anionic cell-wall polymers in Bacillus subtilis 168.

Sequence homologies suggest that the Bacillus subtilis 168 tagO gene encodes UDP-N-acetylglucosamine:undecaprenyl-P N-acetylglucosaminyl 1-P transferase, the enzyme responsible for catalysing the first step in the synthesis of the teichoic acid linkage unit, i.e. the formation of undecaprenyl-PP-N-acetylglucosamine. Inhibition of tagO expression mediated by an IPTG-inducible P(spac) promoter led to the development of a coccoid cell morphology, a feature characteristic of mutants blocked in teichoic acid synthesis. Indeed, analyses of the cell-wall phosphate content, as well as the incorporation of radioactively labelled precursors, revealed that the synthesis of poly(glycerol phosphate) and poly(glucosyl N-acetylgalactosamine 1-phosphate), the two strain 168 teichoic acids known to share the same linkage unit, was affected. Surprisingly, under phosphate limitation, deficiency of TagO precludes the synthesis of teichuronic acid, which is normally induced under these conditions. The regulatory region of tagO, containing two partly overlapping sigma(A)-controlled promoters, is similar to that of sigA, the gene encoding the major sigma factor responsible for growth. Here, the authors discuss the possibility that TagO may represent a pivotal element in the multi-enzyme complexes responsible for the synthesis of anionic cell-wall polymers, and that it may play one of the key roles in balanced cell growth.

Divers cartonnages de momie. Egyptien 168

Isis ailée coiffée du disque solaire surmontant onze génies funéraires diposés de part et d’autre d’une colonne de hiéroglyphes contenant une formule funéraire au nom d’un Osiris non conservé.

Traumatismo torácico: estudo retrospectivo de 168 casos

OBJETIVO: Avaliar o atendimento ao paciente portador de traumatismo torácico. MÉTODO: Estudo retrospectivo de 168 casos de trauma do tórax, com ênfase na abordagem inicial, conduta operatória e cuidados pós-operatórios. RESULTADOS: Dos 168 pacientes, 120 eram do sexo masculino e a média de idade encontrada foi de 35,5 anos. Dez pacientes foram toracotomizados de urgência, quatro por ferimento cardíaco, quatro devido à lesão de vasos pulmonares, um por lesão do saco pericárdico e outro do pedículo pulmonar. Os demais tiveram suas condições clínicas corrigidas através de simples drenagem torácica do hemitórax atingido. Ocorreram dois óbitos não cirúrgicos e um devido à insuficiência respiratória. CONCLUSÕES: O traumatismo torácico, além de ser muito freqüente, é na maioria das vezes, de fácil resolução. Podem ocorrer, no entanto, lesões torácicas graves envolvendo órgãos vitais que merecem intervenção mais agressiva e perícia técnica, com suporte hospitalar de alto nível.

Resolución N° 168 del 20 de septiembre de 2010 ¿A la cámara de comercio le es dable controlar el requisito de las mayorías para la inscripción de un acta de nombramiento de administradores de una sociedad limitada?

Servicios registrales

HG 168.50-168.60

Brown sediment with three main domains; two different fine grained domains and one coarse grained domain. The fine grained domains can be distinguished based on the abundance of organic material (darker). Both domains contain lineations. The coarse grained domain contains clasts ranging from small to medium in size and angular to rounded in shape. Rotation structures, lineations and comet structures can be seen in this domain. It also contains some fractured grains.

Postcard - H.K. Woodruff's Residence at 168 Ontario Street, St. Catharines

A black and white postcard of H.K. Woodruff's residence at 168 Ontario Street, St. Catharines, Ontario. It is described on the reverse by R. Band in 1977.