Adenovirus-mediated urokinase gene transfer induces liver regeneration and allows for efficient retrovirus transduction of hepatocytes in vivo.

Retrovirus-mediated gene transfer into hepatocytes in vivo results in long-term gene expression. Limitations include the need to remove two-thirds of the liver and the relatively low frequency of gene transfer. To increase gene transfer without surgical hepatectomy, mouse hepatocytes were transduced in vivo with a recombinant adenovirus that transiently expressed urokinase, resulting in high rates of asynchronous liver regeneration. During the regenerative phase, in vivo retroviral-mediated gene transfer in hepatocytes resulted in 5- to 10-fold greater transduction efficiencies than that obtained by conventional partial hepatectomy. In 3-4 weeks, the architecture and microscopic structure of the recipient livers were normal. The two-viral system of achieving permanent transgene expression from hepatocytes in vivo offers an alternative approach to current ex vivo and in vivo gene-transfer models.

Commonplace book of Hector Orr, 1789-1804

Hector Orr began recording entries in this commonplace book during his first year as a student at Harvard and continued writing in the volume sporadically until 1804. The entries written while he was a student, from 1789 to 1792, include themes written on the following topics: Time, Discontent, Patriotism, Virtue, Conscience, Patience, Avarice, Compassion, Mortality, Self-knowledge, Benevolence, Morning, Anger, Profanity, Bribery, Autumn and Winter, Hermitage, Conscience and Anticipation. He also wrote detailed entries about the forensic disputations in which he and his classmates participated, explaining both the affirmative and negative positions. One of these disputations involved discussion of the Stamp Act, which was then quite recent history. Orr's entries about the disputations list the names of students involved and specify their position in the argument.

Notes on cases in Lennox and Worcester, 1789

Contains notes on cases before the Supreme Court in Lennox and Worcester, Massachusetts relating to counterfeiting, libel, and fraud.

Student mathematical notebook of Thomas Noyes, 1793

This sewn volume contains Noyes’ mathematical exercises in geometry; trigonometry; surveying; measurement of heights and distances; plain, oblique, parallel, middle latitude, and mercator sailing; and dialing. Many of the exercises are illustrated by carefully hand-drawn diagrams, including a mariners’ compass and moon dials.

An oration upon genius : Commencement address, 1797

Sixteen-page paper notebook containing a handwritten copy of an essay titled "An Oration upon Genius" composed by AM candidate Joseph Perkins for the 1797 Harvard Commencement. The verso of the last page contains the handwritten title: "Oration on Genius first Copy. July 17th Commencement 1797." The essay discusses themes of republicanism and education and begins, "To mark the peculiar operation of liberty on mental exertion and improvement..."

Student mathematical notebook of Ebenezer Hill, 1785

This mathematical notebook of Ebenezer Hill was kept in 1795 while he was a student at Harvard College. The volume contains rules, definitions, problems, drawings, and tables on arithmetic, geometry, trigonometry, surveying, calculating distances, and dialing. Some of the exercises are illustrated by hand-drawn diagrams, including some of buildings and trees.

Student notes of Elijah Dunbar, 1792-1793

Three unlined pages with notes written by Harvard undergraduate Elijah Dunbar. The documents consist of two pages of chemistry notes compiled in September 1792 when Dunbar was a junior and an undated, untitled list of theological themes. The chemistry notes include a summary of the discipline and a set of laws regarding the "affinity of composition." The verso of the second page was later annotated: "Borrow- He that discerneth Youth & Beau[ty] Elij. Dunar 2'd 1793. Rec'd David Tappan, Professor of Divinity in the University--Elijah Dunbar, jun." followed by a list of students identified as "Alchemists" in the "Ridiculous Society": Joseph Perkins, Isaac Braman, William Biglow, and Elijah Dunbar. The second document is an untitled list of 27 theological themes beginning "1. Doctrine of the Trinity," and ending "27. Family worship," and may refer to sermon or lecture topics.

Student mathematical notebook of William Emerson Faulkner, 1795

Leather hardcover notebook with unruled pages containing the handwritten mathematical exercises of William Emerson Faulkner, begun in 1795 while he was an undergraduate at Harvard College. The volume contains rules, definitions, problems, drawings, and tables on geometry, trigonometry, surveying, calculating distances, sailing, and dialing. Some of the exercises are illustrated by unrefined hand-drawn diagrams, including some of buildings and trees.

Student mathematical notebook of William Tudor, 1795

Notebook containing the handwritten mathematical exercises of William Tudor, kept in 1795 while he was an undergraduate at Harvard College. The volume contains rules, definitions, problems, drawings, and tables on geometry, trigonometry, surveying, calculating distances, sailing, and dialing. Some of the exercises are illustrated with hand-drawn diagrams. The Menusration of Heights and Distances section contains color drawings of buildings and trees, and some have been altered with notes in different hands and with humorous additions. For instance, a drawing of a tower was drawn into a figure titled “Egyptian Mummy.” Some of the images are identified: “A rude sketch of the Middlesex canal,” Genl Warren’s monument on Bunker Hill,” “Noddles Island,” “the fields of Elysium,” and the “Roxbury Canal.” The annotations and additional drawings are unattributed.

Student lecture notes of Benjamin Peirce, 1797-1798

This collection contains approximately twenty-three handwritten lecture summaries on six leaves made by Harvard undergraduate Benjamin Peirce between September 1797 and November 22, 1798. The summaries generally provide a few sentences describing the topic covered and primarily pertain to lectures on English grammar delivered by Eliphalet Pearson, the Hollis Professor of Hebrew and other Oriental Languages. There are also summaries for single lectures by David Tappan, the Hollis Professor Divinity; Samuel Webber, the Hollis Professor of Mathematics and Natural Philosophy; and John Snelling Popkin, the Greek Tutor from 1795 to 1798, and later the Eliot Professor of Greek Literature. There is also an undated summary of a lecture by Benjamin Waterhouse, the Hersey Professor of Theory and Practice of Physic.

Grammatica graeca, 1763

Bound volume containing a handwritten Greek grammar compiled by Joseph Drury beginning in 1763. The last sixteen pages contain a historical poem beginning, “Mason might once assert a Poets Claim. / But he must needs write.” The poem contains references to the “Great Patriot P—,“ the Roman conquest of Gall, Caeser, Versailles, and includes the verses, “How the King doth all his Cooks excel / Besides he longs to kiss his P / Saving your presence Louis keeps a whore.”

Letters from Benjamin Vaughan,

Six letters in which Vaughan writes about prominent scientists, artists, and musicians, such as violinist Louis Ostinelli and engraver Jacob Perkins. Other topics include social interactions with Tudor’s sisters Delia Tudor Stewart and Emma Tudor Gardiner, and Emma’s husband, Robert Hallowell Gardiner.

Letter from Charles B. Bullard to William Tudor,

Letter enclosed with two letters to be delivered to Perkins & Co. regarding quicksilver.

Letter from Samuel Willard, Harvard College, Cambridge, Massachusetts, to Catherine Willard, Petersham, Massachusetts, July 14, 1802

Willard explains that he is very busy and discusses the poor health of his brother Ephraim Willard, and asks for more information about his current condition.