751 resultados para Marcador microssatélite


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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Transmissible venereal tumor (TVT) is a neoplasia that develops naturally in dogs. It can be easily transplanted, which demonstrates its ability to spread from animal to animal. Since the Linr-1/c-MYC rearrangement in TVT cells had not been studied at the Veterinary Hospital of the Veterinary School, Unesp in Botucatu, SP, this study aimed to detect this genetic alteration specific to this kind of tumor by means of the polymerase chain reaction (PCR). Twenty dogs with cytological diagnosis of TVT were used. Samples of neoplastic cells were collected to determine the presence of the Line-1/c-MYC marker. The rearrangement characterized by 340bp amplicons did not vary, in agreement with previous studies using the same methodology. This contributed to a more precise identification of persistent tumor cells in cases in which gross or microscopical detection was not possible.

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The Asian rust currently is the main disease of soybean culture, having as characteristics the difficult control, by start at the bottom of plants where penetration of the droplets is harder. The fine droplets has been used with the intention of improve the penetration and increase efficiency of agrochemicals, but that are losses by drift easily. New products have been developed to increase deposition of the drops at targets. The aim of this work was evaluate the TA- 35 capacity to improving the deposition of fungicides spray solution with or without mineral oil by aerial and ground applications. Was used a factorial 3x2, three spray solutions composed by Priori Xtra (concentrated suspension of azoxystrobin 200 g L-1 + cyproconazole 80 g L-1 ) mixed with adjuvants, Nimbus (emulsifiable concentrate containing aliphatic hydrocarbons 428 g L-1 ) and TA-35 (soluble concentrate containing sodium lauryl ether sulfate, surfactants, sequestering agents and emulsifiers), in aerial and ground applications. In ground applications was used 50 L ha-1 , TXA 8002 VS spray nozzles and on aerials was used 15 L ha-1 , Turboaero atomizer, both applying fine droplets. Was utilized the Brilliant Blue (FD & C n. 1) tracer to determine the deposits. There were used glass slides as targets to collect spray droplets. After to extract the tracer of the targets using distilled water, the samples were analyzed by spectrophotometry, thereby was possible quantify the tracer deposited on each glass slide. A study to evaluate possible losses of the tracer by degradation or retention also was done. The comparative analysis of treatments was done by statistical method "Confidence Interval for Differences Between the Averages" with 95% of confidence degree (IC95%). There was degradation or retention of the tracer between the processes of application of the droplets and the processing of the samples. The deposition averages with the presence of TA-35 were greatest for both sprayers however, there were not significant differences among the treatments. The viability of TA-35 use may consider other parameters or complementary studies.

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O objetivo deste trabalho foi avaliar diferentes pontas e taxas de pulverização na deposição de calda na cultura do milho e nas plantas daninhas Euphorbia heterophylla e Brachiaria plantaginea, localizadas nas linhas e nas entrelinhas da cultura. O experimento foi conduzido em delineamento de blocos ao acaso, em esquema fatorial 2 x 2 (2 pontas de pulverização DG 11002 VS (jato plano e gotas médias) e TXVK08 (jato cônico e gotas muito finas) x 2 taxas de aplicação, 100 e 200 L ha-1) com quatro repetições. As aplicações ocorreram aos 13 dias após a germinação do milho (3 a 5 folhas expandidas), as plantas de E. heterophylla apresentavam de 2 a 4 folhas e as plantas de B. plantaginea de 2 a 3 folhas. Utilizou-se como marcador o corante Azul Brilhante (FD&C-1) na concentração de 3000 ppm. Conclui-se que os maiores depósitos médios nas plantas de milho foram proporcionados pela ponta TXVK08, independente da taxa utilizada e que os depósitos mais uniformes ocorreram quando utilizou-se a taxa de 200 L ha-1. Para as plantas daninhas localizadas na linha da cultura, as pontas de pulverização promoveram depósitos de calda mais uniformes na B. plantaginea do que na E. heterophylla, em ambas as taxas de aplicação. Para a B. plantaginea localizadas na entrelinha da cultura a ponta DG 11002VS foi a que proporcionou depósitos de gotas mais uniformes, sendo que para E. heterophylla a ponta mais eficiente em depositar gotas foi a TXVK08.

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Pós-graduação em Biociências - FCLAS

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Pós-graduação em Desenvolvimento Humano e Tecnologias - IBRC

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)