Diálogos culturales entre ayer y hoy : Ecos lucrecianos en la ciencia actual

Teniendo en cuenta que el motivo convocante de estos "diálogos culturales" es la naturaleza, el texto lucreciano insta a interrogarnos sobre los principios y "la naturaleza de las cosas". A partir de la consideración de Duncan Kennedy (2007: 376-377) en cuanto a la significación de la obra de Lucrecio, "haciendo un texto del universo" (Making a Text of the Universe), resulta pertinente analizar las hipótesis que el epicúreo latino expone acerca de la Física atomista. La evocación de los axiomas nullam rem e nilo gigni divinitus umquam (I.150: "ninguna cosa se engendra jamás de la nada por obra divina") y quicque in sua corpora rursum / dissolvat natura neque ad nilum interemat res (I.215-216: "la naturaleza inversamente disuelve cada cosa en sus elementos y no reduce las cosas a la nada") constituye la idea transversal del sistema, que busca contemplar la Naturaleza y obtener un saber surgido de ese acto de pensamiento. Es por ello que, según D. Sedley (2004: 37), el meollo temático del texto reside en el estudio del aspecto y la racionalidad de lo natural, procedimientos especulativos que están comprendidos en el término epicúreo physiología. Este fue el comienzo de un derrotero que, para P. de la Llosa (2000: 10-11), significó "una intuición genial" de los atomistas griegos, completándose más tarde con un segundo impulso a partir de los fundamentos "físicos" de los epicúreos. Lucrecio, sin encajar en el establishment romano, sentó las bases de este labor científico atomista que, eclipsado por varias centurias, se reavivó en el s. XIV para continuar con altibajos hasta el presente. Desde la perspectiva de esta perduración, los objetivos de esta comunicación residen en comprobar la aproximación de los postulados lucrecianos y su veracidad en relación con algunas de las concepciones científicas-físico-químicas-actuales

Le Corbusier (1887–1965)

Le Corbusier was a Swiss architect and urbanist who acquired French nationality in 1930, having set up his studio (“the atelier of patient research”) in that country. Just as he assumed an unconditional continuity in relation to the past, he also clearly confronted the circumstances of his time. Many of his works became icons of Modernism, like the Villa Savoye (1928), the Marseilles Housing Unit (1945), the Ronchamp Chapel (1950), the Convent of Sainte-Marie de la Tourette (1953) and the Chandigarh Capitol Complex (1950-55), to mention just a few examples. His architecture reflected the development of a modern industrialized economy, a western avant-garde society and a vibrant political and social context. He made a mark not only with his constructed work, but also with designs that were never built (and which were progressive in character), his painting (which reflected his experimentalist nature) and with his theoretical texts, which today bear witness to his modernist doctrine. Le Corbusier was above all one of the most prolific thinkers of Modernism, and one of the greatest figures of the 20th century.

Predição de ganhos genéticos via modelos mistos em progênies de café Conilon.

Objetivou-se, neste trabalho, avaliar os ganhos genéticos preditos por meio de diferentes índices de seleção pela metodologia REML/BLUP, em cinco caracteres de interesse ao programa de melhoramento do café conilon do Incaper. Foram avaliadas 8 progênies de meios-irmãos, de ciclo de maturação precoce, média de duas safras, com três repetições, o que totalizou 1368 observações, utilizados os índices de seleção clássico, multiplicativo e com base na soma de postos. Avaliaramse, na época de colheita, as características tamanho dos grãos (TG), produtividade (PRO), porte (PT), vigor vegetativo (VIG) e grau de inclinação (GI). A população foi avaliada na Fazenda Experimental de Marilândia, região Noroeste do estado do Espírito Santo. As análises genético-estatísticas foram realizadas pelo programa Selegen - REM/BLUP. Verificou-se, a partir da análise dos parâmetros genéticos, um excelente potencial seletivo entre famílias, para todas as características avaliadas. O índice Mulamba e Mock foi o que mostrou maior eficiência de seleção entre famílias de meios-irmãos de café conilon.

Sleep disorders in Multiple System Atrophy: a longitudinal study

Introduction: A higher frequency of sleep and breathing disorders in Multiple System Atrophy (MSA) populations is documented in literature. The analysis of disease progression and prognosis in patients with sleep and breathing disorders could shed light on specific neuropathology and pathophysiology of MSA. Objective: To characterize sleep disorders and their longitudinal modifications during disease course in MSA patients, and to determine their prognostic value. Methods: This is a retrospective and prospective cohort study including 182 MSA patients (58.8% males). Type of onset was defined by the first reported motor or autonomic symptom/sign related to MSA. The occurrence of symptoms/signs and milestones of disease progression and their latency were collected. REM sleep behaviour disorder (RBD) and stridor were video-polysomnography (VPSG)-confirmed. VPSG recordings were analysed in a standardized fashion during the disease course. Survival data were based on time to death from the first symptom of disease. Results: Isolated RBD represented the first MSA symptom in 30% of patients, preceding disease onset according to international criteria with a median of 3(1–5) years. Patients developing early stridor or presenting with RBD at disease onset showed a more rapid and severe disease progression. These features had independent negative prognostic value for survival. Sleep architecture was characterized by peculiar features which could represent negative markers in MSA prognosis. Patients with stridor treated with tracheostomy showed a reduced risk of death. Conclusions: This is one of the first studies focusing on longitudinal progression of sleep in MSA. Sleep disorders are key features of disease, playing a role in presentation, prognosis and progression. In our MSA cohort, RBD represented the most frequent mode of disease presentation. Moreover, some specific clinical and instrumental sleep features could represent a hallmark of MSA and could be involved in prognosis and, in particular, in sudden death and death during sleep.

Circadian rhythms, sleep and autonomic function in progressive supranuclear palsy: characteristic features and reciprocal interactions

Background: Progressive supranuclear palsy (PSP) is a rare neurodegenerative condition. The aims of this study were to evaluate the association between sleep, the circadian system and autonomic function in a cohort of PSP patients. Methods: Patients with PSP diagnosed according to consensus criteria were recruited prospectively and retrospectively and performed the following tests: body core temperature (BcT), sleep-wake cycle, systolic and diastolic blood pressure (SBP, DBP) continuous monitoring for 48 h under controlled environmental conditions; cardiovascular reflex tests (CRTs). The analysis of circadian rhythmicity was performed with the single cosinor method. For state-dependent analysis, the mean value of variables in each sleep stage was calculated as well as the difference to the value in wake. Results: PSP patients presented a reduced total duration of night sleep, with frequent and prolonged awakenings. During daytime, patients had very short naps, suggesting a state of profound sleep deprivation across the 24-h. REM sleep behaviour disorder was found in 15%, restless legs syndrome in 46%, periodic limb movements in 52% and obstructive sleep apnea in 54%. BcT presented the expected fall during night-time, however, compared to controls, mean values during day and night were higher. However BcT state-dependent modulation was maintained. Increased BcT could be attributed to an inability to properly reduce sympathetic activity favoured by the sleep deprivation. At CRTs, PSP presented mild cardiovascular adrenergic impairment and preserved cardiovagal function. 14% had non-neurogenic orthostatic hypotension. Only 2 PSP presented the expected BP dipping pattern, possibly as a consequence of sleep disruption. State-dependent analysis showed a partial loss of the state-dependent modulation for SBP. Discussion: This study showed that PSP presented abnormalities of sleep, circadian rhythms and cardiovascular autonomic function that are likely to be closely linked one to another.

Evaluation of alpha-synuclein RT-QuIC for an early and differential diagnosis of Lewy body disease

Synucleinopathies are a group of neurodegenerative diseases characterized by tissue deposition of insoluble aggregates of the protein α-synuclein. Currently, the clinical diagnosis of these diseases, including Parkinson’s disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA), is very challenging, especially at an early disease stage, due to the heterogeneous and often non-specific clinical manifestations. Therefore, identifying specific biomarkers to aid the diagnosis and improve the clinical management of patients with these disorders represents a primary goal in the field. Pursuing this aim, we applied the α-Syn Real-Time Quaking-Induced Conversion (RT-QuIC), an ultrasensitive technique able to detect minute amounts of amyloidogenic proteins, to a large cohort of 953 CSF samples from clinically well-characterized (“clinical” group), or neuropathologically verified (“NP” group) patients with parkinsonism or dementia. Of significance, we also studied patients with prodromal synucleinopathies (“prodromal” group), such as pure autonomic failure (PAF) (n = 28), isolated REM sleep behavior disorder (iRBD) (n = 18), and mild cognitive impairment due to probable Lewy body (LB) disease (MCI-LB) (n = 81). Our findings show that α-syn RT-QuIC can accurately detect α-Syn seeding activity across the whole spectrum of LB-related disorders (LBD), exhibiting a mean sensitivity of 95.2% in the “clinical” and “NP” group, while ranging between 89.3% (PAF) and 100% (RBD) in the “prodromal group”. Moreover, we observed 95.1% sensitivity and 96.6% specificity in the distinction between MCI-LB patients and cognitively unimpaired controls, demonstrating the solid diagnostic potential of α-Syn RT-QuIC in the early phase of the disease. Finally, 13.3% of MCI-AD patients also had a positive test, suggesting an underlying LB co-pathology. This work demonstrated that α-Syn RT-QuIC is an efficient assay for accurate and early diagnosis of LBD, which should be implemented for clinical management and recruitment for clinical trials in memory clinics.

Skin as a promising biomatrix for the early detection of misfolded proteins by RT-QuIC in neurodegenerative diseases

Real-Time Quaking-Induced Conversion (RT-QuIC) is an ultrasensitive assay capable of detecting pathological aggregates of misfolded proteins in biospecimens. In recent years, efforts have been made to find a more feasible and convenient biomatrix as an alternative to CSF, and skin biopsy may be a suitable candidate. This project aimed to evaluate the diagnostic performance of skin RT-QuIC in 3 different cohorts of patients: 1. Creutzfeldt-Jakob disease (CJD), 2. Lewy body disease (LBD), and 3. Isolated REM sleep behavior disorder (iRBD). We studied 71 punch skin samples of 35 patients with CJD, including five assessed in vitam, using 2 two different substrates: Bank vole 23-230 (Bv23-230) and Syrian hamster 23-231 (Ha23-231) recombinant prion protein. Skin prion RT-QuIC showed a 100% specificity with both substrates and a higher sensitivity with the Bv23-230 than Ha23-231 (87.5% vs. 65.6%, respectively). Forty-one patients underwent both lumbar puncture (LB) and skin biopsy; CSF and skin RT-QuIC showed a high level of concordance (38/41, 92.7%). Then, we analyzed samples taken in vitam (n=69) or postmortem (n=49) from patients with Parkinson’s disease (PD), dementia with Lewy bodies (DLB), incidental Lewy body pathology, and neurological controls. Skin α-syn RT-QuIC distinguished LBD patients with an overall accuracy of 94.1% in the two cohorts (sensitivity, 89.2%; specificity, 96.3%). Seventy-nine patients underwent both CSF and skin α-syn RT-QuIC, and the two assays yielded similar diagnostic accuracy (skin, 97.5%; CSF, 98.7%). Finally, we studied 91 iRBD patients and 41 control. In the skin, RT-QuIC showed a sensitivity of 76.9%, specificity of 97.6%, and 82.0% accuracy. 128 participants (88 patients plus 40 controls) underwent both CSF and skin RT-QuIC. The two protocols showed 99.2% of concordance. These works confirmed that skin punch biopsies might represent a valid and convenient alternative to CSF analysis for an early diagnosis of prion diseases and LB-related pathologies.

La usucapion y el registro de la propiedad

En el sistema español, cuando la transmisión jurídico-real trae causa de un contrato, seguimos la teoría del título y el modo, alejándonos así del modelo consensualista adoptado en el Derecho italiano, en el que basta con la mera celebración del título, para que se entienda producida la transmisión. Es decir, que, en nuestro derecho, hasta que no tiene lugar la entrega de la cosa objeto del contrato, no se entiende producida la transmisión. No resulta necesario, en cambio, la inscripción del negocio en el Registro de la Propiedad (inscripción declarativa y no constitutiva). Pero, ¿qué ocurre cuando quien transmite el derecho no es el verdadero titular? Pues que, en estos casos, para tratar de sanar esa falta de titularidad del tradente, entra en juego la usucapión, que es uno de los modos originarios contemplados en la legislación civil española de adquirir la propiedad y otros derechos reales, que se basa en la posesión. Y como la usucapión puede operar al margen del Registro (de inscripción declarativa, como ya hemos dicho), es muy probable que se produzcan desajustes entre lo reflejado por este y la realidad extrarregistral. En el presente trabajo analizamos precisamente la respuesta que la legislación hipotecaria española, a través de los actuales arts. 35 y 36 LH, ha tratado de ofrecer a las distintas cuestiones que se suscitan cuando realidad registral y extrarregistral (o posesoria) entran en conflicto.