Análise da susceptibilidade de leveduras isoladas de insetos sociais aos antifúngicos

A frequência de micoses invasivas causadas por fungos oportunistas, incluindo leveduriformes, vem aumentando significativamente nas últimas décadas e atinge principalmente indivíduos imunocomprometidos. A tribo Attini possui cerca de 230 espécies de formigas e todas possuem o hábito de cultivar e se alimentar de fungos. Algumas espécies cultivam o fungo mutualista em nódulos poligonais sólidos com 0,25 a 0,50mm de diâmetro, aproximadamente. No interior desses nódulos, encontram-se células na forma de leveduras, que quando semeadas em meio de cultura se desenvolvem como fungo filamentoso. Este tipo de fungicultura está restrito a algumas espécies do gênero Cyphomyrmex. Neste trabalho, isolamos 30 leveduras dos nódulos de oito ninhos de Cyphomyrmex transversus da região de Ilhéus (BA), e analisamos a susceptibilidade aos seguintes antifúngicos: Anfotericina B (AMB), Fluconazol (FCZ), Voriconazol (VCZ) e Itraconazol (ITZ). A caracterização das estirpes foi feita através de métodos morfológicos e moleculares, incluindo-se o sequenciamento dos domínios D1/D2 do rDNA. A avaliação da atividade antifúngica foi realizada através do método de microdiluição, de acordo com a norma M27-A2 (CLSI, 2002). Candida parapsilosis ATCC 22019 e Candida krusei ATCC 6258 foram utilizadas como controle. Os resultados foram interpretados segundo os pontos de corte (breakpoints) aplicados ao gênero Candida. As estirpes foram identificadas como Trichosporon asahii (18), T. coremiiforme (4), Candida carpophila (syn. C. guilliermondii) (4), Galactomyces candidum (syn. Geotrichum candidum) (3) e Cryptococcus laurentii (1). Todas as espécies são conhecidas por possuírem representantes que já foram relatados como patógenos oportunistas. Elas foram sensíveis aos azóis FCZ, VCZ e ITZ, com exceção de G. candidus, cujas estirpes apresentaram sensibilidade dose-dependente a FCZ e ITZ... (Resumo completo, clicar acesso eletrônico abaixo)

Estudo da inflexão da Serra do Garrote e sua influência na zona de falha de Vazante (MG)

The Vazante Fault Zone (VFZ), located northwestward of Minas Gerais, host the largest zinc deposit known in the Brazilian territory. This structure is hosted in Vazante’s Group rocks, a metassedimentary sequence of marine environment. Near Vazante is situated the south end of the VFZ. To the west, occur the Serra do Garrote inflexion, characterized by a curvature in the contact of Formations Serra do Garrote and Serra do Poço Verde. This structure is through the analysis of aerial imagery of the region and represented in the published geological maps. The objective of this work is to understand what causes this inflexion and determine whether it affects the VZF, causing a shift in the same, and possibly, in the mineralization as well. To this end, it was done a mapping work in the region covering the Serra do Garrote inflexion and the south end of the VFZ, in 1:25.000 scale, supplemented by petrographic description of thin section and geologic sections, with cooperated to the understanding of the structural evolution of the region. Data analysis allowed the identification of six deformation phases. The D1 an D2 phases generated the main foliation. The D3 phase generate kink bands folds, with NS axis and vertical axial plane. The fourth phase is responsible for generating the Vazante Fault Zone. The fifth phase produces low angle folds and shear zones, subparallel to S1//S2. The last phase generates folds with NW axis and vertical axial plane, with causes the inflection of lithologic contactas. Field observations also make possible the conclusion that the Vazante Fault Zone presents a south continuation, which is affected by deformation associated to D6 phase attributing to the trace of the VFZ a curved geometry, similar to that exhibited by lithologic contacts between units of the map

Aplicação de extratos de algas marinhas em cafeeiro sob deficiência hídrica e estresse salino

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Biometria ocular ultrassonográfica e dopplerfluxometria das artérias oftálmica interna e central da retina em macaco-prego (Sapajus spp)

Pós-graduação em Biotecnologia Animal - FMVZ

Dimensões das furcas de primeiros pré-molares superiores e sua relação com curetas periodontais

The aim of this in vitro study was to comparatively evaluate the mesio and distal furcation entrance measurements of first maxillary premolar. The measurements were compared with different curette blades. A caliper was used by an examinator to acquire the measurements of root trunk (TR); 1 mm (D1) and 2 mm (D2) both below the furcation entrance. For this study Gracey, Mini Gracey, Padua Lima (PL) and Goldman Fox 4 curettes (Millenium) were selected. Measurements of DT - total distance of the active blade length, DI - width of the active blade, DM - width of the medium part of the active blade were obtained for the curettes. The measurements were obtained in both the coronary face and in the lateral face. The data TR, D1 and D2 presented normal distribution and were statistically analyzed by paired t-test. Statistically significant differences were found in the mesial root trunk region (TR) of both premolars. The mean of the measurements was greater than the distal. Mean and standard deviation were obtained, and both Gracey and Mini Gracey showed mean measurements compatible with the closer furcation entrance (D1 - 1 mm). Goldman Fox 4 showed adaptation only in the mesial face, and Padua Lima showed no access to any of the faces. Thereby it is concluded that the access of the furcation is narrow (D1). The mesial face of the root trunk (TR) showed mean measurement greater than distal face. Gracey and Mini Gracey curettes demonstrated to be compatible for both faces, mesial and distal of the first maxillary premolars studied.

Obtenção de anticorpos monoclonais murinos contra antígenos de Staphylococcus aureus resistentes à meticilina por imunização subtrativa

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Uso de variáveis relacionadas com a produção embrionária, em camundongos, como fatores preditivos

Mouse embryo production by superstimulation is a multifactorial process. To minimize the number of sacrificed animals and to maximize the results of the superstimulatory treatment, it should be possible to predict the risk of do not get embryos from such a treated animal. This work aimed to evaluate if the variables - hormone concentration and the timing of its administration, the copulatory plug presence and individual male used to mating – could be predictive factors on the mouse embryo production. Females were distributed in four groups (cross-over design) related to scheduled superstimulation treatment (1300h or 1700h) and eCG/hCG administered concentration (5 or 10IU). After the hCG treatment, females were put to mate. On the next morning it was verified the presence of a copulatory plug (D0.5). Embryo recovery was performed from D2.5 to D4.5 by flushing the oviducts and uterine horns. Total structures recovered (TSR) and the viable embryos (VE) were classified by its morphology. Viability rate (VR) was calculated with VE in relation to TSR (x100). Group comparison was analyzed with 5% of significance. There were no significant differences among groups, even when only main effects were analyzed (hormone concentration and timing of its administration). There was significant difference in VR from animals with or without plug and from the worst and best males used. It was concluded that neither the hormone concentration nor timing of its administration - or their association – was significant as predictive factors for the embryo production. However, the plug presence was related to higher VR.

Análise da relação filogenética entre Triatoma carcavalloi Jurberg, Rocha; Lent, 1998; Triatoma circummaculata Stal, 1859; Triatoma klugi Carcavallo, Jurberg, Lent; Galvão, 2001 e Triatoma rubrovaria Blanchard, 1843 (Hemiptera, Reduviidae) baseada no sequenciamento de genes do DNA mitocondrial e nuclear

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudos das interações do peptídeo antimicrobiano Polybia MP1 com membranas modelo: mecanismo de ação e especificidade

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Bioprospecção de leveduras fermentadoras de xilose visando a produção de etanol a partir de bagaço de cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Two new feather mites of the genus Calcealges Gaud, 1952 (Acari: Trouessartiidae) from antbirds (Passeriformes: Thamnophilidae) in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients

Urothelial bladder carcinoma (UBC) is heterogeneous in its pathology and clinical behaviour. Evaluation of prognostic and predictive biomarkers is necessary, in order to produce personalised treatment options. The present study used immunohistochemistry to evaluate UBC sections containing tumour and non-tumour areas from 76 patients, for the detection of p-mTOR, CD31 and D2-40 (blood and lymphatic vessels identification, respectively). Of the non-tumour and tumour sections, 36 and 20% were scored positive for p-mTOR expression, respectively. Immunoexpression was observed in umbrella cells from non-tumour urothelium, in all cell layers from non-muscle-invasive (NMI) tumours (including expression in superficial cells), and in spots of cells from muscle-invasive (MI) tumours. Positive expression decreased from non-tumour to tumour urothelium, and from pTl/pTis to pT3/pT4 tumours; however, the few pT3/pT4 positive cases had worse survival rates, with 5-year disease-free survival being significantly lower. Angiogenesis occurrence was impaired in pT3/pT4 tumours that did not express p-mTOR. In conclusion, p-mTOR expression in non-tumour umbrella cells is likely a reflection of their metabolic plasticity, and extension to the inner layers of the urothelium in NMI tumours is consistent with an enhanced malignant potential. The expression in cell spots in a few MI tumours and absence of expression in the remaining tumours is intriguing and requires further research. Additional studies regarding the up- and downstream effectors of the mTOR pathway should be conducted.

Variational and perturbative schemes for a spiked harmonic oscillator

A variational analysis of the spiked harmonic oscillator Hamiltonian operator - d2/dx2 + x2 + l(l + 1)/x2 + λ|x| -α, where α is a real positive parameter, is reported in this work. The formalism makes use of the functional space spanned by the solutions of the Schrödinger equation for the linear harmonic oscillator Hamiltonian supplemented by a Dirichlet boundary condition, and a standard procedure for diagonalizing symmetric matrices. The eigenvalues obtained by increasing the dimension of the basis set provide accurate approximations for the ground state energy of the model system, valid for positive and relatively large values of the coupling parameter λ. Additionally, a large coupling perturbative expansion is carried out and the contributions up to fourth-order to the ground state energy are explicitly evaluated. Numerical results are compared for the special case α = 5/2. © 1989 American Institute of Physics.

Myocardial contractility impairment with racemic bupivacaine, non-racemic bupivacaine and ropivacaine. A comparative study

To study racemic bupivacaine, non-racemic bupivacaine and ropivacaine on myocardial contractility. Isolated Wistar papillary muscles were submitted to 50 and 100 mM racemic bupivacaine (B50 and B100), non-racemic bupivacaine (NR50 and NR100) and ropivacaine (R50 and R100) intoxication. Isometric contraction data were obtained in basal condition (0.2 Hz), after increasing the frequency of stimulation to 1.0 Hz and after 5, 10 and 15 min of local anesthetic intoxication. Data were analyzed as relative changes of variation. Developed tension was higher with R100 than B100 at D1 (4.3 ± 41.1 vs -57.9 ± 48.1). Resting tension was altered with B50 (-10.6 ± 23.8 vs -4.7 ± 5.0) and R50 (-14.0 ± 20.5 vs -0.5 ± 7.1) between D1 and D3. Maximum rate of tension development was lower with B100 (-56.6 ± 38.0) than R50 (-6.3 ± 37.9) and R100 (-1.9 ± 37.2) in D1. B50, B100 and NR100 modified the maximum rate of tension decline from D1 through D2. Time to peak tension was changed with NR50 between D1 and D2. Racemic bupivacaine depressed myocardial contractile force more than non-racemic bupivacaine and ropivacaine. Non-racemic and racemic bupivacaine caused myocardial relaxation impairment more than ropivacaine.

Yamadazyma riverae sp. nov., a yeast species isolated from plant materials

Nine strains of a novel yeast species were isolated from rotting wood, tree bark, ant nests or living as endophytes in leaves of Vellozia gigantea. Analysis of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene showed that this species is related to Candida insectorum in the Yamadazyma clade. The new species differs from its closely related species by 10 and 11 substitutions in the ITS region and the D1/D2 domains of the large subunit of the rRNA gene, respectively. The species is heterothallic and forms asci with one to two hat-shaped ascospores. The name Yamadazyma riverae sp. nov. is proposed. The type strain of Yamadazyma riverae sp. nov. is UFMG-CM-Y444T (= CBS 14121) and the allotype strain is TT12 (= CBS 14098 = UFMG-CM-Y577). The Mycobank number is MB 813221.