Avalia����o do banco de disserta����es e teses da associa����o brasileira de antropologia: uma an��lise cienciom��trica

VITULLO, Nadia Aurora Vanti. Avalia����o do banco de disserta����es e teses da Associa����o Brasileira de Antropologia: uma an��lise cienciom��trica. 2001. 143 f. Disserta��ao (Mestrado) - Curso de Mestrado em Biblioteconomia e Ci��ncia da Informa����o, Pontif��cia Universidade Cat��lica de Campinas, Campinas, 2001.

Biblioteca digital de teses e disserta��oes: uma refer��ncia fundamental

OLIVEIRA, Raimundo Muniz de. Biblioteca digital de teses e disserta��oes: uma referencia fundamental. In: CINFORM ENCONTRO NACIONAL DE ENSINO E PESQUISA DA INFORMA��AO,HUMANISMO E DESENVOLVIMENTO CIENTIFICO E TECNOLOGICO,7.,2007,Salvador. Anais...Salvador:UFBA, 2007.Disponivel em:www.cinform.ufba.br>. Acesso em: 27 set. 2007. Acesso em: 27 set. 2010.

Bibblioteca Digital de Teses e Disserta����es: uma estrat��gia de preserva����o digital

CUNHA, Jacqueline de Ara��jo. Biblioteca Digital de Teses e Disserta����es: uma estrat��gia de preserva����o da mem��ria cient��fica. 2009. 141f. Disserta����o (Mestrado)- Programa de P��s-Gradua����o em Ci��ncia da Informa����o. Universidade Federal da Para��ba, Jo��o Pessoa, 2009.

Biodegrada����o de deoxinivalenol por aspergillus oryzae e rhizopus sp.: um estudo bioqu��mico de degrada����o e toxicidade

A contamina����o aleat��ria de alimentos por micotoxinas afeta as condi����es de sanidade das dietas de humanos e animais. Dentre as toxinas f��ngicas, deoxinivalenol (DON) se destaca pela freq��ente contamina����o de produtos agr��colas e alimentos e pela sua resist��ncia a degrada����o pelo emprego de m��todos tradicionais de processamento, o que motiva pol��ticas de controle e a busca por t��cnicas de descontamina����o. A descontamina����o biol��gica empregando processsos fermentativos tem sido apontada como uma alternativa promissora, pois permite degradar micotoxinas atrav��s do sistema enzim��tico microbiano e melhorar caracter��sticas funcionais e sensoriais de mat��rias-primas e insumos aliment��cios. Este trabalho teve por objetivo estudar condi����es e mecanismos de biodegrada����o de deoxinivalenol empregando Aspergillus oryzae e Rhizopus sp. em sistemas fermentativos submersos. Para tanto, foi necess��rio adequar metodologia para rea����o de deriva����o na determina����o cromatogr��fica de DON; estudar o potencial e condi����es de degrada����o via fermenta����o submersa por Aspergillus oryzae e Rhizopus sp.; e avaliar a atividade de oxidoredutases e a citotoxicidade dos extratos fementados. A otimiza����o da metodologia estabeleceu a melhor condi����o para a rea����o de deriva����o com 200 ��L de anidrido trifluoroac��tico e 18 mg de bicarbonato de s��dio, durante 6 minutos a 74 ��C na faixa entre 7 e 21 ��g de DON. A quantifica����o de DON residual no meio fermentado mostrou que as esp��cies f��ngicas Rhizopus sp. e Aspergillus oryzae possuem a capacidade de degradar DON demonstrando ��ndices m��dios de 87,4 e 62,4% respectivamente, principalmente quando o meio submerso foi ��gua est��ril e fermenta����o realizada durante 48 horas. A velocidade m��xima de degrada����o neste intervalo foi de 10,8 e 12,4 ppb/h, observando tamb��m um aumento na atividade espec��fica da enzima peroxidase. Os extratos dos fermentados com A. oryzae e Rhizopus sp. apresentaram efeito de inibi����o de prolifera����o celular (IC50) quando concentrados 10 vezes em 48 e 72 horas respectivamente. Os meios fermentados com Rhizopus sp. apresentaram menor efeito (1,5 vezes) quando comparado com Aspergillus oryzae.

In vitro experimental environments lacking or containing soil disparately affect competition experiments of Aspergillus flavus and co-occurring fungi in maize grains

In vitro experimental environments are used to study interactions between microorganisms, and predict dynamics in natural ecosystems. This study highlights that experimental in vitro environments should be selected to closely match the natural environment of interest during in vitro studies to strengthen extrapolations about aflatoxin production by Aspergillus and competing organisms. Fungal competition and aflatoxin accumulation was studied in soil, cotton wool or tube (water-only) environments, for Aspergillus flavus competition with Penicillium purpurogenum, Fusarium oxysporum or Sarocladium zeae within maize grains. Inoculated grains were incubated in each environment at two temperature regimes (25oC and 30oC). Competition experiments showed interaction between main effects of aflatoxin accumulation and environment at 25oC, but not so at 30oC. However, competition experiments showed fungal populations were always interacting with their environments. Fungal survival differed after the 72-hour incubation in different experimental environments. Whereas, all fungi incubated within the soil environment survived; in the cotton-wool environment, none of the competitors of A. flavus survived at 30 oC. With aflatoxin accumulation, F. oxysporum was the only fungus able to interdict aflatoxin production at both temperatures. This occurred only in the soil environment and fumonisins accumulated instead. Smallholder farmers in developing countries face serious mycotoxin contamination of their grains, and soil is a natural reservoir for the associated fungal propagules, and a drying and storage surface for grains on these farms. Studying fungal dynamics in the soil environment and other environments in vitro can provide insights into aflatoxin accumulation post harvest.

Teses dos Programas de P��s-Gradua����o em Ci��ncia da Informa����o n��veis seis na CAPES em rela����o ��s linhas de pesquisa

Estudo de natureza descritiva que utilizou como instrumento metodol��gico a an��lise documental. O artigo teve como finalidade estudar as teses de doutorado dos Programas de P��s-Gradua����o em Ci��ncia da Informa����o (PPGsCI) n��vel seis na CAPES em rela����o as suas linhas de pesquisa, no tri��nio 2010-2012. Obteve-se como resultado, os PPGsCI da Universidade Federal de Minas Gerais (UFMG) e o da Universidade Estadual Paulista ���J��lio de Mesquita Filho��� (UNESP). O universo de estudo foi composto por 52 teses. Para procedimentos de an��lise tem��tica utilizou-se o Tesauro Brasileiro de Ci��ncia da Informa����o. As tem��ticas identificadas nas teses refletem as caracter��sticas e os objetivos propostos na descri����o das linhas de pesquisa dos dois programas. Palavras-chave: Produ����o cient��fica. Comunica����o cient��fica. Literatura Cinzenta. Tesauros.

A survey on prevalence rate of aflatoxigenic species of Aspergillus and residues of aflatoxins by ELISA method in cold-water cultural fish (rainbow trout) feeds in Tehran and West Azarbayjan provinces-Iran

Aflatoxins are one kind of fungal toxins produced by species of toxigenic Aspergillus (A. flavus and A. parasiticus) and in other words they are secondary metabolites which are considered as one of the threatening factors of food consumer's health. In this research 96 samples of cold-water cultural fish feed, rainbow trout, during the seasons of spring and summer of 2007 (every fifteenth of the month) were randomized (by simple and stratified random) to determine: 1. The prevalence rate of aflatoxigenic species of Aspergillus in stored feed of cold-water cultural fish in West Azarbayjan cultural fish farms in both seasons (spring and summer); 2. The residues of total aflatoxin in stored feed of fish in cultural fish farms of West Azarbayjan in both seasons by ELISA method; and 3. The residues of that toxin in feed produced in aquatic feed factories in Tehran and West Azarbyjan provinces with the same method. In order to study prevalence rate of toxigenic species of Aspergillus, pour-plate culture method by general medium such as Malt Extract Agar (M.E.A.) and Sabouraud-Dextrose Agar (S.D.A.) and by standard No.997 of Iranian Standard Institute were used. The produced colonies were examined microscopically. To determine the aflatoxins residues, ELISA method using Agra-Quant kit of Romer Lab company, were applied. The results of this survey indicated that only 8.3% of the samples were infected by A. flavus. A. parasiticus was not observed. There were no significant differences between the prevalence rate of AFT and seasons/months, either (P<0.05). Evaluating mean of aflatoxin rate showed that the rates of this variable are lower than the tolerance levels designated by the joint FAO/WHO expert committee (The mean of AFT in all data was lower than 11 ppb). Furthermore, mean of total AFT residues rates of stored feed of various cultural center of West Azarbayjan and Tehran factories were comparable in spring and summer, and no significant differences were observed (P<0.05). But there were significant differences between the total aflatoxin rates in the feed of West Azarbayjan factory and spring and summer (P<0.05), and AFT residues in spring (8.6 ppb) were higher than summer (6.1 ppb). Prevalence rates of AFT in Tehran feed factories (9.2 ppb) are higher than W. Azarbayjan (7.4 ppb). In other words, location was considered as a decisive factor in total AFT rates of samples. Moreover, the results indicated that there was significant difference between total aflatoxin rates of feed and cultural centers (P<0.05). The mean of AFT rates in embankment dam cultural fish farms (6.75 ppb) and multi-functions cultural fish farms (6.25 ppb) was higher than individual cultural pond (4.67 ppb). In conclusion, the finally results of this survey indicated that the lower rates of Aspergillus is not effective on the presence of total aflatoxin rates in trout feed. Due to low levels of aflatoxin rates (lower than 20 ppb), the produced feed of cold-cultural fishes, Rainbow Trout, in Tehran and West Azarbayjan provinces, in spring and summer of 2007, were safe and healthy both for fish and their consumers.

Biblioteca Digital de Teses e Disserta����es: grau de utiliza����o pelos mestrandos do curso de Ci��ncia da Informa����o das Universidades P��blicas Brasileiras

A pesquisa aborda o uso das Tecnologias de Informa����o e Comunica����o, que vem revolucionando as atividades e ocasionando muitas mudan��as relacionadas ao acesso e uso de informa����es. O objetivo foi analisar o grau de utiliza����o do conhecimento cient��fico produzido pelos Programas de P��s-Gradua����o das Universidades P��blicas Brasileiras, atrav��s da BDTD, pelos mestrandos dos referidos programas. Nos procedimentos metodol��gicos realizados, procurou-se inicialmente analisar o amplo espectro da popula����o do corpus da pesquisa. Em raz��o da impossibilidade de trabalhar com os Programas de P��s-Gradua����o como um todo, optou-se por fazer um recorte, elegendo os cursos de P��s-Gradua����o em Ci��ncia da Informa����o, vez que estes representam o principal segmento social de interesse da pesquisa. Foi utilizado o m��todo de estudo de usu��rios, onde se optou por adotar o grupo, ���estudos orientados aos usu��rios���, que identifica as necessidades e comportamento de acesso e uso da informa����o. Para coletar os dados, elaborou-se um question��rio semi-estruturado com 25 quest��es, que versavam sobre o uso, dificuldades de acesso e recupera����o da informa����o, bem como a satisfa����o na utiliza����o dessa fonte informacional. Dentre os v��rios resultados obtidos, podemos destacar o fato de que a maioria dos mestrandos (71,8%) s�� teve contato com a BDTD somente no momento em que se encontrava cursando o mestrado e, somente 24,3%, tiveram contato durante a gradua����o. Estes resultados representam um problema, que pode afetar o bom desempenho do projeto BDTD, o qual consiste em disseminar e divulgar a produ����o cient��fica dos Programas de P��s-Gradua����o das Universidades P��blicas Brasileiras para a sua comunidade. Foi observado tamb��m, que os mestrandos oriundos do curso de Biblioteconomia tende a ter contato com a BDTD bem mais cedo do que mestrandos de outros cursos de gradua����o. A fim de minimizar o problema detectado, prop��e-se uma divulga����o mais eficaz na gradua����o atrav��s de dois procedimentos: Primeiro, o docente deve fazer uma divulga����o mais eficaz da BDTD junto aos discentes de todos os cursos de gradua����o; segundo: dever�� ser feita a divulga����o na m��dia eletr��nica, atrav��s da inser����o de ��cones da BDTD, nos portais dos Departamentos dos Cursos de Gradua����o das Universidades P��blicas Brasileiras. Acredita-se que com estes procedimentos seja poss��vel aperfei��oar o uso dessa fonte de informa����o cient��fica.

Bibliotecas digitais de teses e disserta����es brasileiras: paradigma do acesso livre �� informa����o cient��fica

A evolu����o da comunica����o cient��fica ao longo do tempo e o impacto causado pela A evolu����o da comunica����o cient��fica ao longo do tempo e o impacto causado pela A evolu����o da comunica����o cient��fica ao longo do tempo e o impacto causado pela se analisar as contribui����es da Biblioteca Digital de Teses e Disserta����es (BDTD)para a comunica����o cient��fica atual, destacando possibilidades emergentes e desafios a serem superados. Apresenta uma breve evolu����o da comunica����o cient��fica ao longo do tempo; discute os impactos causados pela Internet na comunica����o, disponibiliza����o e acessibilidade de informa����es t��cnico-cient��ficas; e caracteriza BDTD destacando os desafios a serem superados. Dentre eles est��o, a quest��o dos direitos autorais que ocasionam a baixa ades��o dos autores ao projeto piloto do IBICT para disponibilizar teses e disserta����es no meio eletr��nico. Para este desafio, s��o apontadas sugest��es.

Panorama brasileiro das bibliotecas digitais de teses e disserta����es

As bibliotecas de teses e disserta����es t��m um papel fundamental para o desenvolvimento cient��fico e cultural de um pa��s. Nesse sentido, buscou-se, de modo geral, analisar o panorama brasileiro das bibliotecas digitais de teses e disserta����es. Especificamente, objetivou-se caracterizar a sociedade da informa����o enquanto contexto das BDTD; caracterizar os v��rios tipos de bibliotecas surgidos a partir da inser����o das novas tecnologias de informa����o e comunica����o; identificar as diretrizes norteadoras da implanta����o de BDTD; e levantar as BDTD existentes no Brasil. Para tato, al��m de levantamento bibliogr��fico, realizou-se uma pesquisa nos sites de institui����es de ensino superior brasileiras que j�� disp��e de bibliotecas digitais de teses e disserta����es em funcionamento. A an��lise e interpreta����o destes dados nos permite considerar que no Brasil as BDTD encontram-se num est��gio embrion��rio, sendo relevante pesquisar a hist��rica implanta����o e configura����o desse novo modelo de biblioteca.

Effect of commercial starter cultures and native yeasts on ochratoxin a production by aspergillus westerdijkiae and penicillium nordicum in meat products

Processed meat products are of worldwide importance and, because of their intrinsic factors as well as the processing methods, they are highly prone to fungal and mycotoxin contamination. Ochratoxin A (OTA) is the most significant mycotoxin in processed meat products. Penicillium nordicum is considered to be responsible for OTA contamination of meat products, as it is highly adapted to salt and protein-rich matrices and is moderately psycrotrophic. However, another OTA-producing fungus, Aspergillus westerdijkiae, adapted to carbon-rich matrices such as cereals and coffee beans, has been recently associated with high levels of OTA in meat products. Several Lactic Acid Bacteria (LAB) and yeasts have been tested as biocontrol agents against P. nordicum growth and OTA production in meat products, with promising results, but none of the studies have considered A. westerdijkiae. The aim of this work was to evaluate in vitro the effect of a commercial starter culture used in sausage fermentation and four yeasts isolated from dry-cured sausage on these two OTA-producing fungi, both in terms of fungal growth and of OTA production, using different meat-based culture media as model systems. The mechanisms underlying the observed effect were also studied. For this purpose, C. krusei, C. zeylanoides, R. mucilaginosa, R. glutinis, a mix of these yeasts and the starter culture were co-inoculated with P. nordicum and A. westerdijkiae in industrial sausage, traditional sausage, and ham-based media, under conditions of water activity, salt concentration and temperature that mimic real conditions at beginning and end of sausage curing process. Fungal growth was determined by measuring colony diameter, and OTA production was quantified by HPLC-FLD after extraction with methanol. Yeasts where found to inhibit significantly the growth of both fungi. P. nordicum was unable to produce detectable OTA in both sausage-based media under any condition. In ham, yeasts reduced OTA production, while the starter culture significantly increased it. Unexpectedly, OTA production by A. westerdijkiae was significantly stimulated in all media tested by all microorganisms. Matrix has a significant effect on OTA production by P. nordicum, but not by A. westerdijkiae, for which only temperature showed to have effect. By testing the mechanisms of action by which starter culture and C. zeylanoides influenced fungal responses, we were able to determine that direct contact and simultaneous growth of test organisms were the mechanisms more significantly involved in the responses. In conclusion, ochratoxigenic fungi do not all respond to antagonistic microorganisms in the same way. The use of biocontrol agents with the intent of reducing fungal growth and mycotoxin production by one fungus can have unexpected effects on others, thus leading to unforeseen safety problems. Further experiments are recommended to properly understand the reasons behind the different effects of microorganisms, to ensure their safe as biocontrol agents.

Antifungal activity of extracts from Atacama Desert fungi against Paracoccidioides brasiliensis and identification of Aspergillus felis as a promising source of natural bioactive compounds

Fungi of the genus Paracoccidioides are responsible for paracoccidioidomycosis. The occurrence of drug toxicity and relapse in this disease justify the development of new antifungal agents. Compounds extracted from fungal extract have showing antifungal activity. Extracts of 78 fungi isolated from rocks of the Atacama Desert were tested in a microdilution assay against Paracoccidioides brasiliensis Pb18. Approximately 18% (5) of the extracts showed minimum inhibitory concentration (MIC) values ��� 125.0 ��g/mL. Among these, extract from the fungus UFMGCB 8030 demonstrated the best results, with an MIC of 15.6 ��g/mL. This isolate was identified as Aspergillus felis (by macro and micromorphologies, and internal transcribed spacer, ��-tubulin, and ribosomal polymerase II gene analyses) and was grown in five different culture media and extracted with various solvents to optimise its antifungal activity. Potato dextrose agar culture and dichloromethane extraction resulted in an MIC of 1.9 ��g/mL against P. brasiliensis and did not show cytotoxicity at the concentrations tested in normal mammalian cell (Vero). This extract was subjected to bioassay-guided fractionation using analytical C18RP-high-performance liquid chromatography (HPLC) and an antifungal assay using P. brasiliensis. Analysis of the active fractions by HPLC-high resolution mass spectrometry allowed us to identify the antifungal agents present in the A. felis extracts cytochalasins. These results reveal the potential of A. felis as a producer of bioactive compounds with antifungal activity.

O problema religioso como pre��mbulo do idealismo italiano : das teses de Maurizio Viroli �� religi��o da liberdade em Croce

O objetivo do presente artigo �� analisar o quanto o chamado idealismo italiano de Croce e Gentile ��, em verdade, dependente da concep����o religiosa italiana. Para tanto, nos deteremos, na primeira parte do nosso trabalho, principalmente numa an��lise das teses de Maurizio Viroli. J�� no segundo parte do nosso artigo nos deteremos na an��lise dessa tem��tica dentro do conceito de religi��o da liberdade em Croce. Assim, ap��s tal invent��rio, teceremos nossas conclus��es. _______________________________________________________________________________ ABSTRACT

Beitr��ge zur kenntniss der pilzgattung Aspergillus ...

Inaug.-diss.--Strassburg.