994 resultados para (de)palatalization patterns, 15th–16th century Portuguese
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Este artigo apresenta uma pesquisa sobre a representação do discurso ficcional embasado na gramática sistêmico - funcional proposta por Halliday e na Lingüística de Corpus, utilizando-se o software WordSmith Tools. A análise focaliza a metafunção ideacional, realizada pelo sistema de transitividade, focalizando os processos mentais e a relação lógico - semântica da projeção. O objetivo da pesquisa foi observar como os pensamentos das personagens de um corpus ficcional são representados através dos verbos de elocução THINK e PENSAR, buscando descrever padrões textuais nos três romances que compõem o corpus.
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Dissertação de Mestrado em Tradução e Assessoria Linguística.
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In the past few years the interest in coagulase-negative staphylococci (CoNS) has significantly increased in human medicine. CoNS are common commensal colonisers of the human skin, although now also recognised as major nosocomial pathogens. Over the last decades, several studies have been carried out in order to understand the pathogenicity mechanisms of CoNS. The well known determinants in the pathogenesis of CoNS infections are their ability to form biofilms and an exceptional resistance to several antibiotics. Nevertheless, there is a lack of studies regarding the commensal lifestyle of these microorganisms. Additionally, it is now hypothesised that commensal bacteria might be a reservoir of pathogenic determinants. Therefore, the work described throughout this thesis was aimed to perform a phenotypic and genotypic characterisation of different CoNS species isolated from healthy Portuguese individuals. A total of 61 CoNS isolates, comprising 7 different species, were obtained and characterised at the level of biofilm formation and antibiotic susceptibility profiles. According to the results, biofilm formation ability and presence of biofilm-associated genes were commonly found features, highlighting their pivotal role in the colonising lifestyle of CoNS. This study also addressed the correlation between phenotypic and genotypic characteristics of biofilm formation, corroborating and raising questions about the importance of some genes in this process. Moreover, it was observed a great proportion of isolates with decreased susceptibility and multiple resistances to some important antibiotics. A significant association between antibiotic resistance and biofilm formation was also demonstrated, and some hypotheses about the nature of such association were provided. Lastly, the expression patterns of two biofilm-associated genes at two distinct biofilm developmental stages were determined, confirming their importance in the accumulative stage of biofilm formation. Overall, the results presented in this thesis indicate that staphylococcal skin flora might be an important reservoir of potentially pathogenic bacteria and, simultaneously, bring to light new perceptions about the molecular basis of staphylococcal biofilm formation, and the nature of the association between antibiotic resistance and biofilm formation.
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ECER 2014 "The Past, the Present and Future of Educational Research in Europe" will take place at the University of Porto from 1 - 5 September 2014.
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Dissertação de Mestrado, Geologia do Ambiente e Sociedade, 8 de Maio de 2015, Universidade dos Açores.
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The handling of waste and compost that occurs frequently in composting plants (compost turning, shredding, and screening) has been shown to be responsible for the release of dust and air borne microorganisms and their compounds in the air. Thermophilic fungi, such as A. fumigatus, have been reported and this kind of contamination in composting facilities has been associated with increased respiratory symptoms among compost workers. This study intended to characterize fungal contamination in a totally indoor composting plant located in Portugal. Besides conventional methods, molecular biology was also applied to overcome eventual limitations.
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Hazing practices in higher education institutions are perceived controversially. This study looked at the perception towards hazing practices of students from the University of the Azores in first cycle (n=247). It analyses if their perception was positive, emphasizing the integrative aspects of hazing practices, or if it was negative, inclining to define those practises like being violent and humiliating. The applied questionnaire measures 3 factors to define individual’s perception with hazing practices (positive relation with hazing practices, negative relation and the social dimension of the practices). This study used descriptive analyses to observe the level of agreement with hazing practices and non-parametrical statistical test to analyse the relationship between their levels of agreement with hazing practices and socio-demographic variables. Results emphasised balanced proportion of students perceiving positively and negatively those practices and incline to define hazing practices with the same components in both perceptions. It didnot highlight a strong mandatory integrative component in students’ perception. Also, results stressed a large part of students disinterested in hazing practices. Additionally factors of hazing practices’ perception showed significant relationships with respondent’s sex, age and academic degree. Hazing practices in this study did not seem to meet their main objectives of welcoming and integrating freshmen in higher education institutions.
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The presence of filamentous fungi was detected in wastewater and air collected at wastewater treatment plants (WWTP) from several European countries. The aim of the present study was to assess fungal contamination in two WWTP operating in Lisbon. In addition, particulate matter (PM) contamination data was analyzed. To apply conventional methods, air samples from the two plants were collected through impaction using an air sampler with a velocity air rate of 140 L/min. Surfaces samples were collected by swabbing the surfaces of the same indoor sites. All collected samples were incubated at 27°C for 5 to 7 d. After lab processing and incubation of collected samples, quantitative and qualitative results were obtained with identification of the isolated fungal species. For molecular methods, air samples of 250 L were also collected using the impinger method at 300 L/min airflow rate. Samples were collected into 10 ml sterile phosphate-buffered saline with 0.05% Triton X-100, and the collection liquid was subsequently used for DNA extraction. Molecular identification of Aspergillus fumigatus and Stachybotrys chartarum was achieved by real-time polymerase chain reaction (RT-PCR) using the Rotor-Gene 6000 qPCR Detection System (Corbett). Assessment of PM was also conducted with portable direct-reading equipment (Lighthouse, model 3016 IAQ). Particles concentration measurement was performed at five different sizes: PM0.5, PM1, PM2.5, PM5, and PM10. Sixteen different fungal species were detected in indoor air in a total of 5400 isolates in both plants. Penicillium sp. was the most frequently isolated fungal genus (58.9%), followed by Aspergillus sp. (21.2%) and Acremonium sp. (8.2%), in the total underground area. In a partially underground plant, Penicillium sp. (39.5%) was also the most frequently isolated, also followed by Aspergillus sp. (38.7%) and Acremonium sp. (9.7%). Using RT-PCR, only A. fumigatus was detected in air samples collected, and only from partial underground plant. Stachybotrys chartarum was not detected in any of the samples analyzed. The distribution of particle sizes showed the same tendency in both plants; however, the partially underground plant presented higher levels of contamination, except for PM2.5. Fungal contamination assessment is crucial to evaluating the potential health risks to exposed workers in these settings. In order to achieve an evaluation of potential health risks to exposed workers, it is essential to combine conventional and molecular methods for fungal detection. Protective measures to minimize worker exposure to fungi need to be adopted since wastewater is the predominant internal fungal source in this setting.
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Individuals spend 80-90% of their day indoors and elderly subjects are likely to spend even a greater amount of time indoors. Thus, indoor air pollutants such as bioaerosols may exert a significant impact on this age group. The aim of this study was to characterize fungal contamination within Portuguese elderly care centers. Fungi were measured using conventional as well as molecular methods in bedrooms, living rooms, canteens, storage areas, and outdoors. Bioaerosols were evaluated before and after the microenvironments' occupancy in order to understand the role played by occupancy in fungal contamination. Fungal load results varied from 32 colony-forming units CFU m(-3) in bedrooms to 228 CFU m(-3) in storage areas. Penicillium sp. was the most frequently isolated (38.1%), followed by Aspergillus sp. (16.3%) and Chrysonilia sp. (4.2%). With respect to Aspergillus genus, three different fungal species in indoor air were detected, with A. candidus (62.5%) the most prevalent. On surfaces, 40 different fungal species were isolated and the most frequent was Penicillium sp. (22.2%), followed by Aspergillus sp. (17.3%). Real-time polymerase chain reaction did not detect the presence of A. fumigatus complex. Species from Penicillium and Aspergillus genera were the most abundant in air and surfaces. The species A. fumigatus was present in 12.5% of all indoor microenvironments assessed. The living room was the indoor microenvironment with lowest fungal concentration and the storage area was highest.
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Tese apresentada para o cumprimento dos requisitos necessários à obtenção do grau de Doutor no ramo de Ciências Musicais
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Apesar da pluriculturalidade que caracteriza algumas regiões de Portugal, existe um desconhecimento e por vezes resistência, em particular das comunidades mais tradicionais e das gerações mais antigas, na aceitação dos imigrantes que escolhem o nosso País como destino de acolhimento. Conhecer as comunidades imigrantes pode ajudar a perceber e a minimizar as dificuldades do convívio pluricultural. Este será, na atualidade, um dos papéis que o animador sociocultural será chamado a desempenhar. Para isso, é necessário que reconheça os recursos legais, metodológicos e teóricos que tem ao seu dispor, sendo estas as premissas que norteiam o presente exercício. Esta análise nasceu de uma proposta de trabalho da Unidade Curricular de Multiculturalidade e Cidadania, mas rapidamente se revelou um importante contributo para o conhecimento da diversidade cultural no nosso país. Após uma contextualização teórica relativa aos principais fluxos migratórios em Portugal, é apresentada a análise dos dados relativos à composição das origens dos imigrantes em Portugal na atualidade e a sua distribuição pelo território português. Este é o ponto de partidapara a reflexão que se lhe seguiu, que visa, por um lado, identificar algumas das problemáticas que resultam dos contextos de diversidade cultural e, por outro, conhecer as propostas do Estado para promover a integração do “outro”. Estes pilares sustentaram a abordagem final relativa ao papel da Animação Sociocultural (ASC) e do animador neste contexto.
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A dissertação de doutoramento visa estudar a situação da Heráldica do Exército na República Portuguesa ao longo do século XX. Consideram-se ainda como objectivos deste estudo aportar novos subsídios para a história geral da Heráldica no século XX em Portugal, que permanece parcialmente inédita e que em certos ramos está por fazer, ao mesmo tempo que se identificou sistemática e tão exaustivamente quanto possível, a produção de Heráldica por parte deste ramo das Forças Armadas, sendo ainda feito uma análise estética das armas produzidas.
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Com a generalização das tecnologias de informação e comunicação na área da saúde, a monitorização remota de pacientes, a partir de dispositivos móveis, é uma realidade que contribui para uma melhor qualidade de vida dos pacientes. Mas, não são só os pacientes que ganham com a introdução destas tecnologias, também os profissionais de saúde e os hospitais retiram as suas vantagens. Os profissionais de saúde são munidos de uma ferramenta móvel de monitorização permanente de sinais vitais, aumentando desta forma a ligação médico-paciente. Relativamente aos hospitais, estes vêem os custos de manutenção reduzirem, em virtude da possibilidade dos pacientes puderem ser monitorizados a partir dos seus lares. Neste projecto pretende-se identificar mecanismos que possibilitem responder de forma eficaz à necessidade de partilha de informação, nomeadamente protocolos de comunicação, segurança e sistemas de integração. Foi projectado um protótipo, constituído por um middleware e uma aplicação cliente móvel, onde o middleware tem como missão garantir a interoperabilidade entre um servidor HL7 e a aplicação cliente, utilizada pelo profissional de saúde. A normalização da informação médica trocada entre o servidor HL7 e o middleware obedece à norma internacional HL7.
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Purpose – Quantitative instruments to assess patient safety culture have been developed recently and a few review articles have been published. Measuring safety culture enables healthcare managers and staff to improve safety behaviours and outcomes for patients and staff. The study aims to determine the AHRQ Hospital Survey on Patient Safety Culture (HSPSC) Portuguese version's validity and reliability. Design/methodology/approach – A missing-value analysis and item analysis was performed to identify problematic items. Reliability analysis, inter-item correlations and inter-scale correlations were done to check internal consistency, composite scores. Inter-correlations were examined to assess construct validity. A confirmatory factor analysis was performed to investigate the observed data's fit to the dimensional structure proposed in the AHRQ HSPSC Portuguese version. To analyse differences between hospitals concerning composites scores, an ANOVA analysis and multiple comparisons were done. Findings – Eight of 12 dimensions had Cronbach's alphas higher than 0.7. The instrument as a whole achieved a high Cronbach's alpha (0.91). Inter-correlations showed that there is no dimension with redundant items, however dimension 10 increased its internal consistency when one item is removed. Originality/value – This study is the first to evaluate an American patient safety culture survey using Portuguese data. The survey has satisfactory reliability and construct validity.
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Dissertação para obtenção do grau de Mestre em Engenharia Civil na Área de Especialização de Edificações