Die kriegsziele der französischen bourgeoisie in Mitteleuropa : mit 4 Karten.

"Literaturverzeichnis": p. 65-67.

Entwicklungsgeschichte und Verwandtschaftsbeziehungen der Hydrozoen.

Mode of access: Internet.

Untersuchungen über den Übergang von Infektionserregern (Milzbrand-, Rotlauf- und Tuberkelbazillen) von der trächtigen Mutter auf den Fötus.

Inaug.-diss. - Hannover, 1912.

Die brücke; gedichte ...

Mode of access: Internet.

Synopsis juris-feudalis, cum semicenturia quæstionum illustrium ex aurea bulla imperatoris Caroli IV, in academia Lipsiensi decem disputationibus publice proposita /

Mode of access: Internet.

Medicinisches aus der altfranzösischen dichtung,

"Verzeichnis der benutzten quellen und ihrer abkürzungen": p. [144]-147.

Geographisch-geologische studien aus dem Böhmerwalde. Die spuren alter gletscher, die seen und thäler des Böhmerwaldes.

With this is bound: Schlagintweit, Robert von. Die pacifischen eisenbahnen in Nordamerika; Berndt, Gustav. Der Alpenföhn in seinem einfluss auf natur- und menschenleben; Supan, Alexander. Archiv für wirthschaftsbeographie; Radde, gustav. Aus den Dagestanischen hochalpen.

Self-condensation of a thiazole-peptide bearing a 21-membered loop into a library of giant macrocycles with multiple orthogonal loops

Tetrapeptide analogue H-[Glu-Ser-Lys(Thz)]-OH, containing a turn-inducing thiazole constraint, was used as a template to produce a 21-membered structurally characterized loop by linking Glu and Lys side chains with a Val-Ile dipeptide. This template was oligomerized in one pot to a library (cyclo-[1](n), n = 2-10) of giant symmetrical macrocycles (up to 120-membered rings), fused to 2-10 appended loops that were carried intact through multiple oligomerization (chain extension) and cyclization (chain terminating) reactions of the template. A three-dimensional solution structure for cyclo-[1](3) shows all three appended loops projecting from the same face of the macrocycle. This is a promising approach to separating pepticle motifs over large distances.

Sequence variation in the Newcastle disease virus genome

Full-length genome sequences of five virulent and five avirulent strains of Newcastle disease virus isolated between 1998 and 2002 in Victoria and New South Wales, Australia were determined. Comparisons between these strains revealed that coding sequence variability in the haemagglutinin-neuraminidase (HN), matrix (M) and phosphoprotein (P) gene sequences appeared to be more variable than in the fusion (F), nucleocapsid (N) and RNA dependent-RNA replicase (L) genes. Sequence analysis of a number of other isolates made during the recent virulent NDV outbreaks, also identified the presence of a number of variants with altered F gene cleavage sites, which resulted in altered biological properties of those viruses. Quasispecies analysis of a number of field isolates indicated the presence of virulent virus in one particular isolate. Gene sequence analysis of the progenitor virus isolated in 1998 showed very little sequence variation when compared to that of a progenitor-like virus isolated in 2001 demonstrating that in the field. viral genome sequence variation appears to be biologically restricted to that of a consensus sequence. (c) 2005 Elsevier B.V. All rights reserved.

Deduced amino acid sequences surrounding the fusion glycoprotein cleavage site and of the carboxyl-terminus of haemagglutinin-neuraminidase protein of the avirulent thermostable vaccine strain I-2 of Newcastle disease virus

A single-tube RT-PCR technique generated a 387 bp or 300 bp cDNA amplicon covering the F-0 cleavage site or the carboxyl (C)-terminus of the HN gene, respectively, of Newcastle disease virus (NDV) strain 1-2. Sequence analysis was used to deduce the amino acid sequences of the cleavage site of F protein and the C-terminus of HN protein, which were then compared with sequences for other NDV strains. The cleavage site of NDV strain 1-2 had a sequence Motif of (112)RKQGRLIG(119), consistent with an avirulent phenotype. Nucleotide sequencing and deduction of amino acids at the C-terminus of HN revealed that strain 1-2 had a 7-amino-acid extension (VEILKDGVREARSSR). This differs from the virulent viruses that caused outbreaks of Newcastle disease in Australia in the 1930s and 1990s, which have HN extensions of 0 and 9 amino acids, respectively. Amino acid sequence analyses of the F and HN genes of strain 1-2 confirmed its avirulent nature and its Australian origin.

Genorne sequence of the thermostable Newcastle disease virus (strain I-2) reveals a possible phenotypic locus

The complete genome sequence of the Australian 1-2 heat-tolerant Newcastle disease virus (NDV) vaccine (master seed stocks) was determined and compared to the sequence of the parent virus from which it had been derived after exposure of the parent stock at 56 degrees C for 30 min. Nucleotide changes were observed at a number of positions with synonymous mutations being greater than those observed for non-synonymous mutations. Sequence data for the HN gene of a parental culture of V4 and two heat-tolerant variants of V4 were obtained. These were compared with the data for the 1-2 viruses and with published sequences for parental V4 and for a number of ND vaccine strains. Sequence analyses did not reveal the ARG 303 deletion in the HN protein, previously claimed to be responsible for the thermostable phenotype. No consistent changes were detected that would indicate involvement of the HN protein in heat resistance. The majority of alterations were observed in the L protein of the virus and it is proposed that these alterations were responsible for the heat-tolerant phenotype of the 1-2 NDV vaccine. (c) 2005 Elsevier B.V. All rights reserved.

A INFLUÊNCIA DAS MULHERES CLÂNICAS NO PENSAMENTO PROFÉTICO DO PÓS-EXÍLIO. UM ESTUDO DE ISAÍAS 57,1-21.

Neste trabalho sobre A Influência das Mulheres Clânicas no Pensamento Profético do Pós-Exílio. Um Estudo de Isaías 57,1-21, propomos apresentar uma pesquisa para demonstrar fundamentalmente quem eram os três grupos de mulheres clânicas, que surgem no Isaías 57,3-9, a saber: hn+n>[o(agoureira),aEßnm. (adultério, significando adúltera - tp,a(nm.) e hnAz(prostituta). E daí desenvolver que influência tiveram na profecia, no período do pós-exílio. Para tal tarefa utilizamos dois métodos: o primeiro, um método diacrônico no qual o texto demonstrou uma visão muito negativa dessas mulheres, já que o pano de fundo onde estaria estabelecido o texto é de forte influência patriarcal. Mas, ao aplicarmos um segundo, o método sincrônico e intertextual, o resultado se mostrou diferente, pois o conjunto de textos onde está incluso, a saber: Isaías 56,1-12; 58,1-14 e 61,1-11, demonstram um programa inclusivo. Assim, no Isaías 56,3-4 - rkªNEh;-!B, (filho do estrangeiro) e syrIêSh; (os eunucos), são admitidos na comunidade; no Isaías 58, 1 bqoß[]y: tybeîl.W (e para casa de Jacó), essa casa representada por um grupo de homens é repreendida por causa do jejum; e no Isaías 61,5-6 ~yrIêz (estranhos) e rkênE ynEåb.W (e filhos de estrangeiro), serão os que alimentarão a comunidade. Devido a isto, surgiu uma hipótese de que uma visão negativa sobre elas não poderia ser aceita dentro de um projeto inclusivo. No entanto a questão deve ser respondida. Partirmos para fazer um mapeamento do modo de vida clânico no Gênesis, um conjunto de textos que fala principalmente da família/clã. Ao estudarmos algumas mães míticas: Eva, Sara, Agar, filhas de Ló e Tamar, e ao compará-las com as de Isaías 57,3-9, muitas das características se mostram semelhantes. Pudemos assim perceber que todas essas mulheres clânicas por possuírem conhecimentos do reino animal e vegetal, exerceram influência na vida e morte das famílias/clãs, assim elas tiveram que serem combatidas pelos grupos de homens ao longo do tempo. Ainda outra característica importante no Pós-Exílio, é a movimentação que as famìlias/clãs realizam, mas, essa ‗saìda é sempre carregada de abundância de fertilidade e resolução de conflito pela solidariedade. Devemos estar na profecia, já que ao cristalizar-se um texto ‗desfavorável contra um grupo de mulheres, na verdade se está denunciando uma violência contra elas.

Characterization of FITC-conjugated lectin binding to Candida albicans

Avidity of yeast and hyphal forms of Candida albicans for FITC-conjugated lectins was determined by flow cytometry and digital microscopy. Yeast phase cells bound Con A, a lectin with marked affinity for mannose, irrespective of growth phase, yet demonstrated little avidity for WGA and SBA. Yeast phase cell avidity for mannose-specific lectins was characterized through determination of FITC-conjugated Con A, LcH, PSA and GNA binding and subsequent calculation of Bmax, EC50 and Hn values. Such an approach, through comparison among FITC-conjugated lectins of differing specific activities, furnishes further insight into exposed outer cell wall mannose moieties. The rank order of lectin affinity as defined by EC50 values was GNA > Con A > LcH > PSA. Values for Hn suggest that lectins predominantly bind to a single receptor class, the relative abundance of which as defined by Bmax values was PSA > GNA > Con A > LcH. Hyphal surfaces in common with yeast phase cells demonstrated marked avidity for FITC-Con A, however, fluorescence of Candida morphological forms differed significantly, indicative of varying outer cell wall mannose exposure.

FITC-lectin avidity of Cryptococcus neoformans cell wall and capsular components

Flow cytometry and confocal microscopy were used to quantify and visualize FITC-lectin binding to cell-surface carbohydrate ligands of log and stationary phase acapsular and capsular Cryptococcus neoformans strains. Cell populations demonstrated marked avidity for terminal a-linked mannose and glucose specific FITC-Con A, mannose specific FITC-GNL, as well as N-acetylglucosamine specific FITC-WGA. Exposure to other FITC-lectins specific for mannose, fucose and N-acetylgalactosamine resulted in little cell-surface fluorescence. The nature of cell-surface carbohydrates was investigated further by measurement of the fluorescence from surfaces of log and stationary phase cell populations after exposing them to increasing concentrations of FITC-Con A and FITC-WGA. Cell fluorescence increased significantly with small increases in FITC-Con A and FITC-WGA concentrations attaining reproducible maxima. Measurements of this nature supported calculation of the lectin binding determinants EC 50, Hn, Fmax and relative Bmax values. EC50 values indicated that the yeast-cell surfaces had greatest affinity for FITC-WGA, however, relative Bmax values indicated that greater numbers of Con A binding sites were present on these same cell surfaces. Hn values suggested a co-operative lectin-carbohydrate ligand interaction. Imaging of FITC-Con A and FITC-WGA cell-surface fluorescence by confocal microscopy demonstrated marked localization of both lectins to cell surfaces associated with cell division and maturation, indicative of dynamic carbohydrate ligand exposure and masking. Some fluorescence was associated with entrapment of FITC-Con A by capsular components, but FITC-Con A and FITC-WGA readily penetrated the capsule matrix to bind to the same cell surfaces labelled in acapsular cells.

X-ray crystallographic and theoretical studies of an anticonvulsant enaminone:methyl 4-(4'-bromophenyl)amino-6-methyl-2-oxocyclohex-3-en-1-oate

Objective: The aims of this study were to establish the structure of the potent anticonvulsant enaminone methyl 4-(4′-bromophenyl)amino-6-methyl-2- oxocyclohex-3-en-1-oate (E139), and to determine the energetically preferred conformation of the molecule, which is responsible for the biological activity. Materials and Methods: The structure of the molecule was determined by X-ray crystallography. Theoretical ab initio calculations with different basis sets were used to compare the energies of the different enantiomers and to other structurally related compounds. Results: The X-ray crystal structure revealed two independent molecules of E139, both with absolute configuration C11(S), C12(R), and their inverse. Ab initio calculations with the 6-31G, 3-21G and STO-3G basis sets confirmed that the C11(S), C12(R) enantiomer with both substituents equatorial had the lowest energy. Compared to relevant crystal structures, the geometry of the theoretical structures shows a longer C-N and shorter C=O distance with more cyclohexene ring puckering in the isolated molecule. Conclusion: Based on a pharmacophoric model it is suggested that the enaminone system HN-C=C-C=O and the 4-bromophenyl group in E139 are necessary to confer anticonvulsant property that could lead to the design of new and improved anticonvulsant agents. Copyright © 2003 S. Karger AG, Basel.