CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere

Inheritance of each chromosome depends upon its centromere. A histone H3 variant, centromere protein A (CENP-A), is essential for epigenetically marking centromere location. We find that CENP-A is quantitatively retained at the centromere upon which it is initially assembled. CENP-C binds to CENP-A nucleosomes and is a prime candidate to stabilize centromeric chromatin. Using purified components, we find that CENP-C reshapes the octameric histone core of CENP-A nucleosomes, rigidifies both surface and internal nucleosome structure, and modulates terminal DNA to match the loose wrap that is found on native CENP-A nucleosomes at functional human centromeres. Thus, CENP-C affects nucleosome shape and dynamics in a manner analogous to allosteric regulation of enzymes. CENP-C depletion leads to rapid removal of CENP-A from centromeres, indicating their collaboration in maintaining centromere identity.

The Iris : an illuminated souvenir for MDCCCLII /

Plates lithographed by Peter S. Duval after drawings by Capt. Seth Eastman.

Album of solo pieces for the harp. [music] /

V. 1. Prelude / W. Loukine. The music-box / Franz Poenitz. Prayer ; Will-o'-the-wisp / A. Hasselmans. Slumber-song / Gabriel Fauré. Marguerite at the spinning-wheel. Polonaise / Margaret Hoberg -- v. 2. Minuet / L. van Beethoven. Serenade / Christian Sinding. Aeolian harp ; The brook / A. Hasselmans. Pattuglia Spagnuola / L.M. Tedeschi. Romance / A. Zabel. Harp solo from the opera Lucia di Lammermoor / Gaetano Donizetti ; arranged by Albert Zabel.

A philosophical grammar of the English language : adapted equally to the use of schools or private study : in which are contained, in numerous instances, theoretical and practical refutations of the most prevailing systems in modern use /

"Recommendations": 10 p. at beginning of vol.

Cotton fiber and processing test results.

Description based on: Crop of 1959 (Aug. 3, 1959).

Illustrated technical dictionary in six languages, English, German, French, Russian, Italian, Spanish /

Vols. 7-13 have added t.p.: Schlomann-Oldenbourgh illustrierte technische wörterbücher, unter mitwirkung hervorragender fachieute des in- und auslandes, hrsg. von Alfred Schlomann ... Müchen und Berlin, Druck und verlag von R. Oldenbourg; London, Constable & Co., etc.

Memoirs of missionary priests and other Catholics of both sexes : that have suffered death in England on religious accounts from the year of our Lord, 1577 to 1684 /

Mode of access: Internet.

The Mason Machine Works, Taunton, Massachusetts, U.S.A., inventors and builders of cotton machinery. Founded 1842. Incorporated 1873. [Catalogue.

Mode of access: Internet.

The Broadway

Plymouth at Broadway 761-4280, party goods - candles - books - candy - gifts. Gift wrapping - wrap and mail service - Autotyping - bulk mailing - Bigby ice-skate exchange.

Narrative of the life of Frederick Douglass, an American slave. /

"Letter from Wendell Phillips, Esq.": pages [xiii]-xvi.

Characterization of the Structure and Dynamics of Biomimetic Peptides by Solid-State NMR

Thesis (Ph.D.)--University of Washington, 2016-05

Investigating the Effect of Histone H4 Sumoylation on Chromatin Structure and Function

Thesis (Ph.D.)--University of Washington, 2016-06

Immobilization of aluminum chloride on MCM-41 as a new catalyst system for liquid-phase isopropylation of naphthalene

A great deal of effort has been made at searching for alternative catalysts to replace conventional Lewis acid catalyst aluminum trichloride (AlCl3). In this paper, immobilization of AlCl3 on mesoporous MCM-41 silica with and without modification was carried out. The catalytic properties of the immobilized catalyst systems for liquid-phase isopropylation of naphthalene were studied and compared with those of H/MCM-41 and H/mordenite. The structures of the surface-immobilized aluminum chloride catalysts were studied and identified by using solid-state magic angle spinning nuclear magnetic resonance (MAS NMR), Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), nitrogen adsorption, and X-ray diffraction (XRD) techniques. The catalytic activity of the immobilized catalysts was found to be similar to that of acidic mordenite zeolite. A significant enhancement in the selectivity of 2,6-diisopropylnaphthalene (2,6-DIPN) was observed over the immobilized aluminum chloride catalysts. Immobilization of aluminum chloride on mesoporous silica coupled with surface silylation is a promising way of developing alternative catalyst system for liquid-phase Friedel-Crafts alkylation reactions. (C) 2002 Elsevier Science B.V. All rights reserved.

Determination of domain sizes in blends of poly(ethylene) and poly(styrene) formed in the presence of supercritical carbon dioxide

Well-mixed blends of poly(ethylene) and poly(styrene) have been synthesized using supercritical carbon dioxide as a solvent. The morphology of the blends has been conclusively characterized using differential scanning calorimetry (DSC), small-angle X-ray scattering (SAXS), Raman microprobe microscopy, and C-13 solid-state cross-polarization magic angle spinning NMR (C-13 CPMAS NMR). DSC measurements demonstrate that poly(styrene) in the blends resides solely in the amorphous regions of the poly(ethylene) matrix; however, corroborative evidence from the SAXS experiments shows that poly(styrene) resides within the interlamellar spaces. The existence of nanometer-sized domains of poly(styrene) was shown within a blend of poly(styrene) and poly(ethylene) when formed in supercritical carbon dioxide using Raman microprobe microscopy and C-13 CPMAS NMR spectroscopy coupled with a spin diffusion model. This contrasts with blends formed at ambient pressure in the absence of solvent, in which domains of poly(styrene) in the micrometer size range are formed. This apparent improved miscibility of the two components was attributed to better penetration of the monomer prior to polymerization and increased swelling of the polymer substrate by the supercritical carbon dioxide solvent.

Manipulation and growth of birefringent protein crystals in optical tweezers

We report on the trapping, rotation, and in-situ growth of birefringent tetragonal lysozyme crystals in optical tweezers operating at a wavelength of 1070 nm. Variation of the pH and lysozyme concentration of the solution during growth was used to alter the length to width ratio of the crystals, and hence their orientation in the tweezers. Crystals with the optical axis skewed or perpendicular to the trapping-beam axis could be rotated by changing the orientation of linearly polarized light. We observed spontaneous spinning of some asymmetric crystals in the presence of linearly polarized light, due to radiation pressure effects. Addition of protein to the solution in the tweezers permitted real-time observation of crystal growth. (C) 2004 Optical Society of America.