Penciclovir solubility in Eudragit films: a comparison of X-ray, thermal, microscopic and release rate techniques

The solubility of penciclovir (C10N5O3H17) in a novel film formulation designed for the treatment of cold sores was determined using X-ray, thermal, microscopic and release rate techniques. Solubilities of 0.15–0.23, 0.44, 0.53 and 0.42% (w/w) resulted for each procedure. Linear calibration lines were achieved for experimentally and theoretically determined differential scanning calorimetry (DSC) and X-ray powder diffractometry (XRPD) data. Intra- and inter-batch data precision values were determined; intra values were more precise. Microscopy was additionally useful for examining crystal shape, size distribution and homogeneity of drug distribution within the film. Whereas DSC also determined melting point, XRPD identified polymorphs and release data provided relevant kinetics.

Using paleo-climate comparisons to constrain future projections in CMIP5

We present a description of the theoretical framework and "best practice" for using the paleo-climate model component of the Coupled Model Intercomparison Project (Phase 5) (CMIP5) to constrain future projections of climate using the same models. The constraints arise from measures of skill in hindcasting paleo-climate changes from the present over 3 periods: the Last Glacial Maximum (LGM) (21 thousand years before present, ka), the mid-Holocene (MH) (6 ka) and the Last Millennium (LM) (850–1850 CE). The skill measures may be used to validate robust patterns of climate change across scenarios or to distinguish between models that have differing outcomes in future scenarios. We find that the multi-model ensemble of paleo-simulations is adequate for addressing at least some of these issues. For example, selected benchmarks for the LGM and MH are correlated to the rank of future projections of precipitation/temperature or sea ice extent to indicate that models that produce the best agreement with paleoclimate information give demonstrably different future results than the rest of the models. We also find that some comparisons, for instance associated with model variability, are strongly dependent on uncertain forcing timeseries, or show time dependent behaviour, making direct inferences for the future problematic. Overall, we demonstrate that there is a strong potential for the paleo-climate simulations to help inform the future projections and urge all the modeling groups to complete this subset of the CMIP5 runs.

Assessing the completeness of optical resetting of quartz OSL in the natural environment

Resetting of previously accumulated optically stimulated luminescence (OSL) signals during transport of sediment is a fundamental requirement for reliable optical dating. The completeness of optical resetting of 46 modern-age quartz samples from a variety of depositional environments was examined. All equivalent dose (De) estimates were View the MathML source, with the majority of aeolian samples View the MathML source, and fluvial samples View the MathML source. The OSL signal of quartz originates from several trap types with different rates of charge loss during illumination. As such, incomplete bleaching may be identifiable as an increase in De from easy-to-bleach through to hard-to-bleach components. For all modern fluvial samples with non-zero De values, SAR De(t) analysis and component-resolved linearly modulated OSL (LM OSL) De estimates showed this to be the case, implying incomplete resetting of previously accumulated charge. LM OSL measurements were also made to investigate the extent of bleaching of the slow components in the natural environment. In aeolian sediments examined, the natural LM OSL was effectively zero (i.e. all components were fully reset). The slow components of modern fluvial samples displayed measurable residual signals up to 15 Gy.

Further investigations of the quartz optically stimulated luminescence components using linear modulation

The optically stimulated luminescence (OSL) from quartz is known to be the sum of several components with different rates of charge loss, originating from different trap types. The OSL components are clearly distinguished using the linear modulation (LM OSL) technique. A variety of pre-treatment and measurement conditions have been used on sedimentary samples in conjunction with linearly modulated optical stimulation to study in detail the behaviour of the OSL components of quartz. Single aliquots of different quartz samples have been found to contain typically five or six common LM OSL components when stimulated at View the MathML source. The components have been parameterised in terms of thermal stability (i.e. E and s), photoionisation cross-section energy dependence and dose response. The results of studies concerning applications of component-resolved LM OSL measurements on quartz are also presented. These include the detection of partial bleaching in young samples, use of ‘stepped wavelength’ stimulation to observe OSL from single components and attempts to extend the age range of quartz OSL dating.

An ASIFT-based local registration method for satellite imagery

Imagery registration is a fundamental step, which greatly affects later processes in image mosaic, multi-spectral image fusion, digital surface modelling, etc., where the final solution needs blending of pixel information from more than one images. It is highly desired to find a way to identify registration regions among input stereo image pairs with high accuracy, particularly in remote sensing applications in which ground control points (GCPs) are not always available, such as in selecting a landing zone on an outer space planet. In this paper, a framework for localization in image registration is developed. It strengthened the local registration accuracy from two aspects: less reprojection error and better feature point distribution. Affine scale-invariant feature transform (ASIFT) was used for acquiring feature points and correspondences on the input images. Then, a homography matrix was estimated as the transformation model by an improved random sample consensus (IM-RANSAC) algorithm. In order to identify a registration region with a better spatial distribution of feature points, the Euclidean distance between the feature points is applied (named the S criterion). Finally, the parameters of the homography matrix were optimized by the Levenberg–Marquardt (LM) algorithm with selective feature points from the chosen registration region. In the experiment section, the Chang’E-2 satellite remote sensing imagery was used for evaluating the performance of the proposed method. The experiment result demonstrates that the proposed method can automatically locate a specific region with high registration accuracy between input images by achieving lower root mean square error (RMSE) and better distribution of feature points.

Using palaeo-climate comparisons to constrain future projections in CMIP5

We present a selection of methodologies for using the palaeo-climate model component of the Coupled Model Intercomparison Project (Phase 5) (CMIP5) to attempt to constrain future climate projections using the same models. The constraints arise from measures of skill in hindcasting palaeo-climate changes from the present over three periods: the Last Glacial Maximum (LGM) (21 000 yr before present, ka), the mid-Holocene (MH) (6 ka) and the Last Millennium (LM) (850–1850 CE). The skill measures may be used to validate robust patterns of climate change across scenarios or to distinguish between models that have differing outcomes in future scenarios. We find that the multi-model ensemble of palaeo-simulations is adequate for addressing at least some of these issues. For example, selected benchmarks for the LGM and MH are correlated to the rank of future projections of precipitation/temperature or sea ice extent to indicate that models that produce the best agreement with palaeo-climate information give demonstrably different future results than the rest of the models. We also explore cases where comparisons are strongly dependent on uncertain forcing time series or show important non-stationarity, making direct inferences for the future problematic. Overall, we demonstrate that there is a strong potential for the palaeo-climate simulations to help inform the future projections and urge all the modelling groups to complete this subset of the CMIP5 runs.

Assessment of DGAT1 and LEP gene polymorphisms in three Nelore (Bos indicus) lines selected for growth and their relationship with growth and carcass traits

The aim of this study was to analyze LEP and DGAT1 gene polymorphisms in 3 Nelore lines selected for growth and to evaluate their effects on growth and carcass traits. Traits analyzed were birth, weaning, and yearling weight, rump height, LM area, backfat thickness, and rump fat thickness obtained by ultrasound. Two SNP in the LEP gene [LEP 1620(A/G) and LEP 305(T/C)] and the K232A mutation in the DGAT1 gene were analyzed. The sample consisted of 357 Nelore heifers from 2 lines selected for yearling weight and a control line, established in 1980, at the Estacao Experimental de Zootecnia de Sertaozinho (Sertaozinho, Brazil). Three genotypes were obtained for each marker. Differences in allele frequencies among the 3 lines were only observed for the DGAT1 K232A polymorphism, with the frequency of the A allele being greater in the control line than in the selected lines. The DGAT1 K232A mutation was associated only with rump height, whereas LEP 1620(A/G) was associated with weaning weight and LEP 305(T/C) with birth weight and backfat thickness. However, more studies, with larger data sets, are necessary before these makers can be used for marker-assisted selection.

Complex meiotic configuration of the holocentric chromosomes: the intriguing case of the scorpion Tityus bahiensis

Mitotic and meiotic chromosomes of Tityus bahiensis were investigated using light (LM) and transmission electron microscopy (TEM) to determine the chromosomal characteristics and disclose the mechanisms responsible for intraspecific variability in chromosome number and for the presence of complex chromosome association during meiosis. This species is endemic to Brazilian fauna and belongs to the family Buthidae, which is considered phylogenetically basal within the order Scorpiones. In the sample examined, four sympatric and distinct diploid numbers were observed: 2n = 5, 2n = 6, 2n = 9, and 2 = 10. The origin of this remarkable chromosome variability was attributed to chromosome fissions and/or fusions, considering that the decrease in chromosome number was concomitant with the increase in chromosome size and vice versa. The LM and TEM analyses showed the presence of chromosomes without localised centromere, the lack of chiasmata and recombination nodules in male meiosis, and two nucleolar organiser regions carrier chromosomes. Furthermore, male prophase I cells revealed multivalent chromosome associations and/or unsynapsed or distinctly associated chromosome regions (gaps, less-condensed chromatin, or loop-like structure) that were continuous with synapsed chromosome segments. All these data permitted us to suggest that the chromosomal rearrangements of T. bahiensis occurred in a heterozygous state. A combination of various factors, such as correct disjunction and balanced segregation of the chromosomes involved in complex meiotic pairing, system of achiasmate meiosis, holocentric nature of the chromosomes, population structure, and species dispersion patterns, could have contributed to the high level of chromosome rearrangements present in T. bahiensis.

Cloning and characterization of a zebrafish homologue of human AQP1: a bifunctional water and gas channel

Chen LM, Zhao J, Musa-Aziz R, Pelletier MF, Drummond IA, Boron WF. Cloning and characterization of a zebrafish homologue of human AQP1: a bifunctional water and gas channel. Am J Physiol Regul Integr Comp Physiol 299: R1163-R1174, 2010. First published August 25, 2010; doi:10.1152/ajpregu.00319.2010.-The mammalian aquaporins AQP1, AQP4, and AQP5 have been shown to function not only as water channels but also as gas channels. Zebrafish have two genes encoding an AQP1 homologue, aqp1a and aqp1b. In the present study, we cloned the cDNA that encodes the zebrafish protein Aqp1a from the 72-h postfertilization (hpf) embryo of Danio rerio, as well as from the swim bladder of the adult. The deduced amino-acid sequence of aqp1a consists of 260 amino acids and is 59% identical to human AQP1. By analyzing the genomic DNA sequence, we identified four exons in the aqp1a gene. By in situ hybridization, aqp1a is expressed transiently in the developing vasculature and in erythrocytes from 16 to 48 h of development. Later, at 72 hpf, aqp1a is expressed in dermal ionocytes and in the swim bladder. Western blot analysis of adult tissues reveals that Aqp1a is most highly expressed in the eye and swim bladder. Xenopus oocytes expressing aqp1a have a channel-dependent (*) osmotic water permeability (P(f)*) that is indistinguishable from that of human AQP1. On the basis of the magnitude of the transient change in surface pH (Delta pHS) that were recorded as the oocytes were exposed to either CO(2) or NH(3), we conclude that zebrafish Aqp1a is permeable to both CO(2) and NH(3). The ratio (Delta pHS*)CO2/P(f)* is about half that of human AQP1, and the ratio (Delta pHS*)NH3/P(f)* is about one-quarter that of human AQP1. Thus, compared with human AQP1, zebrafish Aqp1a has about twice the selectivity for CO(2) over NH(3).

Testing common nonlinear features in vector nonlinear autoregressive models

This paper studies a special class of vector smooth-transition autoregressive (VSTAR) models that contains common nonlinear features (CNFs), for which we proposed a triangular representation and developed a procedure of testing CNFs in a VSTAR model. We first test a unit root against a stable STAR process for each individual time series and then examine whether CNFs exist in the system by Lagrange Multiplier (LM) test if unit root is rejected in the first step. The LM test has standard Chi-squared asymptotic distribution. The critical values of our unit root tests and small-sample properties of the F form of our LM test are studied by Monte Carlo simulations. We illustrate how to test and model CNFs using the monthly growth of consumption and income data of United States (1985:1 to 2011:11).

A study on time-varying quantile and its applications

This Thesis is the result of my Master Degree studies at the Graduate School of Economics, Getúlio Vargas Foundation, from January 2004 to August 2006. am indebted to my Thesis Advisor, Professor Luiz Renato Lima, who introduced me to the Econometrics' world. In this Thesis, we study time-varying quantile process and we develop two applications, which are presented here as Part and Part II. Each of these parts was transformed in paper. Both papers were submitted. Part shows that asymmetric persistence induces ARCH effects, but the LMARCH test has power against it. On the other hand, the test for asymmetric dynamics proposed by Koenker and Xiao (2004) has correct size under the presence of ARCH errors. These results suggest that the LM-ARCH and the Koenker-Xiao tests may be used in applied research as complementary tools. In the Part II, we compare four different Value-at-Risk (VaR) methodologies through Monte Cario experiments. Our results indicate that the method based on quantile regression with ARCH effect dominates other methods that require distributional assumption. In particular, we show that the non-robust method ologies have higher probability to predict VaRs with too many violations. We illustrate our findings with an empirical exercise in which we estimate VaR for returns of São Paulo stock exchange index, IBOVESPA, during periods of market turmoil. Our results indicate that the robust method based on quantile regression presents the least number of violations.

Macroeconomia com M4

O trabalho trata de refazer modelos macroeconômicos usuais, como por exemplo o IS-LM, com a introdução de um agregado amplo de liqüidez, o M4. Faz-se uma cuidadosa análise da identidade de Walras, estendendo-se Simonsen (1983). Como resultado, vê-se que a introdução do mercado de M4 nos modelos exige que seja abandonada ou a curva IS (equilíbrio no mercado de bens e serviços),ou a LM (equilíbrio no mercado monetário). A seguir estuda-se a demanda de M4, sua estimativa no Brasil e um modelo LM4-LM. A utilidade da análise precedente é óbvia: podem-se estudar os impactos de aumentos de M4 diretamente, sem que se recorra ao efeito riqueza (Pigou).

Fatores relevantes na geração de valor da marca PETROBRAS - um estudo com consumidores do segmento de combustíveis

The main purpose of this study is identify and select the assessment indicators for Brand Equity (BE), in the fuels segment. Within this perspective, we have chosen to assess the influences based on the following attributes: Brand Loyalty (Lealdade à Marca ( LM), Brand Recognition (Conhecimento da Marca ( CM), Perceived Quality (Qualidade Percebida ( QP) and Brand Associations (Associações à Marca ( AM), through perception of consumers in Rio de Janeiro and São Paulo States. Based on deep interviews in the qualitative phase and in surveys, in the quantitative phase, we began the data analysis through Multiple Linear Regression, according to Atilgan et al. (2005) model and Ha (1996). In the first model, the (CM) was considered non-explanatory to (BE¿, and we¿ve chosen to remove it from the following study model. In the final model, besides remaining variables, demographic variables were included, such as: Family Income (Renda Familiar ( RF) and Federation State (Estado da Federação ( EF), as independent variables. The main relevant findings, through perception of consumers, indicated that the (BE) of company analyzed was better ranked in Rio de Janeiro than in São Paulo and, as for the income, higher level classes tend to have less understanding on the value of (BE) of Petrobras BR. The study finish with mentions the contribution of the research and management implication, as well as limitations and suggestions to further researchers.

A interação cultural : estudo de caso em uma empresa coreana no distrito industrial de Manaus

Trata-se de um estudo sobre a cultura organizacional em uma empresa coreana, a Samsung Eletrônica Amazônia S.A,. afiliada da Samsung Corporation, implantada no Distrito Industrial de Manaus, Estado do Amazonas, a partir de 1986, atraída pelos incentivos da Zona Franca de Manaus. A partir de uma pesquisa extensa, realizada em junho de 2001, pelo Setor de Recursos Humanos da empresa, foram selecionadas vinte e seis matrizes cujos resultados comprov_ lm a principal hipótese do trabalho: a de que a cultura da empresa pode ser a responsável pelos problemas existentes em sua área de produção, interferindo na qualidade de seus produtos. Das conclusões da pesquisa, portanto, infere-se que os dirigentes da empresa, todos coreanos, entram em choque com a cultura dos empregados na linha de produção, todos de naturalidade amazonense. Assim, os problemas entre essas duas culturas interferem negativamente em todos os indicadores da pesquisa, a saber: identidade, comunicação, liderança, percepção e interação.

Econometria espacial: discutindo medidas para a Matriz de ponderação espacial

Com o crescente uso das teorias e métodos vindos das áreas de economia regional e urbana, economia e econometria espacial na fronteira entre as áreas de economia e políticas públicas, estudar esse arcabouço teórico e metodológico torna-se relevante no campo da análise de políticas públicas. O objetivo geral dessa dissertação é discutir de modo crítico aspectos da literatura de econometria espacial e sua aplicabilidade, com especial foco na comparação entre os diferentes modelos e matrizes de ponderação espacial. Esse estudo procura analisar diferentes formas de medidas que permitem a construção de uma matriz de ponderação espacial, bem como testar por meio de simulação experimental e em uma aplicação empírica a robustez de algumas dessas matrizes e de diferentes modelos. Dadas as indicações da literatura e as evidências mostradas nessa dissertação, a estratégia mais confiável para a busca de um modelo parece ser utilizar as estimativas dos modelos, em conjunto com as estatísticas de Moran, para identificar presença de algum tipo de correlação espacial, e do teste LM da variável dependente, para indicar esse tipo de correlação especificamente. Observa-se que, de modo geral, não há muita diferença em termos de esperança dos parâmetros não espaciais, quando comparadas a estimações tradicionais com as que utilizam as técnicas espaciais. Com isso, a indicação que se pode fazer é que o uso das ferramentas de econometria espacial deve ser aplicado quando o interesse da análise é justamente na estimação dos parâmetros de correlação espacial. Comparando o uso da matriz de contigüidade no lugar da matriz de distâncias, é possível notar que a matriz de contigüidade não é, de forma geral, uma boa substituição para a matriz de distâncias. Ela capta parcialmente o efeito de rho e lambda, além de captar erroneamente lambda, quando este inexiste.