Cryptococcus: isolamento ambiental e caracterização bioquímica

Cryptococcosis is an opportunistic fungal infection caused by Cryptococcus yeasts, especially C. neoformans and Cryptococcus gattii. The fungus is found in substrates of animal and vegetable origin, and infection occurs through inhalation and seedlings present in the environment. The present study aimed to investigate the existence of microfocus Cryptococcus sp. from the environmental samples of Araçatuba city, São Paulo, featuring new niches, by decoupling the direct relationship between fungus and host in order to minimize the risk of contamination of man and animals, understanding the ecoepidemiology of Cryptococcus. Fifty samples from hollows and tree trunks were harvested (Cassia sp., Ficus sp., Caesalpinea peltophorides) from ten representatives in the urban perimeter. The samples were immediately sent to the Laboratory of Bacteriology and Mycology, Faculty of Veterinary Medicine Araçatuba - Unesp where they were processed and plated on Petri dishes containing agar seed Niger and Sabouraud dextrose agar with chloramphenicol, incubated at 30ºC for a period of no less than 5 days. Afterwards they were subimitted to biochemical tests: urease production, thermotolerance at 37°C and quimiotipagem in CGB agar (L- Canavanine-Glycine-Bromothymol blue). The results showed that 17 (34%) cultures were positive for Cryptococcus, 9 (18%) for Cryptococcus gattii and 8 (16%) for Cryptococcus neoformans. Other yeast correlated as Rhodotorula sp. and Candida sp. were isolated. We conclude that the infectious propagules of Cryptococcus are dispersed in nature and constitute an environmental microfocus, not necessarily being bound to a single host.

Unraveling Trichoderma species in attine ant environment: description of three new taxa

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Isolamento de leveduras em indústria de refrigerante e avaliação da susceptibilidade à ação antimicrobiana dos agentes sanificantes de uso industrial

Pós-graduação em Microbiologia - IBILCE

Transições reversíveis de células de leveduras entre estados viáveis, mas não cultiváveis (VBNC) e estados viáveis e cultiváveis

Pós-graduação em Biotecnologia - IQ

Diagnóstico de situação da produção de linguiça frescal suína no município de Rio Verde/GO

Pós-graduação em Medicina Veterinária - FCAV

Pré-tratamento e hidrólise da casca de uva para liberação de açúcares fermentescíveis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Atributos químicos, bioquímicos e microbiológicos em solos com 18 anos de aplicações anuais de lodo de esgoto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Leveduras patogênicas em fezes de aves de cativeiro: relação fenotípica e genotípica entre os isolados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Quality and safety of pet treats: assessment of the microbial safety and quality of pet treats

The aim of this study was to evaluate the hygienic and sanitary conditions of pettreats from an export industry. The occurrence of Salmonella, enumerated sulfite-reducing Clostridium (SRC), coagulase-positive Staphylococcus (CPS) and total(TC) and thermotolerant coliforms (TTC) was studied. Of the 108 samples ana-lyzed, 22 were contaminated by some microorganism. TC were detected at twosamples (3.5 × 101and 4.5 × 101colony-forming units [cfu]/g), SRC in 19 samples(1.0 × 101to 1.3 × 105cfu/g) and Salmonella spp. at only one sample; CPS andTTC were not detected. These findings indicate the microbiological safety of mostitems, except for a mix of dried bovine offal, with 1.3 × 105cfu/g of SRC, and onemix of dehydrated bovine viscera contaminated with Salmonella. The current pro-duction processes and the food safety management system are adequate to guar-antee the production of microbiologically safe foods, but that some improvementscan still be made with regard to cleaning and disinfection and hygiene training.

Método simples para quantificar o metabolismo aeróbio e aneróbio de levedura alcoólica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito do Ácido Ascórbico em melões 'Orange Flesh' minimamente processados

The aim of this work was to evaluate the application of different concentrations of ascorbic acid on Orange Flesh melon. Whole Melons were sanifi ed with 500 mg L-1 of sodium hypochlorite for 10 minutes and the cuts into cubes with 100 mg L-1 for 1 minute before being tested under different concentrations of ascorbic acid (0, 1, 2 and 3%) in immersion at room temperature for 10 minutes. After drainage, the cuts were packed in PET packages lined with polyethylene fi lm of 18 µm and stored at 5 ± 1°C and 85 ± 5% of RH for 8 days, being evaluated every 2 days. Physicochemical, microbiological and sensorial analyses were performed. The experimental design utilized for the experiment was the completely randomized in factorial scheme. Ten replicates were used for non-destructive analyses and 3 replicates were used for destructive ones. The application of ascorbic acid reduced the loss of mass; the fruits presented a low population of psychrotrophic bacterias, fi lamentous, fungi and yeasts, reduction of soluble solids, pH and fi rmness and, consequently, extended postharvest life of the fruits by 2 days. The appearance, fl avor and taste were also affected. The application of 1% of ascorbic acid was the best treatment for the fresh cuts “Orange Flesh” melons.

Quantificação do metabolismo aeróbio e anaeróbio de levedura alcoólica por método estequiométrico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tratamento de caldo e tipos de fermentos sobre os componentes secundários e qualidade da cachaça de alambique

Pós-graduação em Microbiologia Agropecuária - FCAV

Efeito da infecção com Candida albicans no desenvolvimento da Encefalomielite Autoimune Experimental

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de biofilme, perfil de sensibilidade e aspectos epidemiológicos de espécies fúngicas e algas do gênero Prototheca isoladas de casos de mastite bovina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)