Refuge Update – January/February 2006, Volume 3, Number 1

Table of Contents: Three New Species in AlaskaConserving Wildlife State by StateFocus on…Incident CommandMushing to Your New Job

Refuge Update – September/October 2008, Volume 5, Number 5

Table of Contents: Rebuilding after Hurricane Ike, page 3 Texas and Louisiana refuges were severely damaged in mid-September. A Crane Species Rebounding, page 5 At a Mississippi refuge, the world’s longest-running crane reintroduction program is succeeding. Focus on. . . Refuge System Wetlands, pages 8-13 The Refuge System manages wetlands to enhance their value for migratory waterfowl and shorebirds, threatened and endangered species and a myriad of native fish, wildlife, and plants. Fluttering Close to Extinction, page 17. Antioch Dunes Refuge is out to save the Lange’s metalmark butterfly.

Refuge Update – November/December 2005, Volume 2, Number 6

Table of Contents: Make Way for Ducklings, page 4 With help from refuge experts, roads and bridges can be built to accommodate wildlife. Katrina Heroes, pages 8-9 Extraordinary diaries from refuge staffers who were there when Katrina came calling. Focus on…Reaching Youth , page 10-15 Refuges give young people a chance to learn art, poetry, native culture, service – and stewardship. Nisqually: Growing and Restoring, page 17 The Outstanding Refuge Plan of 2005 opens the door to the largest estuary restoration project in the Pacific Northwest.

A diatom record of late Holocene climate variation in the northern range of Yellowstone National Park, USA

The changes in diatom species composition in a sediment core from Crevice Lake, Yellowstone National Park, spanning the past 2550 yr, were used to reconstruct long-term limnological and ecological conditions that may be related to late Holocene climate variability. Planktic forms dominate the fossil diatom assemblages throughout this record, but changes in species dominance indicate varying nutrient levels over time, particularly phosphorus. The changes in the nutrient concentrations in the lake were probably driven by changes in temperature and wind strength that affected the duration of watercolumn mixing and thus the extent of nutrient recycling from deep waters. Prior to 2100 cal before present (BP), Stephanodiscus minutulus and Synedra tenera dominated, suggesting long cool springs with extensive regeneration of phosphorus from the hypolimnion that resulted from isothermal mixing. From 2100 to 800 cal BP, these species were replaced by Cyclotella michiganiana and Cyclotella bodanica. These species are characteristic of lower nutrient concentrations and are interpreted here to reflect warm summers with long periods of thermal stratification. From 800 to 50 cal BP, S. minutulus dominated the diatom assemblage, suggesting a return to lengthy mixing during spring. The most dramatic late Holocene changes in the fossil diatom assemblages occurred during the transition from the Medieval Period to the Little Ice Age, approximately 800 cal BP.

URBAN WILDLIFE: CAN WE LIVE WITH THEM?

ABSTRACT: A survey of Extension Wildlife Specialists in the U.S. provided a basis for estimating the magnitude of urban wildlife damage and control in this country. Response to the 9-question mail questionnaire was good (76 percent) following the single mailing to all Extension Wildlife Specialists or people in similar positions listed in the national directory. The majority of questions were answered based upon the experiences and best estimates of these specialists for the interval October 1986-September 1987. Specialists had difficulty providing estimates of damage and costs of prevention and control; 57 percent were not able to provide any data on these topics. Several of the questions dealt with attitudes of people requesting urban wildlife information and/or assistance and wide ranges of responses were received to most of these questions. Most people (78 percent) appeared willing to implement prevention/control measures recommended by these specialists, more than half (61 percent) wanted the animal handled/removed by someone else, and only about 40 percent wanted the damage stopped regardless of cost. Also, slightly over half (55 percent) of clientele represented did not want the offending animal harmed in any way. These results were highly variable from state to state. Several differences were noted in overall responses regarding urban wildlife species. Requests for information were received most frequently for bats and snakes, but both of these groups of animals ranked very low in terms of actual damage reported. The most frequently mentioned groups of animals causing damage in urban areas were roosting birds (including pigeons, starlings, and sparrows), woodpeckers (especially flickers), tree squirrels, bats, and moles. In terms of actual dollar values of damage done, white-tailed deer and pocket gophers apparently caused the most estimated damage. Due to these differences, it is necessary to know which criteria are being used to make an assessment of the relative importance of animal damage control problems. Techniques for controlling urban wildlife damage, such as exclusion, live-trapping, repellents, and poisons, are compared and discussed in some detail in this paper. As urbanization occurs across the nation, concerns about urban wildlife damage will continue; in most cases, we can and will live among these creatures.

The Probe, Issue # 155 -- June 1995

Understanding the Eastern Coyote - Part II, by Thomas N. Tomsa, Jr., USDA-APHIS-ADC, Pennsylvania Book Review - "The Dirt Hole and Its Variations" All Texas Counties Quarantined for Rabies Ravenous Vultures Decimating Sheep & Calves on the East Coast Four-Year-Old Dies of Rabies The National Urban Wildlife Management Association (NUWMA) officially merged with NADCA, to create one larger, more effective organization to work for professional Animal Damage Control. State-Endangered Species: Meaningful Management or Preservationist Politics?, by Richard B. Chipman, Wildlife Biologist, USDA-APHIS-ADC, Vermont NADCA Membership Meeting Trapping Weasels <br>Jack H. Berryman 1995 Leopold Award Winner

The Probe, Issue 206 – November 1999

Wildlife Damage Conferences: When, Where, and Why? -- Robert M. Timm, Editor, THE PROBE Booklet Review:"The Problem with Skunks!!" by Edward Kellems (34 pages, illustrated. $14.95) New NWCO Web Page url is http://www.wildlifedamagecontrol.com/nwcoa.htm Abstracts from the 2nd International Wildlife Management Congress, Hungary Human Disturbance as a Design Factor to Aid Displacement of Canada Geese from Urban Parks -- P. C. Whitford, Biology Department, Capital University, Columbus, OH Leopard Problems in Nepal -- T. M. Maskey, National Parks and Wildlife Conservation Department, Kathmandu, Nepal Elk-human Conflict Management in Banff National Park, Alberta, Canada -- J. A. McKenzie, Banff National Park Wildlife Laboratory The Avoidance of Virtual Barriers by Wolves in Captivity -- M. Musiani*, E. Visalberghi*, andL. Boitani, *CNR Psychology Institute, Rome, Italy Successful Field Trials of a New Slow-Release Capsaicin-Based Animal Repellent for Reducing a Variety of Human-Wildlife Conflicts in Israel -- S. C. Nemtzov, Dept. of Terrestrial Ecology, The Nature and National Parks Protection Authority, Jerusalem, Israel Educational Workshops: A Proactive Approach to Conflict Resolution in Wildlife Management -- K. B. Reis, H. R. Campa III, R. B. Peyton, and S. Winterstein, Dept. of Fisheries & Wildlife, Michigan State University, East Lansing, MI Traps and Trapping in Sweden -- T. Svensson, Swedish Environmental Protection Agency, Stockholm, Sweden Actual Problems of Predator Management in Hungary -- L. Szemethy, M. Heltai, and Z. Biro, Dept. of Wildlife Biology & Management, Godollo University of Agricultural Sciences, Godollo, Hungary Crop and Livestock Depredation by Wildlife -- N. Udaya Sekhar, Centre for Int'I. Environment & Development Studies, Aas, Norway Conservation of the Iberian Wolf in Portugal—The Everlasting Conflict with Man -- J. V. Vingada*, C. Eira, S. Scheich, C. Fonseca, M. Soares, F. L. Correia, M. Fana* P. Carmo, A. Ferreira, A. Soares, and B. Bobek. *Dept. deBiologia da Universidade do Minho, Campus de Gualtar, Portugal Barkpeeling Damage in Relation to Red Deer Density and Forest Structure in Austria -- F. H. Voelk, Institute of Wildlife Biology & Game Management, Universitaetfuer Bodenkultur Wien, Vienna, Austria Human-Wildlife Conflict Resolution: National Imperatives and Strategies -- P. 0. Wander a Kenya Wildlife Service, Nairobi, Kenya An Overview and Evaluation of Deer Herd Management Programs in Urban and Suburban Communities of the USA -- R. J. Warren, Warnell School of Forest Resources, Univ. of Georgia, Athens, GA

The Probe, Issue 210 – May/June 2000

NADCA Election in Progress Berryman Institute Announces 1999 Awards New Probe Editor to be Appointed: Larry Sullivan Ken Garner Retires Book Review: "Master Land Snaring: Canine and Coon Techniques, Effective in All Terrains." by Newt Sterling as told to Bob Noonan. 1999. 58 pages, illustrated. NPCA Gets New Name Lobster Plates: PETA (People for the Ethical Treatment of Animals) is fighting to prevent the return of the lobster to Maine's automobile license plates. Active Antis in the Northwest Animal Rights Violence on Increase Abstracts from the 6th Annual Conference of The Wildlife Society: Monitoring of Sin Nombre Hantavirus in deer mice of the Southwest, USA -- Robinson, Rhonda /., Kathryn D. Bennett, James R. Biggs, Timothy K. Haarmann, David C. Keller, and Mary E. Salisbury Predators in the classroom: A prickly paradigm for educators -- Rollins, Dale Trends in bat rabies in the U.S.: Shaping public health policy -- Rupprecht, Charles E., Sharon B. Messenger, and Jean S. Smith

Development of site-specific sediment quality guidelines for North and South Atlantic littoral zones: Comparison against national and international sediment quality benchmarks

We aimed to develop site-specific sediment quality guidelines (SQGs) for two estuarine and port zones in Southeastern Brazil (Santos Estuarine System and Paranagua Estuarine System) and three in Southern Spain (Ria of Huelva, Bay of Cadiz, and Bay of Algeciras), and compare these values against national and traditionally used international benchmark values. Site-specific SQGs were derived based on sediment physical-chemical, toxicological, and benthic community data integrated through multivariate analysis. This technique allowed the identification of chemicals of concern and the establishment of effects range correlatively to individual concentrations of contaminants for each site of study. The results revealed that sediments from Santos channel, as well as inner portions of the SES, are considered highly polluted (exceeding SQGs-high) by metals, PAHs and PCBs. High pollution by PAHs and some metals was found in Sao Vicente channel. In PES, sediments from inner portions (proximities of the Ponta do Mix port`s terminal and the Port of Paranagua) are highly polluted by metals and PAHs, including one zone inside the limits of an environmental protection area. In Gulf of Cadiz, SQGs exceedences were found in Ria of Huelva (all analysed metals and PAHs), in the surroundings of the Port of CAdiz (Bay of CAdiz) (metals), and in Bay of Algeciras (Ni and PAHs). The site-specific SQGs derived in this study are more restricted than national SQGs applied in Brazil and Spain, as well as international guidelines. This finding confirms the importance of the development of site-specific SQGs to support the characterisation of sediments and dredged material. The use of the same methodology to derive SQGs in Brazilian and Spanish port zones confirmed the applicability of this technique with an international scope and provided a harmonised methodology for site-specific SQGs derivation. (C) 2009 Elsevier B.V. All rights reserved.

Tree height integrated into pantropical forest biomass estimates

Aboveground tropical tree biomass and carbon storage estimates commonly ignore tree height (H). We estimate the effect of incorporating H on tropics-wide forest biomass estimates in 327 plots across four continents using 42 656 H and diameter measurements and harvested trees from 20 sites to answer the following questions: 1. What is the best H-model form and geographic unit to include in biomass models to minimise site-level uncertainty in estimates of destructive biomass? 2. To what extent does including H estimates derived in (1) reduce uncertainty in biomass estimates across all 327 plots? 3. What effect does accounting for H have on plot- and continental-scale forest biomass estimates? The mean relative error in biomass estimates of destructively harvested trees when including H (mean 0.06), was half that when excluding H (mean 0.13). Power- and Weibull-H models provided the greatest reduction in uncertainty, with regional Weibull-H models preferred because they reduce uncertainty in smaller-diameter classes (< 40 cm D) that store about one-third of biomass per hectare in most forests. Propagating the relationships from destructively harvested tree biomass to each of the 327 plots from across the tropics shows that including H reduces errors from 41.8 Mg ha(-1) (range 6.6 to 112.4) to 8.0 Mg ha(-1) (-2.5 to 23.0).

Hyaluronidase splice variants are associated with histology and outcome in adenocarcinoma and squamous cell carcinoma of the lung

Heterogeneity of hyaluronidase (HYAL) expression has been identified in tumors and shows promise as an indicator of disease progression. The expression profile of alternatively spliced forms of HYAL was evaluated in tumors and normal lung tissue from 69 resected tumors of patients with adenocarcinomas and squamous cell carcinomas. HYAL1-wild-type (wt) and variants 1 to 5, HYAL2-wt, and HYAL3-wt, and variants 1 to 3 were identified by polymerase chain reaction and direct sequencing. Different proportions of the 3 HYAL-wt and variants were expressed in tumor and normal lung tissues. HYAL1-wt was associated with a poorer prognosis and HYAL3-vl with a better prognosis. HYAL splice variants are associated with histology and outcome, suggesting that strategies aimed at modulating their levels may be effective for lung cancer treatment. (C) 2012 Elsevier Inc. All rights reserved.

The Brazilian National Health Agency and the Mental Health Policy in the Context of the Private Health System: developments and challenges

This work analyses the mental health policy-making activity of the Brazilian National Health Agency (ANS), responsible for controlling health insurance companies. Three points are discussed: a) the framework of an economic and private health assistance regulatory activity, b) the ANS and its regulation activity and c) the rules produced by ANS in the mental health care field. It was concluded that, despite advances like the legal obligation to ensure medical treatment to all the diseases listed in ICD-10, the inclusion of suicidal patient damage and self-inflicted damage care, care provided by a multiprofessional team, the increase in the number of sessions with a psychologist, with an occupational therapist and of psychotherapy sessions, and mental health day hospitals included as part of the services offered, the authors identified specific regulatory gaps in this area. Some issues that ANS has to solve so that it can really play its institutional role of defending the public interest in the private health system are: the regulation of co-participation and franchise mechanisms, the increasing co-participation as a limitation of psychiatric hospitalization, and the limited number of crisis intervention psychotherapy sessions.

Echinococcus granulosus Antigen B Structure: Subunit Composition and Oligomeric States

Background: Antigen B (AgB) is the major protein secreted by the Echinococcus granulosus metacestode and is involved in key host-parasite interactions during infection. The full comprehension of AgB functions depends on the elucidation of several structural aspects that remain unknown, such as its subunit composition and oligomeric states. Methodology/Principal Findings: The subunit composition of E. granulosus AgB oligomers from individual bovine and human cysts was assessed by mass spectrometry associated with electrophoretic analysis. AgB8/1, AgB8/2, AgB8/3 and AgB8/4 subunits were identified in all samples analyzed, and an AgB8/2 variant (AgB8/2v8) was found in one bovine sample. The exponentially modified protein abundance index (emPAI) was used to estimate the relative abundance of the AgB subunits, revealing that AgB8/1 subunit was relatively overrepresented in all samples. The abundance of AgB8/3 subunit varied between bovine and human cysts. The oligomeric states formed by E. granulosus AgB and recombinant subunits available, rAgB8/1, rAgB8/2 and rAgB8/3, were characterized by native PAGE, light scattering and microscopy. Recombinant subunits showed markedly distinct oligomerization behaviors, forming oligomers with a maximum size relation of rAgB8/3 >rAgB8/2>rAgB8/1. Moreover, the oligomeric states formed by rAgB8/3 subunit were more similar to those observed for AgB purified from hydatid fluid. Pressure-induced dissociation experiments demonstrated that the molecular assemblies formed by the more aggregative subunits, rAgB8/2 and rAgB8/3, also display higher structural stability. Conclusions/Significance: For the first time, AgB subunit composition was analyzed in samples from single hydatid cysts, revealing qualitative and quantitative differences between samples. We showed that AgB oligomers are formed by different subunits, which have distinct abundances and oligomerization properties. Overall, our findings have significantly contributed to increase the current knowledge on AgB expression and structure, highlighting issues that may help to understand the parasite adaptive response during chronic infection.

Exotic B-c-like molecules in QCD sum rules

We use the QCD sum rules to study possible B-c-like molecular states. We consider isoscalar J(P) = 0(+) and J(P) = 1(+) D(*) B(*) molecular currents. We consider the contributions of condensates up to dimension eight and we work at leading order in alpha(s). We obtain for these states masses around 7 GeV. (C) 2012 Elsevier B.V. All rights reserved.

The Aurora A and B kinases are up-regulated in bone marrow-derived chronic lymphocytic leukemia cells and represent potential therapeutic targets

Background The malignant B cells in chronic lymphocytic leukemia receive signals from the bone marrow and lymph node microenvironments which regulate their survival and proliferation. Characterization of these signals and the pathways that propagate them to the interior of the cell is important for the identification of novel potential targets for therapeutic intervention. Design and Methods We compared the gene expression profiles of chronic lymphocytic leukemia B cells purified from bone marrow and peripheral blood to identify genes that are induced by the bone marrow microenvironment. Two of the differentially expressed genes were further studied in cell culture experiments and in an animal model to determine whether they could represent appropriate therapeutic targets in chronic lymphocytic leukemia. Results Functional classification analysis revealed that the majority of differentially expressed genes belong to gene ontology categories related to cell cycle and mitosis. Significantly up-regulated genes in bone marrow-derived tumor cells included important cell cycle regulators, such as Aurora A and B, survivin and CDK6. Down-regulation of Aurora A and B by RNA interference inhibited proliferation of chronic lymphocytic leukemia-derived cell lines and induced low levels of apoptosis. A similar effect was observed with the Aurora kinase inhibitor VX-680 in primary chronic lymphocytic leukemia cells that were induced to proliferate by CpG-oligonucleotides and interleukin-2. Moreover, VX-680 significantly blocked leukemia growth in a mouse model of chronic lymphocytic leukemia. Conclusions Aurora A and B are up-regulated in proliferating chronic lymphocytic leukemia cells and represent potential therapeutic targets in this disease.