Hyaluronidase splice variants are associated with histology and outcome in adenocarcinoma and squamous cell carcinoma of the lung

Heterogeneity of hyaluronidase (HYAL) expression has been identified in tumors and shows promise as an indicator of disease progression. The expression profile of alternatively spliced forms of HYAL was evaluated in tumors and normal lung tissue from 69 resected tumors of patients with adenocarcinomas and squamous cell carcinomas. HYAL1-wild-type (wt) and variants 1 to 5, HYAL2-wt, and HYAL3-wt, and variants 1 to 3 were identified by polymerase chain reaction and direct sequencing. Different proportions of the 3 HYAL-wt and variants were expressed in tumor and normal lung tissues. HYAL1-wt was associated with a poorer prognosis and HYAL3-vl with a better prognosis. HYAL splice variants are associated with histology and outcome, suggesting that strategies aimed at modulating their levels may be effective for lung cancer treatment. (C) 2012 Elsevier Inc. All rights reserved.

Brazilian agency National Council for Scientific and Technological Development [CNPq 300430/95-7]

Brazilian agency National Council for Scientific and Technological Development

Foundation for the Support of Research of the State of Sao Paulo [FAPESP 08/5791-7, 08/58557-2]

Foundation for Research Support of the State of Sao Paulo

Brazilian agency Laboratories for Medical Research(LIMs), Hospital das Clinicas, University of Sao Paulo Medical School

Brazilian agency Laboratories for Medical Research(LIMs), Hospital das Clinicas, University of Sao Paulo Medical School