Effects of GHR gene polymorphisms on growth and carcass traits in Zebu and crossbred beef cattle

The growth hormone receptor (GHR) is the cell surface receptor for growth hormone (GH) and is required for GH to carry out its effects on target tissues. The objectives of the present study were to estimate the allele and genotype frequencies of the GHR/Alu I gene polymorphism located in the regulatory region in beef cattle belonging to different genetic groups and to determine associations between this polymorphism and growth and carcass traits. Genotyping was performed on 384 animals, including 79 Nellore (Zebu), 30 Canchim (5/8 Charolais+3/8 Zebu), 30 Simmental X Nellore crossbred and 245 Angus x Nellore crossbred cattle. Alleles Alu I(+), Alu I(-) and Alu I(N)-null allele-were evidenced for the GHR/Alu I polymorphism and the frequency of the Alu I(N) allele was significantly higher than the frequency of the Alu I(+) and Alu I(-) alleles in all genetic groups. Genotype Alu I(N/N) of the GHRIAlu I predominated in Nellore animals, while the Alu I(N/+) and Alu I(N/-) predominated in the other genetic groups. In the association studies, traits of interest were analyzed using the General Linear Model (GLM) procedure of the SAS program and least squares means of the genotypes were compared by the Tukey test. Significant associations (P < 0.05) were observed between the Alu I(N/N) genotype of the GHRIAlu I polymorphism and lower weight gain and body weight at slaughter, although a confounding between genotypes and genetic groups may have occurred. (c) 2005 Elsevier B.V. All rights reserved.

Comparison among three approaches for evaluation of sexual precocity in Nellore cattle

Restricted breeding seasons used in beef cattle produce censored data for reproduction traits measured in regard to these seasons. To analyze these data, adequate methods must be used. The objective of this paper was to compare three approaches aiming to evaluate sexual precocity in Nellore cattle. The final data set contained 6699 records of age at first conception (AFC14) (in days) and of heifer pregnancy (HP14) (binary) obtained from females exposed to the bulls for the first time at about 14 months of age. Records of females that did not calve in the following year after being exposed to a sire were considered censored (77.5% of total). The models used to obtain genetic parameters and expected progeny differences (EPDs) were a Weibull mixed and a censored linear model for AFC14 and threshold model for HP14. The mean heritabilities obtained were 0.76 and 0.44, respectively, for survival and censored linear models (for AFC14), and 0.58 for HP14. Ranking and Pearson correlations varied (in absolute values) from 0.54 to 0.99 (considering different percentages of sires selected), indicating moderate changes in the classification. Considering survival analysis as the best selection criterion (that would result in the best response to selection), it was observed that selection for HP14 would lead to a more significant decrease in selection response if compared with selection for AFC14 analysed by censored linear model, from which results were very similar to the survival analysis.

Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Associations between LEP, DGAT1 and FABP4 gene polymorphisms and carcass and meat traits in Nelore and crossbred beef cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bovine gene polymorphisms related to fat deposition and meat tenderness

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Growth and carcass traits associated with GH1/Alu I and POU1F1/Hinf I gene polymorphisms in Zebu and crossbred beef cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência das cargas selecionadas na determinação da potência crítica determinada no ergômetro de braço em dois modelos lineares

O objetivo deste estudo foi verificar o efeito da seleção das cargas e do modelo utilizado para a determinação da PC no ergômetro de braço. Participaram do estudo oito voluntários do sexo masculino, que praticavam atividade física regularmente e eram aparentemente saudáveis. Os sujeitos realizaram quatro testes com cargas constantes mantidas até a exaustão voluntária no ergômetro de braço UBE 2462-Cybex. As cargas foram individualmente selecionadas para induzir a fadiga entre 1 e 15 minutos. Para cada sujeito, a determinação da PC foi realizada através de dois modelos lineares: potência-1/tempo e trabalho-tempo. em cada um dos modelos, foram utilizadas todas as potências (1), as três maiores (2) e as três menores (3). As PC encontradas no modelo potência-1/tempo e trabalho-tempo para a condição 3 (177,5 + 29,5; 173,9 + 33,3, respectivamente) foram significantemente menores do que as da condição 2 (190,5 + 23,2; 183,4 + 22,3, respectivamente), não existindo diferenças destas com as da condição 1 (184,2 + 25,4; 176,4 + 28,8, respectivamente). As PC determinadas no modelo potência-1/tempo para as condições 1 e 2 foram significantemente maiores do que as determinadas no modelo trabalho-tempo, não existindo diferenças para a condição 3. Pode-se concluir que as cargas selecionadas e o modelo utilizado interferem na determinação da PC encontrada no ergômetro de braço, podendo interferir no tempo de exaustão durante o exercício submáximo realizado em cargas relativas a este índice.

Financial comparisons of the artisanal fisheries in Urubupungá Complex in the middle Paraná River (Brazil)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação de modelo matemático para estimar a radiação solar incidente sobre superfícies com diferentes exposições e declividades

Este trabalho teve por objetivo avaliar modelo matemático para estimar a radiação solar global diária sobre superfícies com diferentes exposições e declividades, no período de março de 2002 a março de 2003. A pesquisa foi desenvolvida em uma estrutura denominada Bacia Hidrográfica Experimental do Departamento de Engenharia Rural da UNESP, Câmpus de Jaboticabal - SP. Nessa estrutura, foram utilizadas superfícies caracterizadas como H, 10N, 10S, 20N, 20S, 10E, 10W, 20E e 20W. O sensor utilizado para medir a radiação solar global incidente nas superfícies estudadas foi um piranômetro da marca Kipp & Zonnen, modelo CM3. Para calcular a radiação solar incidente nas superfícies estudadas, foi utilizado o modelo de Kondratyev. As análises dos resultados foram feitas para o período diário, utilizando-se de análise de regressão e considerando o modelo linear (y = ax + b), na qual a variável dependente foi a radiação global medida (K¯M) e a radiação global calculada (K¯C) foi a variável independente. Os resultados deste estudo mostram que o modelo apresentou bons resultados para estimar a radiação nas superfícies H, 10N, 10S, 10E, 10W, 20E e 20W. Utilizando-se de dados de dias com céu límpido, foram obtidos os seguintes resultados: no inverno, o modelo foi preciso para estimar a radiação solar na superfície 20N, e apresentou resultados aceitáveis para estimar a radiação solar na superfície 20S.

Late administration of a specific COX-2 inhibitor does not treat and/or prevent progression of gastric tumors in rats submitted to duodenogastric reflux procedure

PURPOSE:To assess whether late introduction of a specific COX-2 inhibitor (Meloxicam) can treat and/or prevent the progression of tumors in the stomach of rats submitted to duodenogastric reflux. METHODS: Seventy five male Wistar rats, weighing 150 grams, were submitted to the induction of duodenogastric reflux through the pylorus. At 36 weeks of follow-up were established three experimental groups: DGR36 sacrificed immediately, DGR54 and DGR54MLX both sacrificed at 54th week of follow-up . The animals of the latter group were fed with a rat chow premixed with Meloxicam (2.0 mg/ kg feed; 0.3 mg / kg bw / day) and the other two with standard rat chow. The lesions found in the pyloric mucosa and gastrojejunal anastomosis were analyzed macroscopically and histologically. For statistical analysis was adjusted a generalized linear model assuming a binomial distribution with LOGIT link function. RESULTS: No significant differences were found when comparing the incidences of benign tumor lesions (Adenomatous Hyperplasia), p=0.4915, or malignant (Mucinous Adenocarcinoma), p=0.2731, among groups. CONCLUSION: Late introduction of specific COX-2 inhibitor (Meloxicam) did not treat and was not able to prevent the progression of tumoral lesions induced by duodenogastric reflux in the rat stomachs.

ANÁLISE DE REGRESSÃO em EXPERIMENTOS em PARCELAS SUBDIVIDIDAS COM DADOS NÃO-BALANCEADOS: UMA SOLUÇÃO NA LINGUAGEM GLIM

São estabelecidas as matrizes necessárias para a realização da análise de variância de experimentos em parcelas subdivididas, com dados não-balanceados e balanceados, quando os tratamentos aplicados às parcelas e os tratamentos aplicados às subparcelas são ambos fatores quantitativos, usando a teoria de modelos lineares e de modelos lineares generalizados. Foi desenvolvido um programa computacional, na linguagem GLIM, para a realização da análise.

Methods to verify parameter equality in nonlinear regression models

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Structure of luminescent mono and di-urethanesil nanocomposites doped with Eu3+ ions

A structure modeling of two families of sol-gel derived Eu3+-doped organic/inorganic hybrids based on the results of small-angle X-ray scattering experiments is reported. The materials are composed of poly(oxyethylene) chains grafted at one or both ends to siloxane groups and are called mono- and di-urethanesils, respectively. A theoretical function corresponding to a two-level hierarchical structure model fits well the experimental Scattering curves. The first level corresponds to small siloxane clusters embedded in a polymeric matrix. The secondary level is associated to the existence of siloxane cluster rich domains surrounded by a cluster-depleted polymeric matrix. Results show that increasing europium doping favors the growth of the secondary domains. (C) 2002 Elsevier B.V. B.V. All rights reserved.

Effects of sex and locality on the abundance of lice on the wild rodent Oligoryzomys nigripes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A comunicação midiática da tecnociência: uma leitura a partir do enfoque cts

This paper has two basic aims. The first one is to propose a discussion about the journalistic potential of information regarding patents, trying to identify using some experiences, the characteristics of this techno-scientific product that, somehow, draws the attention of the media. The second one is to understand the relationship between media and patents from the perspective of the studies on Science, Technology and Society (STS). The following assumption is made: the media treatment of patents is still strongly marked by economic bias and follows the logic of the supply-linear model, under which, among other important ideas, basic research is followed by applied research and, then, by technological developments that allow the launch of a new product bringing benefits for society. The paper presents results from case studies of science communication involving patents of the Federal University of Silo Carlos (UFSCar), Brazil, indicating that they are an important source of news for the media, and emphasizing the importance of the media in the national and international discussions about the protection of intellectual work.