Distribution of exoantigens and a 43-kDa glycoprotein (gp43) in the yeast and mycelial forms of Paracoccidioides brasiliensis

Objective. Paracoccidioides brasiliensis antigens (strain 113) were located at ultrastructural level in both yeast and mycelial forms of the fungus. The reactivity of the sera employed was analysed. Materials and methods. Immunofluorescence and ultrastructural protein A-gold immunolabelling techniques were performed using two polyclonal antisera: one against P. brasiliensis exoantigens and the other against a 43-kDa glycoprotein (gp43). Immunoblotting assays were employed to define reactivity of these antisera with somatic and metabolic antigens of both forms of the fungus. Results. The techniques employed revealed in both yeast and mycelial forms of P. brasiliensis a similar antigenic distribution. The antigens deposits were seen within the cytoplasm, and over the cell wall of the fungus. The anti-exoantigen serum recognized several bands in both forms of the fungus. The anti-gp43 serum reacted strongly with the 43-kDa fraction and weakly with few other fractions. Conclusions. Immunocytochemical techniques suggest a protein synthesis within the cytoplasm followed by excretion through the cell wall. Similar results employing both polyclonal antisera were obtained.

Human chromosome aberrations induced in vitro by Paracoccidioides brasiliensis glycoproteic component (GP 43)

The in vitro cytogenetic effects of the 43-kDa molecular mass exocellular glycoproteic component (GP 43) from Paracoccidioides brasiliensis were studied in cultures from human lymphocytes. The sample included 10 healthy, white, non-smoking, non-related males (mean age of 31.3 ± 8.2 years). Besides the control, three concentrations of GP 43 (0.125, 1.25 and 5 μg/ml) were used. In each group, around 1000 cells were examined in search of chromosome aberrations, and 30,000 metaphases were analysed for the determination of the Mitotic Index. The authors conclude that GP 43 most probably causes inhibition of the cell cycle and aneugenic and clastogenic effects.

Notas de la CEPAL Nº 43

Disminuye el número de pobres en América Latina desde 2003 Con gran éxito cerró primer ciclo del concurso Experiencias en innovación social Columna de Opinión: Avances y desafíos de la sociedad de la información en América Latina y el Caribe, por José Luis Machinea Precisiones: El patrón de consumo energético es preocupantemente contaminante en la región, por Hugo Altomonte Indicadores La sociedad de la información: una oportunidad para crecer con equidad e inclusión social Publicaciones recientes Calendario

ECLAC Notes Nº 43

The Number of Poor People in Latin America Has Fallen by 13 Million Since 2003 First Round of "Experiences in Social Innovation" Competition Successfully Concludes Op-ed by ECLAC's Executive Secretary, José Luis Machinea. The Information Society in Latin America and the Caribbean: Progress and Challenges Highlights. Polluting Energy Consumption Pattern in Latin America and the Caribbean Cause for Concern Indicators The Information Society Offers the Chance to Grow with Equity and Social Inclusion Recent Titles Calendar of Events

Desempenho inicial da laranjeira Valência sobre 43 porta-enxertos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Carta Circular de la Red de Cooperación en la Gestión Integral de Recursos Hídricos para el Desarrollo Sustentable en América Latina y el Caribe N° 43

Editorial.-- Discusión abierta: Eficiencia energética y regulación económica en los servicios de agua potable ; Políticas e institucionalidad en materia de agua potable y saneamiento en la región.-- Noticias de la RED: Los países del Golfo Arábico y su objetivo de autosuficiencia alimentaria ; Hacia la reducción de las brechas en agua potable y saneamiento en Colombia ; Presupuesto participativo en agua potable: una oportunidad para los usuarios ; Ley de las organizaciones de usuarios de aguas del Perú ; Comisión de gestión integral de la Cuenca del Río Grande de Tárcoles en Costa Rica ; Unidad Gestora de agua y saneamiento en Panamá ; Cursos: Curso "Aspectos estratégicos del riego".-- Noticias sobre internet y WWW

Network for Cooperation in Integrated Water Resource Management for Sustainable Development in Latin America and the Caribbean No. 43

Editorial remarks.-- Open discussion: Energy efficiency and economic regulation in water supply and sewerage services ; Policies and institutional frameworks for drinking water supply and sanitation.-- News of the network: The goal of food self-sufficiency of the countries of the cooperation council for de Arab States of the Gulf ; Narrowing the gaps in drinking water and sanitation: an opportunity for users ; Water Users'Organizations Act of Peru ; Commission for the Integrated management of the Tárcoles River Basin, Costa Rica ; Water and sanitation Management Unit in Panama.-- Courses: Course "Strategic aspects of irrigation".-- Internet and WWW news

Estudo comparativo de isolados de P. brasiliensis em adesão celular. Sinalização celular mediada pela GP 43

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR