Human chromosome aberrations induced in vitro by Paracoccidioides brasiliensis glycoproteic component (GP 43)


Autoria(s): Villalba Freire-Maia, Dertia; Aparecida Spadotto Balarin, Marly; José Mendes-Giannini, Maria
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

24/04/1997

Resumo

The in vitro cytogenetic effects of the 43-kDa molecular mass exocellular glycoproteic component (GP 43) from Paracoccidioides brasiliensis were studied in cultures from human lymphocytes. The sample included 10 healthy, white, non-smoking, non-related males (mean age of 31.3 ± 8.2 years). Besides the control, three concentrations of GP 43 (0.125, 1.25 and 5 μg/ml) were used. In each group, around 1000 cells were examined in search of chromosome aberrations, and 30,000 metaphases were analysed for the determination of the Mitotic Index. The authors conclude that GP 43 most probably causes inhibition of the cell cycle and aneugenic and clastogenic effects.

Formato

5-10

Identificador

http://dx.doi.org/10.1016/S0165-1218(96)00148-6

Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 390, n. 1-2, p. 5-10, 1997.

1383-5718

http://hdl.handle.net/11449/65090

10.1016/S0165-1218(96)00148-6

WOS:A1997WV93700002

2-s2.0-0030973326

Idioma(s)

eng

Relação

Mutation Research: Genetic Toxicology and Environmental Mutagenesis

Direitos

closedAccess

Palavras-Chave #Chromosome aberration #Cytogenetics #GP 43 #Mitotic index #Mutagenesis #Paracoccidioides brasiliensis #Paracoccidioidomycosis #glycoprotein #adult #chromosome aberration #controlled study #cytogenetics #human #human cell #in vitro study #lymphocyte culture #male #mutagenesis #normal human #paracoccidioides brasiliensis #priority journal #structural chromosome aberration #Adult #Aneuploidy #Antigens, Fungal #Cells, Cultured #Chromosome Aberrations #Fungal Proteins #Glycoproteins #Humans #Lymphocytes #Male #Metaphase #Mitotic Index #Mutagenicity Tests #Oligosaccharides
Tipo

info:eu-repo/semantics/article