Phenotypic and metabolic aspects of prostatic epithelial cells in aged gerbils after antisteroidal therapy: Turnover in the state of chromatin condensation and androgen-independent cell replacement

The gerbil is a rodent considered a good model for studies of prostatic morphophysiology under different experimental conditions. Studies involving castration and steroidal blockers of aged gerbils showed that the glandular epithelium persists after long-term therapy, preventing the organ atrophy. Thus, the objective of this study was to evaluate the phenotypic characteristics and behavior of prostatic epithelial cells that remained after different periods of hormone ablation in aged gerbils. The identification of elements that influenced the survival of this cell type was performed by morphometric, nuclear phenotypes, ultrastructural and immune histochemical analysis. The most significant responses to treatment, by analyzing morphometric features, were observed during the first three time points (day 1, day 3, and day 7), after which there appeared to be an adjustment of the gland to the hormone ablation. All treatments led to changes in the state of chromatin condensation, DNA methylation pattern and phenotypic changes indicated cell senescence. Additionally, an increase in the basal cells seemed to guarantee self-renewal properties to the epithelium. These data indicate that changes occur at many levels, including gene expression and nuclear architecture in the epithelial cells, when aging and steroidal blockade are associated. These aspects are important when considering castration-resistant prostate cancer, a malignant tumor posing difficult therapeutic intervention. © 2013 Elsevier GmbH. All rights reserved.

A dramaturgia do trabalho no teatro paulistano contemporâneo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Informe del Taller de Trabajo La dimensión ambiental en la planificación del desarrollo del área de influencia del aprovechamiento múltiple de Salto Grande (Argentina-Uruguay) = Report of the Workshop on "The environmental dimension in the development planning of the area of influence of the multiple exploitation of Salto Grande (Argentina-Uruguay)""

Contiene las conclusiones y recomendaciones del Taller que tuvo por objetivo analizar las politicas de desarrollo y sus perspectivas en el area de influencia del aprovechamiento multiple de Salto Grande, con el fin de recomendar las vias mas favorables para incorporar la dimension ambiental en los procesos de planificacion.

Análise energética do processo experimental de produção de biodiesel a partir de óleo de frango

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

The status of biodiversity and bioprospecting efforts in the Caribbean subregion

Includes bibliography

Deficiência hídrica em plantas de pimentão (Capsicum annum L.) fertirrigadas e seus efeitos sobre a produção de massa e parâmetros bioquímicos

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Interface entre políticas públicas de saúde mental e economia solidária: a inserção socioprodutiva de pessoas com transtornos mentais

A interface trabalho e loucura têm sido construída e reconstruída ao longo da história, diante disto, o uso do trabalho não se constitui uma novidade no campo da saúde mental, ele está relacionado ao nascimento da psiquiatria, em um contexto de transformações das relações de produção, com a justificativa e finalidade de controle social, exploração de mão de obra e tratamento moral.No entanto, a partir do Processo de Reforma Psiquiátrica o trabalho entra em cena sob novas perspectivas. Nesse sentido, o presente estudo aborda a relação entre as políticas de saúde mental em que o trabalho é associado à economia solidária, a fim de compreender o sentido atribuído ao trabalho relacionado, buscando identificar suas práticas de inserção socioprodutiva e como é incorporado ao campo da saúde mental no Estado do Pará. Trata-se de uma pesquisa qualitativa, com base em entrevistas, observações in locos, levantamento de material documental e institucional. Paralelamente, efetivouse o tratamento do material recolhido, com o intuito de ordenar os dados obtidos, de evidenciar as experiências observadas por meios das oficinas de trabalho e/ou produção desenvolvidas nos serviços substitutivos, bem como, por meio das associações e cooperativas. Obteve-se como resultado as atividades sociais e produtivas relacionadas à saúde mental fazem parte dos serviços desenvolvidos nos CAPS, mas também são incentivadas a partir desses espaços como é o caso da Brilho e Luz, única associação de pessoas com transtornos mentais. Malgrado, se mostrarem incipientes e frágeis em sua constituição, necessitando de infraestrutura adequada e recursos, portanto, sem possibilidade de autonomia e independência em relação aos seus parceiros, principalmente governamentais, verifiquem-se aspectos positivos no âmbito individual das pessoas inseridas. Importa ressaltar que são experiências recentes no Brasil, sobretudo, no estado do Pará enquanto políticas públicas de saúde mental em interface com a economia solidária, constituindo-se em um processo em construção.

Enzyme replacement therapy for Mucopolysaccharidosis Type I among patients followed within the MPS Brazil Network

Mucopolysaccharidosis type I (MPS I) is a rare lysosomal disorder caused by deficiency of alpha-L-iduronidase. Few clinical trials have assessed the effect of enzyme replacement therapy (ERT) for this condition. We conducted an exploratory, open-label, non-randomized, multicenter cohort study of patients with MPS I. Data were collected from questionnaires completed by attending physicians at the time of diagnosis (T1; n = 34) and at a median time of 2.5 years later (T2; n = 24/34). The 24 patients for whom data were available at T2 were allocated into groups: A, no ERT (9 patients; median age at T1 = 36 months; 6 with severe phenotype); B, on ERT (15 patients; median age at T1 = 33 months; 4 with severe phenotype). For all variables in which there was no between-group difference at baseline, a delta of ≥ ± 20% was considered clinically relevant. The following clinically relevant differences were identified in group B in T2: lower rates of mortality and reported hospitalization for respiratory infection; lower frequency of hepatosplenomegaly; increased reported rates of obstructive sleep apnea syndrome and hearing loss; and stabilization of gibbus deformity. These changes could be due to the effect of ERT or of other therapies which have also been found more frequently in group B. Our findings suggest MPS I patients on ERT also receive a better overall care. ERT may have a positive effect on respiratory morbidity and overall mortality in patients with MPS I. Additional studies focusing on these outcomes and on other therapies should be performed.

Mucopolysaccharidoses in northern Brazil: targeted mutation screening and urinary glycosaminoglycan excretion in patients undergoing enzyme replacement therapy

Mucopolysaccharidoses (MPS) are rare lysosomal disorders caused by the deficiency of specific lysosomal enzymes responsible for glycosaminoglycan (GAG) degradation. Enzyme Replacement Therapy (ERT) has been shown to reduce accumulation and urinary excretion of GAG, and to improve some of the patients' clinical signs. We studied biochemical and molecular characteristics of nine MPS patients (two MPS I, four MPS II and three MPS VI) undergoing ERT in northern Brazil. The responsiveness of ERT was evaluated through urinary GAG excretion measurements. Patients were screened for eight common MPS mutations, using PCR, restriction enzyme tests and direct sequencing. Two MPS I patients had the previously reported mutation p.P533R. In the MPS II patients, mutation analysis identified the mutation p.R468W, and in the MPS VI patients, polymorphisms p.V358M and p.V376M were also found. After 48 weeks of ERT, biochemical analysis showed a significantly decreased total urinary GAG excretion in patients with MPS I (p < 0.01) and MPS VI (p < 0.01). Our findings demonstrate the effect of ERT on urinary GAG excretion and suggest the adoption of a screening strategy for genotyping MPS patients living far from the main reference centers.

Assessing the replacement of lead in solders: effects on resource use and human health

Human health and environmental concerns are not usually considered at the same time. Tin-lead solders are still widely used in several countries, including Brazil, by manufacturers of electronic assemblies. One of the options to reduce or eliminate lead from the manufacturing environment is its replacement with lead-free alloys. This paper applies emergy synthesis and the DALY indicator (Disability Adjusted Life Years) to assess the impact of manufacturing soft solder using tin, lead and other metals on the environment and on human health. The results are presented together with the company's financial results and the results calculated from the Brazilian statistical value of life. The calculation of emergy per unit showed that more resources are used to produce one ton of lead-free solders than to produce one ton of tin-lead solders, with and without the use of consumer waste recovered through a reverse logistics system. The assessment of air emissions during solder production shows that the benefits of the lead-free solution are limited to the stages of manufacturing and assembling. The tin-lead solder appears as the best option in terms of resource use efficiency and with respect to emissions into the atmosphere when the mining stage is included. A discussion on the influence of the system's boundaries on the decision-making process for materials substitution is presented. (C) 2012 Elsevier Ltd. All rights reserved.

Fishery of the Uçá Crab Ucides Cordatus (Linnaeus, 1763) in a Mangrove Area in Cananéia, State of São Paulo, Brazil: Fishery Performance, Exploitation Patterns and Factors Affecting the Catches

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of catecholamines on volemic replacement with saline solution and the impact on heart rate variability in rabbits subjected to hemorrhage. A study by spectral analysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mini-implants as provisional anchorage for the replacement of missing anterior teeth: A clinical report

This clinical report describes an adult patient referred for orthodontic treatment with mini-implants as anchorage to correct the root angulation of maxillary lateral incisors. The purpose of this report was to demonstrate the versatility of mini-implants placed in a vertical direction in esthetic areas. During orthodontic treatment, some aspects must be observed to preserve the interim restoration against the occlusal loads to avoid screw fracture. A fixed appliance was placed to correct the position of the maxillary anterior teeth and to complete the treatment. Acceptable esthetics and function were achieved.

Effects of neem oil (Azadirachta indica A. Juss) on the replacement of the midgut epithelium in the lacewing Ceraeochrysa claveri during larval-pupal metamorphosis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Impact of gestational diabetes and lactational insulin replacement on structure and secretory function of offspring rat ventral prostate

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)