Effects of neem oil (Azadirachta indica A. Juss) on the replacement of the midgut epithelium in the lacewing Ceraeochrysa claveri during larval-pupal metamorphosis


Autoria(s): Scudeler, Elton Luiz; Padovani, Carlos Roberto; Santos, Daniela Carvalho dos
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

18/03/2015

18/03/2015

01/01/2014

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Larvae of the lacewing Ceraeochrysa claveri were fed on eggs of Diatraeasaccharalis treated with neem oil at concentrations of 0.5%, 1% and 2% throughout the larval period. Pupae obtained from treated larvae were used in the study at five days after the completion of cocoon spinning to investigate the effects of neem oil on the replacement of the midgut epithelium during the larval-pupal transition. We observed that the old larval epithelium was shed into the midgut lumen and transformed into the yellow body. Old cells from the yellow body were destroyed by apoptosis and autophagy and were not affected by neem oil. However, neem oil did affect the new pupal epithelium. Cells from treated pupae showed cellular injuries such as a loss of microvilli, cytoplasmic vacuolization, an increase of glycogen stores, deformation of the rough endoplasmic reticulum and dilation of the perinuclear space. Additionally, the neem oil treatment resulted in the release of cytoplasmic protrusions, rupture of the plasma membrane and leakage of cellular debris into the midgut lumen, characteristics of cell death by necrosis. The results indicate that neem oil ingestion affects the replacement of midgut epithelium, causing cytotoxic effects that can alter the organism's physiology due to extensive cellular injuries. (C) 2014 Elsevier GmbH. All rights reserved.

Formato

771-780

Identificador

http://dx.doi.org/10.1016/j.acthis.2014.01.008

Acta Histochemica. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 116, n. 5, p. 771-780, 2014.

0065-1281

http://hdl.handle.net/11449/116400

10.1016/j.acthis.2014.01.008

WOS:000340011900018

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Acta Histochemica

Direitos

closedAccess

Palavras-Chave #Apoptosis #Autophagy #Cellular injury #Digestive system #Lacewing #Necrosis
Tipo

info:eu-repo/semantics/article