Identification of spectral lines of elements using artificial neural networks

Artificial neural networks (ANNs) are relatively new computational tools that have found extensive utilization in solving many complex real-world problems. This paper describes how an ANN can be used to identify the spectral lines of elements. The spectral lines of Cadmium (Cd), Calcium (Ca), Iron (Fe), Lithium (Li), Mercury (Hg), Potassium (K) and Strontium (Sr) in the visible range are chosen for the investigation. One of the unique features of this technique is that it uses the whole spectrum in the visible range instead of individual spectral lines. The spectrum of a sample taken with a spectrometer contains both original peaks and spurious peaks. It is a tedious task to identify these peaks to determine the elements present in the sample. ANNs capability of retrieving original data from noisy spectrum is also explored in this paper. The importance of the need of sufficient data for training ANNs to get accurate results is also emphasized. Two networks are examined: one trained in all spectral lines and other with the persistent lines only. The network trained in all spectral lines is found to be superior in analyzing the spectrum even in a noisy environment.

Theory of differential inequalities with appliciations to singular perturbiation problems and method of lines

During recent years, the theory of differential inequalities has been extensively used to discuss singular perturbation problems and method of lines to partial differential equations. The present thesis deals with some differential inequality theorems and their applications to singularly perturbed initial value problems, boundary value problems for ordinary differential equations in Banach space and initial boundary value problems for parabolic differential equations. The method of lines to parabolic and elliptic differential equations are also dealt The thesis is organised into nine chapters

Threading dislocation lines in two-sided flux-array decorations

Two-sided flux decoration experiments indicate that threading dislocation lines (TDLs), which cross the entire film, are sometimes trapped in metastable states. We calculate the elastic energy associated with the meanderings of a TDL. The TDL behaves as an anisotropic and dispersive string with thermal fluctuations largely along its Burgers vector. These fluctuations also modify the structure factor of the vortex solid. Both effects can, in principle, be used to estimate the elastic moduli of the material.

Establishment of Shrimp Cell Lines: Perception and Orientation

Development of continuous shrimp cell lines for effective investigation on shrimp viruses remains elusive with an arduous history of over 25 years. Despite presenting challenges to researchers in developing a cell line, the billion dollar aquaculture industry is under viral threat. Advances in molecular biology and various gene transfer technologies for immortalization of cells have resulted in the development of hundreds of cell lines from insects and mammals, but yet not a single cell line has been developed from shrimp and other marine invertebrates. Though improved growth and longevity of shrimp cells in vitro could be achieved by using modified growth media this did not make any leap to spontaneous transformation; probably due to the fact that shrimp cells inhibited neoplastic transformations. Oncogenic induction and immortalization are considered as the possible ways, and an exclusive medium for shrimp cell culture and an appropriate mode of transformation are crucial. In this review status of shrimp cell line development and its future orientation are discussed

Studies on arbuscular mycorrhiza (AM) in the Alentejo (Portugal) using pea mutants resistant to AM fungi as a control tool for field conditions

The utilization and management of arbuscular mycorrhiza (AM) symbiosis may improve production and sustainability of the cropping system. For this purpose, native AM fungi (AMF) were sought and tested for their efficiency to increase plant growth by enhanced P uptake and by alleviation of drought stress. Pot experiments with safflower (Carthamus tinctorius) and pea (Pisum sativum) in five soils (mostly sandy loamy Luvisols) and field experiments with peas were carried out during three years at four different sites. Host plants were grown in heated soils inoculated with AMF or the respective heat sterilized inoculum. In the case of peas, mutants resistant to AMF colonization were used as non-mycorrhizal controls. The mycorrhizal impact on yields and its components, transpiration, and P and N uptake was studied in several experiments, partly under varying P and N levels and water supply. Screening of native AMF by most probable number bioassays was not very meaningful. Soil monoliths were placed in the open to simulate field conditions. Inoculation with a native AMF mix improved grain yield, shoot and leaf growth variables as compared to control. Exposed to drought, higher soil water depletion of mycorrhizal plants resulted in a haying-off effect. The growth response to this inoculum could not be significantly reproduced in a subsequent open air pot experiment at two levels of irrigation and P fertilization, however, safflower grew better at higher P and water supply by multiples. The water use efficiency concerning biomass was improved by the AMF inoculum in the two experiments. Transpiration rates were not significantly affected by AM but as a tendency were higher in non-mycorrhizal safflower. A fundamental methodological problem in mycorrhiza field research is providing an appropriate (negative) control for the experimental factor arbuscular mycorrhiza. Soil sterilization or fungicide treatment have undesirable side effects in field and greenhouse settings. Furthermore, artificial rooting, temperature and light conditions in pot experiments may interfere with the interpretation of mycorrhiza effects. Therefore, the myc- pea mutant P2 was tested as a non-mycorrhizal control in a bioassay to evaluate AMF under field conditions in comparison to the symbiotic isogenetic wild type of var. FRISSON as a new integrative approach. However, mutant P2 is also of nod- phenotype and therefore unable to fix N2. A 3-factorial experiment was carried out in a climate chamber at high NPK fertilization to examine the two isolines under non-symbiotic and symbiotic conditions. P2 achieved the same (or higher) biomass as wild type both under good and poor water supply. However, inoculation with the AMF Glomus manihot did not improve plant growth. Differences of grain and straw yields in field trials were large (up to 80 per cent) between those isogenetic pea lines mainly due to higher P uptake under P and water limited conditions. The lacking N2 fixation in mutants was compensated for by high mineral N supply as indicated by the high N status of the pea mutant plants. This finding was corroborated by the results of a major field experiment at three sites with two levels of N fertilization. The higher N rate did not affect grain or straw yields of the non-fixing mutants. Very efficient AMF were detected in a Ferric Luvisol on pasture land as revealed by yield levels of the evaluation crop and by functional vital staining of highly colonized roots. Generally, levels of grain yield were low, at between 40 and 980 kg ha-1. An additional pot trial was carried out to elucidate the strong mycorrhizal effect in the Ferric Luvisol. A triplication of the plant equivalent field P fertilization was necessary to compensate for the mycorrhizal benefit which was with five times higher grain yield very similar to that found in the field experiment. However, the yield differences between the two isolines were not always plausible as the evaluation variable because they were also found in (small) field test trials with apparently sufficient P and N supply and in a soil of almost no AMF potential. This similarly occurred for pea lines of var. SPARKLE and its non-fixing mycorrhizal (E135) and non-symbiotic (R25) isomutants, which were tested in order to exclude experimentally undesirable benefits by N2 fixation. In contrast to var. FRISSON, SPARKLE was not a suitable variety for Mediterranean field conditions. This raises suspicion putative genetic defects other than symbiotic ones may be effective under field conditions, which would conflict with the concept of an appropriate control. It was concluded that AMF resistant plants may help to overcome fundamental problems of present research on arbuscular mycorrhiza, but may create new ones.

Mobiles Lernen

In ihrer Arbeit "Mobiles Lernen. Analyse des Wissenschaftsprozesses der britischen und deutschsprachigen medienpädagogischen und erziehungswissenschaftlichen Mobile-Learning-Diskussion." zeichnet Judith Seipold die Phasen und Entwicklungslinien der bisherigen und naturwüchsigen Diskussion um das Mobile Lernen nach. Dabei eröffnet sie mit ihrer Analyse des vornehmlich britischen Wissenschaftsprozesses des Mobilen Lernens ab Beginn des 21. Jahrhunderts den Blick auf die Struktur der medienpädagogischen und erziehungswissenschaftlichen Mobile-Learning-Diskussion, auf deren Kontexte, Bezugspunkte, Perspektiven und theoretische Schwerpunkte, aber auch Erfolge und Problembereiche in der praktischen Umsetzung des Lernens mit Mobiltechnologien in formalisierten Lernkontexten wie dem des Schulunterrichts. Auf diese Weise liefert die Autorin eine Systematik, die nicht nur die britische Diskussion für die deutschsprachige Medienpädagogik verfügbar macht, sondern auch eine neue und systematisch begründete Reflexionsebene für eine aktuelle medienpädagogische Entwicklung – das Mobile Lernen – bildet. Zunächst widmet sich die Autorin der Analyse des Wissenschaftsprozesses der bisherigen britischen und teils auch der deutschsprachigen Mobile-Learning-Diskussion. Um diesen Prozess in seiner Struktur greifbar zu machen, skizziert sie die Bezugsdisziplinen, aus denen die medienpädagogische und erziehungswissenschaftliche Mobile-Learning-Forschung schöpft, arbeitet die Legitimationsbasis auf, auf die sich Mobiles Lernen stützt, zeichnet die historische Entwicklung der Diskussion nach, die sowohl Alltagsnutzung von Mobiltechnologien als auch den Fachdiskurs einschließt, und erläutert innerhalb ihres Modells der Phasen und Entwicklungslinien Mobilen Lernens Theorien, Konzepte und Modelle, die in der Mobile-Learning-Community als zentral für Analyse und Planung Mobilen Lernens erachtet werden. Konzepte und Modelle, die in der Mobile-Learning-Diskussion die Rolle der Lernenden in das Zentrum der Betrachtungen rücken und eine explizite Handlungsorientierung unterstützen, sind Kernbereich des theoriebasierten Teils der Arbeit. Überlegungen zu einer „Sozio-kulturellen Ökologie Mobilen Lernens“, zu „user-generated contexts“ und zu einer „kulturökologisch informierten Didaktik des Mobilen Lernens“ sind dabei sowohl reflektierende Einordnung aktueller Konzepte und Modelle als auch theoretische und konzeptionelle Basis für die praktische Umsetzung Mobilen Lernens. Um die Rolle der Handlungskompetenzen, kulturellen Praktiken und Strukturen der Lerner bei der Nutzung von Mobiltechnologien für Lernen analytisch zu fassen, finden im Praxis-Kapitel Planungs- und Analyseschemata unter Rückgriff auf Beispiele aus der Praxisforschung und der Implementierung mobilen Lernens in den Schulunterricht Anwendung. Ihre Erörterungen rahmt die Autorin abschließend kritisch und weist zum einen auf bildungspolitische, strukturelle und handlungspraktische Implikationen hin, die sich aus der Mobile-Learning-Diskussion ergeben; zum anderen lenkt sie das Augenmerk auf Gegensätze und Dialektiken des Mobilen Lernens, die sich im Spannungsfeld zwischen alltäglicher mobiler Mediennutzung und der teils theoretisch informierten schulischen Verwendung von Mobiltechnologien zum Lernen entfalten. Sie gilt es, im weiteren Verlauf der Diskussion zu hinterfragen und aufzulösen.

Effects of 17β-estradiol and tamoxifen on the induction of chromosomal abnormalities and on the expression of her2 gene in breast cancer cell lines

This study covers an area of great importance in the research of breast cancer, related to the study of the effects of both estrogens (E2) and anti-estrogens (Tamoxifen) on chromosomes and of modulation of gene expression. Considering that breast cancer is a very heterogeneous disease and that patients respond differently to treatment, the identification of chromosomal abnormalities as well as genes responsive to 17β-estradiol (E2) and Tamoxifen (TAM) could provide the necessary framework to understand the complex effects of this hormone in target cells and could explain, at least in part, the development of cellular resistance to TAM treatment and the subsequent best therapeutic option. In this order of ideas, we determined the effects of E2 and TAM on the chromosomes and on the modulation of gene expression in four breast cancer cell lines, which represent three of the five subtypes of breast cancer known at present. The results are presented in six chapters - each one has a group of the results achieved around the cytogenetic characteristics and gene expression profiles of four cell lines and the effects of E2 and TAM incubation on those. The first chapter describes the main features of breast cancer, furthering the use and effects of E2 and TAM treatment.

Global lines : a citizenship teaching resource for secondary schools.

Con la finalidad de concienciar e involucrar a los estudiantes de secundaria en los problemas mundiales,la Cruz Roja Británica ha desarrollado este recurso que introduce a los alumnos en el concepto de ciudadania del mundo, y les ayuda a comprender mejor el mundo que les rodea y su lugar en él. También, conocen el significado de conceptos como la diversidad, los derechos humanos, la democracia, la justicia social y la participación activa. Las actividades propuestas exploran algunas de las dificultades y los dilemas morales a los que se enfrentan personas en situaciónes violentas y de conflicto, y estudian la respuesta a estas crisis por parte de organizaciones como la Cruz Roja y la Media Luna Roja. Se estudian, en concreto, los casos de Bosnia-Herzegovina, Ruanda e Irlanda del Norte.

Understanding mobile learning : devices, pedagogical implications and research lines

Resumen basado en el de la publicaci??n

The effects of ageing on self-reported aggression measures are partly explained by response bias

Resumen tomado de la publicaci??n

Point sampling with uniformly distributed lines

In this paper we address the problem of extracting representative point samples from polygonal models. The goal of such a sampling algorithm is to find points that are evenly distributed. We propose star-discrepancy as a measure for sampling quality and propose new sampling methods based on global line distributions. We investigate several line generation algorithms including an efficient hardware-based sampling method. Our method contributes to the area of point-based graphics by extracting points that are more evenly distributed than by sampling with current algorithms

A human pancreatic ribonuclease variant kills cancer cells by apoptosis and reduces the expression of P-glycoprotein in MDR cell lines

En aquesta tesi s'han estudiat les propietats antitumorals d'una variant de la ribonucleasa pancreàtica humana anomenada PE5 que incorpora un senyal de localització nuclear. Aquest estudi mostra que PE5 indueix l'apoptosi de les cèl·lules tractades i que aquesta mort és independent de l'activitat de p53. A més, l'efecte citotòxic no es veu afectat per un fenotip de resistència a múltiples drogues. Les dades també mostren que l'activitat citotòxica de PE5 és selectiva per a cèl·lules tumorals in vitro i que la capacitat citotòxica de les dues ribonucleases és semblant. S'ha estudiat l'efecte d'aquestes dues ribonucleases sobre el cicle cel·lular, l'activació de diferents caspases i l'expressió de proteïnes relacionades amb l'apoptosi i el cicle cel·lular. Els resultats indiquen que PE5 i l'onconasa maten les cèl·lules a través de mecanismes diferents. A més, PE5 però no l'onconasa, redueix l'acumulació de glicoproteïna-P en dues línies cel·lulars resistents a múltiples drogues.

Anatomic correlates of strial presbycusis in recombinant inbred mouse strains

This project attempts to identify anatomic features that predict the range of the ‘normal’ endocochlear potential in young inbred mice. Cochlear lateral wall histologic metrics were compared in recombinant inbred (RI) mouse strains formed from BALB/c and C57BL/6 mice.