Minimum volume of long liquid bridges between noncoaxial, nonequal diameter circular disks under lateral acceleration

The stability limit of minimum volume and the breaking dynamics of liquid bridges between nonequal, noncoaxial, circular supporting disks subject to a lateral acceleration were experimentally analyzed by working with liquid bridges of very small dimensions. Experimental results are compared with asymptotic theoretical predictions, with the agreement between experimental results and asymptotic ones being satisfactory

The 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules

In plants, cortical microtubules (MTs) occur in characteristically parallel groups maintained up to one microtubule diameter apart by fine filamentous cross-bridges. However, none of the plant microtubule-associated proteins (MAPs) so far purified accounts for the observed separation between MTs in cells. We previously isolated from carrot cytoskeletons a MAP fraction including 120- and 65-kDa MAPs and have now separated the 65-kDa carrot MAP by sucrose density centrifugation. MAP65 does not induce tubulin polymerization but induces the formation of bundles of parallel MTs in a nucleotide-insensitive manner. The bundling effect is inhibited by porcine MAP2, but, unlike MAP2, MAP65 is heat-labile. In the electron microscope, MAP65 appears as filamentous cross-bridges, maintaining an intermicrotubule spacing of 25–30 nm. Microdensitometer-computer correlation analysis reveals that the cross-bridges are regularly spaced, showing a regular axial spacing that is compatible with a symmetrical helical superlattice for 13 protofilament MTs. Because MAP65 maintains in vitro the inter-MT spacing observed in plants and is shown to decorate cortical MTs, it is proposed that this MAP is important for the organization of the cortical array in vivo.

Alteration of myosin cross bridges by phosphorylation of myosin-binding protein C in cardiac muscle.

In addition to the contractile proteins actin and myosin, contractile filaments of striated muscle contain other proteins that are important for regulating the structure and the interaction of the two force-generating proteins. In the thin filaments, troponin and tropomyosin form a Ca-sensitive trigger that activates normal contraction when intracellular Ca is elevated. In the thick filament, there are several myosin-binding proteins whose functions are unclear. Among these is the myosin-binding protein C (MBP-C). The cardiac isoform contains four phosphorylation sites under the control of cAMP and calmodulin-regulated kinases, whereas the skeletal isoform contains only one such site, suggesting that phosphorylation in cardiac muscle has a specific regulatory function. We isolated natural thick filaments from cardiac muscle and, using electron microscopy and optical diffraction, determined the effect of phosphorylation of MBP-C on cross bridges. The thickness of the filaments that had been treated with protein kinase A was increased where cross bridges were present. No change occurred in the central bare zone that is devoid of cross bridges. The intensity of the reflections along the 43-nm layer line, which is primarily due to the helical array of cross bridges, was increased, and the distance of the first peak reflection from the meridian along the 43-nm layer line was decreased. The results indicate that phosphorylation of MBP-C (i) extends the cross bridges from the backbone of the filament and (ii) increases their degree of order and/or alters their orientation. These changes could alter rate constants for attachment to and detachment from the thin filament and thereby modify force production in activated cardiac muscle.

Equações de quarta ordem na modelagem de oscilações de pontes

Equações diferenciais de quarta ordem aparecem naturalmente na modelagem de oscilações de estruturas elásticas, como aquelas observadas em pontes pênseis. São considerados dois modelos que descrevem as oscilações no tabuleiro de uma ponte. No modelo unidimensional estudamos blow up em espaço finito de soluções de uma classe de equações diferenciais de quarta ordem. Os resultados apresentados solucionam uma conjectura apresentada em [F. Gazzola and R. Pavani. Wide oscillation finite time blow up for solutions to nonlinear fourth order differential equations. Arch. Ration. Mech. Anal., 207(2):717752, 2013] e implicam a não existência de ondas viajantes com baixa velocidade de propagação em uma viga. No modelo bidimensional analisamos uma equação não local para uma placa longa e fina, suportada nas extremidades menores, livre nas demais e sujeita a protensão. Provamos existência e unicidade de solução fraca e estudamos o seu comportamento assintótico sob amortecimento viscoso. Estudamos ainda a estabilidade de modos simples de oscilação, os quais são classificados como longitudinais ou torcionais.

Building bridges��� and properties aplenty: cultural problems in Spanish real estate marketing for prospective British buyers

N.B. reproduced with permission of Peter Lang Verlag. For citation, please, use the original reference, that is Campos Pardillos, M.A. and Balteiro Fernández, I. 2009. “Building bridges��� and properties aplenty: cultural problems in Spanish real estate marketing for prospective British buyers”. In: Guillén-Nieto, V., C. Marimón-Llorca and C. Vargas-Sierra. Eds. Intercultural Business Communication and Simulation and Gaming Methodology. Bern: Peter Lang. Pp. 155-174.

No. 2 College Funds, 1799

One folio-sized leaf containing a handwritten accounting statement of the appropriated and unappropriated income from the Charles River and West Boston bridges and real estate of the College.

Management of floating thrombus in the aortic arch

OBJECTIVE Floating aortic thrombus is an underrecognized source of systemic emboli and carries a life-threatening risk of stroke when located in the aortic arch. Optimal treatment is not established in available guidelines. We report our experience in managing floating thrombi in the aortic arch. METHODS Consecutive patients diagnosed with a floating aortic arch thrombus at a tertiary referral center between January 2008 and December 2014 were reviewed. Perioperative and midterm outcomes were assessed. RESULTS Ten patients (8 female) with a median age of 56 years (range, 47-82 years) were identified. Eight patients presented with a symptomatic embolic event, and 2 patients were asymptomatic. One patient presenting with stroke due to embolic occlusion of all supra-aortic vessels died 2 days after admission. Three patients (2 asymptomatic and 1 unfit for surgery) were treated conservatively by anticoagulation, leading to thrombus resolution in 2 patients. In the third patient, the thrombus persisted despite anticoagulation, resulting in recurrent embolic events. The remaining 6 patients underwent open thrombectomy of the aortic arch during deep hypothermic circulatory arrest. All patients treated by surgery had an uneventful postoperative course with no recurrent thrombus or embolic event during follow-up. Median follow-up of all patients was 17 months (range, 11-89 months). CONCLUSIONS Floating aortic arch thrombus is a dangerous source of systemic emboli. Surgical removal of the thrombus is easy to perform and followed by good clinical results. Conservative treatment with anticoagulation may be considered in asymptomatic, inoperable or high-risk patients.