Cell-free activation of neutrophil NADPH oxidase by a phosphatidic acid-regulated protein kinase.

The phosphorylation-dependent mechanisms regulating activation of the human neutrophil respiratory-burst enzyme, NADPH oxidase, have not been elucidated. We have shown that phosphatidic acid (PA) and diacylglycerol (DG), products of phospholipase activation, synergize to activate NADPH oxidase in a cell-free system. We now report that activation by PA plus DG involves protein kinase activity, unlike other cell-free system activators. NADPH oxidase activation by PA plus DG is reduced approximately 70% by several protein kinase inhibitors [1-(5-isoquinolinesulfonyl)piperazine, staurosporine, GF-109203X]. Similarly, depletion of ATP by dialysis reduces PA plus DG-mediated NADPH oxidase activation by approximately 70%. Addition of ATP, but not a nonhydrolyzable ATP analog, to the dialyzed system restores activation levels to normal. In contrast, these treatments have little effect on NADPH oxidase activation by arachidonic acid or SDS plus DG. PA plus DG induces the phosphorylation of a number of endogenous proteins. Phosphorylation is largely mediated by PA, not DG. A predominant substrate is p47-phox, a phosphoprotein component of NADPH oxidase. Phosphorylation of p47-phox precedes activation of NADPH oxidase and is markedly reduced by the protein kinase inhibitors. In contrast, arachidonic acid alone or SDS plus DG is a poor activator of protein phosphorylation in the cell-free system. Thus, PA induces activation of one or more protein kinases that regulate NADPH oxidase activation in a cell-free system. This cell-free system will be useful for identifying a functionally important PA-activated protein kinase(s) and for dissecting the phosphorylation-dependent mechanisms responsible for NADPH oxidase activation.

La evolución de los periódicos digitales frente a los convencionales como soporte publicitario

Research performed in this thesis studies the growth and evolution of advertising investment in digital media compared to traditional printed media, in particular daily newspapers. By means of advertising inserts in printed newspaper and online newspapers, the study’s objective was to confirm that digital media consolidate as an advertising platform. Throughout this study we used different quantitative and qualitative tools to demonstrate the starting hypothesis of this thesis. We used a combined methodology that included: a) joint analysis of printed newspaper “El Mundo” and online newspaper “elmundo.es” advertising content; b) analysis of advertising investment state-of-the-art; c) deep interviews to different media professionals, including advertisers, publicists, media planning, and researchers. A comparative study between the printed newspaper “El Mundo” and the online newspaper “elmundo.es” supported the analysis of contents in a realistic situation. Therefore a deep study of advertising inserts in both the printed and online version of “El Mundo” was performed. The main goal of this work was to show how advertising invesment is moving from traditional printed media to digital media The results of this study reveal that investment in digital media increases, meanwhile investment reduces in traditional media. At the same time, new reading habits along with technological innovation attracts readers to digital platforms. As a consequence, traditional printed newspapers have the immediate need to somehow engage online-newspaper readers into printed platforms. Hence, many advertisers already invest large sums of money in digital media, with marketing strategies and plans focused to online advertising campaigns in Internet. It is also true that in recent years printed and online media stopped “fighting”, which lead to the possibility of joint marketing and communication strategies between the aforementioned digital and online media. This research has different implications, one of them is that printed media seems to be a bussiness model which needs to be reinvented to adapt itself to the media’s current situation in order to be profitable and not to disappear. On the other hand, digital media must have a well-defined strategy with concrete and viable goals in online investment. Finally, it is important that new readers are taken into account by newspapers, this is: new readers mostly use digital platforms, thus interaction between readers and newspapers is much different than what it used to be when only printed newspapers existed. Media must go and be where the new reader is.

The Psychology of Adolescent Out-of Wedlock Pregnancy and Childbearing

Illegitimate adolescent pregnancy creates a variety of problems, beginning with the difficult decision about whether or not to terminate the pregnancy. If the pregnancy is carried to term, choices follow regarding marriage or single parenthood and keeping or relinquishing the child. All of these choices involve consequences for the adolescent, many of them negative ones. This paper examines the problem of out-of-wedlock teen pregnancy and its possible psychological sources. It also introduces a method for analyzing the psychology of unwed teen pregnancy and childbearing and reviews the literature on the subject by this method. NOTE: Approvals page submitted to digital archive lacks signatures

Prática de ensino supervisionada em Educação Pré-escolar e Ensino do 1.º Ciclo do Ensino Básico

O presente relatório foi realizado no âmbito da unidade curricular da Prática de Ensino Supervisionada (PES), inserida no plano de estudos do curso de Mestrado em Educação Pré-Escolar e Ensino do 1.º Ciclo do Ensino Básico, da Escola Superior de Educação de Bragança, do Instituto Politécnico de Bragança. Com este relatório, pretendemos apresentar as experiências de ensino aprendizagem que consideramos relevantes e representativas do trabalho desenvolvido com as crianças ao longo da nossa PES em ambos os contextos educativos. Estas reportam-se à descrição, reflexão e investigação acerca da ação educativa. A prática pedagógica foi desenvolvida na Educação Pré-Escolar (EPE), com um grupo de 25 crianças, com idades de 3 e 4 anos e, no 1.º Ciclo do Ensino Básico (1.º CEB), com um grupo de 10 crianças pertencentes ao 1.º ano de escolaridade. O contexto na Educação Pré-Escolar caraterizava-se por ser uma Instituição Particular de Solidariedade Social (IPSS) e o contexto de 1.º Ciclo do Ensino Básico pertencia à rede pública. No decorrer da ação educativa, tivemos em conta a articulação curricular, onde mantivemos sempre presente as necessidades das crianças, os seus interesses e ritmos de aprendizagem. Para tal, apoiamo-nos nos documentos oficiais e orientadores da prática pedagógica, sendo estes as Orientações Curriculares para a Educação Pré-Escolar e o Programa Nacional do 1.º ano do 1.º Ciclo do Ensino Básico, indo ao encontro da questão problema: De que forma os diferentes suportes (papel/digital) motivam as crianças no seu processo de leitura em contexto jardim de infância e no 1.º Ciclo do Ensino Básico? Para dar resposta delineamos os seguintes objetivos: (i) Perceber se o tipo de suporte em que as crianças efetuam as suas leituras influencia a sua motivação; (ii) Perceber se a leitura em suporte digital contribui para o desenvolvimento do gosto pela leitura; (iii) Verificar se a leitura em suporte papel permite uma maior motivação na criança em relação ao suporte digital. Os dados foram recolhidos no decorrer das intervenções, através da observação, com recurso às notas de campo, ao registo fotográfico e ao questionário. A apresentação das experiências de ensino/aprendizagem presentes neste relatório traduzem-se num processo descritivo, interpretativo e reflexivo, enquadrado numa abordagem qualitativa. É de salientar que ao longo da nossa prática educativa adotamos uma atitude reflexiva e crítica face ao nosso trabalho, tornando-nos observadores ativos neste processo, assumindo ao mesmo tempo, o papel de investigadoras.

Encadrement juridique des monnaies numériques : bitcoin et autres cryptomonnaies

Les informations relatives aux cryptomonnaies sont susceptibles de changer à l'avenir tant cette matière est nouvelle et encore peu ancrée dans le droit. Ce mémoire est une réflexion sur l'essor du Bitcoin et des cryptomonnaies à leurs débuts, alors même que le droit cherche à s'accaparer ces nouvelles technologies, à les intégrer dans son système préexistant.

Encadrement juridique des monnaies numériques : bitcoin et autres cryptomonnaies

Les informations relatives aux cryptomonnaies sont susceptibles de changer à l'avenir tant cette matière est nouvelle et encore peu ancrée dans le droit. Ce mémoire est une réflexion sur l'essor du Bitcoin et des cryptomonnaies à leurs débuts, alors même que le droit cherche à s'accaparer ces nouvelles technologies, à les intégrer dans son système préexistant.

Music Notes: Exploring Music Listening Data as a Visual Representation of Self

Thesis (Master's)--University of Washington, 2016-06

Phase noise influence in coherent optical DnPSK systems with DSP based dispersion compensation

We present a comparative study of the influence of dispersion induced phase noise for n-level PSK systems. From the analysis, we conclude that the phase noise influence for classical homodyne/heterodyne PSK systems is entirely determined by the modulation complexity (expressed in terms of constellation diagram) and the analogue demodulation format. On the other hand, the use of digital signal processing (DSP) in homodyne/intradyne systems renders a fiber length dependence originating from the generation of equalization enhanced phase noise. For future high capacity systems, high constellations must be used in order to lower the symbol rate to practically manageable speeds, and this fact puts severe requirements to the signal and local oscillator (LO) linewidths. Our results for the bit-error-rate (BER) floor caused by the phase noise influence in the case of QPSK, 16PSK and 64PSK systems outline tolerance limitations for the LO performance: 5 MHz linewidth (at 3-dB level) for 100 Gbit/s QPSK; 1 MHz for 400 Gbit/s QPSK; 0.1 MHz for 400 Gbit/s 16PSK and 1 Tbit/s 64PSK systems. This defines design constrains for the phase noise impact in distributed-feed-back (DFB) or distributed-Bragg-reflector (DBR) semiconductor lasers, that would allow moving the system capacity from 100 Gbit/s system capacity to 400 Gbit/s in 3 years (1 Tbit/s in 5 years). It is imperative at the same time to increase the analogue to digital conversion (ADC) speed such that the single quadrature symbol rate goes from today's 25 GS/s to 100 GS/s (using two samples per symbol). © 2014 by Walter de Gruyter Berlin/Boston.

Guest editorial:special issue on digitial enterprise technology

Full text This Proceedings volume contains selected papers from the Fourth International CIRP-sponsored, Conference on Digital Enterprise Technology (DET2007), which was held at the University of Bath, UK, 19–21 September 2007. All selected papers have been suitably enhanced for publication in the Journal and have undergone full review. Digital enterprise technology (DET) is ‘the collection of systems and methods for the digital modelling and analysis of the global product development and realization process, in the context of lifecycle management.’ The principal aim of the DET concept is to provide a coherent context for the development and integration of the various digital technologies that underpin modern design and manufacturing. These technologies can be classified according to the following five key areas. 1. Distributed and collaborative design. 2. Process modelling and process planning. 3. Advanced factory design and modelling. 4. Physical-to-digital environment integrators–verification. 5. Enterprise integration technologies. This special issue is representative of the wide breadth of the DET concept including; a comprehensive review of digital engineering, design processes, digital modelling of machine tools, forming, robotics and machining processes, verification and metrology, and dynamic networks. It is particularly pleasing to see the development of metrology as a key aspect of modern manufacturing technology, linking design intent to process capability. The papers published herein will facilitate the exploration of new and evolving research concepts by the international research community and will influence the development of international standards for the application of DET technologies.

Análise geoambiental da plataforma continental Rasa Equatorial, por meio de sensoriamento remoto e dados sedimentológicos: parrachos de Rio do Fogo-RN, Brasil

In the shallow continental shelf in Northeastern Rio Grande do Norte - Brazil, important underwater geomorphological features can be found 6km from the coastline. They are coral reefs, locally known as “parrachos”. The present study aims to characterize and analyze the geomorphological feature as well as the ones of the benthic surface, and the distribution of biogenic sediments found in parrachos at Rio do Fogo and associated shallow platforms, by using remote sensing products and in situ data collections. This was made possible due to sedimentological, bathymetric and geomorphological maps elaborated from composite bands of images from the satellite sensors ETM+/Landsat-7, OLI/Landsat-8, MS/GeoEye and PAN/WordView-1, and analysis of bottom sediments samples. These maps were analyzed, integrally interpreted and validated in fieldwork, thus permitting the generation of a new geomorphological zoning of the shallow shelf in study and a geoenvironmental map of the Parrachos in Rio do Fogo. The images used were subject to Digital Image Processing techniques. All obtained data and information were stored in a Geographic Information System (GIS) and can become available to the scientific community. This shallow platform has a carbonate bottom composed mostly by algae. Collected and analyzed sediment samples can be classified as biogenic carbonatic sands, as they are composed 75% by calcareous algae, according to the found samples. The most abundant classes are green algae, red algae, nonbiogenic sediments (mineral grains), ancient algae and molluscs. At the parrachos the following was mapped: Barreta Channel, intertidal reefs, submerged reefs, the spur and grooves, the pools, the sandy bank, the bank of algae, sea grass, submerged roads and Rio do Fogo Channel. This work presents new information about geomorphology and evolution in the study area, and will be guiding future decision making in the handling and environmental management of the region

Personal learning artefacts: persistence, ownership and privacy

Traditional classrooms have been often regarded as closed spaces within which experimentation, discussion and exploration of ideas occur. Professors have been used to being able to express ideas frankly, and occasionally rashly while discussions are ephemeral and conventional student work is submitted, graded and often shredded. However, digital tools have transformed the nature of privacy. As we move towards the creation of life-long archives of our personal learning, we collect material created in various 'classrooms'. Some of these are public, and open, but others were created within 'circles of trust' with expectations of privacy and anonymity by learners. Taking the Creative Commons license as a starting point, this paper looks at what rights and expectations of privacy exist in learning environments? What methods might we use to define a 'privacy license' for learning? How should the privacy rights of learners be balanced with the need to encourage open learning and with the creation of eportfolios as evidence of learning? How might we define different learning spaces and the privacy rights associated with them? Which class activities are 'private' and closed to the class, which are open and what lies between? A limited set of set of metrics or zones is proposed, along the axes of private-public, anonymous-attributable and non-commercial-commercial to define learning spaces and the digital footprints created within them. The application of these not only to the artefacts which reflect learning, but to the learning spaces, and indeed to digital media more broadly are explored. The possibility that these might inform not only teaching practice but also grading rubrics in disciplines where public engagement is required will also be explored, along with the need for consideration by educational institutions of the data rights of students.

Into the Bends of Time and Musical Forces in Jazz: Group Interaction and Double-time in “My Foolish Heart” as performed by the Bill Evans Trio with Scott LaFaro and Paul Motian.

Into the Bends of Time is a 40-minute work in seven movements for a large chamber orchestra with electronics, utilizing real-time computer-assisted processing of music performed by live musicians. The piece explores various combinations of interactive relationships between players and electronics, ranging from relatively basic processing effects to musical gestures achieved through stages of computer analysis, in which resulting sounds are crafted according to parameters of the incoming musical material. Additionally, some elements of interaction are multi-dimensional, in that they rely on the participation of two or more performers fulfilling distinct roles in the interactive process with the computer in order to generate musical material. Through processes of controlled randomness, several electronic effects induce elements of chance into their realization so that no two performances of this work are exactly alike. The piece gets its name from the notion that real-time computer-assisted processing, in which sound pressure waves are transduced into electrical energy, converted to digital data, artfully modified, converted back into electrical energy and transduced into sound waves, represents a “bending” of time.

The Bill Evans Trio featuring bassist Scott LaFaro and drummer Paul Motian is widely regarded as one of the most important and influential piano trios in the history of jazz, lauded for its unparalleled level of group interaction. Most analyses of Bill Evans’ recordings, however, focus on his playing alone and fail to take group interaction into account. This paper examines one performance in particular, of Victor Young’s “My Foolish Heart” as recorded in a live performance by the Bill Evans Trio in 1961. In Part One, I discuss Steve Larson’s theory of musical forces (expanded by Robert S. Hatten) and its applicability to jazz performance. I examine other recordings of ballads by this same trio in order to draw observations about normative ballad performance practice. I discuss meter and phrase structure and show how the relationship between the two is fixed in a formal structure of repeated choruses. I then develop a model of perpetual motion based on the musical forces inherent in this structure. In Part Two, I offer a full transcription and close analysis of “My Foolish Heart,” showing how elements of group interaction work with and against the musical forces inherent in the model of perpetual motion to achieve an unconventional, dynamic use of double-time. I explore the concept of a unified agential persona and discuss its role in imparting the song’s inherent rhetorical tension to the instrumental musical discourse.