993 resultados para 1,15-Alkanediol C30


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为了揭示不同类型植被下土壤有机碳及其活性组分季节动态变化及其特点,探讨不同的植被恢复模式对土壤有机碳组分的影响,分析影响土壤有机碳组分变化的因素,评估土壤有机活性有机碳组分参数在植被恢复过程中土壤质量监测的可靠性,为植被恢复及低效林改造技术提供理论依据。本研究选择岷江上游大沟流域的几种人工林(云杉林、油松林、华山松林、日本落叶松林)以及次生落叶阔叶灌丛下土壤,通过剖面机械分层取样,测定土壤总有机碳(TOC)和三种活性碳组分微生物碳(SMBC)、水溶性碳(WSOC)、易氧化碳(EOC)等来反映土壤变化特点。主要结果是: 1. 土壤有机碳含量平均在15.48~25.46 g kg-1之间在5月份时含量最低,随生长季的开始,有机碳含量逐渐增加,到9月份时含量达到最大值;由于新形成的凋落物不能被迅速分解利用补充土壤碳库,而原有碳库经历一个生长季的分解利用,因此,生长季末期即11月份的含量较小;土壤微生物碳含量平均在132.78~476.73mg kg-1之间,9月份和11月份含量都比较高;水溶性碳在生长季中逐步增大,含量在51.95~77.18 mg kg-1之间,到11月份时达到最大值;土壤易氧化碳平均含量在3.74~5.79g kg-1之间,含量最低值出现在5月份,但和其他碳组分不同的是其在7月份时含量较高。 2. 土壤有机碳及其活性碳组分大小关系为:TOC>EOC >SMBC>WSOC;比值约为300:70:5:1。 3. 土壤不同层次间比较,土壤碳指标都表现为随土壤深度增加而逐渐减小, 表层积聚作用明显。 4. 对土壤总有机碳量与活性碳组分以及活性碳之间进行了相关分析表明,土壤总有机碳含量与土壤微生物量碳、水溶性碳、易氧化碳之间的相关性均达到显著水平(P<0.05),有机碳总贮量很大程度上制约着土壤活性碳组分。土壤微生物量碳、水溶性碳、易氧化碳两两之间也都存在着显著相关关系(P<0.05),并随着不同植被类型或立地条件因子发生变化而变化。 5. 土壤有机碳及其活性组分与土壤养分状况之间的相关性分析发现,随着海拔、坡向或者植被类型的改变,其林下土壤有机碳及其活性组分与土壤养分的相关性也发生较大的变化。总体而言,岷江上游地区海拔、坡向、土壤自然含水量、植被盖度、凋落物厚度、土壤全N对次生林下土壤有机碳及其组分有重要影响。而AP、AK、C/N对土壤碳变化变化影响较小。 6. 通过不同海拔、坡向以及植被类型之间的综合比较分析发现,土壤微生物碳SMBC和水溶性碳WSOC比TOC和EOC更能敏感地反映出比较敏感的指示林下土壤质量的变化。 In order to reveal seasonal dynamics of soil labile organic carbon under different secondary vegetation, to analyze effect of different vegetation restoration pattern on soil organic carbon and its fractions, and to find the factors influencing changes in soil organic carbon and its fractions, further to estimate those parameters reliability for soil quality monitoring in the process of vegetation restoration. Soils were selected from several plantations, including Picea asperata Pinus tabulaeformis, Pinus armandii and Larix kaempferi and secondary shrub in Dagou Watershed of the upper reach of Minjiang River. The measurement of TOC, SMBC,WSOC and EOC were made, because these parameters can reflect change of soil characteristics. The major results are: 1. There were the lowest soil organic carbon and its labile fractions contents in May. At the time of growth initiation, they increased gradually and reached maximum in September. After that the soil organic carbon content decreased. Because current litter couldn’t be rapidly decomposed, and supplemented into carbon pool, while intrinsic carbon pool experienced decomposition and utilization of growth season, Which led a decrease in soil organic carbon content in November. Average value was 15.48~25.46 g kg-1; average SMBC content was 132.78~476.73mg kg-1.There were higher SMBC content in September and November as compared with other times; Water soluble organic carbon content increased from 51.95 mg kg-1 in May to 77.18 mg kg-1 in November; EOC content was lowest in May y. Average value was 3.74~5.79g kg-1. Differeing from other parameters of carbon fractions, EOC content was higher in July. 2. The content of soil organic carbon and its labile carbon fractions ranked as follows:TOC>EOC >SMBC>WSO,and ratio was about 300:70:5:1. 3. Consider as soil different layers,all of the parameters decreased gradually with increasing soil depth, thus displayed a significant accumulation in the surface layer soil. 4. Correlations coefficient analysis revealed that, TOC significantly correlated with SMBC, WSOC and EOC indicating total storage of organic carbon limited soil labile carbon fractions in great extent. On the other hand, there were significant correlations between SMBC,WSOC and EOC. But these relationships changed with vegetation types and/or environmental conditions. 5. The relationships between soil organic carbon and its labile fractions and soil nutrient traits changed with altitude,slope aspect and vegetations. Therefore our results suggested that altitude,slope aspect,soil natural water content,vegetation coverage, litter thickness and soil total nitrogen play a important role change in soil organic carbon and its fractions in upper reaches of Minjiang River. While AP、AK、C/N slightly influenced soil carbon. 6. Our results, on the other hand suggested that SMBC and WSOC are more sensitive to the change of altitudes, slope aspects, vegetation types than TOC and EOC, thus two parameters may be good index reflecting change of soil quality. These results provide insights into theoretical and technological evidences for the vegetation regeneration restoration and improvement of low-quality and benefit forest in the upper reaches of Minjiang River regions.

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糯小麦在食品加工业、淀粉加工业及其它工业上有着重要用途,是近年来许多国家小麦研究的重要课题。国外糯小麦选育尚未突破高产与糯性相结合的难点,国内目前还没有培育出高蛋白强筋型的糯小麦品种,这在一定程度上与缺乏合适的育种方法和高效、实用的糯小麦分子标记辅助育种技术有关。国内外对Wx基因效应的研究主要利用缺体-四体系、重组自交系或近等基因系,还未见有利用遗传背景相同的BC5F2代回交改良群体的报道。 糯性位点近等基因系是小麦淀粉品质育种的重要材料,而我国目前还没有一套中国栽培小麦遗传背景的糯性位点近等基因系。为了选育部分糯小麦、全糯小麦和中国栽培小麦遗传背景的糯性位点近等基因系,我们利用Wx蛋白电泳和高效实用的分子标记技术体系来鉴定糯小麦杂交后代的基因型,结果证明该体系能有效地用于糯小麦的分子标记辅助育种。以中国春糯性位点全套近等基因系为研究材料,对小麦Wx基因的6个STS标记和1个CAPS标记进行了筛选,改良PCR扩增条件以及产物检测方式后,从这些标记中筛选出3个标记,包括鉴定Wx-A1、Wx-D1位点的2个共显性STS标记和Wx-B1位点的1个显性STS标记。利用上述3个分子标记从BC5F2 代回交改良群体中筛选出了8种Wx基因型,经卡方检验,其分离比符合3对基因的分离比例,其中基因型为aabbdd的植株有2株,直链淀粉含量分别为1.81%和0.82%,为全糯小麦;基因型为AAbbdd,aabbDD的部分糯性植株各有1株,直链淀粉含量分别为15.24%和17.57%。以上4株植株的农艺性状和品质性状接近回交亲本“川育12”,并明显优于全糯材料“98Y1441”,表明采用回交法与Wx基因分子标记辅助选择相结合,有助于培育高产、优质的全糯和部分糯小麦。同时,本研究中建立的分子标记技术体系,也为选育具有中国栽培小麦遗传背景的糯性位点近等基因系奠定了基础。 在基因型鉴定的基础上,利用糯小麦杂交后代BC5F2代回交改良群体研究了各基因缺失降低直链淀粉含量的效果和各基因合成直链淀粉的能力,以及直链淀粉含量与农艺性状、品质性状、淀粉糊化特性等的相关性。缺失不同Wx基因的8种基因型,直链淀粉含量差异显著。研究单缺失基因型发现,减少效应最大的是Wx-B1b基因,Wx-B1b和Wx-D1b基因没有显著差异,减少效应最小的是Wx-A1b基因。研究双缺失基因型发现,Wx基因合成直链淀粉的能力,Wx-B1a基因最高,Wx-A1a基因最低,而Wx-B1a和Wx-D1a基因差异很小;直链淀粉含量与株高、穗长、小穗数、穗粒数、千粒重等农艺性状相关不显著,表明淀粉品质育种可以与高产育种实现有机结合;直链淀粉含量与SDS-沉降值呈显著负相关(r=-0.726),说明直链淀粉含量降低在一定程度上有利于提高小麦营养与加工品质,这一结果至今未见有文献报道;全糯类型的淀粉糊化特性与其他类型显著不同,具有最高的峰值粘度和稀懈值,最低的低谷粘度、最终粘度、反弹值、峰值时间、糊化温度、起始糊化温度,表明糯小麦淀粉在食品和工业上具有特殊用途;稀懈值与直链淀粉含量呈极显著负相关(r=-0.969),其他粘度参数与直链淀粉含量呈显著正相关(最终粘度r=0.797,低谷粘度r=0.910、反弹值r=0.954、峰值时间r=0.970、糊化温度r=0.962、起始糊化温度r=0.932)。以上结论可为糯小麦品种选育和淀粉品质改良提供理论依据。 Waxy wheat is very important in food processing industry, starch processing industry and the other industries, so it is a focus of wheat research in many countries these years. Foreign wheat breeders have not conquered the difficulty of high yield combined with waxy character, and there is no waxy wheat variety with high protein and strong gluten in China at present,all of which were caused by lacking proper breeding methods and effective, applied molecular markers-assisted selection technique at a certain extent. Until now, research about the effect of waxy genes mainly depended on nullisomic-tetrasomic lines, recombinant lines or near-isogenic lines, and it is lacking the reports of using improved BC5F2 backcross progenies under the common genetic background. Near-isogenic lines at the Wx loci are important materials for wheat starch quality breeding. However, there are no such lines under the Chinese cultivated wheat genetic background. To develop partial waxy wheats, waxy wheats and near-isogenic lines at the Wx loci under the Chinese cultivated wheat genetic background, we use SDS-PAGE of waxy proteins and effective, applied molecular marker-assisted selection technical system to identify the genotype of waxy wheat’s progenies. The results indicated that such a system is applicable in waxy wheat’s molecular marker-assisted selection effectively. A series of Chinese Spring Wx loci near-isogenic lines were used to identify the specific bands of six STS markers and one CAPS marker of Wx genes. After optimizing PCR amplification and separating of PCR products, three co-dominant and dominant STS-markers were identified at the Wx-A1, Wx-D1 and Wx-B1 loci, respectively, which were used to identify the genotype of waxy wheat’s progenies. Eight Wx genotypes were developed from the improved BC5F2 backcross progenies, which follows Mendelian segregation. Among them, there were two aabbdd waxy plants whose amylose content in the BC5F3 seeds were 1.81% and 0.82%, respectively. In addition, there were partial waxy plants (AAbbdd and aabbDD) whose amylose content in the BC5F3 seeds were 15.24 % and 17.57%, respectively. Most agronomic and quality traits of these four plants resembled those of the recurrent parent “Chuanyu 12”, and superior to waxy wheat parent “98Y1441”. This shows that backcross approach in combination with molecular marker-assisted selection of waxy genes is helpful to develop partial and full waxy wheat with good traits in the waxy wheat breeding program. At the same time, molecular marker-assisted selection technical system in this paper, also establish the base for breeding the near-isogenic lines at the Wx loci under the Chinese cultivated wheat genetic background. According to the results of genotype identification, we use waxy wheat’s improved BC5F2 backcross progenies to verify the effects of null alleles on reducing amylose content and determine the amylose synthesis capacity of each Wx gene independently, and investigate the relativity among amylose content with agonomic traits, quality traits, starch pasting properties respectively. There was significant difference in amylose content of the eight genotypes carrying different null Wx alleles. The reducing effect of the single null alleles was the most significant in Wx-B1b, followed by Wx-D1b and Wx-A1b, and there was no significant difference between Wx-B1b and Wx-D1b. The results of the double null lines further demonstrated that for the capacity of amylose synthesis, Wx-B1a was the highest, followed by Wx-D1a and Wx-A1a, and there was no significant difference between Wx-B1a and Wx-D1a. Amylose contents of the eight genotypes were not significantly correlated with plant height, spike length, spikelets per spike, grains per spike, 1000-grain weight, which showed that starch quality breeding could integrate with high yield breeding. Amylose contents of the eight genotypes were negatively significantly correlated with SDS-sedimentation value(r=-0.726), which suggested that reduction in amylose content is propitious to improve quality at a certain extent. Starch pasting properties of the full waxy type was significantly different from the other seven types, with the highest peak viscosity and breakdown, and lowest valley viscosity, final viscosity, setback, peak time, pasting temperature and starting pasting temperature. It indicated that waxy wheat starch has special use in food and industry. Breakdown was negatively significantly correlated with amylose contents (r=-0.969), and the other parameters were positively significantly correlated with amylose contents (r=0.797 for final viscosity, r=0.910 for trough viscosity, r =0.954 for setback, r =0.970 for peak time, r=0.962 for pasting temperature and r=0.932 for starting pasting temperature ). The results of the study are very useful for waxy wheat variety breeding and starch quality improvement.

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对15株白腐真菌进行了以玉米秸秆为基质的初步筛选,从中获得一株选择性系数较高的菌株Y10,并对其降解玉米秸秆的情况进行了研究。结果表明,在30天的培养过程中菌株Y10对玉米秸秆降解的选择性系数都大于1,第15天选择性系数最高为3.88。对未经降解和降解过的玉米秸秆分别作了紫外光谱和红外光谱分析,结果表明,经该菌降解后玉米秸秆的化学成分发生了很大变化,且木质素的降解程度要大于纤维素的降解程度。对菌株Y10进行了ITS-5.8S rDNA序列鉴定,初步判定其为Cerrena sp.。 为了考查不同的外源添加物对菌株Y10降解玉米秸秆的影响,在以玉米秸秆为基质的固态发酵培养基中分别添加了7种金属离子、8种碳源、6种氮源。结果显示,这7种金属离子均能促进木质素的降解,并且一定浓度的某些离子明显抑制纤维素的降解;其中添加0.036%的MnSO4·H2O和0.36%的MgSO4·7H2O对纤维素降解的抑制作用比较强,降解率分别为0.96%和1.31%,木质素的选择性系数分别达到了34.40和20.17。8种碳源中除麦芽糖外都能促进木质素的降解,除微晶纤维素外都明显促进纤维素的降解。6种氮源中酒石酸铵、硫酸铵、草酸铵和氯化铵的添加都会使该菌生长变慢,而且氮源浓度越高菌丝生长越慢。外加碳源和金属离子对半纤维素降解和选择性系数的影响不大。 同时对菌株Y10在液态培养下产木质素降解酶的条件和培养基做了优化。结果表明,在初始产酶培养基中,菌株Y10的漆酶酶活在第10d达到最高,锰过氧化物酶酶活在第11d达到最高,基本上检测不到木质素过氧化物酶。菌株Y10产漆酶的最适温度为32℃,最适PH为6.0;产锰过氧化物酶的最适温度为32℃,最适PH为6.5。菌株Y10产漆酶的最佳碳源为甘露糖,最佳氮源为酒石酸铵,最适诱导剂VA浓度为3 mmol/L,最适表面活性剂TW-80浓度为1%。 利用响应面法对其产漆酶的培养基进行优化,优化后的培养基配方为葡萄糖10.00 g/L,酒石酸铵0.50 g/L,大量元素296.50 ml/L,微量元素100.00 ml/L,NTA 1.40 g/L,VA 5.00 mmol/L,吐温-80加入量为0.10%。进行了菌株Y10产漆酶的验证实验,实测酶活为5282.56 U/L,与预测酶活5162.73 U/L接近。在优化后培养基中,菌株Y10在第14 d达到生长的最高峰,第20 d时,漆酶酶活最高,为11325.00 U/L;第16 d时,锰过氧化物酶酶活最高,为30.77 U/L。 对菌株Y10的漆酶酶学性质做了初步的研究,结果显示,酶反应的最适温度为40℃-65℃,最适PH为3.0。在40℃,PH=3.0时,漆酶催化ABTS反应的米氏方程为 。 Fifteen white-rot fungi based on corn stalk were screened. One white-rot fungus Y10 with high selectivity value was obtained. The degradation of corn stalk was initially studied. The results indicated that the selectivity value was above 1 during the 30 day-cultivation and the highest was 3.88 after 15 days. The composition of untreated and treated stalk was analyzed through ultraviolet spectroscopy and infrared spectroscopy. It was found that the composition of treated stalk was greatly altered and the degree of the degradation of lignin is greater than the cellulose. Y10 was identified as Cerrena sp. by ITS -5.8S rDNA sequence analysis. The influence of metal ions, carbon sources and nitrogen sources on corn stalk degradation by white-rot fungus was studied. While all seven metal ions could promote lignin degradation, the cellulose degradation was best inhibited at certain ion concentrations. Notably, when 0.036% MnSO4·H2O and 0.36% MgSO4·7H2O were added into the medium, the cellulose degradation was restrained to the extents that the coefficients of lignin selectivity rose to 34.40 and 20.17 respectively. It was also found that all carbon sources except maltose can promote lignin degradation. The addition of carbon sources other than microcrystalline cellulose significantly promoted cellulose degradation. The addition of the nitrogen sources, ammonium tartrate, ammonium sulfate, oxalate, ammonium chloride, resulted in remarkable inhibition to mycelium growth; the larger the concentrations of nitrogen sources are, the slower the mycelium grew. The addition of carbon sources and metal ions had less impact on the degradation of hemicellulose and selectivity value. Meanwhile, we optimized the conditions and culture medium of the lignin-degrading enzyme production of strain Y10. The results showed that in the initial culture medium, the Lac activity was highest at the 10th day, the MnP activity was highest at the 11th day and the LiP could not be detected. The optimum condition of Lac was at temperature 32 and PH =6.0 and the optimum condition of MnP was at temperature 32 and PH =6.5. The optimum carbon source for Lac was seminose, the optimum nitrogen source was ammonium tartrate, the optimum content of VA was 3 mmol/L, the optimum content of TW-80 was 1%. PB and RSM were used to optimize the culture medium of laccase by white-rot fungus Y10. The optimum culture medium was consist of glucose 10.00 g/L, ammonium tartrate 0.50 g/L, macro elements 296.50 ml/L, trace elements 100.00 ml/L, NTA 1.40 g/L, VA 5.00 mmol/L, TW-80 0.10%. Under the optimal conditions, the activity of laccase was 5282.56 U/L and the experimental value agreed with the predicted value 5162.73 U/L. The biomass was highest at the 14th day, the Lac activity was highest at the 20th day, the MnP activity was highest at the 16th day. The results of the studies on the characteristics of Lac showed that the optimum temperature for Lac activity is 40℃-65℃ ; the optimum PH for Lac activity is 3.0 and under 40℃,PH=3.0, the Michaelis-menten equation of Lac catalized ABTS oxidation was .

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对当前国际上超重元素化学性质实验研究的现状进行了综述,简单介绍了超重元素的合成方法和开展超重元素化学实验的技术路线。综述了超重元素Rf,Db,Sg的液相化学性质研究及Rf,Db,Sg,Bh,Hs和112号元素的气相化学性质研究的实验进展情况。最后介绍了我国在超重新核素合成方面所取得的成果,并展望了在超重元素化学性质研究方面的前景。

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为了描述快重离子在聚合物中的潜径迹行为 ,用不同能量的快重离子 (1 15 8GeVFe56 ,1 75 5GeVXe1 36 及 2 6 36GeVU2 38)辐照叠层半结晶聚碳酸酯膜 (MakrofolKG型 ) ,结合x射线衍射测量技术 ,在较宽的电子能损 (1 9— 17 1keV nm)和离子注量 (5× 10 1 0 — 3× 10 1 2 cm- 2 )范围研究了离子在半结晶聚碳酸酯中引起的非晶化过程 .应用径迹饱和模型假设 (损伤过程只发生在面积为σ的柱形径迹内 ) ,分别给出了Fe,Xe和U离子在不同电子能损下辐照聚碳酸酯时的平均非晶化径迹半径 .用热峰模型对实验结果进行了检验 ,结果表明Szenes的热峰模型较好地描述了离子在聚合物中的潜径迹行为 .

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本文描述了荷能重离子束 (1 4氮 1 + 和 1 4氮 7+ )在作物改良上的应用。为了探索离子束轰击小麦种子不同部位(例如 :种皮 ,种胚和整粒种籽 ,包括胚乳、胚和种皮 )后的不同反应 ,采用了不同能量的氮离子。轰击不同部位是通过改变离子能量来实现的。在这个研究中 ,我们选择了三种能量以达到轰击不同部位的目的 ,它们是超低能区的110 ke V,低能区的 15 .7Me V/ u和中能区的 72 Me V/ u。根据 TRIM91程序计算 ,它们在种子内的射程依次为0 .4 4 μm,0 .6 1m m和 9.6 mm。所以 ,110 ke V的离子不能贯穿种皮 ,因为它的厚度 72 μm,只能极浅层注入种皮而不能触及胚细胞 (称这种情况为轰击部位 1) ,15 .7Me V/ u的离子能够贯穿种皮并注入胚内 (厚度约 1mm) ,但不能进入胚乳 (称这为轰击部位 2 ) ,72 Me V/ u的离子能从种子的胚部到顶部贯穿整个麦粒 (麦粒长约 7mm) (称这为轰击部位 3)。上述三种能量的氮离子辐照了三个品种 (定西 2 4、88- 12、82 - 5 79)的春小麦种子。而后进行了室内实验和大田培育 ,得到了 5 0 %出苗率时的剂量 D50 ,统计了上述三个轰击部位下根尖细胞中的微核率及染色体畸变率 ,大田中产生了一些新的变异 ,例如增产 (达百分之几十 ) ,早熟 (五天左右 ) ,矮杆 (低约 2 0 cm) ,抗 (条锈 )病 ,

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The CR superconducting magnet is a dipole of the FAIR project of GSI in Germany. The quench of the strand is simulated using FEM software ANSYS. From the simulation, the quench propagation can be visualized. Programming with APDL, the value of propagation velocity of normal zone is calculated. Also the voltage increasing over time of the strand is computed and pictured. Furthermore, the Minimum Propagation Zone (MPZ) is studied. At last, the relation between the current and the propagation velocity of normal zone, and the influence of initial temperature on quench propagation are studied.

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应用Simpson和Shannon多样性指数及其相应的均匀度,对阔叶红松林及其次生白桦林的高等植物物种多样性进行了对比研究.结果表明,对于木本植物,次生白桦林物种多样性高于阔叶红松林;而对于草本植物,情况正好相反.但在次生白桦林中,无论木本植物还是草本植物,占优势的物种都是一些常见种,而许多阔叶红松林中的珍稀或特有物种在次生白桦林中数量很少,有些甚至完全消失.对群落各种群多度分布的检验表明,2种森林类型中,无论木本植物还是草本植物,各种群的多度分布都遵从对数级数分布.

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森林温度特征的研究是揭示森林生态系统功能、评估森林环境效益的基础。对2004年长白山阔叶红松林林内外空气温度和土壤温度进行了观测,结果表明:林内外空气温度、土壤温度明显不同。白天林内气温一般低于林外,夜间高于林外。昼间气温差夏季较大,7月平均差值为1·6℃;夜间气温差冬季较大,1月平均差值为1·5℃。空气温度和土壤温度的日振幅总是林内小于林外,7月空气温度日振幅林内比林外低1·7℃,4月土壤温度日振幅林内比林外低5·8℃。

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使用多尺度分析方法分析了长白山阔叶红松林林冠大气界面湍流交换的主导时间尺度特征及相应的作用系数特征及大气层结和林冠结构的影响。发现:水平风速的DTS大于垂直风速的相应值,垂直风速的DCR大于水平风速的DCR。大气层结影响是非线性的。不稳定层结条件下的DTS和DCR大于稳定层结下。在强稳定层结和强不稳定层结条件下,DTS和DCR主要取决于来流特征。生长季节各高度DTS的变化规律较为复杂且不一致,非生长季节各高度具有一致的变化。不稳定层结条件下的生长季节水平风速DTS大于非生长季节;林冠结构可以显著影响主导时间尺度和主导过程贡献率。图2表1参15。