Cellular and Molecular Tissue Response to Triple Antibiotic Intracanal Dressing

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Development of a Methodology and Evaluation of Pesticides Against Aceria litchii and Its Predator Phytoseius intermedius (Acari: Eriophyidae, Phytoseiidae)

The litchi erineum mite, Aceria litchii (Keifer), is the major pest of litchi, Litchi chinensis Sonnerat (Sapindaceae). This study evaluated the effect of 11 pesticides on the survival of A. litchii as well as on the survival and reproduction of Phytoseius intermedius Evans& McFarlane, the predator most found in association with it in Brazil. The containment of A. litchii in small petri dishes whose bottoms were covered with a solidified paste made of a mixture of gypsum and activated charcoal (9: 1 in volume), kept humid, was shown to be adequate for this type of study. For the evaluation of the effect of pesticides on A. litchii, mites leaving the erinia from the pieces of litchi leaves (removed from the plants approximate to 24 h earlier) were sprayed under a Potter tower and immediately transferred to the 2.5-cm-diameter petri dishes. After 2, 12, 24, and 48 h of the application, the dishes were examined to evaluate the mite survival. The four pesticides causing the highest levels A. litchii mortality, as well as azadirachtin, were tested for the effect on P. intermedius. For this test, experimental units consisted of discs of uninfested litchi leaves also sprayed under a Potter tower before introducing the predators. Survival and oviposition of the predator were evaluated every 24 h for 5 consecutive days; viability of the eggs laid was also evaluated. Highest mortality of A. litchii occurred with the application of fenpyroximate, sulfur, abamectin, and hexythiazox. Azadirachtin was considered moderately harmful to the predator P. intermedius, whereas other pesticides were classified as harmful. Despite the low efficiency of azadirachtin in the control of the pest, its relative selectivity to P. intermedius would encourage the evaluation on field condition, especially for use in organic production systems.

Glutathione-s-transferase modified electrodes for detecting anticancer drugs

With the fast growth of cancer research, new analytical methods are needed to measure anticancer drugs. This is usually accomplished by using sophisticated analytical instruments. Biosensors are attractive candidates for measuring anticancer drugs, but currently few biosensors can achieve this goal. In particular, it is challenging to have a general method to monitor various types of anticancer drugs with different structures. In this work, a biosensor was developed to detect anticancer drugs by modifying carbon paste electrodes with glutathione-s-transferase (GST) enzymes. GST is widely studied in the metabolism of xenobiotics and is a major contributing factor in resistance to anticancer drugs. The measurement of anticancer drugs is based on competition between 1-chloro-2,4-dinitrobenzene (CDNB) and the drugs for the GST enzyme in the electrochemical potential at 0.1 V vs. Ag/AgCl by square wave voltammetry (SWV) or using a colorimetric method. The sensor shows a detection limit of 8.8 mu M cisplatin and exhibits relatively long life time in daily measurements. (C) 2014 Elsevier B.V. All rights reserved.

A cubic silsesquioxane modified with purpald (r) : preparation, characterization and a voltammetric application for determination of sulfite

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A novel hybrid composite formed by reaction of 4,5-diphenyl-2-imidazolethiol with hexacyanoferrate (iii) and subsequent reaction with copper (ii): preparation, characterization and a voltammetric application for determination of l-glutatione

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A Silsesquioxane Organically Modified with 4-Amino-5-(4-pyridyl)-4H-1,2,4-triazole-3-thiol: Thermal Behavior and Its Electrochemical Detection of Sulfhydryl Compounds

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

MTA e hidróxido de cálcio como materiais obturadores de canal radicular em reimplante tardio de dente de macaco

O tratamento endodôntico é uma etapa importante do protocolo de atendimento do reimplante dentário e tem uma relação direta com o aparecimento das reabsorções radiculares, maior causa de perda dos dentes reimplantados. O objetivo do trabalho foi analisar o processo de reparo no reimplante tardio de dente de macaco, utilizando o hidróxido de cálcio (Ca(OH)2) e o MTA como materiais obturadores de canal. Cinco macacos Cebus apella adultos tiveram seus incisivos laterais superiores e inferiores, direito e esquerdo, extraídos e deixados em meio ambiente por 60 minutos. Decorrido esse período, foi realizado o preparo biomecânico dos canais e os dentes foram divididos em dois grupos experimentais: grupo I - canal preenchido com pasta de Ca(OH)2 e grupo II - canal preenchido com MTA (Angelus®). Após o selamento da abertura coronária com ionômero de vidro, o ligamento periodontal foi removido e os dentes imersos em solução de fluoreto de sódio 2%, pH 5,5, por 10 minutos. Em seguida, os alvéolos foram irrigados com soro fisiológico e os dentes reimplantados e contidos por 30 dias com fio de aço e resina composta. A eutanásia dos animais foi realizada 60 dias após o reimplante. Os espécimes de ambos os grupos apresentaram reabsorção por substituição e pontos de anquilose ao longo dos três terços radiculares e ausência de reabsorção inflamatória. Não houve diferença significante entre os dois grupos com relação à reabsorção por substituição, porém a quantidade de anquilose foi significativamente maior no grupo do Ca(OH)2. Baseado nesses resultados conclui-se que o MTA pode ser uma opção clínica viável para a obturação de dentes tardiamente reimplantados que necessitam de um longo período de curativo com hidróxido de cálcio.

DEVELOPMENT OF A BIOMIMETIC SENSOR MODIFIED WITH HEMIN AND GRAPHENE OXIDE FOR MONITORING OF CARBOFURAN IN FOOD

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determination of carbofuran and diuron in FIA system using electrochemical sensor modified with organometallic complexes and graphene oxide

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Electroanalytical determination of bumetanide employing a biomimetic sensor for detection of doping in sports

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Controle da população de morcegos hematófagos na região de Andradina, São Paulo

Pós-graduação em Ciência Animal - FMVA

A new biomimetic sensor based on molecularly imprinted polymers for highly sensitive and selective determination of hexazinone herbicide

A new selective sensor based on molecularly imprinted polymers (MIPs) was developed for the determination of hexazinone (HXZ) in environmental samples. MIPs were synthesized using a non-covalent approach, and selection of the monomers employed in the polymerization reaction was carried out by molecular modeling. Three functional monomers with high (2-vinylpyridine (MP17)) and intermediate (methacrylic acid (MP12) and acrylamide (MP5)) energies of binding to the template (HXZ) were selected for preparation of the MIPs, in order to conduct comparative studies and validate the theoretical data. For sensor construction, carbon pastes were modified with each MIP or NIP (non-imprinted polymer), and HXZ determination was performed using differential pulse adsorptive cathodic stripping voltammetry (DPAdCSV). All parameters affecting the sensor response were optimized. In HCl at pH 2.5, the sensor prepared with MP17 (5% w/w in the paste) showed a dynamic linear range between 1.9 × 10−11 and 1.1 × 10−10 mol L−1, and a detection limit of 2.6 × 10−12 mol L−1, under the following conditions: accumulation time of 200 s at a potential of −0.5V, scan rate of 50 mVs−1, pulse amplitude of 60 mV, and pulse width of 50 ms. The sensor was selective in the presence of other similar compounds, and was successfully applied to the analysis of HXZ in river water samples.

Voltammetric behaviour of a modified electrode with azide copper octa(3-aminopropyl)octasilsesquioxane composite in the oxidation of ascorbic acid

A novel nanostructured composite, azide copper octa (3-aminopropyl)octasilsesquioxane (ASCA) was incorporated into a graphite paste electrode and the electrochemical studies were conducted with cyclic voltammetry. The cyclic voltammogram of the modified graphite paste electrode with ASCA (GPE-ASCA), showed one redox couple with formal potential (E  ) = 0.30 V and an irreversible process at 1.1 V (vs Ag/AgCl; NaCl 1.0 mol L-1 ; v = 20 mV s-1 ). The redox couple with (E  ) = 0.30V presents an electrocatalytic response for determination of ascorbic acid. The modified electrode gives a linear range from 1.010-4 – 1.010-3 mol L-1 (r = 0.998) for the determination of ascorbic acid with detection limit of 6.910-5 mol L-1 and standard deviation of 2.3% for n = 3 . The amperometric sensitivity was 122.1 mA/mol L-1 for ascorbic acid. The application this electrode was tested and ascorbic acid in three commercial pharmaceutical product (Cebion, Cewin and Redoxon) have been determined.

Avaliação histológica de estratégias de engenharia tecidual para a terapia endodôntica

Pós-graduação em Ciência Odontólogica - FOA

Espectroscopia de impedância eletroquímica da biodissolução da calcopirita na presença de íons ferrosos

Pós-graduação em Química - IQ