A novel hybrid composite formed by reaction of 4,5-diphenyl-2-imidazolethiol with hexacyanoferrate (iii) and subsequent reaction with copper (ii): preparation, characterization and a voltammetric application for determination of l-glutatione

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Processo FAPESP: 12/05438-1

Processo FAPESP: 12/11306-0

A novel Hybrid composite formed by interaction of 4,5-Diphenyl-2-Imidazolethiol and copper hexacyanoferrate (III) (CuHDIT) was characterized by Fourier transform infrared spectra (FTIR), x-ray diffraction (XRD), scanning electron and energy dispersive (SEM). CuHDIT was incorporated into a graphite paste electrode and the electrochemical studies were conducted with cyclic voltammetry. The cyclic voltammogram of the modified graphite paste electrode with CuHDIT, showed two redox couple with formal potential E-theta'=0.24 and 0.74 V (vs Ag/AgCl(sat.), KCl, 1.0 mol L-1; v = 20 mV s(-1)), attributed to the Cu+/Cu2+ and Fe2+(CN) 6/Fe3+(CN)(6) redox processes respectively. The redox second redox couple (0.74V) presents an electrocatalytic response for determination of L-Glutatione. The modified electrode showed a linear response from 5.0x10(-5) to 9.0x10(-4) mol L-1. The method showed a detection limit of 5.33x10(-5) mol L-1 with a relative standard deviation of +/- 2% (n = 3) and amperometric sensitivity of 12.52 mA mol L-1.