999 resultados para imuno-fluorescência direta


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Pós-graduação em Ciência e Tecnologia Animal - FEIS

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The crop demand by nitrogen varies from one crop to other as well as the amount and application time, and this nutrient can be lost by leaching, volatilization and erosion. The objective of this study was to evaluate doses, sources and times of nitrogen application in rice of high lands cropped in no till system. The work was conducted in Selviria, State of Mato Grosso do Sul, Brazil, in a soil originally under Cerrado vegetation. A randomized blocks design, with the treatments disposed in a factorial scheme 5x3x2 was used. The treatments were constituted by five nitrogen doses (0, 50, 100, 150 and 200 kg ha(-1)), three nitrogen sources (urea; ammonium sulfonitrate plus nitrification inhitor and ammonium sulfate) and two application times (at sowing or at flower bud differentiation), with four repetitions. The sources of nitrogen, as well as the application times had similar effects for most of evaluations. The N doses influenced linearly or with quadratic adjustment the plant height, N leaf content and grains yield, in the two growing seasons. The chlorophyll content and the number of ears m(-2) were affected only, in the first year. The highest yield in the growing season of 2007/08 was obtained with the dose of 149 kg ha(-1) of N. In 2008/09 growing season the increase of N doses provided increment in the grains rice yield, until the maximum dose evaluated (200 kg ha(-1) of N).

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O presente trabalho de conclusão de curso reporta os resultados obtidos durante o estágio de Iniciação Científica realizado no Núcleo de Biossíntese, Bioensaios e Ecofisiologia de Produtos Naturais do Departamento de Química Orgânica do Instituto de Química da UNESP-Araraquara. Foram realizados ensaios de triagem de substâncias naturais, semissintéticas e sintéticas com atividade inibitória sobre a protease aspártica pepsina e a protease serínica subtilisina. As amostras foram obtidas de extratos vegetais e de fungos endofíticos e foram testadas tanto substâncias puras naturais como diterpenos clerodânicos, cromenos, peptídeos e amidas bem como derivados sintéticos do ácido caféico, ferúlico, alcalóides piridínicos, entre outros resultantes das pesquisas realizadas por pesquisadores do NuBBE. Resultados mostraram que os ensaios de inibição da pepsina e da subtilisina apresentaram seletividade para os diferentes tipos de substâncias testadas. Ainda mais, foi possível observar diferenças nos resultados obtidos com os enantiômeros dos cromanos e dos cromenos. As substâncias que apresentaram maiores porcentagens de inibição foram os cromenos, os derivados do ácido cafeico, do ácido ferúlico e do ácido benzóico, as amidas, bem como os diterpenos clerodânicos. Alguns destes resultados foram publicados em revistas indexadas (Flausino et al., 2009; López et al., 2010; Oliveira et al., 2011) e outros estão sendo preparados para publicação

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In chemical industry, petrochemical and oil refinery may be found situations that working pressures and very high temperatures, and alloys that comprise such equipment must have specific characteristics such. As a way to ensure that the materials are as designed, companies have some specific techniques, and one of them is X-ray fluorescence spectrometry, which allows its application in devices small and lightweight, which ensures its portability in field use. This technique however, has some shortcomings such as the impossibility of detecting carbon and other elements of low atomic number which can generate false identifications of elements that are not in the league or non-detection of existing elements. Therefore, this study investigated the reliability of this method using the apparatus Niton XL3t 800 and may conclude that their results are reliable and can be used to assist in ensuring the operational integrity of the production units of these companies

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The aims of this work are to analyze the direct solar radiation pressure torque (TPRS) in the rotational motion of spin-stabilized artificial satellites, to numerically implement these solutions and to compare the results with real data of the Brazilian Satellite Data Collection – SCD1 and SCD2, supplied by INPE. The mathematical model for this torque is determined for a cylindrical satellite, and the components of this torque are determined in a fixed system in the satellite. An analytical solution for the spin motion equations is proposed, in which TPRSD does not affect the spin velocity of the satellite. Two approaches are adopted in the numerical implementation of the developed theory: the first one considers the proposed theory and the second introduces a variation in the spin velocity based on its real variation. The results obtained indicate that the solar radiation pressure torque has little influence in the right ascension and declination axis of rotation due to the small dimension of the satellite and altitude in which it is found. To better validate the application of the presented theory, the angular deviation of the spin axis and solar aspect angle were also analyzed. The comparison of the results of the approaches conducted with real data show good precision in the theory, which can be applied in the prediction of the rotational motion of the spin-stabilized artificial satellites, when others external torques are considered besides the direct solar radiation pressure torque

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O ipê-branco é uma espécie de difícil propagação por sementes devido à sua curta longevidade. A cultura de tecidos tem possibilitado a melhor propagação de diversas espécies, tendo como base o crescimento de células, tecidos e órgãos em condições assépticas, possibilitando a rápida multiplicação da planta selecionada, obtendo mudas livres de patógenos. A primeira etapa da cultura de tecidos está baseada na desinfestação das sementes para obter as mudas in vitro, possibilitando as posteriores etapas do processo. Foram testadas diversas concentrações de álcool etílico e hipoclorito de sódio em busca da desinfestação mais efetiva. O melhor processo para a desinfestação das sementes do ipê branco está nos tratamentos de germinação em apenas ágar com a retirada das alas da semente após a sua lavagem, com passagem por 30 segundos em álcool etílico e 5 minutos em hipoclorito de sódio, com posterior transferência da plântula para meio MS suplementado com 0,7% de ágar e 3% de sacarose. Sobre as plantas germinadas in vitro foram aplicados tratamentos com hormônios vegetais em busca das melhores concentrações para a obtenção de novas mudas. Os explantes caulinares tratados com BAP a 4,0 mg L-1 apresentaram 93,3% de brotações bem desenvolvidas, enquanto em meio WPM a melhor concentração para brotação com BAP é a de 4,0 mg L-1, não promovendo no entanto um tratamento tão efetivo como em meio MS. Para cinetina, todos os tratamentos exibiram formação de calo e/ou ausência de brotações em meio MS, e baixa porcentagem de brotação em meio WPM. Os tratamentos realizados com AIB e ANA não tiveram rizogênese efetiva em meio MS, apresentando formação de raízes pouco desenvolvidas em apenas poucos explantes no tratamento de 4,0 mg L-1 de ANA em meio WPM

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The behavior and experience of pain are characterized by alertness and anxiety, which disappear soon after the healing process begins. Based on this area, the present study aimed to quantify the expression of c-fos in segments of the brain of rats after surgical stimulation in one rear hind paw (analgesia), using anesthesia with sodium thiopental and practices of acupuncture pre and post operative. The animals were stimulated with intraperitoneal injections of solution (NaCl) 0.2% (2ml), and divided into three groups, control, preoperative and postoperative. Each treatment was divided in manual acupuncture (AM) and electroacupuncture (EA). The animals were randomly distributed in each group. The c-fos expression was quantitated using immunohistochemistry for all situations. The results showed a great efficiency of all treatment compared to the control group (p <0.001), thus reenforcing data in the literature on the potential of acupuncture analgesia. There were no statistical differences among the different treatments, although there was a trend of EA being more efficient than AM

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Increasingly competitive markets have driven the search for companies in many different ways to win and keep customers. The service level is basically the performance of companies in fulfilling the orders made, or how companies demonstrate to their clients efforts in their behalf. This work aims to solve the difficulties faced by a multinational company present in Brazil, in the distribution of its products in the category Ice Cream in order to improve the service level of their customers. Review the logistics network and concepts related to the distribution system of products is one of several ways to achieve this goal, as well as the use of IT and tools to assist in planning and programming of the physical distribution of products. In this study we used the concept of direct distribution system called Transit Point (TP). The TP provides at the same time, a strategy of rapid response, flexibility, low transportation costs and no inventory. A router - software capable of simulating the actual conditions experienced in the daily distribution - was used to assist in calculations. Results showed reductions of up to 47.5% in transportation costs and better conditions were provided in the distribution of products, positively impacting on service levels and in the maintenance of products quality, with a reduction of 1.6% of the total costs involve

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Pós-graduação em Agronomia (Produção Vegetal) - FCAV

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After a long period of military dictatorship, the Brazilian people, following the redemocratization in the late 1980s, experimented the possibility of electing their political representatives. This experience of democracy and citizenship included in the electoral process, now, appear as one of the main challenges to make the Brazilian public schools more democratic and participative, through the election of school managers. The purpose of this paper is to discuss the historical aspects of the process of electing representatives in Brazil and the possibilities of direct elections for school managers. At first recovering the struggle of the Brazilian society in search of an effective political participation and, subsequently, pointing out the limits and possibilities of the introduction of direct elections for school managers as a mechanism to consolidate a more democratic school.

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Pós-graduação em Agronomia (Agricultura) - FCA

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV