Novel Antimicrobial Peptides Bacterial Cellulose Obtained by Symbioses Culture Between Polyhexanide Biguanide (PHMB) and Green Tea

Bacterial cellulose is a highly hydrated pellicle made up of a random assembly of ribbon shaped fibers less than 5 nm wide. The unique properties provided by the nanometric structure have led to a number of diagnostic biological probes, display devices due to their unique size-dependent medical applications. Bacterial cellulose matrix extracellular is a novel biotechnology and unique medicine indicated for ultimate chronic wound treatment management, drug delivery, tissue engineering, skin cancer and offers an actual and effective solution to a serious medical and social problem and to promote rapid healing in lesions caused by Diabetic burns, ulcers of the lower limbs or any other circumstance in which there's epidermal or dermal loss. In this work, it is reported novel antimicrobial peptides (AMPs) bacterial cellulose/polyhexanide biguanide (PHMB) which are produced by symbioses culture between polyhexanide biguanide and green tea culture medium resulting in the pure 3-D structure consisting of an ultra-fine network of novel biocellulose/PHMB nanofibres matrix (2-8 nm), highly hydrated (99% in weight), and with higher molecular weight, full biocompatibility.

Utilization of Minimum Quantity Lubrication (MQL) with Water in CBN Grinding of Steel

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Does fire induce flowering in Brazilian subtropical grasslands?

QuestionsWe aimed to analyse the effect of fire on flowering in subtropical grasslands, by addressing the following questions: will fire history affect flowering? If yes, do fire feedbacks influence flowering or is it just the removal of above-ground biomass? Are there differences in burned and mowed plots?LocationSubtropical grasslands in Southern Brazil (30 degrees 03S, 51 degrees 07W).MethodsWe established plots in areas with different fire histories: 30d (30 plots: five replicates), 1yr (14 replicates), 3yr (30 plots: five replicates) since the last fire, in experimentally burned and mowed plots (14 replicates each). We counted the number of flowering species, as well as the number of flowering stalks.ResultsGraminoid species flowered in highest numbers 1yr after fire, whilst forbs had more species flowering just after fire, indicating different reproductive strategies in post-fire environments. Mowing was not as efficient as fire in stimulating flowering. Finally, the different functional groups showed different flowering responses to time since last fire and to the different types of management.ConclusionsOur results show fire stimulated flowering. Although mowing can be a good alternative for maintaining plant diversity, our study showed that this practice is not as efficient as fire in stimulating flowering. However, fire season should be noted as a limiting factor to the recovery of C-3 grasses in these subtropical grasslands, and annual burns may be harmful to C-4 grasses, since they delay their flowering to the next post-fire growing season.

Development of organic-inorganic polymeric film formers for controlled drug release and wound care

Film forming polymeric systems represents a new and unexplored technology of systems forskin or wounds protection and for controlled drug release. The aim of this work was to study the use of polymeric organic-inorganic ureasil-polyether hybrids synthesized by the sol-gel process as film forming system containing silver sulfadiazine as model drug. The film formationtime can be controlled by changing the precursor/catalyst ratio used during the step of hydrolysis and condensations. The results showed that the precursor/catalyst proportion influences both the visual characteristics and time required to form the film. The precursor/catalyst ratio equal to 20.8 m/v was considered ideal due to promote the homogeneous and transparent film formation in less than 5 minutes. The release profile of sulfadiazine is dependent on the characteristics of the matrixes: matrix more hydrophobic as ureasil-POP provided a slowed released mainly due to the low swelling of the matrix. The more hydrophilic ureasil-POE matrix presents a large capacity to swell and favors the faster release of the drug. The set of results showed the possibility of future use of these systems for treating wounds caused by burns.

Reflexão sobre prevenção de acidentes à criança no âmbito doméstico

The aim was to reflect what action to prevent accidents used by caregivers / participants in the home. This is a prospecive cross-sectional descriptive study conducted at the Pediatric Inpatient Unit, Hospital of the Medical School of Botucatu – Unesp, during the months from May to August 2010.A questionnaire was used in the form of check list (Annex I) on measures to prevent accidents. The participant chose two alternatives taking into consideration the order of priority in the prevention of accidents. This study shows that for the age group from 29 days to 2 years, the companions chose these preventive measures for falls, burns, poisoning and drowning, “do not put near the windows mobile” (27.8%), “no access the match, lighter and appliances (22.2%), leave toxic substances out of reach and sight of children (41.7%) and empty buckets, tubs and pools after use (27.8% ). Regarding the age group 2-6 years, the measures for these types of accidents were not put near the windows mobile (26.5%), do not have access to matches, lighters and household appliances (20.9% ), leave toxic products out of reach and sight of children (27.9%) and maintain tanks, tanks and wells or struck with some protection (20.2%). For ages 6 to 10 years, the measures chosen to more such accidents were dry liquid spilled on the ground (23.5%), do not have access to matches, lighters and household appliances (22.5% ), leave toxic products out of reach and sight of children (31%) and keep tanks, tanks and wells or struck with some protection (23.5%). One fact that attracted our attention was that the preventive measures most frequently used by caregivers, regardless of age, show results that differ in few alternatives. It is essential to the understanding of the escorts / participants on the importance of injury prevention. Even today is predominantly defined as accident, injury must be addressed through measures

Avaliação da produtividade em áreas de campo sujo com diferentes históricos de fogo

Fire is a common event in different ecosystems and can both be caused by humans or have natural sources.. In many of these ecosystems, natural fires are an important factor that determines the vegetation. The reduction of tree cover by fire for example, resulted in the evolution of several species-rich ecosystems, dominated by C4 grasses. However, the fire caused by human actions may have greater intensity and lead to negative responses of vegetation, since man changed the fire regime in many parts of the world, such as in the Cerrado. The passage of fire can benefit herbaceous and woody seedlings that cannot compete with the dominant grass layer. It removes the dead biomass and litter (major components of the fuel load), opening up spaces within the grass matrix that allow the establishment of other species. After some time without fire, an increase in shrub cover and decrease herbaceous layer can be observed. One of the major consequences of the absence of fire in savanna and grassland ecosystems is the accumulation of flammable dead biomass (mainly composed of graminoids), which will probably be the fuel load of the next burning thus, fires will be more intense and hotter. Moreover, very frequent burns lead to a reduction in the frequency and density of grasses. Therefore, this study aimed to assess the quantity and quality of biomass in areas with different fire history (fire exclusion for 2 and 7 years) in areas of campo sujo in central Cerrado. Plots (1x1m) were established in both areas and all aboveground biomass of each plot was cut at ground level and put in paper bags in the field. In the laboratory, the material was sorted into live and dead biomass. In addition, live biomass was separated into different functional groups (graminoids, forbs, Vellozia spp, palm and shrubs). The material was oven dried for two days at 80°C and subsequently weighed. In both areas, we found a dominance of graminoid and dead biomass. The area...

Recursos humanos e materiais na assistência de enfermagem a pacientes de uma unidade de tratamento de queimaduras

The burn is among the leading causes of morbidity and mortality in our society. It shows a great complexity and is hard to treat. Beyond the physical suffering, the burned patient is affected by psychological distress, requiring a high level of knowledge for assistance planning. The Professional Practice Law No. 7498 establishes the nurse as in charge of the client, as leader of the nursing team and as responsable for the management of physical and human resources. The nurse has autonomy to design the quantitative and qualitative picture of the nursing staff and should use the methodologies for their suitability to the real levels of assistance needed. Material resources represent 15 to 25% of total expenditures at health organizations. Therefore, to maintain the care level, nurses must determine the needs, considering the quantitative, qualitative and financial aspects. The study aimed the survey of the human and material resources necessary for nursing care to patients in a Burns Treatment Unit and identify its epidemiological profile and its nursing diagnoses. We collected the data from medical records of hospitalized between July and August, and the nursing diagnoses were classified through the Taxonomy II proposed by the North American Nursing Diagnosis Association (NANDA). The design of the picture of nurses followed the parameters of COFEN Resolution nº 293/2004. We apply the Fugulin's Patients Classification System to establish levels of the required care. The institution's Cost Center provided a spreadsheet with the purchased items, subsequently classified into ABC. Most hospitalized patients were men, aged between 20 and 50. There was a predominance of patiences with minor burned and the most common type of burn was due to fire. The average residence time was 28.71 days, and 88% of the patients were discharged... (Complete abstract click electronic access below)

Perfil epidemiológico dos pacientes internados na unidade de tratamento de queimaduras do Hospital Estadual de Bauru

Burns are frequent accidents, and they are among the major causes of morbidity and mortality in our society. In Brazil, statistical data on such lesions are relatively scarce, and they are concentrated in a few treatment centers. Data on such accidents allow for specific care to be timely and properly taken in order to provide patients with a better prognosis, in addition to enabling the development of prevention programs as well as fire-prevention safety laws. To characterize the epidemiological profile of users at the Burn Treatment Unit of Bauru State Hospital. Collection of epidemiological data through the e-pront system and from charts of burned inpatients from April 2005 to April 2010. During the studied period, 906 hospitalizations of burned patients occurred, with an annual average of 181.2 individuals. Their mean age was 28.4 years, with a predominance of males. The mean hospitalization period was of 27.6 days, with a median of 16 days and a maximum of 216 days; 14.7% developed to death, with the highest mortality rate in the first two weeks of hospitalization. Second-degree burns were the most frequent; 36.8% of the patients burned less than 10% of their body surface, and there was a gradual frequency reduction when a larger burned surface was taken into account; the greater the affected areas, the higher the number of deaths; 91% resulted from thermal agents, 6% from electric and 2% from chemical agents; scalding was the major cause among all agents, followed by alcohol associated with fire and direct flame. The major area affected areas were the upper limbs. The study shows the need for educational and preventive measures, particularly for the young adult population, by means of educational programs and prevention campaigns

Imagens de satélite na análise da relação entre queimadas, aerossóis atmosféricos e precipitação

The direct and indirect effects of atmospheric aerossols in the amazonic climate have been focus of many published. This work aims to analyze a possible relation among Burns, atmospheric aerossols and precipitation above South America. It uses the avaluable data from the satellites AQUA/TERRA and TRMM and the images furnished by NOAA and GOES. These analysis can provide some explanations about the effects of emission of aerosols by burns on the composition of atmosphere and cloud formation, mainly in Amazon. We use the Aerosol Optical Depth (AOD) from MODIS/TERRA-AQUA, burns index from CPTEC and rain rate from TRMM. The data we use were obtained from 2000 until 2012. The study is divided in two parts. In the first one, it was performed a quantitative analysis between number of burns and aerosols emission. It was identified a great variability in space and time of the AOD on South America. On the north, northeast and center-west, the AOD is significant during the winter period, with peak on August and September. The southeast is affected by aerosols from center-west due to the dynamical transport. In the second part, it was evaluated the relation between AOD and precipitation in a 13-year period. The statistical analysis shows up a negative correlation of 0.72 between August and October, on Legal Amazon. These result indicate an inverse relation between AOD and rain rate. The other months present not significant correlation. These results are in a good agreement with the literature, in which in-situ methods were applied or combined with satellites data. The increasing of aerosols concentration in the atmosphere are reinforced during drier years. It can affect the increasing process of water drops, decreasing the precipitation. We also verified higher values of AOD (0.25 - 0.3) during years with El Niño, than the climatologically average (~0.15 - 0.2), ... (Complete abstract click electronic access below)

Casearia sylvestris Swartz Extract Release Using Natural Rubber Latex Biomembranes as Carrier

The Natural Rubber Latex (NRL) from Hevea brasiliensis has shown promise in biomedical applications due to its low cost, easy handling, mechanical properties and biocompatibility, being used for bone regeneration and wound healing due to its natural stimulus to angiogenesis. The aim of this work was to incorporate Casearia sylvestris Sw. extract in NRL biomembranes and study its release behavior. The complex membrane-extract has as object of study a new approach of using C. sylvestris extract in the treatment of wounds, for possessing antiseptic activity, anti-inflammatory and analgesic properties. The C. sylvestris species (Salicaceae), popularly known as "guaçatonga", presents great distribution and is used in folk medicine as antiulcer, wound healing, anti-snake venom, properties which have been proven and related to clerodane diterpenes (casearins A-X). The release rate of C. sylvestris compounds from extract-membrane complex was monitored and analyzed using the method of optical spectroscopy (UV-VIS). The release varied with temperature ranging from 14 to 33 days, releasing more than 90%, with an interesting and promising biomedical application, such as wound healing and burns.

Conceptos actuales en la preparación de la cavidad apical para obturación retrógrada en cirugía perirradicular

The endodontic surgery is indicated in cases of failure and/or impossibility of conventional endodontic treatment. Among the existent modalities in the endodontic surgery, the retrograde filling consists on preparation of apical cavity and filling with a retrofilling material. The technical-scientific progress in Endodontics become possible the use of retrotips activated with ultrasonic for retrocavities preparation with appropriate extension and following the long axis of the root. The aim of the study was to present the current resources in the preparation of the retrograde cavity in endodontic surgery, detaching their advantages in comparison with the use of conventional preparation with burns. A review of the literature will be presented, considering the use of ultrasound and laser for retrograde preparation, seeking the current resources that provide better apical preparation, resulting in better prognostic of the endodontic surgery (AU)

Violence against children and adolescents: awareness and attitudes of Brazilian dentists

Violence against children and adolescents, ranging from negligence to sexual abuse, is an imperative public health problem. The ill treatments are considered as nonaccidental traumas, actions or omissions against children, who suffer physical or emotional violence. The main perpetrators are fathers, mothers, guardians, family, friends or the children‘s primary caretaker. It is very important to identify abuse cases, because this allows proper medical and legal intervention. In Brazil, the law n. 8.069, of July 13, 1990, known as the ECA -Statute for Children and Adolescents (SCA), stipulates the obligation to report suspected or confirmed cases of ill treatment against a child or adolescent to the Guardianship Council of the respective locality. The health professionals play a crucial role with regard to identifying cases of violence, collecting information, making early diagnosis of suspect cases and reporting such cases to the authorities. The dentists can contribute significantly, as most injuries occur in the orofacial region. Bite marks, burns, bruising, among others, are easily identified during a dental consultation. The aim of this work was to verify the awareness and attitudes of Brazilian dentists concerning violence against children and adolescents. This research was approved by the Research Ethics Committee of the Faculty of Dentistry of Araraquara – São Paulo State University (FOAr – UNESP). Sixty-three dentists answered an open and closed questionnaire concerning their formation, knowledge, experience and attitudes towards ill-treated children and adolescents. Among other results, thirty-nine dentists (61.9%) affirmed to have the means to identify illtreatment cases, 13 (20.6%) reported having some experience on this matter, but only 8 reported the cases to the Council of Guardianship, as determined by Brazilian law. Twenty percent of the reasons presented for not notifying the Council were fear of reprisal, and 60.0% were uncertain concerning the ethical and legal implications. Physical violence was the most reported form of identified violence (76.9%), followed by negligence (38.5%). Among the 13 professionals that had experience with violence cases, in 10 cases the perpetrators were identified – in 70.0% of the cases, the parents were the originators of such violence. It was concluded that further formation and orientation are necessary, in order to prepare dentists to act correctly when, during their professional activities, they encounter cases of violence against children and adolescents.

In vivo evaluation of the genetic toxicity of Rubus niveus Thunb. (Rosaceae) extract and initial screening of its potential chemoprevention against doxorubicin-induced DNA damage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Could histoplasma capsulatum be related to healthcare-associated infections?

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Induction of luteolysis in mares utilizing a micro-dose of prostaglandin F-2 alpha in the sacral lumbar space

An experiment was conducted to examine the luteolytic effectiveness of using low or micro doses of PGF2α when administered at the BAI HUI acupuncture point, which is frequently used to treat ovarian disturbances in veterinary acupuncture. The results indicate that PGF2α given at a very low micro dose of 0.5 mg (one tenth the conventional recommended dose) administered at the BAI HUI acupuncture point located at the sacral lumbar space is equally effective at inducing luteolysis in mid-luteal phase mares as conventional PGF2α i.m. treatment using a tenfold higher dose. Therefore, based on the results of the present study, we suggest that the BAI HUI sacrai lumbar route somehow provides an extremely efficient pathway for the drug to the ovarian level.