Impact of a neonatal parenteral nutrition on the hepatic activity of methionine adenosyltransferase, a limiting step for glutathione synthesis

Problématique : Le glutathion est une molécule clé de la défense antioxydante. Chez les enfants sous nutrition parentérale (NP), particulièrement les nouveau-nés, sa concentration tissulaire est anormalement basse. Puisque la capacité de synthèse de glutathion est adéquate, un déficit en cystéine, le substrat limitant, est soupçonnée. À cause de son instabilité en solution, la cystéine est peu présente en NP; la méthionine étant le précurseur endogène de cet acide aminé. L’activité de la méthionine adénosyltransférase (MAT), une enzyme essentielle à la transformation de la méthionine en cystéine, est facilement inhibée par l’oxydation. L’hypothèse : Le faible taux de glutathion chez les enfants sous NP est causé par l’inhibition de la MAT par les peroxydes contaminant ces solutions nutritives. Objectif: Mesurer l’impact d’une infusion de NP et de H2O2 sur l’activité hépatique de MAT en relation avec le niveau de glutathion. Méthode : Un cathéter est placé dans la jugulaire droite de cobayes de trois jours de vie. Quatre groupes sont comparés:1- Témoin (animaux aucune manipulation, sans cathéter) 2)-(animaux nourris normalement et le cathéter (noué)); 3) NP (animaux nourris exclusivement par voie intraveineuse (acides aminés + dextrose + lipides + vitamines + électrolytes), cette solution génère environ 400 µM de peroxyde. 4) H2O2 (animaux nourris normalement et recevant via le cathéter 400 µM de H2O2). Après quatre jours, le foie et le sang sont prélevés pour la détermination du glutathion, potentiel redox et l’activité de MAT, glutathion peroxydase et glutathion reductase. Résultats : L’activité de MAT est plus faible dans les groupes NP et H2O2. Le potentiel redox du foie et dans le sang est plus oxydé dans le groupe NP. Tandis que la concentration de GSSG du foie est plus élevée dans le groupe NP. Ainsi la concentration de GSH dans le sang et foie est plus faible dans les NP et H2O2 Discussion: La relation entre l’inhibition de MAT et le stress oxydant observée dans le groupe NP pourrait bien expliquer la perturbation du système glutathion observée chez les nouveau-nés prématurés.

Assessment of PEG on Polymeric Particles Surface, a Key Step in Drug Carrier Translation

Injectable drug nanocarriers have greatly benefited in their clinical development from the addition of a superficial hydrophilic corona to improve their cargo pharmacokinetics. The most studied and used polymer for this purpose is poly(ethylene glycol), PEG. However, in spite of its wide use for over two decades now, there is no general consensus on the optimum PEG chain coverage-density and size required to escape from the mononuclear phagocyte system and to extend the circulation time. Moreover, cellular uptake and active targeting may have conflicting requirements in terms of surface properties of the nanocarriers which complicates even more the optimization process. These persistent issues can be largely attributed to the lack of straightforward characterization techniques to assess the coverage-density, the conformation or the thickness of a PEG layer grafted or adsorbed on a particulate drug carrier and is certainly one of the main reasons why so few clinical applications involving PEG coated particle-based drug delivery systems are under clinical trial so far. The objective of this review is to provide the reader with a brief description of the most relevant techniques used to assess qualitatively or quantitatively PEG chain coverage-density, conformation and layer thickness on polymeric nanoparticles. Emphasis has been made on polymeric particle (solid core) either made of copolymers containing PEG chains or modified after particle formation. Advantages and limitations of each technique are presented as well as methods to calculate PEG coverage-density and to investigate PEG chains conformation on the NP surface.

Design of a microstip fed step slot antenna for UWB communication

The design and performance of a stepped slot printed monopole antenna in the ultrawideband is presented in this article. Multiple resonances generated by the stepped slot geometry are matched in the ultrawideband using a modified microstrip feed. The impedance bandwidth (SWR < 2) of the antenna is from 3 to 11 GHz. Radiation patterns are stable and omnidirectional with appreciable gain throughout the band. Performance of the antenna is also analyzed in the time domain, which reveals good pulse handling capabilities. Compact geometry of the antenna allows easy commercial deployment.

Giant step bunching from self-organized coalescence of SrRuO3 islands

Step bunching develops in the epitaxy of SrRuO3 on vicinal SrTiO3(001) substrates. We have investigated the formation mechanisms and we show here that step bunching forms by lateral coalescence of wedgelike three-dimensional islands that are nucleated at substrate steps. After coalescence, wedgelike islands become wider and straighter with growth, forming a self-organized network of parallel step bunches with altitudes exceeding 30 unit cells, separated by atomically flat terraces. The formation mechanism of step bunching in SrRuO3, from nucleated islands, radically differs from one-dimensional models used to describe bunching in semiconducting materials. These results illustrate that growth phenomena of complex oxides can be dramatically different to those in semiconducting or metallic systems.

Bayesian Analysis of Simple Step-stress Model under Weibull Lifetimes

Department of Statistics, Cochin University of Science & Technology, Part of this work has been supported by grants from DST and CSIR, Government of India. 2Department of Mathematics and Statistics, IIT Kanpur

Many-body theory of laser-induced ultrafast demagnetization and angular momentum transfer in ferromagnetic transition metals

An electronic theory is developed, which describes the ultrafast demagnetization in itinerant ferromagnets following the absorption of a femtosecond laser pulse. The present work intends to elucidate the microscopic physics of this ultrafast phenomenon by identifying its fundamental mechanisms. In particular, it aims to reveal the nature of the involved spin excitations and angular-momentum transfer between spin and lattice, which are still subjects of intensive debate. In the first preliminary part of the thesis the initial stage of the laser-induced demagnetization process is considered. In this stage the electronic system is highly excited by spin-conserving elementary excitations involved in the laser-pulse absorption, while the spin or magnon degrees of freedom remain very weakly excited. The role of electron-hole excitations on the stability of the magnetic order of one- and two-dimensional 3d transition metals (TMs) is investigated by using ab initio density-functional theory. The results show that the local magnetic moments are remarkably stable even at very high levels of local energy density and, therefore, indicate that these moments preserve their identity throughout the entire demagnetization process. In the second main part of the thesis a many-body theory is proposed, which takes into account these local magnetic moments and the local character of the involved spin excitations such as spin fluctuations from the very beginning. In this approach the relevant valence 3d and 4p electrons are described in terms of a multiband model Hamiltonian which includes Coulomb interactions, interatomic hybridizations, spin-orbit interactions, as well as the coupling to the time-dependent laser field on the same footing. An exact numerical time evolution is performed for small ferromagnetic TM clusters. The dynamical simulations show that after ultra-short laser pulse absorption the magnetization of these clusters decreases on a time scale of hundred femtoseconds. In particular, the results reproduce the experimentally observed laser-induced demagnetization in ferromagnets and demonstrate that this effect can be explained in terms of the following purely electronic non-adiabatic mechanism: First, on a time scale of 10–100 fs after laser excitation the spin-orbit coupling yields local angular-momentum transfer between the spins and the electron orbits, while subsequently the orbital angular momentum is very rapidly quenched in the lattice on the time scale of one femtosecond due to interatomic electron hoppings. In combination, these two processes result in a demagnetization within hundred or a few hundred femtoseconds after laser-pulse absorption.

Numerical Simulation of Electroosmotic Flow with Step Change in Zeta Potential

Electroosmotic flow is a convenient mechanism for transporting polar fluid in a microfluidic device. The flow is generated through the application of an external electric field that acts on the free charges that exists in a thin Debye layer at the channel walls. The charge on the wall is due to the chemistry of the solid-fluid interface, and it can vary along the channel, e.g. due to modification of the wall. This investigation focuses on the simulation of the electroosmotic flow (EOF) profile in a cylindrical microchannel with step change in zeta potential. The modified Navier-Stoke equation governing the velocity field and a non-linear two-dimensional Poisson-Boltzmann equation governing the electrical double-layer (EDL) field distribution are solved numerically using finite control-volume method. Continuities of flow rate and electric current are enforced resulting in a non-uniform electrical field and pressure gradient distribution along the channel. The resulting parabolic velocity distribution at the junction of the step change in zeta potential, which is more typical of a pressure-driven velocity flow profile, is obtained.

Enhancing fault management performance of two-step QoS routing algorithms in GMPLS networks

In this paper a novel methodology aimed at minimizing the probability of network failure and the failure impact (in terms of QoS degradation) while optimizing the resource consumption is introduced. A detailed study of MPLS recovery techniques and their GMPLS extensions are also presented. In this scenario, some features for reducing the failure impact and offering minimum failure probabilities at the same time are also analyzed. Novel two-step routing algorithms using this methodology are proposed. Results show that these methods offer high protection levels with optimal resource consumption

Academics' online presence guidelines: A four step guide to taking control of your visibility

OpenUCT published Academics' online presence guidelines: A four step guide to taking control of your visibility in 2012.

Step by Step. 'Paso a paso'.

Se desarrolla un proyecto de innovación educativa que trata de promoverle uso del ingles en el aula de Infantil y de Primaria, de forma natural, fluida y lo más práctica posible. Para ello se desarrollan actividades que estimulen la expresión y la comprensión oral y los procedimientos que ayuden a comunicarse en una segunda lengua. El proyecto educativo se caracteriza por tener como meta una educación bilingüe, para ello se implica parte del profesorado en una realidad social y cultural existente, y a los padres para que el proyecto tenga continuidad en el ámbito familiar. Se han utilizado juegos didácticos, puzzles, fichas de aprendizaje, murales y dibujos ilustrativos, fotografías y cuentos en ingles. En general se ha alcanzado el objetivo de introducir el bilingüismo al ámbito escolar. La participación del profesorado es total, pero donde mejores resultados se han obtenido ha sido en Educación Infantil. La evaluación ha sido continua en todas las áreas impartidas, pero sobretodo en la de lengua inglesa. El proyecto tanto en su confección, como en su desarrollo e implantación ha favorecido el trabajo en equipo, y el amor de los alumnos y alumnas a la lengua inglesa.

Step into--the stone age.

Retrocedamos dos millones de años, a la Edad de Piedra y descubriremos como eran los ancestros de los modernos humanos. Dónde vivían los homínidos, como se agrupaban para cazar caballos, bisontes, renos, mamuts peludos que vagaban en grandes manadas por la tundra y las praderas. Se alimentaban de semillas, bayas, frutos secos, raíces, y ya tenían animales domesticados. Nos maravilla las buenas herramientas y armas que fabricaban con distintos materiales y que les ayudaba en su supervivencia. Una serie de actividades prácticas ayuda a los niños a recrear este mundo de la Edad de Piedra.

Step by wicked step.

En una tormentosa noche, unos estudiantes descubren en una abandonada habitación secreta, un misteriosos diario. Éste cuenta la historia de un chico llamado Richard Clayton Harwick que toma la decisión de huir de su casa por la aversión que le tiene su padrastro. Los muchachos ven reflejada en esta historia, su situación personal y familiar, sienten que todos ellos se encuentran con los mismos problemas,temores y preocupaciones de tener unos padres divorciados o que han vuelto a casarse.

Amazing science experiments : 150 easy projects using everyday materials, demostrated step by step in 1300 photographs.

Colección de ciento cincuenta experimentos que explican hechos y procesos científicos, y demuestran cómo funcionan las máquinas. Cada experimento se introduce, describe y explica, y esta información no sólo permite al usuario trabajar con confianza, sino que también proporciona una gran cantidad de conocimiento científico adicional para los niños de ocho a doce años. Dividido en cuatro secciones, cada una explora con un tema cotidiano diversas áreas especializadas de la ciencia: nuestra tierra inquieta, maravillas naturales cerca de casa, maravillas físicas y materiales, viajes y transporte.

New maths frameworking: Step-Up Workbook 1.

Este libro asegura una transición sin problemas desde el nivel de enseñanza primaria Key Stage 2 a Key Stage 3 (enseñanza secundaria), las ilustraciones basadas en las preguntas hacen que los conceptos básicos sean fáciles de entender y recordar. Los temas del libro son: entendiendo los números (ordenando los números, contando), la suma, la resta, fracciones (mitades y cuartos), medidas de longitud y tiempo (midiendo en metros, midiendo en centímetros, la era digital), entendiendo las formas (lados y esquinas), multiplicación y división, medidas de peso, capacidad y tiempo.