Avaliação das alterações celulares nos ovócitos de fêmeas semi-ingurgitadas de carrapatos Rhipicephalus sanguineus (Acari: Ixodidae) decorrentes da exposição ao óleo de andiroba

Os carrapatos são considerados um dos mais importantes grupos dentre os artrópodes, por serem vetores de agentes patogênicos, que podem atacar animais, bem como o próprio homem. Dentre as diversas espécies de carrapato destaca-se a do Rhipicephalus sanguineus, pertencente à família Ixodidae e com ampla distribuição em todos os continentes. Atualmente esta espécie é considerada uma praga urbana, de grande importância médico-veterinária, que parasita principalmente o cão doméstico. Os ovários de carrapatos são considerados órgãos vitais para o sucesso biológico deste grupo de animais. Neste sentido, desenvolver pesquisas para se conhecer a ação de produtos naturais com reconhecida ação repelente ou acaricida, sobre o sistema reprodutor feminino de carrapatos, traria importantes informações para um melhor entendimento dos efeitos da ação destes produtos nos ovócitos desses ectoparasitas, contribuindo principalmente para o desenvolvimento de métodos de controle alternativos menos tóxicos para os organismos não-alvo e menos poluentes para o meio ambiente. Dessa forma, o presente estudo analisou as alterações morfofisiológicas causadas por diferentes concentrações do óleo de andiroba no ovário de fêmeas semiingurgitadas de carrapatos R. sanguineus

Avaliação das doses de cães submetidos a exames radiográficos de pulmão

This study aims to determine surface skin doses in dogs (with suspected pulmonary metastasis) submitted to chest X-rays using the technique of thermoluminescence dosimetry. Twenty seven exams from different dogs were performed at the Faculdade de Medicina Veterinária e Zootecnia da Universidade Estadual Paulista (FMVZ-UNESP/Botucatu). The doses were evaluated using thermoluminescent dosimeters of calcium sulphate doped with dysprosium (CaSO4:Dy) produced by the Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN). The pulmonary metastasis exams are carried out in three projections, one dorsal-ventral and two lateral-lateral. During the procedures the projection thicknesses and source-skin surface distances were registered. To simulate the dog phantom the dosimeters were positioned in a cubic simulator (30x30x30 cm) of polymethylmethacrylate (PMMA) filled with water and irradiated according to the parameters of projections with the X-ray energies ranging from 45 to 70 kV. To estimate the surface skin dose the dose-response curves were obtained for X-ray energies of 50 and 70 kV using the diagnostic X-ray beam machine of the Instruments Calibration Laboratory of IPEN for doses of 1.5, 2.0, 2.5, 3.5 and 4.0 mGy. The main difficulty of this work was the dog immobilization that is reflected in poor-quality diagnostic imaging and, consequently, demands the repetition of the exams, which contributes to the increase of the doses received by the animals being studied and the clinical staff. The doses evaluated in this type of procedure are between 0.43 and 4.22 mGy. This research has shown to be extremely important for the assessment of doses involved in veterinary diagnostic radiology procedures, and as a parameter in the individual monitoring of pet’s owners who assist the animal positioning and occupationally exposed workers of the Department of Veterinary Radiology

Indicação e avaliação de potenciais áreas para implementação de aterros sanitários municipais – Apuí/AM

About eighty percent of the Brazilian municipalities have their residues in environmentally unsuitable sites and socially insalubrious. In this context, in the year 2010, instituted the National Policy on Solid Residues, which aims to integrated management and management environmentally appropriate of solid residues in Brazil (JUCA, 2002).The problem of urban residues is related to its origin and production in the same way that the concept of inexhaustibility and reflexes of compromising the environment, especially the pollution of soil, air and water resources (LIMA, 1995). Seeking a better residues management in the municipality of Apuí / AM and continuing work already started, Plan Solid Residues Management of the Apuí / AM, this work came to propose a better environmental compliance in relation to solid residues compared to 12305/2010 law that established deadlines / timelines on some actions such as landfills and the consequent elimination of environmentally sound disposal of residues by 2014 and thus began a study of potential areas for the implementation of the landfill were evaluated taking into consideration technical aspects (soil, vegetation, climate, hydrography, etc), legislation and Google Earth imagery. The result of the work over the 6 areas proposed by the City Hall was the choice of AREA 4 as the most indicated for the implementation of the landfill for the city of Apuí, which has 18,325 inhabitants (IBGE, 2011); some considerations were made if a new area can be proposed by the city to implement the landfill

Padronização da técnica de PCR em tempo-real na avaliação da pluripotência de células-tronco mesequimais caninas

Este relatório final tem como objetivo apresentar as atividades desenvolvidas no período de janeiro/2009 a março/2010, pela aluna Thaila Isabel Wodewotzky, relativas ao projeto de conclusão de curso intitulado “Padronização da Técnica de PCR em tempo-real na Avaliação da Pluripotência de Células-tronco Mesenquimais Caninas”, para fins de obtenção do título de Bacharel em Ciências Biológicas. O referido projeto objetiva avaliar a quantificação e relevância dos níveis de expressão gênica do fator de transcrição Oct4 em CTM´s, por meio da padronização da técnica de PCR em tempo-real. Para tanto, o RNA total das CTM´s obtidas, isoladas e cultivadas a partir da medula óssea de cães foi extraído a partir da medula óssea de cães a fim de avaliar a quantificação e relevância dos níveis de expressão gênica do Oct4 por meio da utilização da técnica de PCR em tempo-real com transcrição reversa (RT-qPCR). O RNA total foi extraído e submetido à reação de transcriptase reversa, para a obtenção do cDNA. Posteriormente esse cDNA foi utilizado na padronização da técnica de qPCR utilizando primers desenhados a partir de sequências obtidas no genebank. . Como normalizador utilizou-se o RNA codificante de GAPDH.Verificou-se desempenho satisfatório dos primers para Otc4 na avaliação de CTM´s de cães. Também o RNAm do GAPDH foi adequado como normalizador. Dessa forma, esse sistema pode ser utilizado na realização de testes quantitativos utilizando amostras de células-tronco embrionárias caninas

Avaliação da tenacidade em um aço microligado, grau API, para aplicação em linhas dutoviárias de petróleo e gás

In order to study the mechanical properties of micro alloyed steel API 5L X70, a material used to manufacture pipes for pipeline transportation lines for use in oil and gas, a study was made of toughness, tensile strength, impact strength, hardness and microstructure steel. To perform these various tests were made where they can acquire the characteristics of the material. Were performed at the Faculty of Engineering in Guaratinguetá in the Department of Materials and Technology and the tensile tests, Charpy impact test, metallography and hardness testing of material API 5L X70, all tests were done with the help of technical laboratories. With these data can be an analysis of the material about his tenacity, his toughness and fragility, its hardness, its yield strength and its maximum voltage. After being asked the analyzes discussed the results showed that the micro alloyed steel API 5L X70 steel is a very tenacious, it absorbs impact energy of 300 Joules though without a break for the full body of evidence showing its tenacity

Avaliação das impedâncias em pontos de acupuntura

An experimental study in dogs was accomplished to evaluate impedances at the acupuncture points and the relative false points. According to the physics concepts and the Ohm’ Law, an electrical circuit was set up to determine the impedance. This circuit was introduced into three experiments in anesthetized dogs with needles at selected points. In the first two experiments, was applied an alternating tension with 200Hz frequency in the circuit and, in the last experiment, the frequency was increased to 10kHz, the tensions and electrical currents were measured between every pair of points for the all experiments. The impedances were calculated with the values obtained by experiments. The analysis of impedance values indicated that there is a coherent relation between the results and the applied frequencies in circuit, so just at high frequency condition admits the correct measurement of total impedance, therefore the last experiment gave the result which agreed with the theory, that the impedances at acupuncture points are lower than the impedances at false points

Avaliação da membrana P81 como agente ligante da técnica de difusão em filmes finos por gradiente de concentração (DGT) para determinação de mercúrio em sistemas aquáticos

Worldwide environmental degradation is an undesirable byproduct resulting from the increasing demand for natural resources. Water sources are suffering intense contamination since they usually receive a huge amount of domestic and industrial effluents - which are mostly wasted without proper treatment - inserting a large number of pollutants in the environment, heavy metals included. Mercury holds great toxicological importance because, under some physicochemical conditions in a water environment, Hg (II) ion turns into methylated compounds stemming from this element, such as methylmercury CH3Hg, which is highly toxic for the aquatic community in which bioaccumulation occurs. Nowadays passive sampling techniques are being developed to enable the analytical procedures which are applied in environmental monitoring. Diffusive gradients in thin-films technique (DGT) has been proven an interesting tool for the determination of labile metal species due to its in situ application. The DGT technique consists of a piston-like device on which the following series of agents is disposed: a binding agent (conventionally Chelex 100 resin), a diffusive agent, usually a polyacrylamide gel, and a membrane filter. Nevertheless, the agents conventinally used for this technique don't usually show satisfactory results in mercury sampling. The main goal of this study was to evaluate the phosphate-treated cellulose membrane (Whatman P 81), an alternative material, as binding agent in the DGT to determine labile mercury fractions in aquatic systems. In this context, we conducted a study of the behavior of this material in relation with system variables, pH and ionic strength. Afterwards we performed immersions of the DGT devices in real and enriched samples and in situ aiming the determination of mercury

A voz inicial dos professores sobre o processo de inclusão escolar num curso de formação continuada em EAD

The inclusive classroom, focused on individual differences, it is becoming more frequent and constant within mainstream schools. For this practice to be successful, there must be a different work by teachers, methodology, teaching procedures, adapted equipment and materials. Thus, the teacher who works directly with students with disabilities, find factors that facilitate and hinder this practice, making it essential to study of such factors, so we can understand and contribute to assessment and implementation of strategies to minimize the need that this pedagogical practice imposes. This varying needs, from the simple as using different materials to work in the classroom to the broader and more complex, as the reform in schools. Thus, these aimed to survey and analyze the conceptions of teachers on facilitating and hindering aspects of the schooling of students with disabilities in the regular classroom as well as check their training needs. The method used was ruled a quantitative approach to data collection was through a closed questionnaire containing 35 questions with Likert scale. The study included 904 teachers who underwent a course of Improvement in Inclusive Educational Practices, the same was applied when students entered the course, but had not had contact with the course content. The result showed that most teachers agree that all students with disabilities have behavior problem, dividing the assertion that the disabled student disrupts the class of the colleague who has a disability. It is also believed that in the view of most participants, students with disabilities are not able to study and that the inclusion of students with disabilities is hampered by...

Psicologia do trânsito e competência moral: uma intervenção junto a alunos de um curso de especialização

Pós-graduação em Educação - FFC

Avaliação dos aspectos da dieta alimentar de Pimelodus maculatus em dois importantes tributários do reservatório de Jurumirim, Alto Rio Paranapanema-SP

The species Pimelodus maculatus is one of the most abundant fish in many artificial reservoirs. Shows feeding plasticity and exploits all trophic levels in aquatic ecosystems. This study aimed to analyze and compare aspects of the diet of P. maculatus in two important tributaries (Taquari and Veados rivers) in the upper Paranapanema River system, and also investigate possible differences in the condition factor using its feeding habit as reference. Samplings were carried out every three months during 2011 and 2012 in two tributaries of Jurumirim's dam. After biometric analysis, fish were dissected to remove the stomachs that were fixed in formaldehyde 10% and conserved in alcohol 70%. The stomach content was analyzed under stereomicroscope and the food items were identified until the least possible taxonomic level. The diet was characterized based on the Alimentary Index (IAi). Feeding strategy and aspects of species autoecology were evaluated by the relation between abundance of prey-specific (Pi) and occurrence frequency (Fo) of the prey, and also was calculated the niche width of Levin (B). The condition factor was also established and values obtained in samples of both rivers were compared, correlating them with diet of the species

Avaliação da Taxa de Exposição em Exames Radiológicos Realizados em Procedimentos Cirúrgicos - Minimização dos Riscos em Indivíduos Ocupacionalmente Expostos

This paper consists in the evaluation of the exposure rate to ionizing radiation to which professionals working in surgical procedures which require radiological examinations are subjected. Were initially performed real-time readings of exposure rate within four distinct operating rooms during the execution of four surgical procedures that made use of fluoroscopy equipment (including three orthopedic surgeries, one in the shoulder, one in the arm, another for deployment of metal pin in the leg region, and a fourth for vascular procedure); in these surgeries were used ionization chamber detector and an electrometer. In order to check the values achieved, was made a re-evaluation of the distribution of the rate of exposure to radiation, from the surgical procedures, now with thermoluminescent dosimeters (TLDs). For this, thirty TLDs were distributed in the operating rooms, arranged in points of interest as occupation by professionals. The TLDs were prepared for thirty consecutive days, after which they were removed and replaced with new dosimeters not exposed yet. The dosimeters were subjected to reading of the rate of exposure; this procedure was repeated for four months without interruption. The quantification of the results sought primarily to convert the rate of exposure for equivalent dose rate, both in measurements with ionization chamber as in measurements with TLDs, in order to highlight the presence of the biological effect of ionizing radiation for comparisons within scientific context. Then, the results were plotted to establish the relationship between the values of equivalent dose and the distance to the central axis of the x -ray source, confirming the inverse square law for distance. Finally, the values were associated with the maximum limit recommended by the legislation for occupationally exposed individuals. The methodology for the analysis and quantification of the data in this work aims at implementing a work plan that meets ...

Avaliação do envolvimento do receptor dectina-1 na atividade fungicida e na produção de metabólitos do oxigênio por neutrófilos e monócitos humanos desafiados com Paracoccidioides brasiliensis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação dos efeitos ambientais nas propriedades do compósito de poliamida 6,6/fibras de carbono

This graduation work done study of polyamide 6.6/composite carbon fibres, since its processing, characterization of the main properties. Besides the influence of temperature, UV radiation, salt spray and moisture on the mechanical and viscoelastic behavior. To achieve this goal, the first composite was processed from the heat compression molding using known variables of the process and using the empirical method to find the best value for other parameters. The method processing molding was chosen because it common in composites processing in order to evaluate the influence of crystallinity of the properties that influence the mechanical and viscoelastic behavior laminates. From the obtained laminate specimens were evaluated in weathering, such as: in hygrothermal chamber, UV, salt spray and thermal shock. In another step, the effect produced by these constraints were evaluated by optical microscopy, ultrasound, dynamic mechanical analysis and vibration tests. This project was conducted at the Department of Technology and Materials of UNESP in Guaratingueta, where all the equipment and techniques for the implementation of this project met available. After the tests proved the applicability of the composite polyamide 6.6/carbon fibers in aeronautical applications with resistance the main climatic influences

Avaliação da influência do formato da porca e da pré-carga em juntas aparafusadas em tração

Many structures and mechanical equipment requires a union member, and the screw is one of the most used, the cost is low, it is versatile and easy to find on the market. The resulting pre-load assembly torque associated with the cyclical external load has great influence, so it will be studied carefully. This study analyzed some of these factors, as the format of nuts, and the washer material when they are incompatible with the bolt material can cause a reduction in life of the joint fatigue

O Serviço Social e a avaliação de impacto na gestão de programas e projetos empresariais

Pós-graduação em Serviço Social - FCHS