Temperamento divergente em bovinos está associado à alteração no amaciamento de carne e atividade inibitória de calpastatina

Embora muitos avanços tenham ocorrido no entendimento dos fatores que afetam a maciez, a incidência de carne dura ainda é significativa. Animais mais reativos apresentam carnes mais duras, porém o mecanismo que determina este efeito ainda não foi estabelecido. Presume-se que o maior estresse nos animais reativos aumente a atividade de calpastatina afetando o amaciamento post mortem. Com objetivo de testar esta hipótese, foram avaliadas correlações da força de cisalhamento (FC), temperamento e temperatura corporal obtida de noventa e seis novilhos Nelore castrados, com cerca de 20 meses de idade. O temperamento foi baseado nas variáveis velocidade de fuga (VF) e escore de tronco (ET) avaliadas 3 - 4 semanas antes do abate (Manejo 1) e 2 dias pré-abate (Manejo 2). A análise de componentes principais com as variáveis de temperamento obtidas em ambos os manejos resultou em dois índices: índice de temperamento (IT) e índice de habituação (IH). Estes índices apresentaram maior correlação com a FC do que a VF. O IH apresentou correlação negativa e positiva com FC e pH (P < 0,05), respectivamente. A temperatura retal foi positivamente correlacionada com IT e negativamente com FC. A termografia infravermelho de várias regiões corporais apresentou correlações baixas com IT ou FC. Dois grupos divergentes quanto à FC (extremos da população para FC), cada qual com 6 novilhos, foram comparados para variáveis de temperamento, expressão dos genes CAPN1, CAST, CAST1 e CAST2, e características de carne. O grupo de alta FC (AFC) apresentou maior (P< 0,05) VF, ET, IT e índice de temperamento com base na média da VF e ET (ITM). Os grupos de AFC e baixa FC (BFC) não diferiram (P > 0,05) para comprimento de sarcômero e pH final, mas tiveram diferenças no amaciamento post mortem, com diferenças em maciez que persistiram em todos os tempos de maturação (2, 16 e 30 dias post mortem; P < 0,05). O grupo AFC apresentou maior atividade inibitória de calpastatina total 48 h post mortem medida no M. triceps brachii (P < 0,05), embora diferenças não tenham sido observadas no M. longissimus lumborum (P > 0,05). No entanto, o grupo AFC teve no M. longissimus maior expressão de CAST do que o grupo de BFC (P < 0,05). Em conclusão, temperamentos divergentes têm impacto na atividade de calpastatina que influência o amaciamento post mortem.

Bienes muebles de mudéjares castellonenses. Siglo XV

Transcripción y estudio de 23 inventarios de bienes muebles de mudéjares castellonenses a través de los libros del Corte de Justicia para Castellón, Segorbe y Onda, fuente de muchos datos de diverso tipo sobre la vida de estos mudéjares y que generalmente se redactan post mortem para satisfacer deudas del fallecido. Se centra el análisis en la propiedad de animales de tiro y transporte, el trabajo de las fibras textiles y los elementos externos que los diferencian de los cristianos.

Ireland gets the new Trio Presidency off to a propitious start. CEPS Commentary, 30 August 2013

The Irish Presidency of the Council of the EU (January-June 2013) faced numerous challenges, not least of which was to negotiate the financial framework for the period 2014-2020 and the reform of the Common Agricultural Policy with the European Parliament, as well as the pressure to advance the banking agenda. Moreover, the fact that it was the start of a new Trio Presidency, the small size of the Irish administration and its fragile financial situation gave rise to some doubts as to how much it could achieve. Nevertheless, this post mortem on the Irish presidency finds that the Irish government approached the task with realism and optimism, a firm focus on results and the strong conviction that a good performance would enhance its reputation at home and in the EU. It is now for Lithuania and subsequently Greece, in the first half of 2014, to continue to tackle the remaining formidable challenges.

Étude du polymorphisme rs3846662 de l’HMGCR dans le cerveau et le système périphérique

Dans cette thèse, l’impact du polymorphisme rs3846662 sur l’épissage alternatif de la 3-hydroxy-3-méthylglutaryl coenzyme A réductase (HMGCR) a été investigué in vivo, chez des patients atteints d’hypercholestérolémie familiale (HF) ou de maladie d’Alzheimer (MA). Le premier manuscrit adresse la problématique de la normalisation de la quantification relative des ARNm par PCR quantitative. Les découvertes présentées dans ce manuscrit nous ont permis de déterminer avec un haut niveau de confiance les gènes de référence à utiliser pour la quantification relative des niveaux d’ARNm de l’HMGCR dans des échantillons de sang (troisième manuscrit) et de tissus cérébraux post-mortem (quatrième manuscrit). Dans le deuxième manuscrit, nous démontrons grâce à l’emploi de trois cohortes de patients distinctes, soit la population canadienne française du Québec et les deux populations nord américaines « Alzheimer’s Disease Cooperative Study (ADCS) » et « Alzheimer’s Disease Neuroimaging Initiative (ADNI) », que le génotype AA au locus rs3846662 confère à ces porteurs une protection considérable contre la MA. Les femmes porteuses de ce génotype voient leur risque de MA diminuer de près de 50% et l’âge d’apparition de leurs premiers symptômes retarder de 3.6 ans. Les porteurs de l’allèle à risque APOE4 voient pour leur part leurs niveaux de plaques séniles et dégénérescences neurofibrillaires diminuer significativement en présence du génotype AA. Enfin, les individus atteints de déficit cognitif léger et porteurs à la fois de l’allèle APOE4 et du génotype protecteur AA voient leur risque de convertir vers la MA chuter de 76 à 27%. Dans le troisième manuscrit, nous constatons que les individus atteints d’HF et porteurs du génotype AA ont, contrairement au modèle établi chez les gens normaux, des niveaux plus élevés de cholestérol total et de LDL-C avant traitement comparativement aux porteurs de l’allèle G. Le fait que cette association n’est observée que chez les non porteurs de l’APOE4 et que les femmes porteuses du génotype AA présentent à la fois une augmentation des niveaux d’ARNm totaux et une résistance aux traitements par statines, nous indique que ce génotype influencerait non seulement l’épissage alternatif, mais également la transcription de l’HMGCR. Comme une revue exhaustive de la littérature ne révèle aucune étude abondant dans ce sens, nos résultats suggèrent l’existence de joueurs encore inconnus qui viennent influencer la relation entre le génotype AA, l’épissage alternatif et les niveaux d’ARNm de l’HMGCR. Dans le quatrième manuscrit, l’absence d’associations entre le génotype AA et les niveaux d’ARNm Δ13 ou de protéines HMGCR nous suggère fortement que ce polymorphisme est non fonctionnel dans le SNC affecté par la MA. Une étude approfondie de la littérature nous a permis d’étayer cette hypothèse puisque les niveaux de HNRNPA1, la ribonucléoprotéine influencée par l’allèle au locus rs3846662, sont considérablement réduits dans la MA et le vieillissement. Il est donc proposé que les effets protecteurs contre la MA associés au génotype AA soient le résultat d’une action indirecte sur le processus physiopathologique.

Abundance of ostracoda in sediment cores CRP-1 and CRP-2

Sparse, poorly preserved late Oligocene (3 species) and early Miocene (4 species) ostracod faunas have been recovered from CRP-2A, while relatively more abundant Quaternary faunas occur in CRP-1 (24 species). All taxa are marine. No definitive age assignments can be made on the two older faunas, which are not considered to be in situ, although the taxa identified are not at variance with sediment ages determined on other grounds. The Oligocene ostracods (Lithostratigraphical Unit, LSU 9.4) suggest deposition in cold, relatively shallow, shelf waters with faunal connections to the Antarctic Peninsula and South America, while the Miocene fauna (LSU 5.1) is considered to be a cool-cold, deeper water (?outer shelf) association with faunal connections to both New Zealand and the Antarctic Peninsula. The Quaternary faunas are primarily from LSU 3.1 (carbonate-rich layer), and suggest deposition in very cold, relatively quiet water that was at least 100 m, and possibly 130-200 m deep. None of the taxa are known from pre-Pleistocene sediments, and all occur in modern Antarctic/sub-Antarctic regimes, predominantly from south of 60° S. Specimens in the "carbonate-rich layer" probably have suffered minor penecontemporaneous fractionation, while the fauna in LSU 2.2 has suffered more extensive post-mortem transportation and possible reworking (though not necessarily from pre-Quaternary sources).

Benthic foraminiferal assemblage counts from the Gulf of California

Extensive CO2 vents have been discovered in the Wagner Basin, northern Gulf of California, where they create large areas with lowered seawater pH. Such areas are suitable for investigations of long-term biological effects of ocean acidification and effects of CO2 leakage from subsea carbon capture storage. Here, we show responses of benthic foraminifera to seawater pH gradients at 74-207 m water depth. Living (rose Bengal stained) benthic foraminifera included Nonionella basispinata, Epistominella bradyana and Bulimina marginata. Studies on foraminifera at CO2 vents in the Mediterranean and off Papua New Guinea have shown dramatic long-term effects of acidified seawater. We found living calcareous benthic foraminifera in low pH conditions in the northern Gulf of California, although there was an impoverished species assemblage and evidence of post-mortem test dissolution.

Inqvisitionvm ad capellam domini regis retornatarvm, qvae in pvblicis archivis Scotiae adhvc servantvr, abbreviatio [1546-1700].

University at Buffalo copy lacks v. 1-2, 4.

Dissertationes in C. Julii Caesaris Commentarios.

Bd. 24. De elegantia Caesaris sive de commentariorum de b. G. et de b. c. differentiis animadversiones / O. Dernoscheck, 1903--Der Zug des Cimbern und Teutonen / A. Helbling, 1898--Unsere Armeesprache im Dienste der Caesar-Übersetzung / M. Hodermann, 1903--Cäsar, der Eroberer Galliens / R. Lange, 1896--Divico oder die von Caesar den Ost-Galliern und Süd-Germanen gegenüber Vertretene Politik, Lfg. I-III / H. von Müllinen, 1898-1901--Die Unterwerfung Galliens durch Cäsar verglichen mit der Bezwingung Frankreichs durch die deutsche Armee im Feldzuge 1870/71 / A. von Oertzen, 1904.

Ulyssis Aldrovandi ... De animalibus insectis libri septem : in quibus omnia illa animalia ... describuntur, eorum ivones ad visum ob omlos ponuntur, tandemg ; etiam natura, mores ac propietates ita declarantur ... : his adiunctus est index geninus capitum ... & verborum.

Contiene : De reliquis animalibus exanguibus : utpote De mollibus, crustaceis, Testaceis & 300 phythis libri quatuor post mortem eius editi : in quibus praedicta animalia omnia ... describuntur ... natura, mores ac propietates declarantur ...

Lancashire inquests, extents, and feudal aids.

Report of the Record Society for the year 1902/03 contained in v. 1, 1905/06 in v. 2, 1913/14 in v. 3.

Norfolk records.

Contents--v. 1. Collection of record-references derived from the official ms. indexes ... Edited by Walford D. Selby: Inquisitions post mortem or escheats. Licences and pardons (Alienation Office) Patent rolls. Placita de Banco (Common Pleas) Bills and answers (Exchequer, Queen's Remembrancer)--v. 2. Index to four series of Norfolk inquisitions: Tower series, Chancery or Rolls series, Exchequer series, Wards and Liveries or Court of Wards series ... Edited by Walter Rye.

Glutathione in Parkinson's disease

Thesis (Ph.D.)--University of Washington, 2016-03

GABAA receptor beta subunit mRNA expression in the human alcoholic brain

A competitive RT-PCR assay was used to quantify the expression of the GABA(A) receptor beta(1), beta(2) and beta(3) isoform mRNA transcripts in the superior frontal cortex and motor cortex of 21 control and 22 alcoholic cases. A single set of primers was designed that permitted amplification of all three transcripts and the internal standard simultaneously; differentiation of the individual transcripts was achieved by restriction enzyme digestion. Construction of a standard curve, using the internal standard and a concentration range of beta(2) cRNA-enabled quantitation of mRNA expression levels. No significant difference in mRNA expression was found between the control and alcoholic case groups in either the superior frontal or motor cortex for the beta(2) or beta(3) isoforms. A significant interaction was found between isoform and area, although, the two case groups did not partition on this measure. The interaction was due to a significant difference between superior frontal and motor cortex for the beta(3) isoform; this regional comparison was not significant for beta(2) mRNA. Age at death and post-mortem delay (PMD) had no significant effect on beta mRNA expression in either case group in either region. A beta(1) signal could not be detected in the RT-PCR assay. (C) 2004 Elsevier Ltd. All rights reserved.

The use of simultaneous H-1 & P-31 magic angle spinning nuclear magnetic resonance measurements to characterize energy metabolism during the conversion of muscle to meat

In order to investigate the potential of magic angle spinning nuclear magnetic resonance (MAS NMR) in the elucidation of post-mortem metabolism in muscle biopsies, simultaneous H-1 and (31)p MAS NMR measurements were made continuously on postmortem (20 min to 24 h) muscle longissimus samples from rabbits. The animals had either been or not been given adrenaline (0.5 mg kg(-1) 4 h pre-slaughter) to deplete stores of muscle glycogen. The intracellular pH was calculated from H-1 spectra, and the post-mortem rate of formation of lactate was followed and quantified. Comparison of measurements made on muscle samples from rabbits treated with adrenaline with measurements made on muscle samples from untreated' rabbits revealed significant effects of adrenaline treatment on both pH (pH24 h = 6.42 vs. pH24 It = 5.60) and formation of lactate (16 mmol g(-1) vs. 65 mmol g(-1)). The P-31 NMR spectra were used to follow the rate of degradation of ATP and phosphocreatine. The present study clearly shows that MAS NMR has potential for the study of post-mortem energy metabolism.

Fetal echocardiography: are we getting it right?

Objective To determine the diagnostic accuracy of prenatal fetal echocardiography. Methods The study was a retrospective chart review of 190 consecutive patients over a 3-year period from November 1998 to February 2002 of all women referred to the Maternal Fetal Medicine unit, Mater Mothers Hospital, for fetal echocardiography. The prenatal diagnosis was compared with the postnatal diagnosis made by postnatal echocardiography, surgical findings or post-mortem. The accuracy of prenatal diagnosis was described on a predetermined 4-point scale. Results Of the 89 patients, for whom complete diagnostic follow-up was available, there was complete agreement between the prenatal and postnatal diagnosis in 63 cases, minor discrepancies in 25 cases and major disagreement in 1 case. Conclusions In experienced hands, fetal echocardiography is accurate and allows medical staff and patients information in order manage a pregnancy appropriately. Copyright (C) 2004 John Wiley Sons, Ltd.