The use of simultaneous H-1 & P-31 magic angle spinning nuclear magnetic resonance measurements to characterize energy metabolism during the conversion of muscle to meat


Autoria(s): Bertram, H. C.; Whittaker, A. K.; Andersen, H. J.; Karlsson, A. H.
Contribuinte(s)

Christopher Smith

Data(s)

01/01/2004

Resumo

In order to investigate the potential of magic angle spinning nuclear magnetic resonance (MAS NMR) in the elucidation of post-mortem metabolism in muscle biopsies, simultaneous H-1 and (31)p MAS NMR measurements were made continuously on postmortem (20 min to 24 h) muscle longissimus samples from rabbits. The animals had either been or not been given adrenaline (0.5 mg kg(-1) 4 h pre-slaughter) to deplete stores of muscle glycogen. The intracellular pH was calculated from H-1 spectra, and the post-mortem rate of formation of lactate was followed and quantified. Comparison of measurements made on muscle samples from rabbits treated with adrenaline with measurements made on muscle samples from untreated' rabbits revealed significant effects of adrenaline treatment on both pH (pH24 h = 6.42 vs. pH24 It = 5.60) and formation of lactate (16 mmol g(-1) vs. 65 mmol g(-1)). The P-31 NMR spectra were used to follow the rate of degradation of ATP and phosphocreatine. The present study clearly shows that MAS NMR has potential for the study of post-mortem energy metabolism.

Identificador

http://espace.library.uq.edu.au/view/UQ:73150

Idioma(s)

eng

Publicador

Blackwell Publishing Ltd

Palavras-Chave #Food Science & Technology #Glycogen Status #Lactate #Magic Angle Spinning #Nmr #Ph #Phosphorus Metabolites #Water-holding Capacity #Human Skeletal-muscle #M-longissimus-dorsi #High-resolution #Postmortem Metabolism #H-1-nmr Spectroscopy #Nmr-spectroscopy #Intracellular Ph #Bovine Muscles #Rat-liver #Lactate #C1 #250104 Chemical Spectroscopy #670102 Meat products
Tipo

Journal Article