Onnistuneen uuden tuotteen kehittäminen ja hallinta - Tapaustutkimus: Tekstiilialan yritys

Tuotesuunnittelu on yrityksen selviämisen kannalta erittäin tärkeä toiminto. Menestynyt tuotesuunnittelu vaatii selkeän tuotestrategian, laadukkaasti suoritetun tuotesuunnitteluprosessin ja tarvittavienresurssien yhdistämistä sekä uuden tuotteen tuottojen hallintaa myynnin seurannalla. Kohdeyrityksessä uusien tuotteiden suunnittelun ja hallinnan tärkeys on huomattu ja niiden kehittämiseksi yritys on lisäämässä panostustaan. Tämä tutkimuspyrkii tuomaan kohdeyrityksen tietoon tuotesuunnitteluun ja uuden tuotteen hallintaan vaikuttavat menestystekijät sekä tuomaan esille näitä toimintoja tukevia kehittämismahdollisuuksia. Tutkimus on normatiivinen tapaustutkimus, jossa teoria on koottu tieteellisistä, lähinnä empiirisistä tutkimuksista koostuvista artikkeleista. Yrityksen tuotesuunnitteluun liittyvät prosessit ja toiminnot on selvitetty puolistrukturoiduilla haastatteluilla sekä havainnoimalla. Tutkimuksessa havaittiin selkeitä eroja uuden tuotteen hallinnan menestystekijöiden ja yrityksen toimintojen välillä. Tuotesuunnitteluprosessin uudelleen organisointi, toimitusketjun hallinta ja tuotekohtainen kustannuslaskenta ovat asioita, joita kehittämällä yritys voi mahdollisesti parantaa uusien tuotteiden elinkaaren aikaista hallintaa ja taloudellista onnistumista sekä lisätä yrityksen kannattavuutta.

Organisaation tiedonhallinta - Kodifiointi ja henkilöltä henkilölle -strategia

Työssä tarkastellaan kahta vaihtoehtoista tiedonhallinta-strategiaa; kodifiointia ja henkilöltä henkilölle -strategiaa. Molempia strategioita analysoidaan, ja pohditaan organisaation päätöksentekoperusteita strategian valinnalle. Kodifiointi-strategiaa tutkitaan henkilöltä henkilölle -strategiaa perusteellisemmin. Strategioita tutkitaan muun muassa SWOT -analyysin avulla, sekä organisaation että käsiteltävän tiedon erityispiirteitä silmällä pitäen. Työssä tutustutaan myös tiedonhallinta-strategian suunnitteluun ja toteutukseen, sekä hahmotellaan tiedonhallinta-strategian evoluutiota. Myös tietoa ja sen luonnetta tarkastellaan yhtenä organisaation tärkeimmistä resursseista.

Semantic Web applications: a framework for industry and business exploitation – What is needed for the adoption of the Semantic Web from the market and industry

In recent years, Semantic Web (SW) research has resulted in significant outcomes. Various industries have adopted SW technologies, while the ‘deep web’ is still pursuing the critical transformation point, in which the majority of data found on the deep web will be exploited through SW value layers. In this article we analyse the SW applications from a ‘market’ perspective. We are setting the key requirements for real-world information systems that are SW-enabled and we discuss the major difficulties for the SW uptake that has been delayed. This article contributes to the literature of SW and knowledge management providing a context for discourse towards best practices on SW-based information systems.

Semantics-supported cooperative learning for enhanced awareness

Awareness is required for supporting all forms of cooperation. In Computer Supported Collaborative Learning (CSCL), awareness can be used for enhancing collaborative opportunities across physical distances and in computer-mediated environments. Shared Knowledge Awareness (SKA) intends to increase the perception about the shared knowledge, students have in a collaborative learning scenario and also concerns the understanding that this group has about it. However, it is very difficult to produce accurate awareness indicators based on informal message exchange among the participants. Therefore, we propose a semantic system for cooperation that makes use of formal methods for knowledge representation based on semantic web technologies. From these semantics-enhanced repository and messages, it could be easier to compute more accurate awareness.

Neurites regrowth of cortical neurons by GSK3b inhibition independently of Nogo Receptor 1

Lesioned axons do not regenerate in the adult mammalian central nervous system, owing to the overexpression of inhibitory molecules such as myelin-derived proteins or chondroitin sulphate proteoglycans. In order to overcome axon inhibition, strategies based on extrinsic and intrinsic treatments have been developed. For myelin-associated inhibition, blockage with NEP1-40, receptor bodies or IN-1 antibodies has been used. In addition, endogenous blockage of cell signalling mechanisms induced by myelin-associated proteins is a potential tool for overcoming axon inhibitory signals. We examined the participation of glycogen synthase kinase 3 (GSK3) and ERK1/2 in axon regeneration failure in lesioned cortical neurons. We also investigated whether pharmacological blockage of GSK3 and ERK1/2 activities facilitates regeneration after myelin-directed inhibition in two models: i) cerebellar granule cells and ii) lesioned entorhino-hippocampal pathway in slice cultures, and whether the regenerative effects are mediated by Nogo Receptor 1 (NgR1). We demonstrate that, in contrast to ERK1/2 inhibition, the pharmacological treatment of GSK3 inhibition strongly facilitated regrowth of cerebellar granule neurons over myelin independently of NgR1. Lastly these regenerative effects were corroborated in the lesioned EHP in NgR1 -/- mutant mice. These results provide new findings for the development of new assays and strategies to enhance axon regeneration in injured cortical connections.

Julkisen asiantuntijaorganisaation johtaminen. Case: Lappeenrannan Seudun Elinkeino ja Matkailu Oy

Tutkimuksen päätavoitteena on selvittää kyselytutkimuksen avulla julkisen asiantuntijaorganisaation kohtaamia johtamisen haasteita. Johtamistyötä tarkastellaan organisaation henkilöstön, julkisen omistajuuden ja paikallisten yhteistyöverkostojen näkökulmasta. Teoriaosuudessa tarkastellaan asiantuntijaorganisaatiota yleisesti sekä julkisen asiantuntijaorganisaation ominaispiirteitä, asiantuntijoiden johtamista ja johtamisen työkaluista tulosjohtamista sekä tasapainotetun mittariston mallia. Empiirisessä osuudessa teoriaa sovelletaan käytäntöön case-organisaation kautta. Tutkimuksen tuloksena todetaan, että julkinen omistajuus tuo yhtiön toimintaan vakauden ja uskottavuuden lisäksi päätöksentekoa hidastavaa byrokratiaa. Alueellisen yhteistyön kautta voidaan parantaa alueen painoarvoa, projektityöskentelyä ja rahoituksen saantia, sekä tuottaa palveluja kustannustehokkaasti. Henkilöstö odottaa johtajaltaan riittävää palautetta, kuuntelemisen taitoa, sekä vapautta itsenäiseen työskentelyyn oman tehtäväkentän alueella.

Efficacy of tumor necrosis factor inhibitors in patients with ankylosing spondylitis

Objectives: This study aims to investigate the efficacy of tumor necrosis factor-alpha blockers such as infliximab, etanercept, and adalimumab in the treatment of ankylosing spondylitis. Patients and methods: The outcome of tumor necrosis factor-alpha blocker treatment was analyzed retrospectively in 59 patients with ankylosing spondylitis who were being treated in our clinic during last nine years. The patients' Assessment of SpondyloArthritis International Society (ASAS) 20 and ASAS 40 response rates, adverse drugs effects, and treatment compliance were evaluated. Results: ASAS 20 response was achieved by 89.8% of the patients in the third month, and by 93.2% in the sixth month. ASAS 40 response was achieved by 61% of the patients in the third and sixth month. No statistically significant difference was detected between the three tumor necrosis factor-alpha blockers with regards to the ASAS 40 response rates. Mild infections, observed in 31 of the patients, were the most common side effects. Serious side effect was observed in only one patient. The number of patients who withdrew from the treatment for various reasons was six.

Análise da nova classificação de laudos de densitometria óssea

OBJETIVO: Comparar a nova normatização de laudos densitométricos de coluna lombar proposta pela International Society for Clinical Densitometry (ISCD) em 2005 com a classificação rotineiramente usada da Organização Mundial da Saúde (OMS) desde 1994. MATERIAIS E MÉTODOS: Foram analisados 200 exames de densitometria óssea da coluna lombar realizados na Universidade Católica de Brasília e no Hospital das Forças Armadas. Os critérios de inclusão foram: sexo feminino, idade mínima de 20 anos e máxima de 49 anos, e ausência de alterações morfológicas na coluna lombar visualizadas no exame densitométrico. Como critério de exclusão, foram consideradas as mulheres com mais de 50 anos ou em menopausa. RESULTADOS: Pela classificação da OMS, obtivemos 29 pacientes com osteoporose na coluna lombar, 76 com osteopenia e 95 em níveis de normalidade. Entretanto, pela nova classificação da ISCD 2005, apenas 32 pacientes foram classificados em valores "abaixo do estimado para a faixa etária" e 162, em valores "dentro do estimado para a faixa etária". CONCLUSÃO: Importante diferença foi encontrada na classificação dos resultados densitométricos ao se confrontar a classificação tradicional da OMS e a nova classificação proposta pela ISCD, o que ressalta a importância da divulgação da classificação entre os médicos que realizam e os que solicitam os exames, para a correta interpretação, explicação e orientação ao paciente.

Microparticles from stored red blood cells induce thrombin generation

Background: Microparticles are small phospholipid vesicles of <1 lm shed in blood flow by various cell types including red blood cells. Erythrocyte-derived microparticles (EMPs) accumulate in erythrocyte concentrates (ECs) during their storage time. EMPs are considered as part of storage lesion and as their exact role is not elucidated, they could be involved in these clinical outcomes. Aims: The aim of this study is to evaluate the impact and implication of EMPs isolate from ECs on coagulation. Methods: EMPs were first isolated from erythrocyte concentrates by centrifugation and counted by flow cytometry. Using a calibrated automated thrombogram, EMPs were then added to different type of plasmas in order to evaluate the potential of thrombin generation. Results: We demonstrate that EMPs isolated from ECs are capable to accelerate and amplify thrombin generation in presence of a low exogenous tissue factor concentration, thanks to their negatively charged membrane necessary for the assembly of coagulation complexes. Interestingly, in the absence of exogenous tissue factor, EMPs are also able to trigger thrombin generation. In addition, thrombin generation induced by EMPs is not affected by the presence of anti-TF antibodies. Finally, thrombin generation induced by EMPs is not affected by using plasma samples deficient in factor VII, XI or XII. However, thrombin generation is reduced in plasma deficient in factor VIII or IX and is completely abolished in plasma deficient in factor X, V or II. No thrombin generation was observed in plasma samples without EMPs. Summary/conclusion: Several studies have shown a link between storage time of blood products and post transfusion complications. We provide evidence that EMPs accumulated during storage of erythrocyte concentrates were not only able to accelerate and support thrombin generation in plasma in presence of a low exogenous tissue-factor concentration, but also to trigger thrombin generation in absence of exogenous TF. The impact of those transfused EMs is unknown on recipients, nevertheless it could be hypothesized that under certain circumstances, transfused EMPs could be involved in thrombin generation and could be linked to adverse clinical outcome. Further work is needed to determine whether procoagulant EMPs transfused with erythrocyte concentrate may account for some of the complications occurring after red blood cell transfusion, and more particularly after transfusion of ''older''stored blood, rich in EMPs.