Rodococose equina: uma revisão com ênfase na fisiopatogenia e tratamento

The rhodococcosis affects humans and animals. Equine are the most important species for rhodococcosis, which is considered the most debilitating disease in the creation of foals, leading to a mortality rates greater than 50% in this category. The disease is caused by Rhodococcus equi, a bacteria considered as a soil-born opportunistic microorganism, intracellular and ubiquitous. The disease in horses is manifested mainly in the form of pyogranulomatous pneumonia and less often in the form of enteric disorders and / or joint disorders. Transmission occurs mainly by ingestion of contaminated food and water, and inhalation in contaminated environment. The organism has mechanisms of evasion of the immune system, maintain viable in inside phagocytic cells, and induces piogranulomatous infections, leading to lesions of difficult treatment using conventional antimicrobials. Lipophilic drugs with good intracellular activity are required to successful treatment. The conventional treatment for foals is based on the combination of erythromycin and rifampin. However, there is a growing concern about the emergence of resistant strains, which makes increasingly studies on the development of alternative antimicrobials for therapy

Pesquisa de genes de resistência do M. tuberculosis a isoniazida e rifampicina por sequenciamento e pela tecnologia de DNA-STRIP

Tuberculose (TB), causada por Mycobacterium tuberculosis, é uma das doenças infecciosas que mais causam mortes. Estima-se que mais de dois bilhões de pessoas estejam infectadas no mundo. O tratamento da TB consite em associação de fármacos, isoniazida, rifampicina, pirazinamida e etambutol, nos primeiros 2 meses e 4 meses de isoniazida e de rifampicina. Internacionalmente, são consideradas cepas multi resistentes (MDR), as que apresentam resistência simultânea a isoniazida e a rifampicina. A rápida detecção de resistência é essencial para o controle e tratamento da TB, reduzindo, assim, o custo do tratamento e a transmissão da doença. Neste projeto, os isolados já identificados fenotipicamente como resistentes a isoniazida e/ou rifampicina, foram submetidos ao sequenciamento de Sanger para pesquisa de 3 genes relacionados a resistência a isoniazida (katG, inhA e ahpC) e 1 gene de resistência a rifampicina (rpoB). Foi realizada uma comparação destes genes mutados para a resistência utilizando o novo teste desenvolvido pela Biomérieux, denominado GenoType® MTBDRplus, que se baseia na tecnologia DNA-STRIP. Através deste novo teste, foi observada mutação em 22 isolados clínicos de M. tuberculosis para genes de resistência a isoniazida e/ou rifampicina, sendo 4 provenientes do MS e 18 de SP. Já pelo sequenciamento genético foi observada mutação em 24 isolados para genes de resistência a isoniazida e/ou rifampicina, sendo 6 provenientes do MS e 18 de SP. Portanto, através do sequenciamento de Sanger, foi possível detectar um número maior de isolados mutados e mais mutações quando comparado ao teste GenoType® MTBDRplus. Isso acontece porque na técnica de sequenciamento, um fragmento do gene como um todo é analisado e no caso do teste GenoType® MTBDRplus, é verificada apenas a ausência ou presença das mutações mais frequentes descritas na literatura, além de não ser analisado o gene ahpC. A grande ...

Modelos matemáticos e computacionais em dengue

This work was developed starting the study of traditionals mathematical models that describe the epidemiology of infectious díseases by direct or indirect transmission. We did the classical approach of equilibrium solutions search, its analysis of stability analytically and by numerical solutions. After, we applied these techniques in a compartimental model of Dengue transmission that consider the mosquito population (susceptible vector Vs and 'infected vector VI), human population (suseeptíble humans S, infected humans I and recovered humans R) and just one sorotype floating in this population. We found the equilibrium solutions and from their analises, it was possible find the reprodution rate of dísease and which define if the disease will be endemic or not in the population.- ext, we used the method described a..~, [1] to study the infíuence of seasonalíty at vírus transmission, when it just acts on one of rates related with the vector. Lastly, we made de modeling considering the periodicity of alI rates, thereby building, a modeI with temporal dependence that permits to study periodicity of transmission through of the approach of parametrical ressonance and genetic algorithm

Epidemiologia da infecção por Leptospira spp. em áreas rurais nos biomas brasileiros

Pós-graduação em Medicina Veterinária - FCAV

Expressão gênica de MMP-2 e 9, TIMP-1 e 2, ATM, TP53, VEGF, COX-2 e CDH-1 no TVT canino

Pós-graduação em Medicina Veterinária - FCAV

Controle quimioterápico da mosca Stomoxys calcitrans (Insecta: Muscidae)

Pós-graduação em Medicina Veterinária - FCAV

Validação de métodos nociceptivos elétrico, mecânico e térmico para avaliação de dor de equinos tratados com metadona associada à detomidinaou acepromazina

Pós-graduação em Anestesiologia - FMB

Expressão dos genes MDR-1, TP53, BCL-2 e BAX em tumor venéreo transmissível canino e sua relação com a agressividade e resposta à terapia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morfologia do estômago do cervo-do-pantanal (Blastocerus dichotomus, Illiger 1815)

Based on the lack of information regarding the morphology of marsh deer, this work aims to describe some morphological aspects of the gastric chamber in this species, collaborating with future investigations, mainly related to rational handling in this cervid. This work aimed to describe the morphology of the gastric chamber of the marsh deer, characterizing the external and internal macroscopical details and the microscopical architecture of these structures by light microscopy. Macroscopically, the marsh deer stomach is formed by the rumen, reticulum, omasum and abomasum similar to the domestic ruminants. Microscopically, rumen and abomasum are similar to the domestic ruminants. The reticulum and the omasum, however, present specific characteristics such as keratin on the top of the reticulum, small epithelial projections and omasum folds covered with discrete papillae.

Desenvolvimento e aplicação do Elisa indireto com a nucleoproteína recombinante (NPR) do vírus da doença de Newcastle

Pós-graduação em Medicina Veterinária - FCAV

Soroprevalência da brucelose bovina em frigoríficos com diferentes níveis de serviço de inspeção no estado de São Paulo

Pós-graduação em Medicina Veterinária - FMVZ

Malaria and Vascular Endothelium

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Eficácia e efeitos hemodinâmicos da anestesia raquidiana com ropivacaína isobárica, hipobárica ou hiperbárica seletiva em cães anestesiados com isofluorano

Pós-graduação em Ciência Animal - FMVA

Speech-language therapy for treatment of dysphagia and dysphonia in systemic granulomatous diseases: granulomatous diseases: dysphagia and dysphonia treatments

The granulomatous lesions are frequently founded in infectious diseases and can involve the larynx and pharynx and can cause varying degrees of dysphonia and dysphagia. There is still no systematic review that analyzes effectiveness of speech therapy in systemic granulomatous diseases. Research strategy: A systematic review was performed according to Cochrane guideline considering the inclusion of RCTs and quasi-RCTs about the effectiveness of speech-language therapy to treat dysphagia and dysphonia symptoms in systemic granulomatous diseases of the larynx and pharynx. Selection criteria: The outcome planned to be measured in this review were: swallowing impairment, frequency of chest infections and voice and swallowing symptoms. Data analysis: We identified 1,140 citations from all electronic databases. After an initial shift we only selected 9 titles to be retrieved in full-text. After full reading, there was no RCT found in this review and therefore, we only described the existing 2 case series studies. Results: There were no randomized controlled trials found in the literature. Therefore, two studies were selected to be included only for narratively analysis as they were case series. Conclusion: There is no evidence from high quality studies about the effectiveness of speech-language therapy in patients with granulomatous diseases of the larynx and pharynx. The investigators could rely in the outcomes suggested in this review to design their own clinical trials.

Conscientizar para o bem-estar animal: posse responsável

The community awareness on responsible ownership associated with public policies is key to the promotion of animal welfare. Most cities have a large population of dogs and cats wandering causing serious health problems and abuse. This project aimed to guide and inform the population aimed at animal welfare with the simple and direct approach to issues related to responsible ownership, management, nature conservation and zoonoses. The methodology developed to approach the project in two modules: the I and II, along public and private Elementary and High Schools in Araçatuba. Module I (Mod.I) is basedon the use of puppet theater and musicals, considering the target audience children 3-10 years and the second module (Mod 2) consists of presentations of lectures by undergraduate and graduate course of Veterinary Medicine FMVA UNESP campus Araçatuba, being the target audience of High School teenagers. Data collection proceeded by questionnaires, with questions regarding the care and practices to pets. The results revealed that there is a lack of information about animal welfare practice.